Status
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Gene information
General information
TC011514 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 3799714..3804808
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011514
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05045

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    head appendages partially not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    head not present - Penetrance (percentage of animals/eggs on slide): more than 80%

    legs shortened - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    thorax not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011514 RA
      MASEQPVKK KLRRGDSPD APDEPDFIH NGVSALHLT 
      YPPTVNEED LNTVNAQFH GGKVFRTSD VSDSDKDPP 
      TVENITIQP APDIKQQML VDEEESETF YSYLVKQCL 
      CDIPEGVLR KPFEGHQVV GEAGTRLAM LSEWGTPQV 
      LQFLSNVHL LFEVYLKQN NNGHICSKI VTICDTFVR 
      NEHNLIEQI LSLCETRDK YINYLAAKV FSSFLIIAK 
      TNINNEWLE TIVGFLTIE NTDYVKMNF ALEIIKRVV 
      EWKNVDIHV LEEPQSQDV AGSSSQNVE INVNCVSVP 
      YSDAESYDT SGIKGLIIK SLEAKWPEL ISKIQYLIT 
      QNNSIEAQT CVLTFLSLW ESTISVKAN LSVIDTKPF 
      YAHLENFVG LLNSNLPPI IWKQLLSLF NEVLCYGST 
      LALQDMLPE DTCQLAHLI VRYVKDFRL LDSLPYRRS 
      EGYTVNSFV GTIPSTQPT QSNIDKTLL QKMTLLVLK 
      SVAITIKET RSDSSDSSV GSEDYDFSH DMQLIARSI 
      RDVLKKVDV FIKLSLEFH PETPFSKIL IHLFSDQDD 
      YMIEAMVCT LDIKTGISY QNALAPDFI AMLNPVHSF 
      IEFLKIVSH DSDVLLDYL VSNETCFLL YLLRFLKYT 
      RRNWDVFVN SCYEGGASR TNELDNTMT VLIRLKMQI 
      DRLVSKDLF PYNINPILR LLEICENLY EGNEYS
    CDS sequence
    • >TC011514 RA
      atggcctct gaacagccg gtgaagaag aagctgcga 
      cgcggtgac agccccgac gcccccgat gagcccgat 
      ttcatccac aacggggtc tccgccctg cacctgacc 
      tacccccct actgtgaac gaggaggat ctcaacacc 
      gtcaacgcg cagttccat gggggcaaa gtgttccgg 
      actagtgac gtgagtgac agtgacaag gaccccccg 
      actgtggaa aacatcacc atacagccg gcccccgac 
      atcaagcag cagatgctg gtggacgag gaggaaagt 
      gagactttt tacagctat ttggtgaag cagtgcctc 
      tgcgacatc cccgagggc gtcctgcgc aagcccttc 
      gaggggcac caggtcgtg ggggaggcc gggacccgg 
      ctggcgatg ctaagcgag tggggcacc ccccaagtg 
      ctccagttc ctctcaaac gtgcatttg ttgttcgaa 
      gtttacttg aaacagaac aacaacgga cacatctgc 
      tccaaaatc gtgacgatt tgtgacacc ttcgtacgc 
      aacgaacac aacttaatc gagcagatt ttatcgctt 
      tgtgagaca cgggacaag tatatcaat tatctggcc 
      gccaaagtg ttcagtagt tttcttatt attgccaaa 
      actaacatc aacaatgag tggctcgaa acaattgtc 
      ggtttttta acaattgaa aacaccgac tatgtcaaa 
      atgaacttc gcgcttgaa attattaag cgagtcgtg 
      gagtggaag aacgtcgat atccacgtc ctggaggag 
      ccccaaagt caggacgtg gccgggagt agctcccaa 
      aacgtggaa atcaacgta aattgcgtg tcggttccg 
      tacagcgat gccgaaagc tacgacact tcggggatc 
      aagggcttg attattaaa agtctggaa gcgaaatgg 
      ccagaattg atcagtaaa attcaatac ttgatcact 
      cagaacaat tcaatcgaa gcacaaact tgcgttttg 
      acgtttctg tctctttgg gaaagcacc atttcggtc 
      aaagccaac ttaagtgtg attgacacg aaaccgttc 
      tacgcccat ttggagaat tttgttggg ttgctcaac 
      agtaaccta ccaccaatc atttggaag cagttactg 
      tccctcttt aacgaggtt ttgtgctat gggagcact 
      ctggcattg caggacatg ctcccggag gacacgtgc 
      cagctggcc cacttgatc gtgcgctac gtcaaagac 
      ttccgcctg ctcgacagc ctgccgtac aggcgctca 
      gagggctac actgtgaac agcttcgtg gggacgatc 
      cccagcacg cagccgacg cagtccaac atcgacaag 
      actctcttg cagaagatg acgctcctg gtgctgaag 
      agcgtggcc atcaccatc aaggagacg cgctcggac 
      agctcggac tccagcgtg gggtcggag gactacgac 
      ttctcgcac gacatgcag ctgatcgcg cgctcgatc 
      cgcgacgtg ctcaagaag gtggacgtc ttcatcaag 
      ctcagcttg gagtttcac cccgaaacg cccttctcg 
      aaaatactc atccatttg tttagcgat caagacgat 
      tacatgatc gaggccatg gtttgtacg ctggatatt 
      aagacgggg atctcgtac cagaacgcg ttggcgccc 
      gattttatc gcgatgctg aatccggtc cactcgttt 
      atcgagttt ttgaaaatt gttagtcat gattcggac 
      gtactatta gattattta gtcagtaat gagacgtgt 
      ttcttgttg tatctgttg cggttcttg aagtatacg 
      aggaggaat tgggatgtt tttgtaaat agttgttac 
      gagggcgga gcgtcgagg accaatgag cttgataat 
      acaatgacg gttttgata agacttaaa atgcagatt 
      gatcgactc gtgtcgaag gatcttttt ccttataat 
      attaacccc attttaaga ctgcttgag atttgcgag 
      aatctttac gaaggcaac gagtacagc tga
    mRNA sequence
    • >TC011514 RA
      cgcaatgga gttgtgtgt gtgtataaa tttggtgtt 
      ttttaaaaa catttattt attttagcc tagttttca 
      aagaaaatt tccacaaac caagttttc tggcattag 
      taccaccgc acgcaacta atgaagcct ccagaaggg 
      tcctccgga gtgcacgaa cgccactct cgaattaac 
      tactggata aaattgtta cacaaagtc cccggcgcc 
      cagccgttg taactcaac acccggccc ttcgtcccc 
      gctctaatg gttctaaga caccagcag gagagtaaa 
      atggcctct gaacagccg gtgaagaag aagctgcga 
      cgcggtgac agccccgac gcccccgat gagcccgat 
      ttcatccac aacggggtc tccgccctg cacctgacc 
      tacccccct actgtgaac gaggaggat ctcaacacc 
      gtcaacgcg cagttccat gggggcaaa gtgttccgg 
      actagtgac gtgagtgac agtgacaag gaccccccg 
      actgtggaa aacatcacc atacagccg gcccccgac 
      atcaagcag cagatgctg gtggacgag gaggaaagt 
      gagactttt tacagctat ttggtgaag cagtgcctc 
      tgcgacatc cccgagggc gtcctgcgc aagcccttc 
      gaggggcac caggtcgtg ggggaggcc gggacccgg 
      ctggcgatg ctaagcgag tggggcacc ccccaagtg 
      ctccagttc ctctcaaac gtgcatttg ttgttcgaa 
      gtttacttg aaacagaac aacaacgga cacatctgc 
      tccaaaatc gtgacgatt tgtgacacc ttcgtacgc 
      aacgaacac aacttaatc gagcagatt ttatcgctt 
      tgtgagaca cgggacaag tatatcaat tatctggcc 
      gccaaagtg ttcagtagt tttcttatt attgccaaa 
      actaacatc aacaatgag tggctcgaa acaattgtc 
      ggtttttta acaattgaa aacaccgac tatgtcaaa 
      atgaacttc gcgcttgaa attattaag cgagtcgtg 
      gagtggaag aacgtcgat atccacgtc ctggaggag 
      ccccaaagt caggacgtg gccgggagt agctcccaa 
      aacgtggaa atcaacgta aattgcgtg tcggttccg 
      tacagcgat gccgaaagc tacgacact tcggggatc 
      aagggcttg attattaaa agtctggaa gcgaaatgg 
      ccagaattg atcagtaaa attcaatac ttgatcact 
      cagaacaat tcaatcgaa gcacaaact tgcgttttg 
      acgtttctg tctctttgg gaaagcacc atttcggtc 
      aaagccaac ttaagtgtg attgacacg aaaccgttc 
      tacgcccat ttggagaat tttgttggg ttgctcaac 
      agtaaccta ccaccaatc atttggaag cagttactg 
      tccctcttt aacgaggtt ttgtgctat gggagcact 
      ctggcattg caggacatg ctcccggag gacacgtgc 
      cagctggcc cacttgatc gtgcgctac gtcaaagac 
      ttccgcctg ctcgacagc ctgccgtac aggcgctca 
      gagggctac actgtgaac agcttcgtg gggacgatc 
      cccagcacg cagccgacg cagtccaac atcgacaag 
      actctcttg cagaagatg acgctcctg gtgctgaag 
      agcgtggcc atcaccatc aaggagacg cgctcggac 
      agctcggac tccagcgtg gggtcggag gactacgac 
      ttctcgcac gacatgcag ctgatcgcg cgctcgatc 
      cgcgacgtg ctcaagaag gtggacgtc ttcatcaag 
      ctcagcttg gagtttcac cccgaaacg cccttctcg 
      aaaatactc atccatttg tttagcgat caagacgat 
      tacatgatc gaggccatg gtttgtacg ctggatatt 
      aagacgggg atctcgtac cagaacgcg ttggcgccc 
      gattttatc gcgatgctg aatccggtc cactcgttt 
      atcgagttt ttgaaaatt gttagtcat gattcggac 
      gtactatta gattattta gtcagtaat gagacgtgt 
      ttcttgttg tatctgttg cggttcttg aagtatacg 
      aggaggaat tgggatgtt tttgtaaat agttgttac 
      gagggcgga gcgtcgagg accaatgag cttgataat 
      acaatgacg gttttgata agacttaaa atgcagatt 
      gatcgactc gtgtcgaag gatcttttt ccttataat 
      attaacccc attttaaga ctgcttgag atttgcgag 
      aatctttac gaaggcaac gagtacagc tgatagctt 
      taattttta ttcggtttt tttatgtgt atatagttg 
      aaagtttcc gtgatatag tacaaaacc ctcccattt 
      cttctaatt tattttttt atacatttt tcaaacagg 
      acagacaac atgtccagt taccacgta ttttgcata 
      attggcgat tgtgttttt gatgaaaaa tttcattca 
      acgcatgat aaatattat taattaaaa tgaaacgat 
      cgcacagat aacgaaagc aaatagttt caaaataat 
      taaactaat gaaatgttt taattgggg aaatttcgc 
      aactgggcc aattataat tatgaaatt gttagtgtt 
      acccaaaaa acatgtgtc cctaatctt acagatgac 
      aaaaaatca caaaagcca ctgtttttt taataacta 
      ccatatttt ttctaatta actagcgta aaacaattt 
      aatgctagt tgcaaaaca gtcacttta taattggtt 
      ttctctttt aatttctgg gttttcttg agattcaat 
      ttgtcacta gggtccaat ctaacagag aatcacacg 
      aaaatacag caagtttaa ggtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY