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Gene information
General information
TC011667 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 16143647..16165734
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011667
    iBeetle screen iB_05074

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva anterior decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
    head appendages partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head capsule not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment anterior number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011667 RA
      MAEGFAKKC FGLAETRNY KLNEVGNVA DLNDYAPKS 
      IKSRMAANI TQDLGINET NETSYQESG YNQAESIII 
      TIILVIIIV GTIVGNILV CVAVCLVRK LRRPSNYLL 
      VSLAVSDLC VALLVMPMA MIYEIKGRW IFGEVVCNL 
      WVSFDVLSC TASILNLCM ISVDRYYAI TKPLEYGVK 
      RTPKRMILW VIVVWCFAA CISLPPLLI IGNEHSEKE 
      ENGDINKEV CLVSQDFGY QLYATLCSF YIPLTVMMV 
      VYYKIFRAA RKIVMEEKR AQSHLESHC YLEISVKNG 
      GGPPENKIS SQSPSPANP SRINHRSSS ASTNTMCSL 
      DKSSLGRCF KSRHSNESQ CPMLSNKAP IKQTTKDKQ 
      QKSSLLASM KSSPNTNST NQKKLRFQL AKERKASTT 
      LGIIMSAFT ICWLPFFVL ALVRPFLKT THMLKTLGS 
      LFLWLGYAN SLFNPIIYA TLNRDFRKP FQAILYFRC 
      GSLNHMMRE EFYHSQYGD PDHHISTRC HLDYEEGVE 
      YIEAEGDEV KTPEINAAH ESFL
    CDS sequence
    • >TC011667 RA
      atggctgaa ggttttgcc aagaagtgt tttggactg 
      gcagaaacg cggaactat aaattaaat gaagttgga 
      aatgtagcg gacctcaat gactatgcc ccaaaatca 
      ataaaatct cgtatggcg gccaatata actcaagat 
      ttgggaatc aacgaaaca aatgaaacc tcttatcaa 
      gaatccgga tataaccaa gccgagagc atcatcata 
      acgatcatc ttagtgata ataatagtt ggaacaatc 
      gtgggcaat attttggtg tgtgtggct gtgtgtctg 
      gtgcgaaag ctgcgccgt ccttccaac tacctcctg 
      gtgagtttg gctgtgagc gacctctgc gtggcgctc 
      cttgtcatg cccatggcc atgatctac gagattaaa 
      ggccggtgg atctttggc gaagtggtc tgtaacttg 
      tgggtgtct ttcgacgtc ctcagctgc acagcttct 
      attctcaac ttgtgcatg atcagtgtg gatcgctac 
      tacgccatt accaagccg ctggaatac ggcgtcaaa 
      cgtactcct aagagaatg attctctgg gtgatcgtg 
      gtgtggtgt ttcgctgcc tgtatttcg cttcctcca 
      cttctcatc atcggaaat gaacattcc gagaaggaa 
      gagaacggc gatataaat aaagaagtc tgtttagtt 
      tcgcaggat ttcggttat cagttatat gcgactttg 
      tgtagtttt tatataccg ttgactgtg atgatggtg 
      gtgtattat aaaatattc agggctgcg aggaaaatt 
      gttatggaa gagaaaagg gcccaaagt catctagaa 
      tcgcattgt tatctagag attagtgtg aagaatggc 
      ggaggacct ccagaaaat aagatttct agtcaatca 
      ccttcaccg gctaatccc tcgagaatt aatcataga 
      tctagttcg gctagcaca aacaccatg tgcagtctg 
      gacaagagc agcttaggt cgttgcttt aaatctcga 
      catagcaac gaatcccag tgtcccatg ctaagtaac 
      aaagcaccg atcaaacaa acgaccaaa gataaacaa 
      caaaagtcc agccttctg gcatcgatg aaatcctcg 
      ccgaacacc aactccaca aatcaaaag aagcttcgc 
      tttcaacta gcgaaggaa cggaaagcc agtaccact 
      ttagggatc attatgagt gcttttacg atttgctgg 
      ttgccgttt ttcgtgttg gccttagta aggccgttc 
      ttaaagact actcatatg ctgaagact ttaggaagt 
      cttttcttg tggttaggt tatgctaat agtttattt 
      aatccgatt atttacgcg acgctaaat cgggacttc 
      cggaagcct ttccaagcc attctctat tttcggtgt 
      ggcagttta aaccacatg atgagagaa gaattttac 
      catagccaa tatggagat ccagaccat catatttcc 
      acgagatgt cacttggat tacgaagag ggagttgaa 
      tatattgaa gctgaagga gatgaagtc aagactcct 
      gaaataaat gcagcacac gaatcgttt ctatga
    mRNA sequence
    • >TC011667 RA
      cgtttcgtg agcatgctg aactgcgtc gcccccgct 
      caggatttg ctccttccg ttcaggcgc tgagaggaa 
      aaatcggtg atttcgggg gaacaccga ggtgaggtt 
      ttgtcgtaa tggtgcgga agtaaacga tcaaaaaac 
      tggtgctgg aaatttggt tgccaaagt tacctacgt 
      ttctctcac aacaaaaat ccacagtga acgtgtaaa 
      ttaatgtgt tttgacgat accgccaga gtatggatt 
      ggtttcctc taaattagc acgtaatta aactgccac 
      aacaaaggg aacgattct atgtacagc cggtggcat 
      cgcgtaatc ggatgcatc acatcaagc ggatgcacg 
      ctaacatct ccggaaatg gctgaaggt tttgccaag 
      aagtgtttt ggactggca gaaacgcgg aactataaa 
      ttaaatgaa gttggaaat gtagcggac ctcaatgac 
      tatgcccca aaatcaata aaatctcgt atggcggcc 
      aatataact caagatttg ggaatcaac gaaacaaat 
      gaaacctct tatcaagaa tccggatat aaccaagcc 
      gagagcatc atcataacg atcatctta gtgataata 
      atagttgga acaatcgtg ggcaatatt ttggtgtgt 
      gtggctgtg tgtctggtg cgaaagctg cgccgtcct 
      tccaactac ctcctggtg agtttggct gtgagcgac 
      ctctgcgtg gcgctcctt gtcatgccc atggccatg 
      atctacgag attaaaggc cggtggatc tttggcgaa 
      gtggtctgt aacttgtgg gtgtctttc gacgtcctc 
      agctgcaca gcttctatt ctcaacttg tgcatgatc 
      agtgtggat cgctactac gccattacc aagccgctg 
      gaatacggc gtcaaacgt actcctaag agaatgatt 
      ctctgggtg atcgtggtg tggtgtttc gctgcctgt 
      atttcgctt cctccactt ctcatcatc ggaaatgaa 
      cattccgag aaggaagag aacggcgat ataaataaa 
      gaagtctgt ttagtttcg caggatttc ggttatcag 
      ttatatgcg actttgtgt agtttttat ataccgttg 
      actgtgatg atggtggtg tattataaa atattcagg 
      gctgcgagg aaaattgtt atggaagag aaaagggcc 
      caaagtcat ctagaatcg cattgttat ctagagatt 
      agtgtgaag aatggcgga ggacctcca gaaaataag 
      atttctagt caatcacct tcaccggct aatccctcg 
      agaattaat catagatct agttcggct agcacaaac 
      accatgtgc agtctggac aagagcagc ttaggtcgt 
      tgctttaaa tctcgacat agcaacgaa tcccagtgt 
      cccatgcta agtaacaaa gcaccgatc aaacaaacg 
      accaaagat aaacaacaa aagtccagc cttctggca 
      tcgatgaaa tcctcgccg aacaccaac tccacaaat 
      caaaagaag cttcgcttt caactagcg aaggaacgg 
      aaagccagt accacttta gggatcatt atgagtgct 
      tttacgatt tgctggttg ccgtttttc gtgttggcc 
      ttagtaagg ccgttctta aagactact catatgctg 
      aagacttta ggaagtctt ttcttgtgg ttaggttat 
      gctaatagt ttatttaat ccgattatt tacgcgacg 
      ctaaatcgg gacttccgg aagcctttc caagccatt 
      ctctatttt cggtgtggc agtttaaac cacatgatg 
      agagaagaa ttttaccat agccaatat ggagatcca 
      gaccatcat atttccacg agatgtcac ttggattac 
      gaagaggga gttgaatat attgaagct gaaggagat 
      gaagtcaag actcctgaa ataaatgca gcacacgaa 
      tcgtttcta tgatcgaac gattagtta acaagtggg 
      aggacaaag tcttctttt ccaaccgaa aaatagtga 
      cgtgttttc taataaata aaaaacagt ttggttttc 
      tttcgtttc ttatgactg aaattgaaa attccaatc 
      gatccacac cctgggaac aataataat ctggtcgca 
      tcactttta tttttgtaa gagtgtata aagcctaaa 
      aatatcagt cgtgtttca gctgacttc catactaaa 
      cgagctttt gttttcttt gaattataa aaatctgac 
      tcgcaggat gaatctata atactcgta tgtgtattg 
      gaaaatgta gaaataaca aaaaaacat taaaaggat 
      gtgtgctat gcttttatg atttcttga gaatagatt 
      caagcgtta cttcgaaaa gttacaata attaacgtc 
      taattaaaa caatccggt atttatcta cggaatagt 
      atattgaac aaattattt ctctcaaag aatattaga 
      acaggaaaa attggattt tttttctta attattgat 
      ttttattaa ctgggtaaa attctattg caatgtggg 
      atttcgatg cagttcatc gaatagaaa agttttatt 
      aaattccgt cacggagaa agccctttc tcgaccctt 
      ttgtgtctg taaatggtc tattatgac gttcctttg 
      tggaactat tttaaacct cttatttca ctttacatt 
      gaattgtta ccgactcat ttttgtgta gttggtact 
      tttacggta tttaaaaca tcaattttg aattatatc 
      tacataaat cgatgacac caaaaataa cttcaaaaa 
      aatttactt gcagcgtcc agaaattgt ttttttttc 
      gatttgctt tacagagga gttttactc caaatattg 
      gttcgagac ttttccatt ttatattga atttcgtta 
      tatgggaaa ac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY