Status
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Gene information
General information
TC011565 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450689.1: 70790..71960
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011565
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05057

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 8 (19 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    Adult morphology
    elytra not closed - Number of animals showing the phenotype: 5


    elytra surface irregular - Number of animals showing the phenotype: 7


    hindwing blistered - Number of animals showing the phenotype: 5


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011565 RA
      MSSIERIKQ NADLAKQTI QELKNELNF LNKEYNNIL 
      ARELREENA RLLAAVEEA KAHLVKLEV KNGIPQIPV 
      PNQTSRPVT TTAVGSSEP PKEEPPKAQ KEPKPKKDK 
      KPKEASLEP PVDIGRLDL RIAKVEDVQ KHPDADSLY 
      VLKINCGED KPRTVCSGL VKHVPIEEL RDRTVMLLC 
      NLKPVKMRG ITSEAMVMC ASSDQGVEV LTPPSGCTP 
      GEPVHCEGY TRQPDPVMN PKKKIFETV APDLLTDAT 
      LRACYKGVP LTVPHKGAI VAKSLANVP VK
    • >TC011565 RB
      MLLNIFRIN VRNVLKMSS IERIKQNAD LAKQTIQEL 
      KNELNFLNK EYNNILARE LREENARLL AAVEEAKAH 
      LVKLEVKNG IPQIPVPNQ TSRPVTTTA VGSSEPPKE 
      EPPKAQKEP KPKKDKKPK EASLEPPVD IGRLDLRIA 
      KVEDVQKHP DADSLYVLK INCGEDKPR TVCSGLVKH 
      VPIEELRDR TVMLLCNLK PVKMRGITS EAMVMCASS 
      DQGVEVLTP PSGCTPGEP VHCEGYTRQ PDPVMNPKK 
      KIFETVAPD LLTDATLRA CYKGVPLTV PHKGAIVAK 
      SLANVPVK
    CDS sequence
    • >TC011565 RA
      atgtcgtca atcgaacgc attaaacaa aacgcagac 
      ctggcaaag caaacaatc caggaactt aaaaatgag 
      ttaaatttt ctcaacaag gaatacaac aatattctg 
      gcccgcgaa ctgcgtgag gaaaacgca cgtctgctt 
      gccgcagtg gaggaggca aaagcacac ctcgtgaaa 
      ctagaagtg aaaaatggg attccccaa atcccggtc 
      cctaaccaa acctcacgt cctgtcacc accactgcg 
      gtgggatcg tcagagccc cccaaggag gagcctcca 
      aaggcgcaa aaagagccc aaacccaag aaagacaag 
      aagccgaag gaggcctcc cttgagccc ccagtggac 
      atcgggcgc ctcgacctc cgcattgcg aaagtcgag 
      gacgtgcaa aagcacccc gatgccgac tctctctac 
      gttttgaaa atcaactgt ggggaagac aagccaagg 
      accgtgtgc tccggtttg gtcaaacac gtccccatt 
      gaggagcta cgtgatcgt actgtgatg cttttgtgt 
      aatctcaaa ccggttaaa atgcgggga atcacctcc 
      gaggccatg gtcatgtgc gctagcagt gaccaaggg 
      gtcgaagtc ctaaccccg ccgtctggg tgcaccccc 
      ggcgagccc gtccattgt gagggctac acccgacaa 
      cccgacccg gtcatgaac cccaaaaag aaaattttc 
      gaaacggtg gcccccgac ttactcacg gacgccacc 
      ttgcgggcg tgctacaag ggggtgccg ctcacggtg 
      ccccacaag ggggccatc gtggccaaa agcctcgct 
      aatgttcca gtgaaataa
    • >TC011565 RB
      atgttgtta aatattttt agaataaac gtgcgaaac 
      gtgcttaaa atgtcgtca atcgaacgc attaaacaa 
      aacgcagac ctggcaaag caaacaatc caggaactt 
      aaaaatgag ttaaatttt ctcaacaag gaatacaac 
      aatattctg gcccgcgaa ctgcgtgag gaaaacgca 
      cgtctgctt gccgcagtg gaggaggca aaagcacac 
      ctcgtgaaa ctagaagtg aaaaatggg attccccaa 
      atcccggtc cctaaccaa acctcacgt cctgtcacc 
      accactgcg gtgggatcg tcagagccc cccaaggag 
      gagcctcca aaggcgcaa aaagagccc aaacccaag 
      aaagacaag aagccgaag gaggcctcc cttgagccc 
      ccagtggac atcgggcgc ctcgacctc cgcattgcg 
      aaagtcgag gacgtgcaa aagcacccc gatgccgac 
      tctctctac gttttgaaa atcaactgt ggggaagac 
