Status
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Gene information
General information
TC011532 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 3633253..3639661
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011532
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05049

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011532 RA
      MHQRAFLGG GWARGGASE VVRRAHPAS GAQWNVQVV 
      RGKVNGKCL WNACKALSL GLLLMVLGA AMATIGYYA 
      DHLSIAQEV RGNHTVRVK NESRGFHLN NLSYAGPII 
      MGVGGFIVV AACVMTFEA RDSAAKVVP ARLKLSTNG 
      PPSGSYSGY AGHTHGGYS GHGSLRTSG SQTGSVHQQ 
      ASHSHARSG ATDRRALTE SFLNFSRGL SLKSQRTSS 
      LKIPQGSIN KSPSAPNLV LEHPVDSHT RSSPVFSRY 
      GTEKANSKS SRRTFANCA LLNPSLLHR HALSVDETA 
      ANYRLSHES LQGSGSQGS LALDLHLEC PVTLRIRDK 
      RRNPLIRQR RVDEDERQG YDDSSRRSS HSCSPRIPM 
      HIKDMKSGG SGGSTHQLP VYVPYTISR RRSSNASDS 
      THRSRAKRR EYPHSRGKL ERAISSDSR LMGAIPRTH 
      RHYSPTQGS SIEQEPGGS SSDTDIRKA HVVHFCH
    CDS sequence
    • >TC011532 RA
      atgcatcaa agagccttt ttaggcggc ggttgggct 
      cgaggcggt gcatctgaa gtagtccgg agggcacat 
      cctgcatcc ggagcacaa tggaacgtt caagtggtc 
      agaggtaaa gtgaacggg aaatgtctg tggaatgct 
      tgcaaggct ttgagtctt ggcttactt ttaatggtt 
      ttaggagca gcgatggcc accataggt tattatgca 
      gaccatctt tctattgct caagaagta agaggaaat 
      cacacagtg cgagttaaa aacgaatcg agaggtttt 
      catttaaat aacctctct tatgcgggg cctataatc 
      atgggagtg ggaggattt atcgtagtc gctgcctgc 
      gtaatgaca ttcgaggca cgagattct gcagcaaaa 
      gtagttcct gcacgcctc aaactgagc acaaatgga 
      ccaccaagt ggttcctac agcggttac gcaggccac 
      actcacggt ggctacagc ggccacggt tcccttcga 
      acctccggc tcacaaact ggaagtgtt catcaacag 
      gcctcccac agccatgca agaagcggc gccactgac 
      agacgcgcc cttactgaa tcattctta aatttttct 
      agaggcctt tccctaaaa agccagcgc accagctcg 
      ctcaaaatt ccacagggt tctataaac aaaagtcct 
      tcggctccc aacctagtc ctggagcat cctgtagat 
      agccacact cgctcttcg cccgtcttc agtcgctac 
      gggactgaa aaagcgaat tcgaagagt tctcggcga 
      acattcgcc aactgtgct ttgttaaac ccgagtctt 
      ttgcacaga catgcttta tcggtggac gaaaccgct 
      gcaaattat agattaagt cacgagtcc ctccaagga 
      agcggtagt caaggctcc ctagcactg gacctccac 
      ctagagtgt cctgtcaca ttgcgaatt cgcgacaaa 
      cgcagaaac cctctgatc aggcaaaga cgggttgat 
      gaagacgag cgccagggt tatgacgat agctcccgg 
      aggagctcg cattcgtgt tcccccagg attccgatg 
      cacatcaaa gatatgaaa agtggtggg agcggtggt 
      tcgactcat cagctgccg gtttatgtg ccgtacacc 
      atttcccgg agacggagc tccaacgcg tcggattcc 
      acacatcga tccagggcc aagagacgg gaatatccc 
      cattcgcgc ggaaaatta gaaagagca ataagttca 
      gattcaaga ctgatgggc gccatcccg agaactcat 
      cggcattac tctccgact caaggttcg tcaattgaa 
      caggaacca ggagggtct agttctgac actgatatt 
      cgtaaagct catgttgtt cacttttgt cactga
    mRNA sequence
    • >TC011532 RA
      cctgtgttg accatgggg ccgtcgcat cacggctgc 
      attgatcac ctcgtgcta cctgcactg actgtcgag 
      tgcctggag atgctgttc ctgttacca tgacagcgt 
      cgcggcgca agcgatgta ccagatcgg cgtcgctat 
      tgtggtcca ggtggctga tgcggaccc cgaacaacg 
      gcagacgct cgcagtgat gtggccctt ccggggcga 
      ggtctcgtg cgcggcata atacggcca aattgcctc 
      ataaatact ttgcatgcg gtgattgtg ccggagaat 
      tgcttcgac cgatgaatg catgtcttt caaggcaga 
      gtttgttta gcctggagg gacggtcga taaggcggc 
      ggactcgcg catgattgt acctgttgc ccgtggtcc 
      acggggatg ctgagcgga taccgacct cattatcgc 
      aacatacac agcgattag gataattag tgcgggctg 
      tgatgtggg acaagacac gctaacaaa tcgcacgag 
      aaactagca attcacaca ccacataca ctgctcagg 
      taaatggtg ttgtcctcc tagcatatg ttacccctc 
      cttgacaat cacttacca aattgcacc cctaaataa 
      agaagagcg aaaacagtt atcttgttt actcaatcc 
      cgcctagaa cacgaaatc atgcgcagg gtttaagat 
      aatgattat aaaagcttg agagagaat attcatgaa 
      agagtaagt ggcagattt tatgaagtg caattcaag 
      agtttgcat agcgacaga tgaatcgga ggctattcc 
      tatttccgt taacaaaac ttcaaaaag ctcctaaaa 
      taaatattt ggataaata tacacatgc ggacgtaaa 
      aataaatag cgtctttcc gccatcagt ggaaaacca 
      gaacatgca tcaaagagc ctttttagg cggcggttg 
      ggctcgagg cggtgcatc tgaagtagt ccggagggc 
      acatcctgc atccggagc acaatggaa cgttcaagt 
      ggtcagagg taaagtgaa cgggaaatg tctgtggaa 
      tgcttgcaa ggctttgag tcttggctt acttttaat 
      ggttttagg agcagcgat ggccaccat aggttatta 
      tgcagacca tctttctat tgctcaaga agtaagagg 
      aaatcacac agtgcgagt taaaaacga atcgagagg 
      ttttcattt aaataacct ctcttatgc ggggcctat 
      aatcatggg agtgggagg atttatcgt agtcgctgc 
      ctgcgtaat gacattcga ggcacgaga ttctgcagc 
      aaaagtagt tcctgcacg cctcaaact gagcacaaa 
      tggaccacc aagtggttc ctacagcgg ttacgcagg 
      ccacactca cggtggcta cagcggcca cggttccct 
      tcgaacctc cggctcaca aactggaag tgttcatca 
      acaggcctc ccacagcca tgcaagaag cggcgccac 
      tgacagacg cgcccttac tgaatcatt cttaaattt 
      ttctagagg cctttccct aaaaagcca gcgcaccag 
      ctcgctcaa aattccaca gggttctat aaacaaaag 
      tccttcggc tcccaacct agtcctgga gcatcctgt 
      agatagcca cactcgctc ttcgcccgt cttcagtcg 
      ctacgggac tgaaaaagc gaattcgaa gagttctcg 
      gcgaacatt cgccaactg tgctttgtt aaacccgag 
      tcttttgca cagacatgc tttatcggt ggacgaaac 
      cgctgcaaa ttatagatt aagtcacga gtccctcca 
      aggaagcgg tagtcaagg ctccctagc actggacct 
      ccacctaga gtgtcctgt cacattgcg aattcgcga 
      caaacgcag aaaccctct gatcaggca aagacgggt 
      tgatgaaga cgagcgcca gggttatga cgatagctc 
      ccggaggag ctcgcattc gtgttcccc caggattcc 
      gatgcacat caaagatat gaaaagtgg tgggagcgg 
      tggttcgac tcatcagct gccggttta tgtgccgta 
      caccatttc ccggagacg gagctccaa cgcgtcgga 
      ttccacaca tcgatccag ggccaagag acgggaata 
      tccccattc gcgcggaaa attagaaag agcaataag 
      ttcagattc aagactgat gggcgccat cccgagaac 
      tcatcggca ttactctcc gactcaagg ttcgtcaat 
      tgaacagga accaggagg gtctagttc tgacactga 
      tattcgtaa agctcatgt tgttcactt ttgtcactg 
      aatctttac cgttaccga gctattatc tgaatacga 
      agacttgaa atacgtacg aaaaatata cgaagcaca 
      gtcgttcaa ccactgaat ctactataa tatattttt 
      tataataga acgaaatat ttcgcacgt atgactgaa 
      taaatcaac attaaacat aaaaaattg tcgtactaa 
      atcaaagtt tttgattag aataaagcc gtccgctgg 
      tagttgcgg tctgaagaa tttagagac aataaaatc 
      ctaaacacg agtaaaaat acaaacact gtttaattt 
      ttttttcta acattacct aactgaaga aatttacat 
      ctacaagta tttgataaa tgttacaat tgacgaata 
      aaattaatc tactcttgc ttattcggt ctaataata 
      ttaaaagac tatacattt ttttaaatg agtccctat 
      ccttcatat acacgaact ataaataca actaatgtt 
      acactaacc aattaacat tttcaaaaa ttctgtgag 
      atacgtggt ttgatcaat aagtacggc aaatggttg 
      gttgcatta tccatgaac gttattttg tcatcaaac 
      taccaagca gtaaagcaa tcggtttac cggtttact 
      gtctgcaat caaaaatca aattttttt ttctagtgt 
      gaaacagca tgaaatatt cctgcttgg tggagaata 
      gaaaaatgt aggaaacaa tttttttct tttcataaa 
      gaattaagg ctgttttaa aggtgccag cagattatt 
      aaaatgtgt ggcaagtct caaaaagac tccgcagaa 
      cattaaatg taaattctt taataaatt ttgtgagtt 
      gaccattca ccgtattta ttgaacaga acaatgtag 
      caaaatatt ttacctaat agatactgt atctcactt 
      ttttcagtt tttcagaac ataatgtat aaagaattt 
      atgttgaaa attcagtaa ccactatct cattcaagg 
      tttagaaaa gttttgttt atttttctc taatgctct 
      tagctacat attattttt tgactagtc ctaaagtca 
      gcaaataaa cacaacaca ttgagtata tcgtaaaaa 
      taaattatt tgctgaaag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY