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Gene information
General information
TC011626 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15660283..15662059
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011626
    iBeetle screen iB_05064

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    legs number increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011626 RA
      MADAKKVLE YKDSLKKEA EELLLKGFP ATIVKLNAL 
      LETPQFKNR KFTDVHQEL NIPVPDPIL LNSHTDLPP 
      AKKAKYDNS ISQDGTKVM ILPTGAVPT NKHLVELVE 
      AVKPHIRKL VEDSNTMKM WISFMIPKI EDGNNFGVS 
      IQEDTLAEV QSVESEAAA FFDQISRYF ISRAKVISK 
      VAKYPHIED YRRAVQELD EKEYLSLWL VMCEIRNRY 
      CALHDIVIK NLDKIKKPR STNATESLY
    CDS sequence
    • >TC011626 RA
      atggcagat gcaaaaaag gtgttggaa tacaaagac 
      agtctgaag aaagaggcc gaagagttg ctgcttaag 
      gggttcccc gcgacgatt gtgaagctc aacgctttg 
      ctagaaacg ccccagttc aaaaatcgg aaattcact 
      gacgtgcac caggagctg aacatcccg gtgccagac 
      cccattttg ctcaacagc cacacggac ctgcctcca 
      gccaagaag gccaagtat gacaatagc atatcgcaa 
      gacggcacc aaggtcatg atcctgccc acgggggct 
      gtgcccact aacaagcat ctggtcgag ttggtcgag 
      gctgtgaag ccccacatc cgcaaactg gttgaagac 
      tccaatact atgaaaatg tggatttct tttatgatt 
      ccgaaaatc gaagacggc aacaacttc ggggtgtcg 
      atccaggag gacactctg gccgaggtg cagtcggtg 
      gaatcggag gcggcggcg tttttcgat caaatctcg 
      cgatatttc atttctcga gccaaggtc atctcgaaa 
      gtggcgaaa tacccgcac atagaggac taccgaagg 
      gccgtgcag gagctggat gagaaggag tacttgagt 
      ttgtggttg gtcatgtgc gagatccga aatcggtat 
      tgtgccctt cacgacata gttattaaa aacctcgac 
      aaaatcaag aagccacgt tccactaat gctactgaa 
      tctttgtac taa
    mRNA sequence
    • >TC011626 RA
      ggccaattt ggtcgctag ttttattgc caacctaaa 
      tcgcaaatc gcgaaatca cgctgtaaa tatttgttg 
      tatagaaat tccagatca gtgggatca aattttagc 
      aatctggca caagttgtt gcatgattc aattagttg 
      ttgatttgg tggattggg gatcgcgtc ctggtgcta 
      ggcccaatt ctcgtcctt tcttttgcc attgcccca 
      aacatttag gttaagtca actcaagtg aacagttca 
      aaagttagt tctaattgt atattatgg cagatgcaa 
      aaaaggtgt tggaataca aagacagtc tgaagaaag 
      aggccgaag agttgctgc ttaaggggt tccccgcga 
      cgattgtga agctcaacg ctttgctag aaacgcccc 
      agttcaaaa atcggaaat tcactgacg tgcaccagg 
      agctgaaca tcccggtgc cagacccca ttttgctca 
      acagccaca cggacctgc ctccagcca agaaggcca 
      agtatgaca atagcatat cgcaagacg gcaccaagg 
      tcatgatcc tgcccacgg gggctgtgc ccactaaca 
      agcatctgg tcgagttgg tcgaggctg tgaagcccc 
      acatccgca aactggttg aagactcca atactatga 
      aaatgtgga tttctttta tgattccga aaatcgaag 
      acggcaaca acttcgggg tgtcgatcc aggaggaca 
      ctctggccg aggtgcagt cggtggaat cggaggcgg 
      cggcgtttt tcgatcaaa tctcgcgat atttcattt 
      ctcgagcca aggtcatct cgaaagtgg cgaaatacc 
      cgcacatag aggactacc gaagggccg tgcaggagc 
      tggatgaga aggagtact tgagtttgt ggttggtca 
      tgtgcgaga tccgaaatc ggtattgtg cccttcacg 
      acatagtta ttaaaaacc tcgacaaaa tcaagaagc 
      cacgttcca ctaatgcta ctgaatctt tgtactaaa 
      acagataaa taaatgcat ctttcgaat tttgatttt 
      tgtaaagtg tgtacgtga caatgagta ttatacaat 
      attttcgta ataaagtta ttgtgagtt tttttatct 
      gtaagggaa cttgtagtg acacttagc agtgagtag 
      ctgttgggg ttatttttt atttaaaat aatacattt 
      cataaataa attataaaa cgactt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY