Status
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Gene information
General information
TC011514 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 3799714..3804808
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC011514
    iBeetle screen iB_05045

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     head appendages partially not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    head not present - Penetrance (percentage of animals/eggs on slide): more than 80%

    legs shortened - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    thorax not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    larva lethality late (L7) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    larva lethality late (L7) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011514 RA
      MASEQPVKK KLRRGDSPD APDEPDFIH NGVSALHLT 
YPPTVNEED LNTVNAQFH GGKVFRTSD VSDSDKDPP 
TVENITIQP APDIKQQML VDEEESETF YSYLVKQCL 
CDIPEGVLR KPFEGHQVV GEAGTRLAM LSEWGTPQV 
LQFLSNVHL LFEVYLKQN NNGHICSKI VTICDTFVR 
NEHNLIEQI LSLCETRDK YINYLAAKV FSSFLIIAK 
TNINNEWLE TIVGFLTIE NTDYVKMNF ALEIIKRVV 
EWKNVDIHV LEEPQSQDV AGSSSQNVE INVNCVSVP 
YSDAESYDT SGIKGLIIK SLEAKWPEL ISKIQYLIT 
QNNSIEAQT CVLTFLSLW ESTISVKAN LSVIDTKPF 
YAHLENFVG LLNSNLPPI IWKQLLSLF NEVLCYGST 
LALQDMLPE DTCQLAHLI VRYVKDFRL LDSLPYRRS 
EGYTVNSFV GTIPSTQPT QSNIDKTLL QKMTLLVLK 
SVAITIKET RSDSSDSSV GSEDYDFSH DMQLIARSI 
RDVLKKVDV FIKLSLEFH PETPFSKIL IHLFSDQDD 
YMIEAMVCT LDIKTGISY QNALAPDFI AMLNPVHSF 
IEFLKIVSH DSDVLLDYL VSNETCFLL YLLRFLKYT 
RRNWDVFVN SCYEGGASR TNELDNTMT VLIRLKMQI 
DRLVSKDLF PYNINPILR LLEICENLY EGNEYS
    CDS sequence
    • >TC011514 RA
      atggcctct gaacagccg gtgaagaag aagctgcga 
cgcggtgac agccccgac gcccccgat gagcccgat 
ttcatccac aacggggtc tccgccctg cacctgacc 
tacccccct actgtgaac gaggaggat ctcaacacc 
gtcaacgcg cagttccat gggggcaaa gtgttccgg 
actagtgac gtgagtgac agtgacaag gaccccccg 
actgtggaa aacatcacc atacagccg gcccccgac 
atcaagcag cagatgctg gtggacgag gaggaaagt 
gagactttt tacagctat ttggtgaag cagtgcctc 
tgcgacatc cccgagggc gtcctgcgc aagcccttc 
gaggggcac caggtcgtg ggggaggcc gggacccgg 
ctggcgatg ctaagcgag tggggcacc ccccaagtg 
ctccagttc ctctcaaac gtgcatttg ttgttcgaa 
gtttacttg aaacagaac aacaacgga cacatctgc 
tccaaaatc gtgacgatt tgtgacacc ttcgtacgc 
aacgaacac aacttaatc gagcagatt ttatcgctt 
tgtgagaca cgggacaag tatatcaat tatctggcc 
gccaaagtg ttcagtagt tttcttatt attgccaaa 
actaacatc aacaatgag tggctcgaa acaattgtc 
ggtttttta acaattgaa aacaccgac tatgtcaaa 
atgaacttc gcgcttgaa attattaag cgagtcgtg 
gagtggaag aacgtcgat atccacgtc ctggaggag 
ccccaaagt caggacgtg gccgggagt agctcccaa 
aacgtggaa atcaacgta aattgcgtg tcggttccg 
tacagcgat gccgaaagc tacgacact tcggggatc 
aagggcttg attattaaa agtctggaa gcgaaatgg 
ccagaattg atcagtaaa attcaatac ttgatcact 
cagaacaat tcaatcgaa gcacaaact tgcgttttg 
acgtttctg tctctttgg gaaagcacc atttcggtc 
aaagccaac ttaagtgtg attgacacg aaaccgttc 
tacgcccat ttggagaat tttgttggg ttgctcaac 
agtaaccta ccaccaatc atttggaag cagttactg 
tccctcttt aacgaggtt ttgtgctat gggagcact 
ctggcattg caggacatg ctcccggag gacacgtgc 
cagctggcc cacttgatc gtgcgctac gtcaaagac 
ttccgcctg ctcgacagc ctgccgtac aggcgctca 
gagggctac actgtgaac agcttcgtg gggacgatc 
cccagcacg cagccgacg cagtccaac atcgacaag 
actctcttg cagaagatg acgctcctg gtgctgaag 
agcgtggcc atcaccatc aaggagacg cgctcggac 
agctcggac tccagcgtg gggtcggag gactacgac 
ttctcgcac gacatgcag ctgatcgcg cgctcgatc 
cgcgacgtg ctcaagaag gtggacgtc ttcatcaag 
ctcagcttg gagtttcac cccgaaacg cccttctcg 
aaaatactc atccatttg tttagcgat caagacgat 
tacatgatc gaggccatg gtttgtacg ctggatatt 
aagacgggg atctcgtac cagaacgcg ttggcgccc 
gattttatc gcgatgctg aatccggtc cactcgttt 
atcgagttt ttgaaaatt gttagtcat gattcggac 
gtactatta gattattta gtcagtaat gagacgtgt 
ttcttgttg tatctgttg cggttcttg aagtatacg 
aggaggaat tgggatgtt tttgtaaat agttgttac 
gagggcgga gcgtcgagg accaatgag cttgataat 
acaatgacg gttttgata agacttaaa atgcagatt 
gatcgactc gtgtcgaag gatcttttt ccttataat 
attaacccc attttaaga ctgcttgag atttgcgag 
aatctttac gaaggcaac gagtacagc tga
    mRNA sequence
    • >TC011514 RA
      cgcaatgga gttgtgtgt gtgtataaa tttggtgtt 
ttttaaaaa catttattt attttagcc tagttttca 
aagaaaatt tccacaaac caagttttc tggcattag 
taccaccgc acgcaacta atgaagcct ccagaaggg 
tcctccgga gtgcacgaa cgccactct cgaattaac 
tactggata aaattgtta cacaaagtc cccggcgcc 
cagccgttg taactcaac acccggccc ttcgtcccc 
gctctaatg gttctaaga caccagcag gagagtaaa 
atggcctct gaacagccg gtgaagaag aagctgcga 
cgcggtgac agccccgac gcccccgat gagcccgat 
ttcatccac aacggggtc tccgccctg cacctgacc 
tacccccct actgtgaac gaggaggat ctcaacacc 
gtcaacgcg cagttccat gggggcaaa gtgttccgg 
actagtgac gtgagtgac agtgacaag gaccccccg 
actgtggaa aacatcacc atacagccg gcccccgac 
atcaagcag cagatgctg gtggacgag gaggaaagt 
gagactttt tacagctat ttggtgaag cagtgcctc 
tgcgacatc cccgagggc gtcctgcgc aagcccttc 
gaggggcac caggtcgtg ggggaggcc gggacccgg 
ctggcgatg ctaagcgag tggggcacc ccccaagtg 
ctccagttc ctctcaaac gtgcatttg ttgttcgaa 
gtttacttg aaacagaac aacaacgga cacatctgc 
tccaaaatc gtgacgatt tgtgacacc ttcgtacgc 
aacgaacac aacttaatc gagcagatt ttatcgctt 
tgtgagaca cgggacaag tatatcaat tatctggcc 
gccaaagtg ttcagtagt tttcttatt attgccaaa 
actaacatc aacaatgag tggctcgaa acaattgtc 
ggtttttta acaattgaa aacaccgac tatgtcaaa 
atgaacttc gcgcttgaa attattaag cgagtcgtg 
gagtggaag aacgtcgat atccacgtc ctggaggag 
ccccaaagt caggacgtg gccgggagt agctcccaa 
aacgtggaa atcaacgta aattgcgtg tcggttccg 
tacagcgat gccgaaagc tacgacact tcggggatc 
aagggcttg attattaaa agtctggaa gcgaaatgg 
ccagaattg atcagtaaa attcaatac ttgatcact 
cagaacaat tcaatcgaa gcacaaact tgcgttttg 
acgtttctg tctctttgg gaaagcacc atttcggtc 
aaagccaac ttaagtgtg attgacacg aaaccgttc 
tacgcccat ttggagaat tttgttggg ttgctcaac 
agtaaccta ccaccaatc atttggaag cagttactg 
tccctcttt aacgaggtt ttgtgctat gggagcact 
ctggcattg caggacatg ctcccggag gacacgtgc 
cagctggcc cacttgatc gtgcgctac gtcaaagac 
ttccgcctg ctcgacagc ctgccgtac aggcgctca 
gagggctac actgtgaac agcttcgtg gggacgatc 
cccagcacg cagccgacg cagtccaac atcgacaag 
actctcttg cagaagatg acgctcctg gtgctgaag 
agcgtggcc atcaccatc aaggagacg cgctcggac 
agctcggac tccagcgtg gggtcggag gactacgac 
ttctcgcac gacatgcag ctgatcgcg cgctcgatc 
cgcgacgtg ctcaagaag gtggacgtc ttcatcaag 
ctcagcttg gagtttcac cccgaaacg cccttctcg 
aaaatactc atccatttg tttagcgat caagacgat 
tacatgatc gaggccatg gtttgtacg ctggatatt 
aagacgggg atctcgtac cagaacgcg ttggcgccc 
gattttatc gcgatgctg aatccggtc cactcgttt 
atcgagttt ttgaaaatt gttagtcat gattcggac 
gtactatta gattattta gtcagtaat gagacgtgt 
ttcttgttg tatctgttg cggttcttg aagtatacg 
aggaggaat tgggatgtt tttgtaaat agttgttac 
gagggcgga gcgtcgagg accaatgag cttgataat 
acaatgacg gttttgata agacttaaa atgcagatt 
gatcgactc gtgtcgaag gatcttttt ccttataat 
attaacccc attttaaga ctgcttgag atttgcgag 
aatctttac gaaggcaac gagtacagc tgatagctt 
taattttta ttcggtttt tttatgtgt atatagttg 
aaagtttcc gtgatatag tacaaaacc ctcccattt 
cttctaatt tattttttt atacatttt tcaaacagg 
acagacaac atgtccagt taccacgta ttttgcata 
attggcgat tgtgttttt gatgaaaaa tttcattca 
acgcatgat aaatattat taattaaaa tgaaacgat 
cgcacagat aacgaaagc aaatagttt caaaataat 
taaactaat gaaatgttt taattgggg aaatttcgc 
aactgggcc aattataat tatgaaatt gttagtgtt 
acccaaaaa acatgtgtc cctaatctt acagatgac 
aaaaaatca caaaagcca ctgtttttt taataacta 
ccatatttt ttctaatta actagcgta aaacaattt 
aatgctagt tgcaaaaca gtcacttta taattggtt 
ttctctttt aatttctgg gttttcttg agattcaat 
ttgtcacta gggtccaat ctaacagag aatcacacg 
aaaatacag caagtttaa ggtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY