Status
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Gene information
General information
TC011526 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 3703746..3724920
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011526
    iBeetle screen iB_05048

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature fluorescence not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    legs tibiotarsus size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection



    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    Pupal morphology
    wings size shortened - Number of animals showing the phenotype: 6

    wings shape rounded - Number of animals showing the phenotype: 6
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011526 RA
      MKKSLSNTK YEKLSQNSC KDLDENLVH PTSNSEQAG 
      NPAMWLSAG EATSQSYEE VADRLHVDV RHGLSWQEA 
      TRRRQIAGY NEFSVKDED PPWKKYLEQ FKNPLILLL 
      LASALVSVC MKQFDDAIS ITVAIVIVV TVAFVQEYR 
      SEKSLQELT KLVPPSCHC LREGSPDTF LARELVPGD 
      IVYLNVGDR VPADLRLFE SVDLMIDES SFTGETEPA 
      RKNVAAVLR PNGAHSSNT NIAFMGTLV RCGNGKGIV 
      VSTGSNSEF GSVFKMMLE EEAPKTPLQ KSMDSLGAQ 
      LSIYSFGII GLIMLVGWL QGKAIMEMF TIGVSLAVA 
      AIPEGLPIV VTVTLALGV MRMAKRNAI VKKLPTVET 
      LGCVNVICS DKTGTITRN EMTVTVLVT ADGYIAELT 
      GAGYNDHGQ VLLHKCDSS DRARDSVYK LLEVGAVCN 
      NAVIHNETL LGQPTEGAL IAAAMKHGM YNVADRYVR 
      VQEYPFTSE QKMMAVKCI SKYDNDKVE IFFVKGAIE 
      KILPKCTKF SWNGGTQSL TSKKEQDFI AEAHEIGRK 
      GLRVIAMAR GPTLQDLTY MGIVGICDP PRPLVREAI 
      TTLSQSGVQ VKMVTGDAE DTAVAIAQT IGLETLPSQ 
      VLSGEQLDT FTETDLDAA IPHATVFYR VSPKNKLAI 
      VKSLQRTGH IVGMTGDGV NDGVALKKA DIGIAMGKN 
      GTDVCKEAA DMILVDDDF YTIIAAIEE GKSIFYNIR 
      NFVRFQLST SIAALSLIA LATLMGIPN PLNAMQILW 
      INIIMDGPP AQSLGVEPV EKDVVKQKP RNTKEPMIT 
      KKLIGNVLL SALFIIAGT LWVFKKEMS SEGITARDT 
      TMTFTCFVF FDMWNALSC RSQTKSVFQ IGLFSNKMF 
      LVAVTLSVI GQLLVVYFP PLQRIFQTE ALTLWDMVF 
      LTTLTSSVW VFSEIKKLI ERFLEQRGK GKRSPLEYV
    CDS sequence
    • >TC011526 RA
      atgaaaaaa tcgttatca aacacgaaa tatgaaaaa 
      ctgtcgcag aattcttgc aaagatcta gatgaaaat 
      ttggtgcac ccaacatct aattcggaa caggctggt 
      aatcctgcg atgtggctc tcagcaggc gaggcgaca 
      agtcaaagc tatgaagaa gttgccgat agactgcac 
      gtagacgtg agacatggc ttaagttgg caggaagcc 
      acacgtaga cgacaaata gctggttac aatgaattt 
      tcagtcaaa gacgaagat ccgccttgg aaaaaatac 
      ctcgaacaa tttaaaaac cctctcatt ttacttctt 
      ctagcctcg gcacttgtc tcagtgtgc atgaaacaa 
      ttcgacgat gcgatcagt attacagta gccatagtc 
      atcgtcgta accgttgct tttgttcaa gaataccga 
      tcggaaaaa tcgctgcaa gaactcacc aagctcgtg 
      ccaccgtct tgtcattgt ttgagggaa ggttcgcct 
      gatactttt ctagcccgc gagttagtg ccaggtgat 
      atcgtttat ttgaatgtc ggcgatcga gtgcccgca 
      gatttgcgt ttatttgag tcagttgat ttgatgatc 
      gatgagagt agtttcact ggcgagact gaacctgca 
      aggaaaaat gtcgctgcg gttttgcgc cccaatggg 
      gcgcattct tccaacact aacattgca tttatgggg 
      acgcttgtc cgctgcggg aacggcaaa gggattgtt 
      gttagtacg ggctctaac agcgagttc gggtcggtg 
      tttaagatg atgttggag gaagaggcg ccgaaaacg 
      cctctgcag aagtccatg gacagtttg ggtgctcaa 
      ttgtcgatt tactccttc ggcattata ggtttaatt 
      atgttggtc gggtggttg caaggcaag gcaatcatg 
      gagatgttt acgatcggt gtgagttta gcggtcgct 
      gcaattcca gaaggatta ccaattgtc gtgacggta 
      acactagcc ttgggcgta atgcgaatg gcaaaaaga 
      aacgccatc gtaaagaaa ctaccaact gtcgaaaca 
      ctgggttgc gtcaatgtg atatgttct gacaaaaca 
      ggcactata acacgtaac gaaatgacc gtcacagtt 
      ttggtaaca gccgatggt tacattgcc gaattaaca 
      ggcgccggt tacaacgac catggccaa gttctatta 
      cacaaatgt gattcatca gacagggct agagatagt 
      gtatataaa ttattagag gtaggggct gtttgcaat 
      aacgcagtg atccataac gaaacttta ctcggacaa 
      ccaacagag ggcgctcta atcgccgct gcgatgaaa 
      cacggcatg tataacgta gcggaccgt tacgtccga 
      gtccaagaa tatccgttc acttctgag caaaaaatg 
      atggccgtt aaatgcata tctaagtat gataacgac 
      aaagtcgag atttttttc gtcaaaggt gccatcgaa 
      aaaatacta ccaaaatgt acgaagttc agttggaac 
      ggaggaacc caaagtctt acaagcaaa aaagagcaa 
      gattttatc gcagaggct catgaaatc gggcggaag 
      ggcttaaga gtgattgca atggcccgt ggaccgact 
      ttacaagac ctgacctac atgggcatt gtgggtatt 
      tgtgaccca ccacgtcct ctggtcagg gaagccata 
      actacgtta tcacagtct ggggtgcag gtcaaaatg 
      gtaactgga gatgctgaa gatacagct gtggctata 
      gctcagact attggcttg gaaactttg ccctctcag 
      gtgttgtcg ggcgaacag ttggatacg tttactgaa 
      accgatttg gatgcggca atacctcat gctacagtt 
      ttttatcga gtttcaccg aaaaataaa ttagcgatt 
      gttaagtcg ttgcaaagg actgggcat atcgttggg 
      atgactggt gatggagtg aatgatggt gtggcgctt 
      aaaaaagcc gatattggg attgccatg ggcaaaaat 
      gggacggat gtgtgtaaa gaagccgca gatatgata 
      ctcgttgat gatgatttt tacacaatt attgcagca 
      attgaagaa gggaagagc atattttac aacatccga 
      aactttgtc agattccaa ttatctacc tctattgcg 
      gctttgtct ctaatcgca ctagcgact ttgatggga 
      attccgaat ccgttaaac gccatgcaa attttgtgg 
      atcaacatt ataatggac ggtcctccg gcccaaagt 
      ttgggcgta gaaccggtc gaaaaagac gtggtaaaa 
      caaaaacct cgaaatact aaagaacca atgatcact 
      aaaaaacta atcggaaac gttctactt tccgcctta 
      tttataata gctggaacg ttatgggtg ttcaaaaaa 
      gagatgagt tcggaaggt attacagca cgcgataca 
      acaatgacg ttcacttgt ttcgtattt ttcgacatg 
      tggaacgct ttatcctgt cggtcgcaa accaagagc 
      gttttccag attggtttg ttctccaat aaaatgttt 
      ctggtggct gtcaccctc tccgtgatt ggacaactt 
      ctcgttgtt tactttcca cctttgcag cgaattttc 
      caaaccgaa gctctcaca ttatgggac atggtattt 
      ttaacaaca ctaacgtct tcggtttgg gtcttttcg 
      gagataaag aagctgatc gagcggttt ctcgaacag 
      cgagggaaa ggcaagcga tcgcctttg gagtatgta 
      tga
    mRNA sequence
    • >TC011526 RA
      cagttgtca gtttttgac agctcctgt cgatatgag 
      tagtgtgtg tgcgatcaa actctttcg attcgtgtc 
      aatatcccc tttataaat taaaatgga acaaattta 
      tttaaacag tgcaacata acgcttgtt acaaagtta 
      ctttccttg tcgaagcat gaaaaaatc gttatcaaa 
      cacgaaata tgaaaaact gtcgcagaa ttcttgcaa 
      agatctaga tgaaaattt ggtgcaccc aacatctaa 
      ttcggaaca ggctggtaa tcctgcgat gtggctctc 
      agcaggcga ggcgacaag tcaaagcta tgaagaagt 
      tgccgatag actgcacgt agacgtgag acatggctt 
      aagttggca ggaagccac acgtagacg acaaatagc 
      tggttacaa tgaattttc agtcaaaga cgaagatcc 
      gccttggaa aaaatacct cgaacaatt taaaaaccc 
      tctcatttt acttcttct agcctcggc acttgtctc 
      agtgtgcat gaaacaatt cgacgatgc gatcagtat 
      tacagtagc catagtcat cgtcgtaac cgttgcttt 
      tgttcaaga ataccgatc ggaaaaatc gctgcaaga 
      actcaccaa gctcgtgcc accgtcttg tcattgttt 
      gagggaagg ttcgcctga tacttttct agcccgcga 
      gttagtgcc aggtgatat cgtttattt gaatgtcgg 
      cgatcgagt gcccgcaga tttgcgttt atttgagtc 
      agttgattt gatgatcga tgagagtag tttcactgg 
      cgagactga acctgcaag gaaaaatgt cgctgcggt 
      tttgcgccc caatggggc gcattcttc caacactaa 
      cattgcatt tatggggac gcttgtccg ctgcgggaa 
      cggcaaagg gattgttgt tagtacggg ctctaacag 
      cgagttcgg gtcggtgtt taagatgat gttggagga 
      agaggcgcc gaaaacgcc tctgcagaa gtccatgga 
      cagtttggg tgctcaatt gtcgattta ctccttcgg 
      cattatagg tttaattat gttggtcgg gtggttgca 
      aggcaaggc aatcatgga gatgtttac gatcggtgt 
      gagtttagc ggtcgctgc aattccaga aggattacc 
      aattgtcgt gacggtaac actagcctt gggcgtaat 
      gcgaatggc aaaaagaaa cgccatcgt aaagaaact 
      accaactgt cgaaacact gggttgcgt caatgtgat 
      atgttctga caaaacagg cactataac acgtaacga 
      aatgaccgt cacagtttt ggtaacagc cgatggtta 
      cattgccga attaacagg cgccggtta caacgacca 
      tggccaagt tctattaca caaatgtga ttcatcaga 
      cagggctag agatagtgt atataaatt attagaggt 
      aggggctgt ttgcaataa cgcagtgat ccataacga 
      aactttact cggacaacc aacagaggg cgctctaat 
      cgccgctgc gatgaaaca cggcatgta taacgtagc 
      ggaccgtta cgtccgagt ccaagaata tccgttcac 
      ttctgagca aaaaatgat ggccgttaa atgcatatc 
      taagtatga taacgacaa agtcgagat ttttttcgt 
      caaaggtgc catcgaaaa aatactacc aaaatgtac 
      gaagttcag ttggaacgg aggaaccca aagtcttac 
      aagcaaaaa agagcaaga ttttatcgc agaggctca 
      tgaaatcgg gcggaaggg cttaagagt gattgcaat 
      ggcccgtgg accgacttt acaagacct gacctacat 
      gggcattgt gggtatttg tgacccacc acgtcctct 
      ggtcaggga agccataac tacgttatc acagtctgg 
      ggtgcaggt caaaatggt aactggaga tgctgaaga 
      tacagctgt ggctatagc tcagactat tggcttgga 
      aactttgcc ctctcaggt gttgtcggg cgaacagtt 
      ggatacgtt tactgaaac cgatttgga tgcggcaat 
      acctcatgc tacagtttt ttatcgagt ttcaccgaa 
      aaataaatt agcgattgt taagtcgtt gcaaaggac 
      tgggcatat cgttgggat gactggtga tggagtgaa 
      tgatggtgt ggcgcttaa aaaagccga tattgggat 
      tgccatggg caaaaatgg gacggatgt gtgtaaaga 
      agccgcaga tatgatact cgttgatga tgattttta 
      cacaattat tgcagcaat tgaagaagg gaagagcat 
      attttacaa catccgaaa ctttgtcag attccaatt 
      atctacctc tattgcggc tttgtctct aatcgcact 
      agcgacttt gatgggaat tccgaatcc gttaaacgc 
      catgcaaat tttgtggat caacattat aatggacgg 
      tcctccggc ccaaagttt gggcgtaga accggtcga 
      aaaagacgt ggtaaaaca aaaacctcg aaatactaa 
      agaaccaat gatcactaa aaaactaat cggaaacgt 
      tctactttc cgccttatt tataatagc tggaacgtt 
      atgggtgtt caaaaaaga gatgagttc ggaaggtat 
      tacagcacg cgatacaac aatgacgtt cacttgttt 
      cgtattttt cgacatgtg gaacgcttt atcctgtcg 
      gtcgcaaac caagagcgt tttccagat tggtttgtt 
      ctccaataa aatgtttct ggtggctgt caccctctc 
      cgtgattgg acaacttct cgttgttta ctttccacc 
      tttgcagcg aattttcca aaccgaagc tctcacatt 
      atgggacat ggtattttt aacaacact aacgtcttc 
      ggtttgggt cttttcgga gataaagaa gctgatcga 
      gcggtttct cgaacagcg agggaaagg caagcgatc 
      gcctttgga gtatgtatg acataaggg ggggcctgg 
      ggcgaagtc ccgctgcta ttagctgcc tcttcaact 
      ttcccccct ttagatagg cccccttag aatgtgcaa 
      aaatctgat acacatcgt ttatttacc taaggaaaa 
      aatcttaca atttacgac taaagacgg ttttcaact 
      gtgttgatt tatagaaac caagaccta aaataaatt 
      tattaagga gtttcagaa attgagata aaagcaatc 
      tcgtaatcg cgtagaata gtaagtgta tgtgttagc 
      tttcgtaac ataatcaag atgtgtgtg tttgcaatt 
      ttatttaaa tgatttgca cattttaat ggaccaagt 
      tatgatttt gcagctaat aggtgttat tccaatttt 
      gtttctcag gctgaggtt taagtgtta ttttaaggc 
      gaatttatg agggtgtat acttaatat ctaaattta 
      cacaaaata gtgaaggga acaattcag ctttattgt 
      tacaattta ccgtagctt tcctattgg tgtaggaga 
      aagttgtta cactaatta ggaggctgc gcattttac 
      ccagtccct tttctgatt ttatttata aaacatttt 
      acatttgtt gggtgaatt ttacaattc atgttcatg 
      acaggaggt tgtgctatt tatagaatt ttgtttcaa 
      tcaaaaata tatcgacgt cgattcatc tttacatgt 
      gtgtgtatt tatatctac acttttgta ctcccaaat 
      tgagccatt cctgtattt gtggcattt cacaatatt 
      tgagaatca aatttgtta ttgagcttt ccaattatg 
      ttgtgctgc taggtgtat aagtagata atgtgtgta 
      atgttcaat atagcacgt gtaagtact ttcacatct 
      taagtgtta aaacatctg caagtactt tttaatagt 
      tctgtgata cactaaata taattaaat ttaagtaac 
      gtttcaacg tattcttgc ttgttctgt tacgtttca 
      aatgtgtaa tgagtattt agaacgttg tacattttt 
      gtaacattt caaatataa tttatattt attatgatc 
      gcgaatttc acaatttta tagaactgt tccactttt 
      caaaatcat acacaattc agatattat ttcatgttt 
      gatgtctcc actatttat tgttcatat ggaagcaaa 
      tttgcgttg tatactact ttctgacat taaattcat 
      ttttttaaa taaaagttc ctctatctt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY