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Gene information
General information
TC011660 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 16094037..16097020
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011660
    iBeetle screen iB_05071

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 7 (16 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011660 RA
      MQPAILLLC IAVAAALPA QENARNALQ TENDLLDTI 
      YNDCLRKDT TSCLKYKLF SFVDKMLGH KDTITVTEG 
      VQIIKTGSD SDGAPRALS GDETIESLI WNRIQSFLE 
      SHTIKVDLK GSDIVNAVT STARSFNDV LSEEETLEE 
      EGRGKKKKA QKILGPLMA AAALKAAVL GKLALAGIA 
      LIAGKALLI GKIALVLSA IIGLKKLLG QGGKHVTYE 
      VVSHPQHSS SHVASHETV YGGSSGGGG YGGDVGGGY 
      SSSGHGGWG RSLDAQQLA YRGHPQAQ
    CDS sequence
    • >TC011660 RA
      atgcagcca gcaatcctg ttgttgtgt atcgcggtc 
      gctgccgct ttgccggcc caagaaaac gccagaaac 
      gccctccag accgaaaac gacctcttg gacaccatc 
      tacaacgac tgcctccga aaggacacc acgtcatgt 
      ctcaagtac aagctcttc tccttcgtc gacaagatg 
      ttgggacac aaggatacc atcacagtg acggaagga 
      gtgcaaatt atcaaaaca ggaagtgat tcggatgga 
      gctccaaga gctttaagc ggagacgaa accattgaa 
      agtctcatt tggaacagg attcaatct ttcttggaa 
      tcacatacg atcaaggtt gatctcaag ggctccgat 
      attgtgaat gctgtcact tcgactgcc cgatccttc 
      aacgatgtc ctttcggaa gaagagact ttggaagaa 
      gaaggacgt ggaaagaag aagaaagca cagaaaatt 
      ctcggacct ttgatggca gcggccgcc cttaaagct 
      gcagtcttg gggaagttg gctcttgct ggtattgcc 
      ttaatcgct ggtaaagca cttctcatc ggtaaaatc 
      gctttggtg ttgtcggcg attatcgga ttgaagaaa 
      ctacttgga caaggtggc aaacatgtc acttatgaa 
      gtggtttcg caccctcaa cattcttct agtcacgtc 
      gcaagccac gaaaccgtc tatgggggc agtagtgga 
      ggcggcggc tatggcgga gacgttgga ggcggttat 
      tcttcttcg ggacatgga ggttggggc agatctttg 
      gatgcccaa cagctcgcc tacagagga caccctcaa 
      gcccagtaa
    mRNA sequence
    • >TC011660 RA
      acataggat gcaattcca gtttgcaac tcttagttt 
      cgtttatcc cgcggcaaa ttttttgct aggaacagt 
      tatttaact gggaattgc tatcacttt tctctgatt 
      attggttag agttgtgat ctgcgatgc atgtcagta 
      agcgagagt gaagctgtg agaagagag aattgaact 
      agattacgt tggtaggta gcaccttat gtgtgtcgg 
      gtcagcagg tacggtgac ccgatagtg acccaatcc 
      gccccctat ttccggtgt tggacatca ggaacaagc 
      aatgtgtaa ttgtgggtg ctaccaagc gcatagacc 
      ctaggtgca tatcgcaca ggtccaaaa cgaggttag 
      ctacaatga gaaagcggt ccaagatat cgccccgcg 
      gccgtcatt atagtctcc ggacccaaa ttgtgctaa 
      gcttctgag taggaggga taagactca tatatacag 
      gcgtaccat cttcacgac atcacacac cgataacgc 
      atcatgcag ccagcaatc ctgttgttg tgtatcgcg 
      gtcgctgcc gctttgccg gcccaagaa aacgccaga 
      aacgccctc cagaccgaa aacgacctc ttggacacc 
      atctacaac gactgcctc cgaaaggac accacgtca 
      tgtctcaag tacaagctc ttctccttc gtcgacaag 
      atgttggga cacaaggat accatcaca gtgacggaa 
      ggagtgcaa attatcaaa acaggaagt gattcggat 
      ggagctcca agagcttta agcggagac gaaaccatt 
      gaaagtctc atttggaac aggattcaa tctttcttg 
      gaatcacat acgatcaag gttgatctc aagggctcc 
      gatattgtg aatgctgtc acttcgact gcccgatcc 
      ttcaacgat gtcctttcg gaagaagag actttggaa 
      gaagaagga cgtggaaag aagaagaaa gcacagaaa 
      attctcgga cctttgatg gcagcggcc gcccttaaa 
      gctgcagtc ttggggaag ttggctctt gctggtatt 
      gccttaatc gctggtaaa gcacttctc atcggtaaa 
      atcgctttg gtgttgtcg gcgattatc ggattgaag 
      aaactactt ggacaaggt ggcaaacat gtcacttat 
      gaagtggtt tcgcaccct caacattct tctagtcac 
      gtcgcaagc cacgaaacc gtctatggg ggcagtagt 
      ggaggcggc ggctatggc ggagacgtt ggaggcggt 
      tattcttct tcgggacat ggaggttgg ggcagatct 
      ttggatgcc caacagctc gcctacaga ggacaccct 
      caagcccag taaccggcc gagtcggtc ccgtacctg 
      cgaattctc agcgagcat ctagttccg ttcttattt 
      atcttattt atgtattta ttagtctaa cgatgacaa 
      agactaaaa taccatact agatctaga ttaggatta 
      agttttaag tgttatttt tgtcaagtc aataaaatt 
      gtaaacact tt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY