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Gene information
General information
TC011656 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 16072257..16077683
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011656
    iBeetle screen iB_05069

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection



    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011656 RA
      MDEIWRYRT GDMLKVLYV LCLVSTLRA SKLNCESEI 
      DRELKRYVD SVLSIDKYS ILPGIGIER VANLSIKNE 
      KNDETCVSS RSFGSIQEY VKKKLEEYS QTHVLSVDI 
      PVTARFFQT AATTSDDSG GRNSFMAGF GMGFLAFAL 
      KKLLLPVFI GAQLVKSVM IAMFLPSIL GGLGKVLGK 
      GLSTFSGIS GASAGINNN HGQVEDFEF KDTDPYNND 
      GTVQDFNAE GTDNGNLGI TVVGSTTPS TANSRFGIG 
      HNRISFQND NYYMRKPNK KTDYKVFHK IPSSSLLLT 
      SYDPFYSPL LSRLDAVFQ QLGLGDDKS PEIEKCRER 
      LVCMMYANP AKYAPYSNL VSAQLSREL NELRKPASD 
      NPDILRFFR YMKAAKDGQ DGEECLAHK GCSTLSTSQ 
      ASPAILTTY NEINKLVQA RKIN
    CDS sequence
    • >TC011656 RA
      atggacgag atatggcgt tacagaaca ggtgacatg 
      ctaaaagtt ctgtacgta ttgtgtttg gtgtcgact 
      ttgagggct tcaaaactg aactgtgaa agtgagatt 
      gatagggaa ttaaaacga tatgttgat agtgtcttg 
      agtatcgat aaatattcc attttgccg ggaattgga 
      atcgaacga gtggctaat cttagcatc aaaaatgag 
      aaaaacgat gaaacgtgc gtgagttca aggagtttt 
      ggaagtatt caagagtac gtcaaaaaa aagttggag 
      gagtacagc cagactcat gtgctttcg gtggacatt 
      ccggtcacg gcccggttt ttccaaact gcggctaca 
      acatcagat gacagcggc ggcagaaat tcgttcatg 
      gccggtttc ggtatgggc tttttagct tttgccttg 
      aaaaaactc cttttgccg gtttttatc ggggctcag 
      ttggtgaaa tcggtgatg atcgcaatg ttcctgcca 
      tcaattctc ggaggttta ggaaaggta cttggaaag 
      ggattatcg acgttttcg ggaatctct ggggcgtca 
      gcgggaatc aacaacaac cacggacaa gttgaagat 
      ttcgaattt aaagatact gatccttac aacaatgac 
      ggaacagtc caggatttc aacgctgag ggaaccgat 
      aatggcaac ttgggaatc actgtggtg ggctccaca 
      acgccgtca acagctaac agcaggttt ggaatcggc 
      cacaaccgg atttccttc caaaacgac aactactac 
      atgcgaaag cccaacaag aagactgac tataaggtg 
      ttccacaaa attccgtct tcttcgctt cttctcaca 
      agttatgat cctttctac agccctctt ctttcgcgt 
      ctcgatgcc gttttccag caattaggc ctcggtgac 
      gataaatca ccggaaatc gaaaaatgt cgcgagaga 
      cttgtctgc atgatgtac gcgaatcca gcgaaatac 
      gccccatat agtaacctc gtttcagca cagctgagc 
      agggagcta aacgagttg cggaagccg gcttcggac 
      aatccggat attctcagg ttcttccgg tatatgaaa 
      gcggcaaaa gatggccaa gatggagag gaatgtctg 
      gcacataaa gggtgctcg accttatct accagtcaa 
      gctagtcca gctattctt actacgtac aatgaaatt 
      aacaaattg gtgcaagcc agaaagatc aattga
    mRNA sequence
    • >TC011656 RA
      acgtattca ttatttctt caacacaaa gaatttgtg 
      aacacgtgc tctgggtgt tcagagttc ccacacgtt 
      cgtgccaaa atggataga agatactga ctgacaaca 
      gatggcctc aaaatggac gagatatgg cgttacaga 
      acaggtgac atgctaaaa gttctgtac gtattgtgt 
      ttggtgtcg actttgagg gcttcaaaa ctgaactgt 
      gaaagtgag attgatagg gaattaaaa cgatatgtt 
      gatagtgtc ttgagtatc gataaatat tccattttg 
      ccgggaatt ggaatcgaa cgagtggct aatcttagc 
      atcaaaaat gagaaaaac gatgaaacg tgcgtgagt 
      tcaaggagt tttggaagt attcaagag tacgtcaaa 
      aaaaagttg gaggagtac agccagact catgtgctt 
      tcggtggac attccggtc acggcccgg tttttccaa 
      actgcggct acaacatca gatgacagc ggcggcaga 
      aattcgttc atggccggt ttcggtatg ggcttttta 
      gcttttgcc ttgaaaaaa ctccttttg ccggttttt 
      atcggggct cagttggtg aaatcggtg atgatcgca 
      atgttcctg ccatcaatt ctcggaggt ttaggaaag 
      gtacttgga aagggatta tcgacgttt tcgggaatc 
      tctggggcg tcagcggga atcaacaac aaccacgga 
      caagttgaa gatttcgaa tttaaagat actgatcct 
      tacaacaat gacggaaca gtccaggat ttcaacgct 
      gagggaacc gataatggc aacttggga atcactgtg 
      gtgggctcc acaacgccg tcaacagct aacagcagg 
      tttggaatc ggccacaac cggatttcc ttccaaaac 
      gacaactac tacatgcga aagcccaac aagaagact 
      gactataag gtgttccac aaaattccg tcttcttcg 
      cttcttctc acaagttat gatcctttc tacagccct 
      cttctttcg cgtctcgat gccgttttc cagcaatta 
      ggcctcggt gacgataaa tcaccggaa atcgaaaaa 
      tgtcgcgag agacttgtc tgcatgatg tacgcgaat 
      ccagcgaaa tacgcccca tatagtaac ctcgtttca 
      gcacagctg agcagggag ctaaacgag ttgcggaag 
      ccggcttcg gacaatccg gatattctc aggttcttc 
      cggtatatg aaagcggca aaagatggc caagatgga 
      gaggaatgt ctggcacat aaagggtgc tcgacctta 
      tctaccagt caagctagt ccagctatt cttactacg 
      tacaatgaa attaacaaa ttggtgcaa gccagaaag 
      atcaattga gtgattttg tatcttagt catattgat 
      attttaagc ctaatttat ttatttatt taatttatt 
      gacattttt tctgttgta gttgctcaa tccgtacac 
      tatctattt atttactta tttatttta tttatttat 
      ttatgtgtc tgttgtaaa ttatttttc cgaaaaaat 
      ttttacata taataaata gtttctgaa ttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY