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Gene information
General information
TC011656 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 16072257..16077683
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC011656
    iBeetle screen iB_05069

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011656 RA
      MDEIWRYRT GDMLKVLYV LCLVSTLRA SKLNCESEI 
DRELKRYVD SVLSIDKYS ILPGIGIER VANLSIKNE 
KNDETCVSS RSFGSIQEY VKKKLEEYS QTHVLSVDI 
PVTARFFQT AATTSDDSG GRNSFMAGF GMGFLAFAL 
KKLLLPVFI GAQLVKSVM IAMFLPSIL GGLGKVLGK 
GLSTFSGIS GASAGINNN HGQVEDFEF KDTDPYNND 
GTVQDFNAE GTDNGNLGI TVVGSTTPS TANSRFGIG 
HNRISFQND NYYMRKPNK KTDYKVFHK IPSSSLLLT 
SYDPFYSPL LSRLDAVFQ QLGLGDDKS PEIEKCRER 
LVCMMYANP AKYAPYSNL VSAQLSREL NELRKPASD 
NPDILRFFR YMKAAKDGQ DGEECLAHK GCSTLSTSQ 
ASPAILTTY NEINKLVQA RKIN
    CDS sequence
    • >TC011656 RA
      atggacgag atatggcgt tacagaaca ggtgacatg 
ctaaaagtt ctgtacgta ttgtgtttg gtgtcgact 
ttgagggct tcaaaactg aactgtgaa agtgagatt 
gatagggaa ttaaaacga tatgttgat agtgtcttg 
agtatcgat aaatattcc attttgccg ggaattgga 
atcgaacga gtggctaat cttagcatc aaaaatgag 
aaaaacgat gaaacgtgc gtgagttca aggagtttt 
ggaagtatt caagagtac gtcaaaaaa aagttggag 
gagtacagc cagactcat gtgctttcg gtggacatt 
ccggtcacg gcccggttt ttccaaact gcggctaca 
acatcagat gacagcggc ggcagaaat tcgttcatg 
gccggtttc ggtatgggc tttttagct tttgccttg 
aaaaaactc cttttgccg gtttttatc ggggctcag 
ttggtgaaa tcggtgatg atcgcaatg ttcctgcca 
tcaattctc ggaggttta ggaaaggta cttggaaag 
ggattatcg acgttttcg ggaatctct ggggcgtca 
gcgggaatc aacaacaac cacggacaa gttgaagat 
ttcgaattt aaagatact gatccttac aacaatgac 
ggaacagtc caggatttc aacgctgag ggaaccgat 
aatggcaac ttgggaatc actgtggtg ggctccaca 
acgccgtca acagctaac agcaggttt ggaatcggc 
cacaaccgg atttccttc caaaacgac aactactac 
atgcgaaag cccaacaag aagactgac tataaggtg 
ttccacaaa attccgtct tcttcgctt cttctcaca 
agttatgat cctttctac agccctctt ctttcgcgt 
ctcgatgcc gttttccag caattaggc ctcggtgac 
gataaatca ccggaaatc gaaaaatgt cgcgagaga 
cttgtctgc atgatgtac gcgaatcca gcgaaatac 
gccccatat agtaacctc gtttcagca cagctgagc 
agggagcta aacgagttg cggaagccg gcttcggac 
aatccggat attctcagg ttcttccgg tatatgaaa 
gcggcaaaa gatggccaa gatggagag gaatgtctg 
gcacataaa gggtgctcg accttatct accagtcaa 
gctagtcca gctattctt actacgtac aatgaaatt 
aacaaattg gtgcaagcc agaaagatc aattga
    mRNA sequence
    • >TC011656 RA
      acgtattca ttatttctt caacacaaa gaatttgtg 
aacacgtgc tctgggtgt tcagagttc ccacacgtt 
cgtgccaaa atggataga agatactga ctgacaaca 
gatggcctc aaaatggac gagatatgg cgttacaga 
acaggtgac atgctaaaa gttctgtac gtattgtgt 
ttggtgtcg actttgagg gcttcaaaa ctgaactgt 
gaaagtgag attgatagg gaattaaaa cgatatgtt 
gatagtgtc ttgagtatc gataaatat tccattttg 
ccgggaatt ggaatcgaa cgagtggct aatcttagc 
atcaaaaat gagaaaaac gatgaaacg tgcgtgagt 
tcaaggagt tttggaagt attcaagag tacgtcaaa 
aaaaagttg gaggagtac agccagact catgtgctt 
tcggtggac attccggtc acggcccgg tttttccaa 
actgcggct acaacatca gatgacagc ggcggcaga 
aattcgttc atggccggt ttcggtatg ggcttttta 
gcttttgcc ttgaaaaaa ctccttttg ccggttttt 
atcggggct cagttggtg aaatcggtg atgatcgca 
atgttcctg ccatcaatt ctcggaggt ttaggaaag 
gtacttgga aagggatta tcgacgttt tcgggaatc 
tctggggcg tcagcggga atcaacaac aaccacgga 
caagttgaa gatttcgaa tttaaagat actgatcct 
tacaacaat gacggaaca gtccaggat ttcaacgct 
gagggaacc gataatggc aacttggga atcactgtg 
gtgggctcc acaacgccg tcaacagct aacagcagg 
tttggaatc ggccacaac cggatttcc ttccaaaac 
gacaactac tacatgcga aagcccaac aagaagact 
gactataag gtgttccac aaaattccg tcttcttcg 
cttcttctc acaagttat gatcctttc tacagccct 
cttctttcg cgtctcgat gccgttttc cagcaatta 
ggcctcggt gacgataaa tcaccggaa atcgaaaaa 
tgtcgcgag agacttgtc tgcatgatg tacgcgaat 
ccagcgaaa tacgcccca tatagtaac ctcgtttca 
gcacagctg agcagggag ctaaacgag ttgcggaag 
ccggcttcg gacaatccg gatattctc aggttcttc 
cggtatatg aaagcggca aaagatggc caagatgga 
gaggaatgt ctggcacat aaagggtgc tcgacctta 
tctaccagt caagctagt ccagctatt cttactacg 
tacaatgaa attaacaaa ttggtgcaa gccagaaag 
atcaattga gtgattttg tatcttagt catattgat 
attttaagc ctaatttat ttatttatt taatttatt 
gacattttt tctgttgta gttgctcaa tccgtacac 
tatctattt atttactta tttatttta tttatttat 
ttatgtgtc tgttgtaaa ttatttttc cgaaaaaat 
ttttacata taataaata gtttctgaa ttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY