Status
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Gene information
General information
TC011615 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15469603..15471721
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC011615
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
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      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
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      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_05063

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

      pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC011615 RA
        MRPNIRVEI QNNLVEGME VDDNEEGEI SDSDSKGAL 
        AQQTGPLQP NERLLEKSG LLDKKEKLQ ERARRFCLN 
        PEEIKNFTD DDLHLLHSS LGINATNES NVRFEVIHL 
        RGIGQMTAG DILDYFLEY APVSVEWID EECCNVVWG 
        DKIAAARAL HFMSKPVRG MPVRDDTSY FDNLNESED 
        DQSILILNK NREIQLQEE IEENPLSKL LNGANSVDI 
        SEITSTIPP GYWRLGKKH PNFKCILMR FALISDKKP 
        FKSEQFARY YKNQGKKPL KGGTSGIFG RNKDMKTCD 
        ENPWSVLAK NWDEDSAFR EKERTEPKV EVRNESLRV 
        RLGTKRVKD EDGGEPVKK TKIPRMRMY ADEEEEKMK 
        RKQMLKRIN TQIRKIDED KSSNSDLRA VLNMANRTH 
        AKLDKTEPE VFDLGTRLK NRSKKMVFE IKTEPAVKP 
        RRRRQSPQS EEEEEEFDD YRHDKPKSK VAVVIKTQK 
        KPAVASAVA WSRVSGSEE EEESENERE TVYKSPLKI 
        EINNDHFKR K
      CDS sequence
      • >TC011615 RA
        atgcggcca aacattcgc gtggaaatc caaaataac 
        ctcgtcgag ggcatggaa gtggacgac aacgaggag 
        ggcgagatc tccgacagc gactccaag ggggcccta 
        gcccaacaa acgggcccg ctgcagcca aacgaacgg 
        ctcctggag aagtcgggc cttctggac aagaaggag 
        aagctgcaa gagcgcgct cgtcgcttc tgcttgaac 
        ccggaagag ataaagaat ttcaccgac gacgacttg 
        cacttgctt cactctagt ttgggcata aatgccaca 
        aatgagtca aacgtgagg tttgaggta atccatttg 
        cgaggcatc ggccaaatg accgcgggc gatatttta 
        gattatttt ctggaatat gccccagtt tccgtcgaa 
        tggatagac gaggagtgc tgtaacgtc gtctggggc 
        gataaaatc gccgccgca agggcactg catttcatg 
        tccaagccg gtcagaggc atgcccgtg cgggacgac 
        accagttat ttcgacaac ttgaacgag agtgaagac 
        gaccaaagc atcctcatt ttgaacaaa aaccgcgaa 
        atacagcta caagaagaa atcgaggaa aatcccttg 
        agcaagttg ctgaacggc gcgaattca gtcgacatt 
        tccgaaatt acctcgacc atacctccg ggttactgg 
        cgtttgggc aaaaaacac cccaacttc aagtgcatc 
        ctaatgagg ttcgcccta atcagcgac aaaaaaccc 
        ttcaagtcg gaacaattc gctcgttac tacaaaaac 
        caggggaaa aagccccta aaaggcggc accagtggc 
        attttcggc cgcaacaaa gacatgaaa acctgcgac 
        gaaaacccc tggtcagtc ctcgccaaa aactgggac 
        gaggactcg gcatttcgc gaaaaagag cgaaccgaa 
        ccgaaagtc gaagttaga aacgaaagt ctccgagtg 
        cgcctcggc accaaacgg gtaaaagac gaagatggc 
        ggcgaaccg gtgaagaag acgaagatt ccacgcatg 
        cgcatgtac gccgacgag gaggaagag aagatgaag 
        cggaagcaa atgctgaaa cggatcaat acacagatt 
        cggaagatt gacgaagat aaatcgtcg aattccgac 
        ttgagggcg gttttgaat atggcgaac cggacgcac 
        gccaagttg gataaaacc gaaccggaa gtgttcgat 
        ttggggacg cggttgaag aaccggagc aagaaaatg 
        gtttttgag attaaaacc gaaccggcg gttaaaccg 
        cgacggagg agacagtcg ccgcaaagc gaggaggag 
        gaggaggag tttgatgat tataggcat gataaaccg 
        aaaagtaag gtggctgtg gttattaaa acgcagaag 
        aaaccggct gttgcctcg gctgtcgcc tggtcaagg 
        gttagtggg agtgaggag gaggaggag tccgaaaat 
        gagcgggag actgtctac aagagtccg ttgaagatt 
        gaaatcaat aacgaccac tttaagagg aaataa
      mRNA sequence
      • >TC011615 RA
        taactttca acgataacc gctagaata atgtaaaag 
        ttttctcgg tatcgtgtt tatttggta gtattttat 
        cacaaaacg tccgaaaat gcggccaaa cattcgcgt 
        ggaaatcca aaataacct cgtcgaggg catggaagt 
        ggacgacaa cgaggaggg cgagatctc cgacagcga 
        ctccaaggg ggccctagc ccaacaaac gggcccgct 
        gcagccaaa cgaacggct cctggagaa gtcgggcct 
        tctggacaa gaaggagaa gctgcaaga gcgcgctcg 
        tcgcttctg cttgaaccc ggaagagat aaagaattt 
        caccgacga cgacttgca cttgcttca ctctagttt 
        gggcataaa tgccacaaa tgagtcaaa cgtgaggtt 
        tgaggtaat ccatttgcg aggcatcgg ccaaatgac 
        cgcgggcga tattttaga ttattttct ggaatatgc 
        cccagtttc cgtcgaatg gatagacga ggagtgctg 
        taacgtcgt ctggggcga taaaatcgc cgccgcaag 
        ggcactgca tttcatgtc caagccggt cagaggcat 
        gcccgtgcg ggacgacac cagttattt cgacaactt 
        gaacgagag tgaagacga ccaaagcat cctcatttt 
        gaacaaaaa ccgcgaaat acagctaca agaagaaat 
        cgaggaaaa tcccttgag caagttgct gaacggcgc 
        gaattcagt cgacatttc cgaaattac ctcgaccat 
        acctccggg ttactggcg tttgggcaa aaaacaccc 
        caacttcaa gtgcatcct aatgaggtt cgccctaat 
        cagcgacaa aaaaccctt caagtcgga acaattcgc 
        tcgttacta caaaaacca ggggaaaaa gcccctaaa 
        aggcggcac cagtggcat tttcggccg caacaaaga 
        catgaaaac ctgcgacga aaacccctg gtcagtcct 
        cgccaaaaa ctgggacga ggactcggc atttcgcga 
        aaaagagcg aaccgaacc gaaagtcga agttagaaa 
        cgaaagtct ccgagtgcg cctcggcac caaacgggt 
        aaaagacga agatggcgg cgaaccggt gaagaagac 
        gaagattcc acgcatgcg catgtacgc cgacgagga 
        ggaagagaa gatgaagcg gaagcaaat gctgaaacg 
        gatcaatac acagattcg gaagattga cgaagataa 
        atcgtcgaa ttccgactt gagggcggt tttgaatat 
        ggcgaaccg gacgcacgc caagttgga taaaaccga 
        accggaagt gttcgattt ggggacgcg gttgaagaa 
        ccggagcaa gaaaatggt ttttgagat taaaaccga 
        accggcggt taaaccgcg acggaggag acagtcgcc 
        gcaaagcga ggaggagga ggaggagtt tgatgatta 
        taggcatga taaaccgaa aagtaaggt ggctgtggt 
        tattaaaac gcagaagaa accggctgt tgcctcggc 
        tgtcgcctg gtcaagggt tagtgggag tgaggagga 
        ggaggagtc cgaaaatga gcgggagac tgtctacaa 
        gagtccgtt gaagattga aatcaataa cgaccactt 
        taagaggaa ataatttac ttttttaat ttattttat 
        ttactgtaa ttgtgaggt tttttaaat tcttgtatg 
        tatataagt gaaggattt cgatattaa aactacaaa 
        aacgca
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY