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Gene information
General information
TC011603 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15226203..15235715
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC011603
    iBeetle screen iB_05059

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011603 RA
      MPREWSGLP VAVLCAFGV IFADAACWF NSELHDEGV 
EVSTTEPCL NCTCSRGTL LCYLRVCPQ LPNPPPAGC 
ILLHRYHTC CPELICTDS FEGSNGLEA RSEPNEEFD 
LGDPKALVG NACVVNGSV YGPGSAMDS STFCEYCYC 
LRGKQICVK PKCLLPVDG CVPMYEETN CCPVHYNCT 
YDTPSTSTT TVQTTQLEL NQGGCMVDG VYYKEGGKV 
LGIGYSVCD NCYCLRGIL RCEPLSCAP PLFGCTPVI 
RPGECCAAS YNCSGTIEI QPEPNYGHY PIISKDYAK 
FRKEVHDKN SVTVPTIKN QRTHGSTRH FTGPAFSSS 
TVKPFFYNS ITTQGSKIL PKVDQNSLD DRLNRRVET 
IETTEMTST VDTTTMTEV FSTETTETT ESESTTEFT 
TTESFPITL KTVLNSTDC TNLNEKSDS FELVETTTL 
PTTTSVDLE KQETVIFTT KIHTQNTTP ALDLALIYN 
ITKSKDPDY EYDYSEPSL PPSLPNLRI IPFVAADAL 
DLENEKHNV IHPQERVTD ISHTYNLFS PPVETEGGF 
IPKQPPILD NLYDEIVTT TTTISPQLR EITCILDEQ 
EINHGESIV SETTCNTCG CFYGNIVCQ KTICPIPKI 
DCQKFLLQD PTTCCPLFA CDNELPTVV LDRMDSPET 
VTPDPFRDV IRTEPAPNL QSLIVDVLK RKTTTEKPF 
SLDKVLQLL FSTEEEVTT TTSTTTTST TTQRLSTTE 
FDSNTVDSS TSNVGILKL AGCNIYGRM YRVGRIISE 
LSNPCLECR CTETGVQCR PLKC
    CDS sequence
    • >TC011603 RA
      atgcctcgc gagtggagt ggccttcct gtggccgtg 
ttgtgtgcc tttggagtc atttttgct gatgcagcc 
tgctggttc aactcggaa ctccacgac gaaggcgtc 
gaggtgtcc accaccgag ccttgcctc aactgcacc 
tgcagccgg ggcacgctt ttgtgctac ttgcgcgtc 
tgcccccag ctgcccaac ccgccgccc gccggctgc 
atcctcctc catcgctac cacacctgc tgccctgaa 
ctcatctgc acagacagt tttgaaggc agcaacggc 
ttggaggcg cgctccgaa ccgaacgag gaattcgac 
ctgggggac cccaaggca ctggtcgga aatgcttgt 
gtggtcaat gggagtgtt tatgggccg ggatcggca 
atggattcc tccactttt tgcgaatat tgctactgt 
ctgagagga aaacagatc tgtgttaag cccaagtgt 
ttgcttccg gttgatggg tgtgtgccc atgtatgag 
gagaccaac tgttgtcca gtgcactat aattgcacg 
tatgacaca cctagcact agcaccacg accgtgcaa 
accactcaa ctggagctc aatcaaggt ggttgcatg 
gtggatggg gtttattac aaagaaggg ggcaaagtt 
ctcggaatc ggttattcc gtctgtgat aattgttac 
tgtttacgt ggaatttta cggtgtgag cccttatca 
tgtgcccct ccacttttt gggtgcact ccggtgata 
aggcctgga gaatgttgt gccgctagt tacaattgc 
agtggtacc attgaaatt caaccggaa cccaactac 
ggccattat cccatcatc agtaaagac tacgcaaaa 
ttccggaag gaagtccat gacaagaac agcgtgaca 
gtaccaacc atcaagaac caacgaact catggctcg 
actcgacat ttcacaggt ccagctttc agctctagt 
accgtaaaa ccgttcttt tacaactcg attacgaca 
caagggtcg aaaattttg ccgaaagtt gatcaaaat 
tcgctcgat gatcgatta aatcgtaga gtggaaaca 
attgaaacg actgaaatg acttcaact gttgataca 
acaaccatg actgaagtg ttcagtact gaaacgacc 
gaaacgact gaaagtgaa tctacgact gaatttacg 
acaactgag tcgtttcca atcacttta aaaactgtg 
cttaattcg accgattgt acgaatttg aacgaaaaa 
agtgatagt tttgaactg gttgagact acgacgctt 
ccaacgact acaagtgtt gatttggaa aaacaggaa 
actgtaatt ttcaccacc aaaattcat acacaaaat 
acgactccg gccctagat cttgctctc atttacaac 
attacgaaa agtaaagat ccggattat gagtatgac 
tacagcgag ccctcgcta cccccatct ctaccaaat 
ttaagaatc atccccttc gtcgcagcg gacgccctc 
gacttggaa aacgaaaaa cacaacgta atccacccc 
caagagcgt gtcaccgat atctcacac acttacaac 
ctgttctca ccccctgtg gaaacagag ggcggattc 
atccccaaa caacccccg attttggac aatttgtat 
gacgaaatt gtcacaaca acaacgaca atttcgcca 
caattgcgt gaaattact tgcattctg gacgaacaa 
gaaatcaat catggagag tcaatagtt tccgaaaca 
acatgcaac acttgtggc tgtttttac ggcaacatt 
gtgtgccag aaaaccatc tgtcctata ccgaaaatc 
gactgccag aagttttta ttacaagat ccgactacg 
tgttgccca ctttttgca tgtgacaat gagctcccg 
actgtggtc ctggaccgc atggactcg ccggaaacg 
gtgactccg gacccgttc cgggacgtg atccgaacc 
gagcccgcc cccaacctc caatcgctg attgtggac 
gttttaaag cgcaaaaca accaccgaa aaaccgttc 
agtttggac aaagtcttg caattgttg ttctcaacc 
gaggaagaa gttacaaca acgacgagt acaacaaca 
acttccaca acaacacag cgactttcg acgaccgaa 
ttcgactca aatacggtc gactccagc acttcaaac 
gtcggaatt ttgaaactg gcgggctgt aatatttac 
ggaaggatg tatcgggtc ggaaggata atctccgaa 
ttgtcgaat ccttgcttg gagtgcagg tgcactgag 
accggggtg caatgtcgg cctttgaaa tgctag
    mRNA sequence
    • >TC011603 RA
      ttcggcgga gctccgcca acatgcctc gcgagtgga 
gtggccttc ctgtggccg tgttgtgtg cctttggag 
tcatttttg ctgatgcag cctgctggt tcaactcgg 
aactccacg acgaaggcg tcgaggtgt ccaccaccg 
agccttgcc tcaactgca cctgcagcc ggggcacgc 
ttttgtgct acttgcgcg tctgccccc agctgccca 
acccgccgc ccgccggct gcatcctcc tccatcgct 
accacacct gctgccctg aactcatct gcacagaca 
gttttgaag gcagcaacg gcttggagg cgcgctccg 
aaccgaacg aggaattcg acctggggg accccaagg 
cactggtcg gaaatgctt gtgtggtca atgggagtg 
tttatgggc cgggatcgg caatggatt cctccactt 
tttgcgaat attgctact gtctgagag gaaaacaga 
tctgtgtta agcccaagt gtttgcttc cggttgatg 
ggtgtgtgc ccatgtatg aggagacca actgttgtc 
cagtgcact ataattgca cgtatgaca cacctagca 
ctagcacca cgaccgtgc aaaccactc aactggagc 
tcaatcaag gtggttgca tggtggatg gggtttatt 
acaaagaag ggggcaaag ttctcggaa tcggttatt 
ccgtctgtg ataattgtt actgtttac gtggaattt 
tacggtgtg agcccttat catgtgccc ctccacttt 
ttgggtgca ctccggtga taaggcctg gagaatgtt 
gtgccgcta gttacaatt gcagtggta ccattgaaa 
ttcaaccgg aacccaact acggccatt atcccatca 
tcagtaaag actacgcaa aattccgga aggaagtcc 
atgacaaga acagcgtga cagtaccaa ccatcaaga 
accaacgaa ctcatggct cgactcgac atttcacag 
gtccagctt tcagctcta gtaccgtaa aaccgttct 
tttacaact cgattacga cacaagggt cgaaaattt 
tgccgaaag ttgatcaaa attcgctcg atgatcgat 
taaatcgta gagtggaaa caattgaaa cgactgaaa 
tgacttcaa ctgttgata caacaacca tgactgaag 
tgttcagta ctgaaacga ccgaaacga ctgaaagtg 
aatctacga ctgaattta cgacaactg agtcgtttc 
caatcactt taaaaactg tgcttaatt cgaccgatt 
gtacgaatt tgaacgaaa aaagtgata gttttgaac 
tggttgaga ctacgacgc ttccaacga ctacaagtg 
ttgatttgg aaaaacagg aaactgtaa ttttcacca 
ccaaaattc atacacaaa atacgactc cggccctag 
atcttgctc tcatttaca acattacga aaagtaaag 
atccggatt atgagtatg actacagcg agccctcgc 
tacccccat ctctaccaa atttaagaa tcatcccct 
tcgtcgcag cggacgccc tcgacttgg aaaacgaaa 
aacacaacg taatccacc cccaagagc gtgtcaccg 
atatctcac acacttaca acctgttct caccccctg 
tggaaacag agggcggat tcatcccca aacaacccc 
cgattttgg acaatttgt atgacgaaa ttgtcacaa 
caacaacga caatttcgc cacaattgc gtgaaatta 
cttgcattc tggacgaac aagaaatca atcatggag 
agtcaatag tttccgaaa caacatgca acacttgtg 
gctgttttt acggcaaca ttgtgtgcc agaaaacca 
tctgtccta taccgaaaa tcgactgcc agaagtttt 
tattacaag atccgacta cgtgttgcc cactttttg 
catgtgaca atgagctcc cgactgtgg tcctggacc 
gcatggact cgccggaaa cggtgactc cggacccgt 
tccgggacg tgatccgaa ccgagcccg cccccaacc 
tccaatcgc tgattgtgg acgttttaa agcgcaaaa 
caaccaccg aaaaaccgt tcagtttgg acaaagtct 
tgcaattgt tgttctcaa ccgaggaag aagttacaa 
caacgacga gtacaacaa caacttcca caacaacac 
agcgacttt cgacgaccg aattcgact caaatacgg 
tcgactcca gcacttcaa acgtcggaa ttttgaaac 
tggcgggct gtaatattt acggaagga tgtatcggg 
tcggaagga taatctccg aattgtcga atccttgct 
tggagtgca ggtgcactg agaccgggg tgcaatgtc 
ggcctttga aatgctaga tctcactca ctttatacc 
taagaaagt ccagcagaa agggttcaa atcaaaata 
atagttttt gaaaaaaat tgcgaaaac gtggagtca 
acgaatatt tttcaacct cgagaatta gttttacag 
acttttatt tacataatt ttactaaaa atgttcttt 
tgtttgtta tttttttta ttgaaatta acagagata 
agctgagtg gaagtagct aaagtattt tgatttaaa 
ggcttagtg actggacta aaatacgta tttgttcac 
gtattgtag tattaattt aattcaatt taatttaac 
tctcacaac cgcaatcac cttgacagg tgccaagta 
caaatttct atttgccat ggaacacgt tcagatatt 
ctgccgata tttcattac atctaattt agtttttaa 
ttattttgg ttaattatt acgtgtgtg atattggtc 
ttgtaggat ttttttacc aaaaattta aacgaattt 
tttaattgt tttgagcgg ataataagg tgaaaaaat 
gtaggtaaa ctgtaggaa atatcagac aatttttat 
acactgcaa tttgaggct attcaacca acttacgtt 
ttatgtaat tattaaaaa taaaaatca aagaaatgg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY