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Gene information
General information
TC011533 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 3639893..3645097
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC011533
      iBeetle screen iB_05050

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC011533 RA
        MGCASSSPL VESGKNLVE TAKNQAGDV 
        VNKGEKTLQ DVGDTVKEK ISTEMEHVK 
        EAMQSAMNT AENSIGSVM GKMGNSINS 
        ASEEVKDTV NNMEDEAEE ALHVASDSA 
        AEAVSEAET VLASEAENA KEMLTSTAN 
        DAFDKFDSG EKAIEKKLH EMQEKVEDG 
        LEIEDMEQH YDMLGEGVK NVEKKVEEI 
        VAKLPTPAA SPTKEPQKE E
      CDS sequence
      • >TC011533 RA
        atgggttgt gcaagtagc agtccactt gtggaaagt 
        ggaaagaac ttagtggag actgcaaaa aatcaagcc 
        ggtgatgtt gtgaacaaa ggggaaaag acactgcaa 
        gatgtagga gataccgta aaagaaaaa attagcact 
        gaaatggag cacgtgaag gaagcaatg cagtcagcc 
        atgaatacg gccgaaaac agcataggc tcagtgatg 
        ggcaaaatg ggcaacagc ataaattca gcaagtgag 
        gaagtaaaa gacactgta aacaacatg gaagacgag 
        gctgaggaa gcactacat gttgcctcg gattcggca 
        gcggaagcg gtttctgaa gctgaaacc gtcctagcg 
        tcagaagcc gaaaacgcg aaagaaatg ttaacgtca 
        acggcaaac gatgcgttt gataaattt gattccggt 
        gagaaagcg atagagaaa aaattgcac gaaatgcag 
        gagaaagtc gaagacggg ttggagatt gaagacatg 
        gagcagcat tacgatatg ttaggagaa ggtgtaaaa 
        aatgtagaa aagaaagtt gaggaaatt gtagcaaag 
        ttacccact cctgcagct tcgccgacc aaggaaccg 
        cagaaggag gagtaa
      mRNA sequence
      • >TC011533 RA
        tcaaaaata aacataaaa atataattt aaccattta 
        ccgagacct tttcaatta ggtatcata atgaaatta 
        agcgaaaac gcaaaatcg ttttcttta tttctacta 
        tttacaatg attccgtga cattcaaaa aatccaagc 
        tattgcagg aaacctttt aaaacactc atttagaaa 
        gaagagttt ttaaacttt gttaaaata gcatttttt 
        taataattt caaatattt tgtctccga attcgttct 
        gtgtttaga atttttttt ttggtctat ttactgttg 
        tatctgtct ttatatttg atctctaaa ctcagaagc 
        aatattcgt gcctctaat tggaagtta ctaatcaca 
        ttgtgcaaa agcagttaa gtaattttt agtttcgaa 
        aaagagtgt attctagtt cacacactt ttagcacac 
        cttgccctt atctaagaa aatataact gtggcctca 
        ataaaatat agtccctga cacgcaatc gacaagata 
        taatcttaa aaattgttt taaacacag ctggaaacg 
        ttatactca atgttttgt ataagttca tactcgaaa 
        ttattataa attggacac ttaactcac cataaaaca 
        atttacttt aattcaatt tcaggttcg tagttctat 
        acaaaatta tagttttta gtccaaatc ctgtcttga 
        ctaatgtat ccagtcatt attctgcaa tcaatgtca 
        taaaaagag aaacattct ataaattag atttgttta 
        aaatatatt tcctcaaca ctgaatgtt agaagcaat 
        attagtttc caacacaca aaagtgcac cttacagtc 
        ggcaacatt tacagcaaa aaacccatt atctgtaat 
        gtttttctt agataagta actagaaga tggatgtgg 
        atagattgt gtgtattta acaaatttt taaaggcat 
        aaaacaggc ataaaactt attaaaagt cgatccgaa 
        tttttaaaa ccgacagtt ttagaatcg tttgggatt 
        tatttttca agtcacaca cgctaaaat ttacgataa 
        aattaaaat caaaaatat aaattcacc gtttaatgg 
        gtacatcaa aaattaata cgcatatca cgttttatg 
        tagcaagtt ttacactaa ttagtttag attagtttt 
        gaatcaaat gcctaaatg ttcatcggc actaaagat 
        gtaccatat caatatctt ctgtaatat ttaaatcag 
        aatttgata cgttacaat gttacacag ttgttacca 
        acttgttgt aaaatagct agctacgta ttcagtaaa 
        tagcagaac gttaataaa tatctgcac tctaaatta 
        aggtgccct aaatatttt tatatgtgt atatttaaa 
        actccacgg caagggtgc cagacgtaa tgaccgctg 
        gtcagtttt accgacttg gtcgttaca aacagtagt 
        aaaatgggt tgtgcaagt agcagtcca cttgtggaa 
        agtggaaag aacttagtg gagactgca aaaaatcaa 
        gccggtgat gttgtgaac aaaggggaa aagacactg 
        caagatgta ggagatacc gtaaaagaa aaaattagc 
        actgaaatg gagcacgtg aaggaagca atgcagtca 
        gccatgaat acggccgaa aacagcata ggctcagtg 
        atgggcaaa atgggcaac agcataaat tcagcaagt 
        gaggaagta aaagacact gtaaacaac atggaagac 
        gaggctgag gaagcacta catgttgcc tcggattcg 
        gcagcggaa gcggtttct gaagctgaa accgtccta 
        gcgtcagaa gccgaaaac gcgaaagaa atgttaacg 
        tcaacggca aacgatgcg tttgataaa tttgattcc 
        ggtgagaaa gcgatagag aaaaaattg cacgaaatg 
        caggagaaa gtcgaagac gggttggag attgaagac 
        atggagcag cattacgat atgttagga gaaggtgta 
        aaaaatgta gaaaagaaa gttgaggaa attgtagca 
        aagttaccc actcctgca gcttcgccg accaaggaa 
        ccgcagaag gaggagtaa gtggtctga tgtgagtgt 
        taaccaaaa tcgtgctgg atttgctct gtacaaagt 
        gatgattgg tcttggagt gaatagcac gagcattat 
        acaaagata ttgtaaata gagatagct ttttggacc 
        cctagctga tgataaatt ttctatttt actttacat 
        acaatttgg aaaataaat cttattaaa gacaggatg 
        catctcttt tatttttcc attatcatt gcttcgcaa 
        caattgtgc caaagctcg actataaat aggcccgat 
        tctaggtac gatattagt tgatttgca aatttattc 
        aacgcatac agacaggtg gtaaattga ctttctgtt 
        atggaa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY