Status
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Gene information
General information
TC011603 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15226203..15235715
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011603
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05059

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011603 RA
      MPREWSGLP VAVLCAFGV IFADAACWF NSELHDEGV 
      EVSTTEPCL NCTCSRGTL LCYLRVCPQ LPNPPPAGC 
      ILLHRYHTC CPELICTDS FEGSNGLEA RSEPNEEFD 
      LGDPKALVG NACVVNGSV YGPGSAMDS STFCEYCYC 
      LRGKQICVK PKCLLPVDG CVPMYEETN CCPVHYNCT 
      YDTPSTSTT TVQTTQLEL NQGGCMVDG VYYKEGGKV 
      LGIGYSVCD NCYCLRGIL RCEPLSCAP PLFGCTPVI 
      RPGECCAAS YNCSGTIEI QPEPNYGHY PIISKDYAK 
      FRKEVHDKN SVTVPTIKN QRTHGSTRH FTGPAFSSS 
      TVKPFFYNS ITTQGSKIL PKVDQNSLD DRLNRRVET 
      IETTEMTST VDTTTMTEV FSTETTETT ESESTTEFT 
      TTESFPITL KTVLNSTDC TNLNEKSDS FELVETTTL 
      PTTTSVDLE KQETVIFTT KIHTQNTTP ALDLALIYN 
      ITKSKDPDY EYDYSEPSL PPSLPNLRI IPFVAADAL 
      DLENEKHNV IHPQERVTD ISHTYNLFS PPVETEGGF 
      IPKQPPILD NLYDEIVTT TTTISPQLR EITCILDEQ 
      EINHGESIV SETTCNTCG CFYGNIVCQ KTICPIPKI 
      DCQKFLLQD PTTCCPLFA CDNELPTVV LDRMDSPET 
      VTPDPFRDV IRTEPAPNL QSLIVDVLK RKTTTEKPF 
      SLDKVLQLL FSTEEEVTT TTSTTTTST TTQRLSTTE 
      FDSNTVDSS TSNVGILKL AGCNIYGRM YRVGRIISE 
      LSNPCLECR CTETGVQCR PLKC
    CDS sequence
    • >TC011603 RA
      atgcctcgc gagtggagt ggccttcct gtggccgtg 
      ttgtgtgcc tttggagtc atttttgct gatgcagcc 
      tgctggttc aactcggaa ctccacgac gaaggcgtc 
      gaggtgtcc accaccgag ccttgcctc aactgcacc 
      tgcagccgg ggcacgctt ttgtgctac ttgcgcgtc 
      tgcccccag ctgcccaac ccgccgccc gccggctgc 
      atcctcctc catcgctac cacacctgc tgccctgaa 
      ctcatctgc acagacagt tttgaaggc agcaacggc 
      ttggaggcg cgctccgaa ccgaacgag gaattcgac 
      ctgggggac cccaaggca ctggtcgga aatgcttgt 
      gtggtcaat gggagtgtt tatgggccg ggatcggca 
      atggattcc tccactttt tgcgaatat tgctactgt 
      ctgagagga aaacagatc tgtgttaag cccaagtgt 
      ttgcttccg gttgatggg tgtgtgccc atgtatgag 
      gagaccaac tgttgtcca gtgcactat aattgcacg 
      tatgacaca cctagcact agcaccacg accgtgcaa 
      accactcaa ctggagctc aatcaaggt ggttgcatg 
      gtggatggg gtttattac aaagaaggg ggcaaagtt 
      ctcggaatc ggttattcc gtctgtgat aattgttac 
      tgtttacgt ggaatttta cggtgtgag cccttatca 
      tgtgcccct ccacttttt gggtgcact ccggtgata 
      aggcctgga gaatgttgt gccgctagt tacaattgc 
      agtggtacc attgaaatt caaccggaa cccaactac 
      ggccattat cccatcatc agtaaagac tacgcaaaa 
      ttccggaag gaagtccat gacaagaac agcgtgaca 
      gtaccaacc atcaagaac caacgaact catggctcg 
      actcgacat ttcacaggt ccagctttc agctctagt 
      accgtaaaa ccgttcttt tacaactcg attacgaca 
      caagggtcg aaaattttg ccgaaagtt gatcaaaat 
      tcgctcgat gatcgatta aatcgtaga gtggaaaca 
      attgaaacg actgaaatg acttcaact gttgataca 
      acaaccatg actgaagtg ttcagtact gaaacgacc 
      gaaacgact gaaagtgaa tctacgact gaatttacg 
      acaactgag tcgtttcca atcacttta aaaactgtg 
      cttaattcg accgattgt acgaatttg aacgaaaaa 
      agtgatagt tttgaactg gttgagact acgacgctt 
      ccaacgact acaagtgtt gatttggaa aaacaggaa 
      actgtaatt ttcaccacc aaaattcat acacaaaat 
      acgactccg gccctagat cttgctctc atttacaac 
      attacgaaa agtaaagat ccggattat gagtatgac 
      tacagcgag ccctcgcta cccccatct ctaccaaat 
      ttaagaatc atccccttc gtcgcagcg gacgccctc 
      gacttggaa aacgaaaaa cacaacgta atccacccc 
      caagagcgt gtcaccgat atctcacac acttacaac 
      ctgttctca ccccctgtg gaaacagag ggcggattc 
      atccccaaa caacccccg attttggac aatttgtat 
      gacgaaatt gtcacaaca acaacgaca atttcgcca 
      caattgcgt gaaattact tgcattctg gacgaacaa 
      gaaatcaat catggagag tcaatagtt tccgaaaca 
      acatgcaac acttgtggc tgtttttac ggcaacatt 
      gtgtgccag aaaaccatc tgtcctata ccgaaaatc 
      gactgccag aagttttta ttacaagat ccgactacg 
      tgttgccca ctttttgca tgtgacaat gagctcccg 
      actgtggtc ctggaccgc atggactcg ccggaaacg 
      gtgactccg gacccgttc cgggacgtg atccgaacc 
      gagcccgcc cccaacctc caatcgctg attgtggac 
      gttttaaag cgcaaaaca accaccgaa aaaccgttc 
      agtttggac aaagtcttg caattgttg ttctcaacc 
      gaggaagaa gttacaaca acgacgagt acaacaaca 
      acttccaca acaacacag cgactttcg acgaccgaa 
      ttcgactca aatacggtc gactccagc acttcaaac 
      gtcggaatt ttgaaactg gcgggctgt aatatttac 
      ggaaggatg tatcgggtc ggaaggata atctccgaa 
      ttgtcgaat ccttgcttg gagtgcagg tgcactgag 
      accggggtg caatgtcgg cctttgaaa tgctag
    mRNA sequence
    • >TC011603 RA
      ttcggcgga gctccgcca acatgcctc gcgagtgga 
      gtggccttc ctgtggccg tgttgtgtg cctttggag 
      tcatttttg ctgatgcag cctgctggt tcaactcgg 
      aactccacg acgaaggcg tcgaggtgt ccaccaccg 
      agccttgcc tcaactgca cctgcagcc ggggcacgc 
      ttttgtgct acttgcgcg tctgccccc agctgccca 
      acccgccgc ccgccggct gcatcctcc tccatcgct 
      accacacct gctgccctg aactcatct gcacagaca 
      gttttgaag gcagcaacg gcttggagg cgcgctccg 
      aaccgaacg aggaattcg acctggggg accccaagg 
      cactggtcg gaaatgctt gtgtggtca atgggagtg 
      tttatgggc cgggatcgg caatggatt cctccactt 
      tttgcgaat attgctact gtctgagag gaaaacaga 
      tctgtgtta agcccaagt gtttgcttc cggttgatg 
      ggtgtgtgc ccatgtatg aggagacca actgttgtc 
      cagtgcact ataattgca cgtatgaca cacctagca 
      ctagcacca cgaccgtgc aaaccactc aactggagc 
      tcaatcaag gtggttgca tggtggatg gggtttatt 
      acaaagaag ggggcaaag ttctcggaa tcggttatt 
      ccgtctgtg ataattgtt actgtttac gtggaattt 
      tacggtgtg agcccttat catgtgccc ctccacttt 
      ttgggtgca ctccggtga taaggcctg gagaatgtt 
      gtgccgcta gttacaatt gcagtggta ccattgaaa 
      ttcaaccgg aacccaact acggccatt atcccatca 
      tcagtaaag actacgcaa aattccgga aggaagtcc 
      atgacaaga acagcgtga cagtaccaa ccatcaaga 
      accaacgaa ctcatggct cgactcgac atttcacag 
      gtccagctt tcagctcta gtaccgtaa aaccgttct 
      tttacaact cgattacga cacaagggt cgaaaattt 
      tgccgaaag ttgatcaaa attcgctcg atgatcgat 
      taaatcgta gagtggaaa caattgaaa cgactgaaa 
      tgacttcaa ctgttgata caacaacca tgactgaag 
      tgttcagta ctgaaacga ccgaaacga ctgaaagtg 
      aatctacga ctgaattta cgacaactg agtcgtttc 
      caatcactt taaaaactg tgcttaatt cgaccgatt 
      gtacgaatt tgaacgaaa aaagtgata gttttgaac 
      tggttgaga ctacgacgc ttccaacga ctacaagtg 
      ttgatttgg aaaaacagg aaactgtaa ttttcacca 
      ccaaaattc atacacaaa atacgactc cggccctag 
      atcttgctc tcatttaca acattacga aaagtaaag 
      atccggatt atgagtatg actacagcg agccctcgc 
      tacccccat ctctaccaa atttaagaa tcatcccct 
      tcgtcgcag cggacgccc tcgacttgg aaaacgaaa 
      aacacaacg taatccacc cccaagagc gtgtcaccg 
      atatctcac acacttaca acctgttct caccccctg 
      tggaaacag agggcggat tcatcccca aacaacccc 
      cgattttgg acaatttgt atgacgaaa ttgtcacaa 
      caacaacga caatttcgc cacaattgc gtgaaatta 
      cttgcattc tggacgaac aagaaatca atcatggag 
      agtcaatag tttccgaaa caacatgca acacttgtg 
      gctgttttt acggcaaca ttgtgtgcc agaaaacca 
      tctgtccta taccgaaaa tcgactgcc agaagtttt 
      tattacaag atccgacta cgtgttgcc cactttttg 
      catgtgaca atgagctcc cgactgtgg tcctggacc 
      gcatggact cgccggaaa cggtgactc cggacccgt 
      tccgggacg tgatccgaa ccgagcccg cccccaacc 
      tccaatcgc tgattgtgg acgttttaa agcgcaaaa 
      caaccaccg aaaaaccgt tcagtttgg acaaagtct 
      tgcaattgt tgttctcaa ccgaggaag aagttacaa 
      caacgacga gtacaacaa caacttcca caacaacac 
      agcgacttt cgacgaccg aattcgact caaatacgg 
      tcgactcca gcacttcaa acgtcggaa ttttgaaac 
      tggcgggct gtaatattt acggaagga tgtatcggg 
      tcggaagga taatctccg aattgtcga atccttgct 
      tggagtgca ggtgcactg agaccgggg tgcaatgtc 
      ggcctttga aatgctaga tctcactca ctttatacc 
      taagaaagt ccagcagaa agggttcaa atcaaaata 
      atagttttt gaaaaaaat tgcgaaaac gtggagtca 
      acgaatatt tttcaacct cgagaatta gttttacag 
      acttttatt tacataatt ttactaaaa atgttcttt 
      tgtttgtta tttttttta ttgaaatta acagagata 
      agctgagtg gaagtagct aaagtattt tgatttaaa 
      ggcttagtg actggacta aaatacgta tttgttcac 
      gtattgtag tattaattt aattcaatt taatttaac 
      tctcacaac cgcaatcac cttgacagg tgccaagta 
      caaatttct atttgccat ggaacacgt tcagatatt 
      ctgccgata tttcattac atctaattt agtttttaa 
      ttattttgg ttaattatt acgtgtgtg atattggtc 
      ttgtaggat ttttttacc aaaaattta aacgaattt 
      tttaattgt tttgagcgg ataataagg tgaaaaaat 
      gtaggtaaa ctgtaggaa atatcagac aatttttat 
      acactgcaa tttgaggct attcaacca acttacgtt 
      ttatgtaat tattaaaaa taaaaatca aagaaatgg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY