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Gene information
General information
TC011524 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 3730632..3733050
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011524
    iBeetle screen iB_05047

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011524 RA
      MADANELPE NSRGKSHTA PFSEESDSE ESVQPVLLD 
      LGDDLSRVS DSNRLGFQS AGASGSTEP PRREDNPFS 
      FKHFLRSDV NSYQNKGAR PKVYCEGRP VSSVSDLEF 
      APSQETKQT RIVPEYSSA LPDFVQDHL VIEQCYLNN 
      STNSNFNLD INLPDFTPS RDSININRV NIDSSHNVN 
      RGCDISNNL SIPLDLPVR PQAGFPLDL PISNSPQNG 
      NRNCVNSEV GVSKSLPDF LADGAVCPQ NPDGPQVES 
      PERECERLR RELEVCRGQ LVERTRQVE NLTRELEIA 
      RNKEHDYTQ NLAKALEQV EKNLEKSNM RTASAENTI 
      VKLRQEIKS LVSQLNSLK SENLLLRGE EGAAGGHDY 
      HTPNEMHSQ RLAQELKAA ASTAEHSLR QLLTGVDNL 
      RIMAASLEN MHRIEEKRE PFLDLDEDT GPAL
    CDS sequence
    • >TC011524 RA
      atggctgat gcgaatgaa ttgccggaa aacagtcgt 
      ggaaagtct cacacagcc ccatttagt gaggagtct 
      gattccgag gagtccgtt caacctgtc cttctggac 
      ttgggcgac gatttgagc cgagtgtct gactcaaat 
      aggttagga tttcaaagt gctggagct tcaggtagt 
      accgagccc cccaggcga gaagacaac ccgttttcg 
      ttcaaacat tttttacgt agtgatgta aatagctat 
      caaaataag ggggcacgg cctaaggtt tactgcgag 
      gggcgtccg gtgtcgtcc gtctccgac ttggaattc 
      gccccctcg caggagaca aaacagaca aggatcgtt 
      cctgagtac tcatctgcc ttacctgat ttcgttcaa 
      gaccactta gttatagaa cagtgttat ttaaacaat 
      tctacgaat agcaatttt aatttggat ataaacctg 
      ccggatttt accccaagt agggacagc attaacata 
      aatagagta aatatcgat tctagtcat aacgttaat 
      cgaggatgt gatattagt aacaatttg tcgataccg 
      ttggatttg cctgtgagg ccgcaggct ggcttccct 
      ctagacctc ccaatatct aattcgccc caaaatggt 
      aaccggaat tgtgttaac agtgaagtt ggagtatct 
      aaaagtttg ccagatttc ttagccgat ggtgcggtg 
      tgtcctcag aaccctgat ggacctcaa gttgaaagt 
      ccagagagg gagtgtgag aggttgagg cgggaactg 
      gaagtttgt agaggacag ttggtcgag aggacacgt 
      caagttgag aatctcaca agggaacta gaaattgca 
      cgaaacaag gaacatgat tacacacaa aatctcgcc 
      aaagcgctt gaacaagtc gagaaaaat ttggaaaag 
      agcaacatg agaacggca agtgctgaa aataccatt 
      gtaaaattg cggcaagaa ataaaatcg cttgtgagt 
      caattgaac agtttaaag tcggaaaat ttgctactt 
      cgtggggaa gagggtgca gctgggggg cacgattat 
      catactccg aatgaaatg cattcgcag agattagct 
      caagaattg aaagctgca gcgagtact gcagaacac 
      tcattaagg caattgtta actggtgtt gataattta 
      agaataatg gcggcaagt ttagaaaac atgcatcgc 
      attgaagag aaaagagag cctttctta gatttagat 
      gaggataca ggtccagca ttgtaa
    mRNA sequence
    • >TC011524 RA
      gtaatgatc tgttagggc gtatgacac aacaacaga 
      accctgact aacgactag aggttgaaa taaacttgt 
      atttttgaa aattttatc aatttctaa ttcgaatta 
      gtaggcgcg ctcgcagtt gacaaaact gacacttga 
      cagcatcac aaaagttga cgtttattt attttgagc 
      gtgtatgat aatggctga tgcgaatga attgccgga 
      aaacagtcg tggaaagtc tcacacagc cccatttag 
      tgaggagtc tgattccga ggagtccgt tcaacctgt 
      ccttctgga cttgggcga cgatttgag ccgagtgtc 
      tgactcaaa taggttagg atttcaaag tgctggagc 
      ttcaggtag taccgagcc ccccaggcg agaagacaa 
      cccgttttc gttcaaaca ttttttacg tagtgatgt 
      aaatagcta tcaaaataa gggggcacg gcctaaggt 
      ttactgcga ggggcgtcc ggtgtcgtc cgtctccga 
      cttggaatt cgccccctc gcaggagac aaaacagac 
      aaggatcgt tcctgagta ctcatctgc cttacctga 
      tttcgttca agaccactt agttataga acagtgtta 
      tttaaacaa ttctacgaa tagcaattt taatttgga 
      tataaacct gccggattt taccccaag tagggacag 
      cattaacat aaatagagt aaatatcga ttctagtca 
      taacgttaa tcgaggatg tgatattag taacaattt 
      gtcgatacc gttggattt gcctgtgag gccgcaggc 
      tggcttccc tctagacct cccaatatc taattcgcc 
      ccaaaatgg taaccggaa ttgtgttaa cagtgaagt 
      tggagtatc taaaagttt gccagattt cttagccga 
      tggtgcggt gtgtcctca gaaccctga tggacctca 
      agttgaaag tccagagag ggagtgtga gaggttgag 
      gcgggaact ggaagtttg tagaggaca gttggtcga 
      gaggacacg tcaagttga gaatctcac aagggaact 
      agaaattgc acgaaacaa ggaacatga ttacacaca 
      aaatctcgc caaagcgct tgaacaagt cgagaaaaa 
      tttggaaaa gagcaacat gagaacggc aagtgctga 
      aaataccat tgtaaaatt gcggcaaga aataaaatc 
      gcttgtgag tcaattgaa cagtttaaa gtcggaaaa 
      tttgctact tcgtgggga agagggtgc agctggggg 
      gcacgatta tcatactcc gaatgaaat gcattcgca 
      gagattagc tcaagaatt gaaagctgc agcgagtac 
      tgcagaaca ctcattaag gcaattgtt aactggtgt 
      tgataattt aagaataat ggcggcaag tttagaaaa 
      catgcatcg cattgaaga gaaaagaga gcctttctt 
      agatttaga tgaggatac aggtccagc attgtaatg 
      gtaaaaata tataagaca actgatttt tgatattgt 
      acatagatc tacatatat gtagcttaa tttattaca 
      agtagggat gtacgctag ttttttcat tcatgtgat 
      tttttattt agtgatagc tattttgat attaacacc 
      aactgacta taagccatc tatcaacag atctttgta 
      agtgtgatg ctacttata catcacatt atttaatat 
      tacggctgt atacacttg tgttatggt ttgtaatat 
      actgtatat catctc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY