Status
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Gene information
General information
TC011521 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 3750378..3753440
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011521
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05046

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011521 RA
      MSHKTYDSE WKKVQQLLA ETLQSEAVQ VDRREQRQL 
      LATLYVRYI LVSNVLSEC VDQMVQPQK RRLIRKLLE 
      ATLGRILEL KYDLVEADL SEWTHCGDV LEQLKLTPE 
      QSELKVPSC FRNERRKEL EYQKNLIES VLGKLGFLD 
      KLEIKPPMS EQQAILIIQ VHERARQGR LRAQFMREI 
      RNMKEKTKP VVGEGGDED EQRGGISLP AALRIQKIW 
      RGYVARRAT RRRKLQEML LIGMIPPPK TQSAEVLKD 
      LEIKAYRRS LQEKRQLEY ENDVKKCRE HMEKYQRGV 
      VLEQLSDQV RGWLHEYKA QTGKIPEYT GSERAASRL 
      LFSRQGTDS EMSKSTQIS SKDSKTKKD KAPKQKEAK 
      QEGEEGEEV STKAMVSVF VPELNARKE EYDEIWKNK 
      DESGNPKQF HYMDIIEHE QMTSMENEL RKVVDEMMK 
      GELLLLQEA MDKDRGHKG KKKSSKKTR KGGKKGKKK 
      KEKDLTPDR TTESLFEEL VANGIIKKC PEVYLDDFK 
      GERSYSYPV VYNKGSERP IALGDMRQI VAEYCVIPL 
      LSPQLHQTT PHIKSLMIG GPKGSGKDM LVHAICNEA 
      GAVLFDLTP SNIVGKYPG KSGLTMLIH LVVKVSRLL 
      QPSVIYMDC AERPFVKKI PKTDKTDPK RLKKDLPKI 
      VKNFSPEDR VMLIGVSNC PWESDQKLL QQVYQKFIL 
      VPKPDYSSR YCVWSYLLS KYSAISWQF DTSVVSKIS 
      DGFTIGSIV STFEEVMTI KRMLQLRVH PLSPLELVN 
      VLCKKIPIY KEEEEAIET WWAKTPLCR RRARAVEIL 
      LEEEAELQA KQASTKN
    CDS sequence
    • >TC011521 RA
      atgtctcac aaaacctac gattccgaa tggaaaaaa 
      gtccaacaa ttactcgca gaaacgctt cagagcgaa 
      gctgtgcaa gtggacaga cgagaacag cgccaatta 
      ctggccact ctctacgtg agatacatt ttagtatca 
      aacgtcctt tcggaatgc gttgatcaa atggtacag 
      ccccaaaaa cgcagactg atccgaaaa ctcctggaa 
      gcaacactt ggaagaatc ctcgagctg aaatacgac 
      ctcgtggaa gccgatcta agcgagtgg acgcactgt 
      ggcgacgtt ctcgaacaa ctaaaacta acacctgaa 
      caaagtgaa ttgaaagtc ccttcttgt ttccgaaac 
      gagcgccga aaagaacta gagtatcaa aaaaactta 
      atagagagc gttttgggc aagttgggt ttccttgat 
      aagttagaa atcaaaccc ccgatgagt gaacaacaa 
      gccatttta ataatacaa gtgcacgag agggcgaga 
      cagggccgg ctcagggca cagttcatg cgagaaatc 
      aggaacatg aaagagaag actaagcca gtggtgggt 
      gaagggggt gacgaagac gaacaaagg ggagggatt 
      agcttaccg gcagctttg cgcattcag aaaatatgg 
      agagggtat gtggcccgc agggccact cgacgaaga 
      aaactccaa gaaatgttg ctgattggg atgatacca 
      ccaccaaaa actcagagt gctgaagta ctcaaggac 
      ttagaaatt aaagcgtac cgacgatct ttacaggaa 
      aaaaggcag ttggaatat gaaaatgac gtgaagaaa 
      tgtagggaa cacatggag aagtaccaa agaggggtg 
      gttttggag cagttgagt gaccaagtg aggggttgg 
      ttgcacgaa tataaggca caaactggg aaaatacca 
      gagtacacg ggatctgag agagctgct tcgagatta 
      ttgtttagc agacaaggt acggatagt gaaatgagc 
      aaatccacc caaatctcg tccaaagac tcgaaaact 
      aaaaaagac aaggcgcca aaacagaaa gaagccaag 
      caagaaggc gaagaagga gaggaagtc agtactaaa 
      gcaatggtt tcggtgttt gtgcccgaa ctgaatgct 
      cgcaaagag gaatacgat gagatttgg aagaataaa 
      gacgagtcg ggaaatccc aaacagttc cactacatg 
      gatataata gaacacgaa caaatgacc agtatggaa 
      aatgagcta aggaaagtg gtggatgaa atgatgaaa 
      ggggagtta ttattgttg caggaagca atggataaa 
      gatagaggg cataagggg aaaaagaaa agtagtaaa 
      aaaactaga aaaggcggt aagaaagga aagaagaaa 
      aaggagaag gacttgact cctgaccga actactgag 
      tcacttttt gaggaattg gtagcaaac gggataatc 
      aagaagtgt ccagaggtg tatttagac gacttcaaa 
      ggagagcgt tcttacagc tatccagtg gtgtacaac 
      aaagggagt gaacgcccg attgctctg ggagacatg 
      agacaaatt gtggctgaa tattgcgtg ataccactg 
      ctttcgccc caattacac caaactact ccccacata 
      aagtcgtta atgataggt ggccccaaa ggaagcggc 
      aaagatatg ctagtccat gcgatttgt aacgaagca 
      ggagcggtt ctttttgac ttaactccg tccaacatt 
      gtggggaaa tatcccgga aagtcagga ttgacgatg 
      ttaattcac ttggtggtc aaagtgtca agactgttg 
      caaccatcg gtgatttac atggactgt gccgaacga 
      cctttcgtg aaaaaaatc ccaaaaacg gacaaaacc 
      gaccccaaa cgactcaaa aaagattta ccaaaaatc 
      gtaaaaaat ttcagtccc gaagatcgc gtaatgttg 
      ataggagtt tcaaactgt ccgtgggag agcgaccag 
      aaattgctc caacaagtg taccagaaa tttattctt 
      gttccgaag cccgattat agctcacgg tattgcgtt 
      tggagttat cttttgagc aaatatagc gccataagc 
      tggcagttt gacaccagt gttgtcagt aaaatctcc 
      gatggattt acgatcggc tcaattgtg agtactttt 
      gaggaggtc atgacaata aaaagaatg ctgcaactg 
      agagtacat ccgctgagt cctttggaa ctggtcaat 
      gtgttgtgt aaaaaaata ccgatttat aaggaagaa 
      gaagaagca attgagact tggtgggcg aaaacacca 
      ctctgccga agaagagca cgagctgtt gagattttg 
      ctggaggaa gaagccgag cttcaagct aaacaagcg 
      agcactaaa aactag
    mRNA sequence
    • >TC011521 RA
      aatactcca aattattga tcaaacaat ttcgtgata 
      agtattata gttgtttcg aaaaatgtc tcacaaaac 
      ctacgattc cgaatggaa aaaagtcca acaattact 
      cgcagaaac gcttcagag cgaagctgt gcaagtgga 
      cagacgaga acagcgcca attactggc cactctcta 
      cgtgagata cattttagt atcaaacgt cctttcgga 
      atgcgttga tcaaatggt acagcccca aaaacgcag 
      actgatccg aaaactcct ggaagcaac acttggaag 
      aatcctcga gctgaaata cgacctcgt ggaagccga 
      tctaagcga gtggacgca ctgtggcga cgttctcga 
      acaactaaa actaacacc tgaacaaag tgaattgaa 
      agtcccttc ttgtttccg aaacgagcg ccgaaaaga 
      actagagta tcaaaaaaa cttaataga gagcgtttt 
      gggcaagtt gggtttcct tgataagtt agaaatcaa 
      acccccgat gagtgaaca acaagccat tttaataat 
      acaagtgca cgagagggc gagacaggg ccggctcag 
      ggcacagtt catgcgaga aatcaggaa catgaaaga 
      gaagactaa gccagtggt gggtgaagg gggtgacga 
      agacgaaca aaggggagg gattagctt accggcagc 
      tttgcgcat tcagaaaat atggagagg gtatgtggc 
      ccgcagggc cactcgacg aagaaaact ccaagaaat 
      gttgctgat tgggatgat accaccacc aaaaactca 
      gagtgctga agtactcaa ggacttaga aattaaagc 
      gtaccgacg atctttaca ggaaaaaag gcagttgga 
      atatgaaaa tgacgtgaa gaaatgtag ggaacacat 
      ggagaagta ccaaagagg ggtggtttt ggagcagtt 
      gagtgacca agtgagggg ttggttgca cgaatataa 
      ggcacaaac tgggaaaat accagagta cacgggatc 
      tgagagagc tgcttcgag attattgtt tagcagaca 
      aggtacgga tagtgaaat gagcaaatc cacccaaat 
      ctcgtccaa agactcgaa aactaaaaa agacaaggc 
      gccaaaaca gaaagaagc caagcaaga aggcgaaga 
      aggagagga agtcagtac taaagcaat ggtttcggt 
      gtttgtgcc cgaactgaa tgctcgcaa agaggaata 
      cgatgagat ttggaagaa taaagacga gtcgggaaa 
      tcccaaaca gttccacta catggatat aatagaaca 
      cgaacaaat gaccagtat ggaaaatga gctaaggaa 
      agtggtgga tgaaatgat gaaagggga gttattatt 
      gttgcagga agcaatgga taaagatag agggcataa 
      ggggaaaaa gaaaagtag taaaaaaac tagaaaagg 
      cggtaagaa aggaaagaa gaaaaagga gaaggactt 
      gactcctga ccgaactac tgagtcact ttttgagga 
      attggtagc aaacgggat aatcaagaa gtgtccaga 
      ggtgtattt agacgactt caaaggaga gcgttctta 
      cagctatcc agtggtgta caacaaagg gagtgaacg 
      cccgattgc tctgggaga catgagaca aattgtggc 
      tgaatattg cgtgatacc actgctttc gccccaatt 
      acaccaaac tactcccca cataaagtc gttaatgat 
      aggtggccc caaaggaag cggcaaaga tatgctagt 
      ccatgcgat ttgtaacga agcaggagc ggttctttt 
      tgacttaac tccgtccaa cattgtggg gaaatatcc 
      cggaaagtc aggattgac gatgttaat tcacttggt 
      ggtcaaagt gtcaagact gttgcaacc atcggtgat 
      ttacatgga ctgtgccga acgaccttt cgtgaaaaa 
      aatcccaaa aacggacaa aaccgaccc caaacgact 
      caaaaaaga tttaccaaa aatcgtaaa aaatttcag 
      tcccgaaga tcgcgtaat gttgatagg agtttcaaa 
      ctgtccgtg ggagagcga ccagaaatt gctccaaca 
      agtgtacca gaaatttat tcttgttcc gaagcccga 
      ttatagctc acggtattg cgtttggag ttatctttt 
      gagcaaata tagcgccat aagctggca gtttgacac 
      cagtgttgt cagtaaaat ctccgatgg atttacgat 
      cggctcaat tgtgagtac ttttgagga ggtcatgac 
      aataaaaag aatgctgca actgagagt acatccgct 
      gagtccttt ggaactggt caatgtgtt gtgtaaaaa 
      aataccgat ttataagga agaagaaga agcaattga 
      gacttggtg ggcgaaaac accactctg ccgaagaag 
      agcacgagc tgttgagat tttgctgga ggaagaagc 
      cgagcttca agctaaaca agcgagcac taaaaacta 
      gtcgttcat tcaattaat ttgtcataa gttttaact 
      taaaaaaat agaataatt acaacaaat tatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY