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Gene information
General information
TC011550 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 3797200..3799515
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011550
    iBeetle screen iB_05054

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: 3 - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection



    Pupal morphology
    pronotum bristle size shortened - Number of animals showing the phenotype: 9

    abdomen gin-trap setae size shortened - Number of animals showing the phenotype: 9

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011550 RA
      MPSKKKKYN ARFPAGRIK KIMQTDEEV GKVAQAVPV 
      IISRTLELF VESLLTKSM QITQSRNAK TLTPSHMKQ 
      CILSESRFD FLKDLVKNI PDASAQEDN KNNLMFEAQ 
      QLQEAESKQ EPAAAVEAS KPEVTANFY TEQSTSTAR 
      TPVIQYGPK VAQVETKKF NFSVNNLIE PAKEGPKVR 
      ISVAEPVPV PPLVPTRGP ANSVQENTP PVNCDNIPP 
      LIPISHHYY NQNTEDTLC IDEDYDN
    CDS sequence
    • >TC011550 RA
      atgccttca aaaaagaaa aaatacaat gccagattt 
      ccagcaggc cgtattaaa aaaataatg cagactgat 
      gaagaggtt ggaaaagtg gcacaagct gtaccagtt 
      ataatctcc cgaacgctg gagttgttt gtggagtcg 
      ctgctgacg aagtcaatg caaataacg cagtcccgc 
      aacgccaag actttaact ccgtcccac atgaagcaa 
      tgtatatta tccgagagt cgtttcgac tttttaaaa 
      gacttagtg aaaaatatc cctgatgca agtgcacaa 
      gaagataac aaaaacaac ctgatgttc gaagcgcaa 
      cagctgcag gaggccgag tcaaaacag gagcctgcg 
      gcagcagtg gaggcttcg aagccggaa gtaacggcg 
      aatttttac acagagcag agtacgtcc acggctaga 
      acaccagtt atccagtat ggacctaaa gtagcgcaa 
      gtagagacg aaaaagttc aatttctca gttaataat 
      ttgatcgag ccggcgaag gaaggaccc aaagtgcgc 
      atttcggtg gcggaaccc gtacctgtg cccccttta 
      gtaccaaca agggggccc gctaatagt gtccaagaa 
      aatacgccg ccagtcaac tgcgacaat atcccgcct 
      cttatacct atttcacac cattattac aatcagaac 
      actgaagac actctgtgt attgatgaa gattatgat 
      aattag
    mRNA sequence
    • >TC011550 RA
      gtgattgtt gacaaattg aaacgaaat caaagattt 
      ttagatatg ccttcaaaa aagaaaaaa tacaatgcc 
      agatttcca gcaggccgt attaaaaaa ataatgcag 
      actgatgaa gaggttgga aaagtggca caagctgta 
      ccagttata atctcccga acgctggag ttgtttgtg 
      gagtcgctg ctgacgaag tcaatgcaa ataacgcag 
      tcccgcaac gccaagact ttaactccg tcccacatg 
      aagcaatgt atattatcc gagagtcgt ttcgacttt 
      ttaaaagac ttagtgaaa aatatccct gatgcaagt 
      gcacaagaa gataacaaa aacaacctg atgttcgaa 
      gcgcaacag ctgcaggag gccgagtca aaacaggag 
      cctgcggca gcagtggag gcttcgaag ccggaagta 
      acggcgaat ttttacaca gagcagagt acgtccacg 
      gctagaaca ccagttatc cagtatgga cctaaagta 
      gcgcaagta gagacgaaa aagttcaat ttctcagtt 
      aataatttg atcgagccg gcgaaggaa ggacccaaa 
      gtgcgcatt tcggtggcg gaacccgta cctgtgccc 
      cctttagta ccaacaagg gggcccgct aatagtgtc 
      caagaaaat acgccgcca gtcaactgc gacaatatc 
      ccgcctctt atacctatt tcacaccat tattacaat 
      cagaacact gaagacact ctgtgtatt gatgaagat 
      tatgataat tagattgtt ctatattta tcaccgatt 
      tatcagttc atttgtatc aagtaaacg taactacct 
      acctgacga cttttccga tgacctaac agtgaaata 
      tacagttgt tattaaaac gttttgtat tgggtaaaa 
      aaatacatt agtacatta tgtacaatt ttaaaaata 
      ttaaagtat tataatcaa taccttata tacatacaa 
      ctacttttt taactcctc tgaaggacc tcatgagcc 
      tgccaatcg ttactttta tttatataa gttagaaat 
      taaataata aagtcatta aattattac atcttgttt 
      tttacatta ttatgggca caagttctc atcacggcg 
      catataatt tgaactatg gaaaataat acaaggcga 
      cttactctt aatattgca aattgtaag tgggcgatt 
      agttacact gttaaaact taaataagg taactacaa 
      tttacattt gttacataa acctattcg aaagaataa 
      ataaaggaa ataagttaa ttacagact tttcaattc 
      tattttaac aagtgttag ataatacaa acgcactct 
      tagactatg aacgactta tcagtctgg gaaaaccga 
      accgaagtt gaatttttt aatttgtgt tttttttta 
      cttccatcg ttcgacatg tccagtgta gacgtattt 
      tgtccgata tattgacac tcgggggaa gaaaattaa 
      attccacaa cttgtccaa agggtcatt gaattttta 
      atgctgata aacattgat ttattaaca tatgcccta 
      acaaaaata aggttttat catccaaaa caaataatt 
      catgggtgc aaaataaga aatgcaaat atgtaacgt 
      tgagtgttt gttcaagat aacgtttca ggaatttcc 
      tagtgattc atacttttt tcctaaata tgtacataa 
      aaacaaacg agttttttg ctcaataaa tgcaaatgg 
      aaaatttgg aaatacaat atgtcgctg gtaaaacta 
      cggatatat ctcgggact ttttcaatt gacactctg 
      gcaacatgg caatttcgt acacaaata cctaatttt 
      ggtctaaaa gatgaattt gcgttattt tgtaaatcg 
      tctttccaa gaaaacaaa caaaactag atttttttt 
      taaattaat tttattgta ttagcagct gtaaactga 
      ataaaagtg aagcaataa attggaatt atttaatac 
      tctcttgtc tattcgact aaaattttt ggccgaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY