Status
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Gene information
General information
TC011606 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15268092..15269793
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011606
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05060

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011606 RA
      MSGGSQQVI NFTLYMLCF EILSTNSRT STLWKLNSD 
      QTKIIEANS FHQVVSYPE GSTGVQFIT EDDPIFNII 
      TSTVHLGHS WIKERVEHY CTNCHVKNL KLVNSTNMV 
      VKNSVTKSE PDEVLDCGK PVNFTYYDN LVGVLNRNK 
      HPIVPEPQA ALIFTKKTK KKLQDFDVD ALERKLKKA 
      KREKPKSVQ LYNQIGNFW RIKGDAEKA IECFRRALA 
      VSPHNAEVL LNLARVLFT LQYLDDAIY LTRRSLEVQ 
      PPDKGAWQQ YFTLGEILK AYGHYQEAS IHLKHTLEL 
      KPGFEPAQN ALKEMETMP TASIHVYTL VIIICLVLG 
      VLLVILSSV DNMGEGEYA EVKTQRHFN KAMAMRSLK 
      GFGGSQKRA KRILA
    • >TC011606 RB
      MSGGSQQVI NFTLYMLCF EILSTNSRT STLWKLNSD 
      QTKIIEANS FHQVVSYPE GSTGVQFIT EDDPIFNII 
      TSTVHLGHS WIKERVEHY CTNCHVKNL NTNMVVKNS 
      VTKSEPDEV LDCGKPVNF TYYDNLVGV LNRNKHPIV 
      PEPQAALIF TKKTKKKLQ DFDVDALER KLKKAKREK 
      PKSVQLYNQ IGNFWRIKG DAEKAIECF RRALAVSPH 
      NAEVLLNLA RVLFTLQYL DDAIYLTRR SLEVQPPDK 
      GAWQQYFTL GEILKAYGH YQEASIHLK HTLELKPGF 
      EPAQNALKE METMPTASI HVYTLVIII CLVLGVLLV 
      ILSSVDNMG EGEYAEVKT QRHFNKAMA MRSLKGFGG 
      SQKRAKRIL A
    CDS sequence
    • >TC011606 RA
      atgagtggt ggttcgcag caagtgatt aattttacg 
      ctttatatg ttatgtttt gagatactt tcgactaat 
      tcccgcact tcgacactg tggaaactc aattcggat 
      caaacgaaa atcattgag gctaattcc ttccaccag 
      gttgtctcg tatcccgag ggctcgact ggggtgcag 
      ttcatcaca gaggatgac cccattttc aacataatc 
      acgtccacg gtgcatttg gggcactcg tggattaag 
      gaacgagtc gagcattac tgtaccaac tgtcacgtt 
      aaaaattta aaacttgta aatagcaca aacatggtt 
      gtgaaaaac tctgtgact aaatccgaa ccggacgaa 
      gtcctcgat tgcggaaaa cccgtcaat ttcacctac 
      tacgacaac ttagttggc gttctaaac cgaaacaag 
      caccccatt gtcccggag ccccaagcg gctctgatt 
      ttcaccaaa aaaacgaaa aaaaagcta caagacttc 
      gacgtggac gctttggag cgcaaactc aagaaagcg 
      aagcgcgag aagcccaaa tcggtgcag ttgtacaac 
      caaattggc aatttttgg cgcattaaa ggcgacgcc 
      gaaaaagcc atcgagtgt tttcggcgc gctcttgcc 
      gtttcgccg cacaatgcc gaggttttg ctcaatttg 
      gcgcgcgtt ttgttcact ttgcaatac ttggacgat 
      gcgatttac ttgacgagg aggtcgctc gaggtgcag 
      cccccggat aagggcgcc tggcagcag tattttacg 
      ctgggggag attcttaag gcttatgga cactatcag 
      gaggcttcg attcatttg aaacacacg ctggagcta 
      aagccgggc tttgaaccg gcgcaaaat gcacttaag 
      gagatggag acgatgccg actgccagc atacatgtc 
      tacacactt gttataatt atttgcctg gttttaggt 
      gttttgttg gtgatttta tcgtctgtg gataatatg 
      ggcgagggt gagtatgca gaagtgaag acgcaacgg 
      cattttaac aaggcaatg gcgatgagg agtttgaag 
      gggtttggg ggatcacag aagcgcgca aagaggatt 
      cttgcctag
    • >TC011606 RB
      atgagtggt ggttcgcag caagtgatt aattttacg 
      ctttatatg ttatgtttt gagatactt tcgactaat 
      tcccgcact tcgacactg tggaaactc aattcggat 
      caaacgaaa atcattgag gctaattcc ttccaccag 
      gttgtctcg tatcccgag ggctcgact ggggtgcag 
      ttcatcaca gaggatgac cccattttc aacataatc 
      acgtccacg gtgcatttg gggcactcg tggattaag 
      gaacgagtc gagcattac tgtaccaac tgtcacgtt 
      aaaaattta aacacaaac atggttgtg aaaaactct 
      gtgactaaa tccgaaccg gacgaagtc ctcgattgc 
      ggaaaaccc gtcaatttc acctactac gacaactta 
      gttggcgtt ctaaaccga aacaagcac cccattgtc 
      ccggagccc caagcggct ctgattttc accaaaaaa 
      acgaaaaaa aagctacaa gacttcgac gtggacgct 
      ttggagcgc aaactcaag aaagcgaag cgcgagaag 
      cccaaatcg gtgcagttg tacaaccaa attggcaat 
      ttttggcgc attaaaggc gacgccgaa aaagccatc 
      gagtgtttt cggcgcgct cttgccgtt tcgccgcac 
      aatgccgag gttttgctc aatttggcg cgcgttttg 
      ttcactttg caatacttg gacgatgcg atttacttg 
      acgaggagg tcgctcgag gtgcagccc ccggataag 
      ggcgcctgg cagcagtat tttacgctg ggggagatt 
      cttaaggct tatggacac tatcaggag gcttcgatt 
      catttgaaa cacacgctg gagctaaag ccgggcttt 
      gaaccggcg caaaatgca cttaaggag atggagacg 
      atgccgact gccagcata catgtctac acacttgtt 
      ataattatt tgcctggtt ttaggtgtt ttgttggtg 
      attttatcg tctgtggat aatatgggc gagggtgag 
      tatgcagaa gtgaagacg caacggcat tttaacaag 
      gcaatggcg atgaggagt ttgaagggg tttggggga 
      tcacagaag cgcgcaaag aggattctt gcctag
    mRNA sequence
    • >TC011606 RA
      ccccaaaaa tcaaaaaaa gctcatttc caagcaatg 
      gcattaaaa agcgacttt gccccgatt cacactgag 
      cgtggagct attaaaccg taaatgagc aaacttgtt 
      taagtttgt cacaagtcc tgaaacgtt ctagtcaat 
      aatgagtgg tggttcgca gcaagtgat taattttac 
      gctttatat gttatgttt tgagatact ttcgactaa 
      ttcccgcac ttcgacact gtggaaact caattcgga 
      tcaaacgaa aatcattga ggctaattc cttccacca 
      ggttgtctc gtatcccga gggctcgac tggggtgca 
      gttcatcac agaggatga ccccatttt caacataat 
      cacgtccac ggtgcattt ggggcactc gtggattaa 
      ggaacgagt cgagcatta ctgtaccaa ctgtcacgt 
      taaaaattt aaaacttgt aaatagcac aaacatggt 
      tgtgaaaaa ctctgtgac taaatccga accggacga 
      agtcctcga ttgcggaaa acccgtcaa tttcaccta 
      ctacgacaa cttagttgg cgttctaaa ccgaaacaa 
      gcaccccat tgtcccgga gccccaagc ggctctgat 
      tttcaccaa aaaaacgaa aaaaaagct acaagactt 
      cgacgtgga cgctttgga gcgcaaact caagaaagc 
      gaagcgcga gaagcccaa atcggtgca gttgtacaa 
      ccaaattgg caatttttg gcgcattaa aggcgacgc 
      cgaaaaagc catcgagtg ttttcggcg cgctcttgc 
      cgtttcgcc gcacaatgc cgaggtttt gctcaattt 
      ggcgcgcgt tttgttcac tttgcaata cttggacga 
      tgcgattta cttgacgag gaggtcgct cgaggtgca 
      gcccccgga taagggcgc ctggcagca gtattttac 
      gctggggga gattcttaa ggcttatgg acactatca 
      ggaggcttc gattcattt gaaacacac gctggagct 
      aaagccggg ctttgaacc ggcgcaaaa tgcacttaa 
      ggagatgga gacgatgcc gactgccag catacatgt 
      ctacacact tgttataat tatttgcct ggttttagg 
      tgttttgtt ggtgatttt atcgtctgt ggataatat 
      gggcgaggg tgagtatgc agaagtgaa gacgcaacg 
      gcattttaa caaggcaat ggcgatgag gagtttgaa 
      ggggtttgg gggatcaca gaagcgcgc aaagaggat 
      tcttgccta gtgttgtac ctggattgt gattaataa 
      attgtctga aagttt
    • >TC011606 RB
      ccccaaaaa tcaaaaaaa gctcatttc caagcaatg 
      gcattaaaa agcgacttt gccccgatt cacactgag 
      cgtggagct attaaaccg taaatgagc aaacttgtt 
      taagtttgt cacaagtcc tgaaacgtt ctagtcaat 
      aatgagtgg tggttcgca gcaagtgat taattttac 
      gctttatat gttatgttt tgagatact ttcgactaa 
      ttcccgcac ttcgacact gtggaaact caattcgga 
      tcaaacgaa aatcattga ggctaattc cttccacca 
      ggttgtctc gtatcccga gggctcgac tggggtgca 
      gttcatcac agaggatga ccccatttt caacataat 
      cacgtccac ggtgcattt ggggcactc gtggattaa 
      ggaacgagt cgagcatta ctgtaccaa ctgtcacgt 
      taaaaattt aaacacaaa catggttgt gaaaaactc 
      tgtgactaa atccgaacc ggacgaagt cctcgattg 
      cggaaaacc cgtcaattt cacctacta cgacaactt 
      agttggcgt tctaaaccg aaacaagca ccccattgt 
      cccggagcc ccaagcggc tctgatttt caccaaaaa 
      aacgaaaaa aaagctaca agacttcga cgtggacgc 
      tttggagcg caaactcaa gaaagcgaa gcgcgagaa 
      gcccaaatc ggtgcagtt gtacaacca aattggcaa 
      tttttggcg cattaaagg cgacgccga aaaagccat 
      cgagtgttt tcggcgcgc tcttgccgt ttcgccgca 
      caatgccga ggttttgct caatttggc gcgcgtttt 
      gttcacttt gcaatactt ggacgatgc gatttactt 
      gacgaggag gtcgctcga ggtgcagcc cccggataa 
      gggcgcctg gcagcagta ttttacgct gggggagat 
      tcttaaggc ttatggaca ctatcagga ggcttcgat 
      tcatttgaa acacacgct ggagctaaa gccgggctt 
      tgaaccggc gcaaaatgc acttaagga gatggagac 
      gatgccgac tgccagcat acatgtcta cacacttgt 
      tataattat ttgcctggt tttaggtgt tttgttggt 
      gattttatc gtctgtgga taatatggg cgagggtga 
      gtatgcaga agtgaagac gcaacggca ttttaacaa 
      ggcaatggc gatgaggag tttgaaggg gtttggggg 
      atcacagaa gcgcgcaaa gaggattct tgcctagtg 
      ttgtacctg gattgtgat taataaatt gtctgaaag 
      ttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY