| General information | |||
| TC011603 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 15226203..15235715 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC011603 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05059 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (19 dpi)
- >TC011603 RA
MPREWSGLP VAVLCAFGV IFADAACWF NSELHDEGV EVSTTEPCL NCTCSRGTL LCYLRVCPQ LPNPPPAGC ILLHRYHTC CPELICTDS FEGSNGLEA RSEPNEEFD LGDPKALVG NACVVNGSV YGPGSAMDS STFCEYCYC LRGKQICVK PKCLLPVDG CVPMYEETN CCPVHYNCT YDTPSTSTT TVQTTQLEL NQGGCMVDG VYYKEGGKV LGIGYSVCD NCYCLRGIL RCEPLSCAP PLFGCTPVI RPGECCAAS YNCSGTIEI QPEPNYGHY PIISKDYAK FRKEVHDKN SVTVPTIKN QRTHGSTRH FTGPAFSSS TVKPFFYNS ITTQGSKIL PKVDQNSLD DRLNRRVET IETTEMTST VDTTTMTEV FSTETTETT ESESTTEFT TTESFPITL KTVLNSTDC TNLNEKSDS FELVETTTL PTTTSVDLE KQETVIFTT KIHTQNTTP ALDLALIYN ITKSKDPDY EYDYSEPSL PPSLPNLRI IPFVAADAL DLENEKHNV IHPQERVTD ISHTYNLFS PPVETEGGF IPKQPPILD NLYDEIVTT TTTISPQLR EITCILDEQ EINHGESIV SETTCNTCG CFYGNIVCQ KTICPIPKI DCQKFLLQD PTTCCPLFA CDNELPTVV LDRMDSPET VTPDPFRDV IRTEPAPNL QSLIVDVLK RKTTTEKPF SLDKVLQLL FSTEEEVTT TTSTTTTST TTQRLSTTE FDSNTVDSS TSNVGILKL AGCNIYGRM YRVGRIISE LSNPCLECR CTETGVQCR PLKC
- >TC011603 RA
atgcctcgc gagtggagt ggccttcct gtggccgtg ttgtgtgcc tttggagtc atttttgct gatgcagcc tgctggttc aactcggaa ctccacgac gaaggcgtc gaggtgtcc accaccgag ccttgcctc aactgcacc tgcagccgg ggcacgctt ttgtgctac ttgcgcgtc tgcccccag ctgcccaac ccgccgccc gccggctgc atcctcctc catcgctac cacacctgc tgccctgaa ctcatctgc acagacagt tttgaaggc agcaacggc ttggaggcg cgctccgaa ccgaacgag gaattcgac ctgggggac cccaaggca ctggtcgga aatgcttgt gtggtcaat gggagtgtt tatgggccg ggatcggca atggattcc tccactttt tgcgaatat tgctactgt ctgagagga aaacagatc tgtgttaag cccaagtgt ttgcttccg gttgatggg tgtgtgccc atgtatgag gagaccaac tgttgtcca gtgcactat aattgcacg tatgacaca cctagcact agcaccacg accgtgcaa accactcaa ctggagctc aatcaaggt ggttgcatg gtggatggg gtttattac aaagaaggg ggcaaagtt ctcggaatc ggttattcc gtctgtgat aattgttac tgtttacgt ggaatttta cggtgtgag cccttatca tgtgcccct ccacttttt gggtgcact ccggtgata aggcctgga gaatgttgt gccgctagt tacaattgc agtggtacc attgaaatt caaccggaa cccaactac ggccattat cccatcatc agtaaagac tacgcaaaa ttccggaag gaagtccat gacaagaac agcgtgaca gtaccaacc atcaagaac caacgaact catggctcg actcgacat ttcacaggt ccagctttc agctctagt accgtaaaa ccgttcttt tacaactcg attacgaca caagggtcg aaaattttg ccgaaagtt gatcaaaat tcgctcgat gatcgatta aatcgtaga gtggaaaca attgaaacg actgaaatg acttcaact gttgataca acaaccatg actgaagtg ttcagtact gaaacgacc gaaacgact gaaagtgaa tctacgact gaatttacg acaactgag tcgtttcca atcacttta aaaactgtg cttaattcg accgattgt acgaatttg aacgaaaaa agtgatagt tttgaactg gttgagact acgacgctt ccaacgact acaagtgtt gatttggaa aaacaggaa actgtaatt ttcaccacc aaaattcat acacaaaat acgactccg gccctagat cttgctctc atttacaac attacgaaa agtaaagat ccggattat gagtatgac tacagcgag ccctcgcta cccccatct ctaccaaat ttaagaatc atccccttc gtcgcagcg gacgccctc gacttggaa aacgaaaaa cacaacgta atccacccc caagagcgt gtcaccgat atctcacac acttacaac ctgttctca ccccctgtg gaaacagag ggcggattc atccccaaa caacccccg attttggac aatttgtat gacgaaatt gtcacaaca acaacgaca atttcgcca caattgcgt gaaattact tgcattctg gacgaacaa gaaatcaat catggagag tcaatagtt tccgaaaca acatgcaac acttgtggc tgtttttac ggcaacatt gtgtgccag aaaaccatc tgtcctata ccgaaaatc gactgccag aagttttta ttacaagat ccgactacg tgttgccca ctttttgca tgtgacaat gagctcccg actgtggtc ctggaccgc atggactcg ccggaaacg gtgactccg gacccgttc cgggacgtg atccgaacc gagcccgcc cccaacctc caatcgctg attgtggac gttttaaag cgcaaaaca accaccgaa aaaccgttc agtttggac aaagtcttg caattgttg ttctcaacc gaggaagaa gttacaaca acgacgagt acaacaaca acttccaca acaacacag cgactttcg acgaccgaa ttcgactca aatacggtc gactccagc acttcaaac gtcggaatt ttgaaactg gcgggctgt aatatttac ggaaggatg tatcgggtc ggaaggata atctccgaa ttgtcgaat ccttgcttg gagtgcagg tgcactgag accggggtg caatgtcgg cctttgaaa tgctag
- >TC011603 RA
ttcggcgga gctccgcca acatgcctc gcgagtgga gtggccttc ctgtggccg tgttgtgtg cctttggag tcatttttg ctgatgcag cctgctggt tcaactcgg aactccacg acgaaggcg tcgaggtgt ccaccaccg agccttgcc tcaactgca cctgcagcc ggggcacgc ttttgtgct acttgcgcg tctgccccc agctgccca acccgccgc ccgccggct gcatcctcc tccatcgct accacacct gctgccctg aactcatct gcacagaca gttttgaag gcagcaacg gcttggagg cgcgctccg aaccgaacg aggaattcg acctggggg accccaagg cactggtcg gaaatgctt gtgtggtca atgggagtg tttatgggc cgggatcgg caatggatt cctccactt tttgcgaat attgctact gtctgagag gaaaacaga tctgtgtta agcccaagt gtttgcttc cggttgatg ggtgtgtgc ccatgtatg aggagacca actgttgtc cagtgcact ataattgca cgtatgaca cacctagca ctagcacca cgaccgtgc aaaccactc aactggagc tcaatcaag gtggttgca tggtggatg gggtttatt acaaagaag ggggcaaag ttctcggaa tcggttatt ccgtctgtg ataattgtt actgtttac gtggaattt tacggtgtg agcccttat catgtgccc ctccacttt ttgggtgca ctccggtga taaggcctg gagaatgtt gtgccgcta gttacaatt gcagtggta ccattgaaa ttcaaccgg aacccaact acggccatt atcccatca tcagtaaag actacgcaa aattccgga aggaagtcc atgacaaga acagcgtga cagtaccaa ccatcaaga accaacgaa ctcatggct cgactcgac atttcacag gtccagctt tcagctcta gtaccgtaa aaccgttct tttacaact cgattacga cacaagggt cgaaaattt tgccgaaag ttgatcaaa attcgctcg atgatcgat taaatcgta gagtggaaa caattgaaa cgactgaaa tgacttcaa ctgttgata caacaacca tgactgaag tgttcagta ctgaaacga ccgaaacga ctgaaagtg aatctacga ctgaattta cgacaactg agtcgtttc caatcactt taaaaactg tgcttaatt cgaccgatt gtacgaatt tgaacgaaa aaagtgata gttttgaac tggttgaga ctacgacgc ttccaacga ctacaagtg ttgatttgg aaaaacagg aaactgtaa ttttcacca ccaaaattc atacacaaa atacgactc cggccctag atcttgctc tcatttaca acattacga aaagtaaag atccggatt atgagtatg actacagcg agccctcgc tacccccat ctctaccaa atttaagaa tcatcccct tcgtcgcag cggacgccc tcgacttgg aaaacgaaa aacacaacg taatccacc cccaagagc gtgtcaccg atatctcac acacttaca acctgttct caccccctg tggaaacag agggcggat tcatcccca aacaacccc cgattttgg acaatttgt atgacgaaa ttgtcacaa caacaacga caatttcgc cacaattgc gtgaaatta cttgcattc tggacgaac aagaaatca atcatggag agtcaatag tttccgaaa caacatgca acacttgtg gctgttttt acggcaaca ttgtgtgcc agaaaacca tctgtccta taccgaaaa tcgactgcc agaagtttt tattacaag atccgacta cgtgttgcc cactttttg catgtgaca atgagctcc cgactgtgg tcctggacc gcatggact cgccggaaa cggtgactc cggacccgt tccgggacg tgatccgaa ccgagcccg cccccaacc tccaatcgc tgattgtgg acgttttaa agcgcaaaa caaccaccg aaaaaccgt tcagtttgg acaaagtct tgcaattgt tgttctcaa ccgaggaag aagttacaa caacgacga gtacaacaa caacttcca caacaacac agcgacttt cgacgaccg aattcgact caaatacgg tcgactcca gcacttcaa acgtcggaa ttttgaaac tggcgggct gtaatattt acggaagga tgtatcggg tcggaagga taatctccg aattgtcga atccttgct tggagtgca ggtgcactg agaccgggg tgcaatgtc ggcctttga aatgctaga tctcactca ctttatacc taagaaagt ccagcagaa agggttcaa atcaaaata atagttttt gaaaaaaat tgcgaaaac gtggagtca acgaatatt tttcaacct cgagaatta gttttacag acttttatt tacataatt ttactaaaa atgttcttt tgtttgtta tttttttta ttgaaatta acagagata agctgagtg gaagtagct aaagtattt tgatttaaa ggcttagtg actggacta aaatacgta tttgttcac gtattgtag tattaattt aattcaatt taatttaac tctcacaac cgcaatcac cttgacagg tgccaagta caaatttct atttgccat ggaacacgt tcagatatt ctgccgata tttcattac atctaattt agtttttaa ttattttgg ttaattatt acgtgtgtg atattggtc ttgtaggat ttttttacc aaaaattta aacgaattt tttaattgt tttgagcgg ataataagg tgaaaaaat gtaggtaaa ctgtaggaa atatcagac aatttttat acactgcaa tttgaggct attcaacca acttacgtt ttatgtaat tattaaaaa taaaaatca aagaaatgg