      aagccaagg accgtgtgc tccggtttg gtcaaacac 
      gtccccatt gaggagcta cgtgatcgt actgtgatg 
      cttttgtgt aatctcaaa ccggttaaa atgcgggga 
      atcacctcc gaggccatg gtcatgtgc gctagcagt 
      gaccaaggg gtcgaagtc ctaaccccg ccgtctggg 
      tgcaccccc ggcgagccc gtccattgt gagggctac 
      acccgacaa cccgacccg gtcatgaac cccaaaaag 
      aaaattttc gaaacggtg gcccccgac ttactcacg 
      gacgccacc ttgcgggcg tgctacaag ggggtgccg 
      ctcacggtg ccccacaag ggggccatc gtggccaaa 
      agcctcgct aatgttcca gtgaaataa
    mRNA sequence
    • >TC011565 RA
      tgattttag tgttgccgg gttgtttaa acaagtcca 
      tcttttccc aaatttcgc tctgtcgcg aattcgcac 
      tcacacttt attttagaa taaacgtgc gaaacgtgc 
      ttaaaatgt cgtcaatcg aacgcatta aacaaaacg 
      cagacctgg caaagcaaa caatccagg aacttaaaa 
      atgagttaa attttctca acaaggaat acaacaata 
      ttctggccc gcgaactgc gtgaggaaa acgcacgtc 
      tgcttgccg cagtggagg aggcaaaag cacacctcg 
      tgaaactag aagtgaaaa atgggattc cccaaatcc 
      cggtcccta accaaacct cacgtcctg tcaccacca 
      ctgcggtgg gatcgtcag agcccccca aggaggagc 
      ctccaaagg cgcaaaaag agcccaaac ccaagaaag 
      acaagaagc cgaaggagg cctcccttg agcccccag 
      tggacatcg ggcgcctcg acctccgca ttgcgaaag 
      tcgaggacg tgcaaaagc accccgatg ccgactctc 
      tctacgttt tgaaaatca actgtgggg aagacaagc 
      caaggaccg tgtgctccg gtttggtca aacacgtcc 
      ccattgagg agctacgtg atcgtactg tgatgcttt 
      tgtgtaatc tcaaaccgg ttaaaatgc ggggaatca 
      cctccgagg ccatggtca tgtgcgcta gcagtgacc 
      aaggggtcg aagtcctaa ccccgccgt ctgggtgca 
      cccccggcg agcccgtcc attgtgagg gctacaccc 
      gacaacccg acccggtca tgaacccca aaaagaaaa 
      ttttcgaaa cggtggccc ccgacttac tcacggacg 
      ccaccttgc gggcgtgct acaaggggg tgccgctca 
      cggtgcccc acaaggggg ccatcgtgg ccaaaagcc 
      tcgctaatg ttccagtga aataaaaca acaaatgac 
      acctatatg tttatttat gattaggaa taaattaac 
      tcaacaaat
    • >TC011565 RB
      tgattttag tgttgccgg gttgtttaa acaagtcca 
      tcttttccc aaatttcgc tctgtcgcg aattcgcac 
      tcacacttt attttaggt tagtaaccc ccaagtgaa 
      aaataccgt aattcccct aaatgttgt taaatattt 
      ttagaataa acgtgcgaa acgtgctta aaatgtcgt 
      caatcgaac gcattaaac aaaacgcag acctggcaa 
      agcaaacaa tccaggaac ttaaaaatg agttaaatt 
      ttctcaaca aggaataca acaatattc tggcccgcg 
      aactgcgtg aggaaaacg cacgtctgc ttgccgcag 
      tggaggagg caaaagcac acctcgtga aactagaag 
      tgaaaaatg ggattcccc aaatcccgg tccctaacc 
      aaacctcac gtcctgtca ccaccactg cggtgggat 
      cgtcagagc cccccaagg aggagcctc caaaggcgc 
      aaaaagagc ccaaaccca agaaagaca agaagccga 
      aggaggcct cccttgagc ccccagtgg acatcgggc 
      gcctcgacc tccgcattg cgaaagtcg aggacgtgc 
      aaaagcacc ccgatgccg actctctct acgttttga 
      aaatcaact gtggggaag acaagccaa ggaccgtgt 
      gctccggtt tggtcaaac acgtcccca ttgaggagc 
      tacgtgatc gtactgtga tgcttttgt gtaatctca 
      aaccggtta aaatgcggg gaatcacct ccgaggcca 
      tggtcatgt gcgctagca gtgaccaag gggtcgaag 
      tcctaaccc cgccgtctg ggtgcaccc ccggcgagc 
      ccgtccatt gtgagggct acacccgac aacccgacc 
      cggtcatga accccaaaa agaaaattt tcgaaacgg 
      tggcccccg acttactca cggacgcca ccttgcggg 
      cgtgctaca agggggtgc cgctcacgg tgccccaca 
      agggggcca tcgtggcca aaagcctcg ctaatgttc 
      cagtgaaat aaaacaaca aatgacacc tatatgttt 
      atttatgat taggaataa attaactca acaaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY