Status
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Gene information
General information
TC011558 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450689.1: 75982..79405
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011558
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05056

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    previtellogenic egg chamber potentially not present - Number of animals showing the phenotype: 2

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011558 RA
      MSSPRNPKH QQEGVVKSL RDLFGNIFH IDIIRSVAH 
      QCNFDIVRS SDVLIEMSN NIADQPSKK KQPKNSPKK 
      TDPPRVIKS KKSQELERV INNIRQGYK VLVILRGLP 
      GSGKSQLAV GITDVTLGP GNNSPHILS TDDYFIQNG 
      RYVFDPNRL QEAHEWNHR RAFQAMSRG LSPVIIDNT 
      NTQMWEMKP YAMMATDYG YLIEILEPD TWWCFNDKE 
      LARRNSHGV PRNKIKDML DRYDKNITT QKLLCAYSL 
      TYKFQKPPQ FRLIPPIVR QNGYRIQNG VTKSRSTPI 
      FDRQKSIEN GVEAINLMD FNDVSNETE QNQAGTWDL 
      NPVDAILMC TEENQTDVL QPKPITSIE NAWGIDEKA 
      LYSWDIVTP LAEVGTPVD ATIQPQVPK PETRDACTC 
      YQANDWLNV AHKTVAARN RDINVGTPV RVLPPPKKT 
      MLDKSCITD DVMDEEVSE EERIKDLMD LFPKLPKSS 
      LCELFNKCH KNFHWTVDL LLEDSNLEM AETDDVTTP 
      KVKLFKRKT ISGDSLELK KCLEGKFDI NREFYSDHV 
      YKVKQFKFG SLDPPLPQP STSTSDSDS DADLDPKSM 
      IELNLGATF VAQLESLFG GDDGAFPQG YQPVVQMPL 
      NLARQIYSF YIESVCQQM DAQTHILDR LVKEDEELA 
      RKLQKQEES RLVPQREPN FTEIMDEQV ALSLHQRAV 
      DEWKNMTAD NLAAKLTKQ KLFAAFPNI DQNALLEIW 
      HAYDYDYNK TVEDLIANV GGSLDDQIK EPPLSATTL 
      QEMKQAEEN FDDFEQNEE QKPATYYRE EANKHLKRR 
      VELYERAKL YFQKGMFEV ATFYSELAK KETKLYDKA 
      NHVAAAAFA DEHSKRLQK FDTLDLHYF YVKEAIPNL 
      DIFIDRNIN LLKGGAKNG IDLFVITGR GKNSEGGRC 
      KIKPAVITR LKKRNIGFV QMNPGLLKI RVMRNSLMT 
      SDLP
    CDS sequence
    • >TC011558 RA
      atgtcatcc cccagaaac cccaaacac cagcaagaa 
      ggcgtcgtg aaaagcctc cgcgatctt tttggtaac 
      attttccac atcgatatc atccgttca gtcgcacac 
      caatgcaat ttcgacatc gtaagatcc tccgacgtc 
      ctcatcgaa atgtccaac aacattgcc gaccaaccc 
      tcgaaaaaa aaacaaccg aaaaactcc ccgaaaaaa 
      accgacccg ccccgagtg atcaaatca aaaaaatcg 
      caagaattg gaacgtgtg attaacaat atcagacaa 
      ggctacaag gttttggtg attttgagg ggtttaccg 
      gggagtggc aagtcccaa ctagccgtt ggtatcact 
      gacgtcact ctgggcccg gggaataac agcccacat 
      atcctaagc actgacgat tattttatc caaaacggg 
      agatacgtt tttgaccca aacaggctc caggaagcc 
      cacgagtgg aaccatcgc agggccttc caggccatg 
      agtcggggg ctaagcccc gttataatc gacaacacc 
      aacacacaa atgtgggaa atgaagccc tacgccatg 
      atggccact gattacggc tacttgatt gaaattctc 
      gaacctgac acgtggtgg tgcttcaac gacaaggaa 
      ctagcgcgg agaaacagt cacggggtt ccccgaaac 
      aaaatcaag gacatgttg gaccgatac gacaaaaac 
      ataaccacg caaaaatta ctatgcgcc tatagtctc 
      acttacaag ttccaaaaa ccgccccaa ttccgccta 
      atcccccca attgtacga caaaatggc taccgcatt 
      caaaatggc gtcacgaaa tcgcgaagc acgccgatc 
      ttcgatcgg caaaagtcg atcgaaaac ggcgtcgag 
      gcgatcaat ttgatggat tttaacgac gtttcgaac 
      gaaactgag cagaatcag gccggcacg tgggacctc 
      aaccccgtc gacgccatc ttgatgtgc accgaagag 
      aaccaaacc gacgtctta caaccaaaa cccatcact 
      agcattgaa aacgcctgg ggtattgac gaaaaagcg 
      ctctattcc tgggacatt gtcactcca ctcgccgaa 
      gttggcacc cctgtcgac gccacaatt caaccccaa 
      gtcccaaaa cccgaaaca cgtgacgct tgcacgtgt 
      taccaggcc aacgattgg ctgaacgtt gctcacaaa 
      acagtggcg gcgcgtaac cgcgacatt aatgttggc 
      acacctgtg cgggtttta ccgccaccc aaaaaaaca 
      atgcttgat aagagctgc atcacggac gacgtgatg 
      gacgaggag gtgagcgaa gaggagcga attaaagac 
      ttgatggat ttgttccca aaactaccg aaatcgtcg 
      ctttgtgaa ctgtttaat aaatgtcac aagaacttt 
      cactggact gttgatctt ctcctcgaa gacagtaac 
      cttgaaatg gccgaaacc gatgacgtc accacgccc 
      aaagtgaaa ctcttcaaa cgcaaaacc atatcgggg 
      gattccctc gaactcaag aaatgtctc gagggaaaa 
      ttcgacata aaccgcgag ttctactcc gaccatgtc 
      tacaaagtc aagcagttt aaattcggg tcgcttgac 
      ccccccctc ccccaaccc tccacctcc acttcggac 
      tcggattcc gacgccgac ctcgatccg aaaagcatg 
      atcgagttg aatttgggc gcgactttc gtcgcccaa 
      ttggagagc ttgtttggt ggcgacgac ggtgcgttc 
      ccgcagggc taccaaccc gtggtgcaa atgccccta 
      aacctggcg cgccaaatt tacagcttc tacatcgag 
      tcagtgtgc cagcaaatg gacgctcag acgcacatt 
      ctcgaccgt ttggtcaaa gaagacgaa gagttggcg 
      cgcaagctg caaaagcag gaggaaagt cgcctggtg 
      ccccaacgt gagcccaac ttcaccgag ataatggac 
      gagcaagtc gccttgagt ttgcaccaa cgagccgta 
      gacgagtgg aagaacatg acggcggac aacttggcc 
      gccaagttg accaaacag aagttattt gccgcgttc 
      ccgaacata gatcagaac gcgttgctg gaaatatgg 
      cacgcttat gactacgat tataataaa acagtcgag 
      gatttgatc gcaaacgtg ggcgggtca ctagatgac 
      caaatcaag gagccgcca ttgagtgcc acaacgttg 
      caggaaatg aagcaggct gaggagaat tttgacgat 
      tttgagcaa aatgaagaa caaaaacct gcgacttac 
      taccgggag gaagcaaac aaacatttg aaacgacgg 
      gtcgaactc tacgagaga gctaaactg tacttccag 
      aagggcatg tttgaagtt gcaacgttc tattccgag 
      ttggctaag aaagagaca aagttgtac gacaaggcc 
      aatcacgtg gcagccgcg gctttcgcc gatgaacat 
      tcgaaaaga ctgcaaaag tttgatact ctcgacttg 
      cactatttc tacgtcaag gaggctatt cccaacttg 
      gatattttt atcgaccgt aacataaac ctgctcaaa 
      gggggggct aaaaacggt atcgacttg ttcgttatc 
      acaggcagg gggaaaaat agtgaaggg gggaggtgc 
      aaaatcaaa ccggcggtt ataacccgg ttgaagaaa 
      cgcaatatt gggttcgtt caaatgaat ccgggactt 
      ttgaaaata agggtcatg cgaaattcg ctaatgact 
      agtgatttg ccatag
    mRNA sequence
    • >TC011558 RA
      taactgcag cccatgttt atgttaagt tttcatcat 
      tattgaaga taacattat cagtgcaaa aaccattct 
      cgaatgaat tattgaaaa tgactaaat gagtcaccg 
      ttcgataat cacggtagt gtttattac gttgtacgt 
      atttttact cttattttt caaaagttc gttccacaa 
      taatcgaga aacccttaa cccccctca attccagcc 
      atgtcatcc cccagaaac cccaaacac cagcaagaa 
      ggcgtcgtg aaaagcctc cgcgatctt tttggtaac 
      attttccac atcgatatc atccgttca gtcgcacac 
      caatgcaat ttcgacatc gtaagatcc tccgacgtc 
      ctcatcgaa atgtccaac aacattgcc gaccaaccc 
      tcgaaaaaa aaacaaccg aaaaactcc ccgaaaaaa 
      accgacccg ccccgagtg atcaaatca aaaaaatcg 
      caagaattg gaacgtgtg attaacaat atcagacaa 
      ggctacaag gttttggtg attttgagg ggtttaccg 
      gggagtggc aagtcccaa ctagccgtt ggtatcact 
      gacgtcact ctgggcccg gggaataac agcccacat 
      atcctaagc actgacgat tattttatc caaaacggg 
      agatacgtt tttgaccca aacaggctc caggaagcc 
      cacgagtgg aaccatcgc agggccttc caggccatg 
      agtcggggg ctaagcccc gttataatc gacaacacc 
      aacacacaa atgtgggaa atgaagccc tacgccatg 
      atggccact gattacggc tacttgatt gaaattctc 
      gaacctgac acgtggtgg tgcttcaac gacaaggaa 
      ctagcgcgg agaaacagt cacggggtt ccccgaaac 
      aaaatcaag gacatgttg gaccgatac gacaaaaac 
      ataaccacg caaaaatta ctatgcgcc tatagtctc 
      acttacaag ttccaaaaa ccgccccaa ttccgccta 
      atcccccca attgtacga caaaatggc taccgcatt 
      caaaatggc gtcacgaaa tcgcgaagc acgccgatc 
      ttcgatcgg caaaagtcg atcgaaaac ggcgtcgag 
      gcgatcaat ttgatggat tttaacgac gtttcgaac 
      gaaactgag cagaatcag gccggcacg tgggacctc 
      aaccccgtc gacgccatc ttgatgtgc accgaagag 
      aaccaaacc gacgtctta caaccaaaa cccatcact 
      agcattgaa aacgcctgg ggtattgac gaaaaagcg 
      ctctattcc tgggacatt gtcactcca ctcgccgaa 
      gttggcacc cctgtcgac gccacaatt caaccccaa 
      gtcccaaaa cccgaaaca cgtgacgct tgcacgtgt 
      taccaggcc aacgattgg ctgaacgtt gctcacaaa 
      acagtggcg gcgcgtaac cgcgacatt aatgttggc 
      acacctgtg cgggtttta ccgccaccc aaaaaaaca 
      atgcttgat aagagctgc atcacggac gacgtgatg 
      gacgaggag gtgagcgaa gaggagcga attaaagac 
      ttgatggat ttgttccca aaactaccg aaatcgtcg 
      ctttgtgaa ctgtttaat aaatgtcac aagaacttt 
      cactggact gttgatctt ctcctcgaa gacagtaac 
      cttgaaatg gccgaaacc gatgacgtc accacgccc 
      aaagtgaaa ctcttcaaa cgcaaaacc atatcgggg 
      gattccctc gaactcaag aaatgtctc gagggaaaa 
      ttcgacata aaccgcgag ttctactcc gaccatgtc 
      tacaaagtc aagcagttt aaattcggg tcgcttgac 
      ccccccctc ccccaaccc tccacctcc acttcggac 
      tcggattcc gacgccgac ctcgatccg aaaagcatg 
      atcgagttg aatttgggc gcgactttc gtcgcccaa 
      ttggagagc ttgtttggt ggcgacgac ggtgcgttc 
      ccgcagggc taccaaccc gtggtgcaa atgccccta 
      aacctggcg cgccaaatt tacagcttc tacatcgag 
      tcagtgtgc cagcaaatg gacgctcag acgcacatt 
      ctcgaccgt ttggtcaaa gaagacgaa gagttggcg 
      cgcaagctg caaaagcag gaggaaagt cgcctggtg 
      ccccaacgt gagcccaac ttcaccgag ataatggac 
      gagcaagtc gccttgagt ttgcaccaa cgagccgta 
      gacgagtgg aagaacatg acggcggac aacttggcc 
      gccaagttg accaaacag aagttattt gccgcgttc 
      ccgaacata gatcagaac gcgttgctg gaaatatgg 
      cacgcttat gactacgat tataataaa acagtcgag 
      gatttgatc gcaaacgtg ggcgggtca ctagatgac 
      caaatcaag gagccgcca ttgagtgcc acaacgttg 
      caggaaatg aagcaggct gaggagaat tttgacgat 
      tttgagcaa aatgaagaa caaaaacct gcgacttac 
      taccgggag gaagcaaac aaacatttg aaacgacgg 
      gtcgaactc tacgagaga gctaaactg tacttccag 
      aagggcatg tttgaagtt gcaacgttc tattccgag 
      ttggctaag aaagagaca aagttgtac gacaaggcc 
      aatcacgtg gcagccgcg gctttcgcc gatgaacat 
      tcgaaaaga ctgcaaaag tttgatact ctcgacttg 
      cactatttc tacgtcaag gaggctatt cccaacttg 
      gatattttt atcgaccgt aacataaac ctgctcaaa 
      gggggggct aaaaacggt atcgacttg ttcgttatc 
      acaggcagg gggaaaaat agtgaaggg gggaggtgc 
      aaaatcaaa ccggcggtt ataacccgg ttgaagaaa 
      cgcaatatt gggttcgtt caaatgaat ccgggactt 
      ttgaaaata agggtcatg cgaaattcg ctaatgact 
      agtgatttg ccatagtga taaatttaa gttggttgc 
      agtgtaacc aagctatga cccgatcaa gggtttgag 
      agtacaaat tgctttggg acgttagca ttttggttt 
      tattttttc aggcgttgg taccgccga tatttaaca 
      tgttactgt ttttggttg gaataaatt tcgcattgt 
      gactaattt taaaacatt tttattgtt attatgact 
      acaaatatg agtaccact attattgtt atgaatgca 
      catcatttt tatacagtt ccaaaatat atattttac 
      taatta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY