| General information | |||
| TC011477 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007425.3: 6838272..6863247 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC011477 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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|---|---|---|
| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02814 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
| Comment: 50% are affected. |
| Comment: 50% |
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05043 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 1 (16 dpi)
adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
- >TC011477 RA
MDSRIGLDF IVENPEYIR KLAAALDTS NVTVKKQVF ELLSALCVH NEEGHARAL DTLEHYKKL KGERYRLNV IVQELSRAT AVDYQTALV AFINCLIIS TPRLTDRIR RRNEFIGCH LLPVLNNLR KCAEAEPEL AVQLDVFDE QRDSDDSQS LQGPHGVDL NSPLDVFYA ILKQVAETP QEIPFLSIL QHLLRIDAK DAVSDIIWD TAERLVHRA TLLENREDA ARLLRAPSA QSKFFCHCH LRSESSPTR RPSLNTPQS PTPALPPPP PPPPNVPAV PPPVPAPPL KPAAPLPPP ECDSVIEKL PQQEIPVPK IKMKTFNWN KIPNNKVIG KNNIWTQVA YTHQHSPMA DLDWLEMEG LFCQQAPPA NAAASFRRE NTNSETLDR RIRKENCEI TLLDGKRSL NVNIFLKQF RSSNEDIIH LIREGEHDD IGAEKLKGL LKILPEVDE LDMLKSFDG DFTKLGNAE KFLIQLTNL SNYKLRIES MLLKEEFAS NMSYLEPSI KSMIMAAQD LMTNKPLQE VLYMILVAG NFLNAGGYA GNAAGVKLS SLQKITDIR ANKPNMNLI HFVALQAEK KNPKLLTFT DNISVLEDA AKTTVEQLQ NEINVLDVR IKKIKKQIE LATTEPEIK EQMTEFLQM AEREVSNLQ QNMEELEKV RVQLAEFFC EDTNSFKLE ECFRIFHGF YCKFKQAVG DNERRRIQE EQANARRRQ REELLASKR RQMENLGGT PDSDVPFID LQIYDSRHG FPMKKGWKT TNGTSEDEL SSVTGSPSL SRRRLGSFN GDPEKSPDI TPNGSLRRR RSRVLSEED EGNLMDFLR ASDNNRERR SWGSLDRSW ARKARGGSQ RKRPALLGA DFTSDRERP SSPSPLSES KPIVSTPEE ETNKPKELR QKIESWLQA SESDQTEDT RRQTRRITN RRSFEMDSE SERSSTLDT LPEGKQVYS GSQSTYRKA YPAWEPSKT IDNTDVVSA MEAVEEVQP QIKDKSAWR KSTLNVANS TEETKDDSM RLNRLPVVE NDRKTLISS LGERSPTDK LTLYIIKPR PRDSPPSLD DPPISNKVE IDQDNIETP PATRKIFIP TPTEKREPT QPSPCRRLM GRPRDTDEP EPNPTLGDG QFDRFSAAR RTRRYKRTD TDPDEPKDE PETRLKAWQ DKLQNPGRR HRHQTGINQ DEVKLALTK TDKKNEDEG FEESQSLMS ESPSQGASS GGNYEPEDS ALVVYAKPL KSEPPVEKP AKGRGLERG GVRRTVQDT PKKSVIPRR SGSLRKTNT STNVQRSSS RNSIVSSKS SLNSSASAC TVKKMPVKA GVGKAPPPK PVKRTPSTG GTAGTRPPR PLAMSFMKP TTSSATKVH TVPKNGAFR TK
- >TC011477 RB
MIAHNRTCP THTTKWKRP KSSEVKVSA STPSRSVRD AQLSAAPLF KSPPPPNHT TYARPKRKG CFLASAASR RPRKVPLEP TVRALFVNE SACAKGGKL HFSHVCRLG CIRNNRTMD SRIGLDFIV ENPEYIRKL AAALDTSNV TVKKQVFEL LSALCVHNE EGHARALDT LEHYKKLKG ERYRLNVIV QELSRATAV DYQTALVAF INCLIISTP RLTDRIRRR NEFIGCHLL PVLNNLRKC AEAEPELAV QLDVFDEQR DSDDSQSLQ GPHGVDLNS PLDVFYAIL KQVAETPQE IPFLSILQH LLRIDAKDA VSDIIWDTA ERLVHRATL LENREDAAR LLRAPSAQS KFFCHCHLR SESSPTRRP SLNTPQSPT PALPPPPPP PPNVPAVPP PVPAPPLKP AAPLPPPEC DSVIEKLPQ QEIPVPKIK MKTFNWNKI PNNKVIGKN NIWTQVAYT HQHSPMADL DWLEMEGLF CQQAPPANA AASFRRENT NSETLDRRI RKENCEITL LDGKRSLNV NIFLKQFRS SNEDIIHLI REGEHDDIG AEKLKGLLK ILPEVDELD MLKSFDGDF TKLGNAEKF LIQLTNLSN YKLRIESML LKEEFASNM SYLEPSIKS MIMAAQDLM TNKPLQEVL YMILVAGNF LNAGGYAGN AAGVKLSSL QKITDIRAN KPNMNLIHF VALQAEKKN PKLLTFTDN ISVLEDAAK TTVEQLQNE INVLDVRIK KIKKQIELA TTEPEIKEQ MTEFLQMAE REVSNLQQN MEELEKVRV QLAEFFCED TNSFKLEEC FRIFHGFYC KFKQAVGDN ERRRIQEEQ ANARRRQRE ELLASKRRQ MENLGGTPD SDVPFIDLQ IYDSRHGFP MKKGWKTTN GTSEDELSS VTGSPSLSR RRLGSFNGD PEKSPDITP NGSLRRRRS RVLSEEDEG NLMDFLRAS DNNRERRSW GSLDRSWAR KARGGSQRK RPALLGADF TSDRERPSS PSPLSESKP IVSTPEEET NKPKELRQK IESWLQASE SDQTEDTRR QTRRITNRR SFEMDSESE RSSTLDTLP EGKQVYSGS QSTYRKAYP AWEPSKTID NTDVVSAME AVEEVQPQI KDKSAWRKS TLNVANSTE ETKDDSMRL NRLPVVEND RKTLISSLG ERSPTDKLT LYIIKPRPR DSPPSLDDP PISNKVEID QDNIETPPA TRKIFIPTP TEKREPTQP SPCRRLMGR PRDTDEPEP NPTLGDGQF DRFSAARRT RRYKRTDTD PDEPKDEPE TRLKAWQDK LQNPGRRHR HQTGINQDE VKLALTKTD KKNEDEGFE ESQSLMSES PSQGASSGG NYEPEDSAL VVYAKPLKS EPPVEKPAK GRGLERGGV RRTVQDTPK KSVIPRRSG SLRKTNTST NVQRSSSRN SIVSSKSSL NSSASACTV KKMPVKAGV GKAPPPKPV KRTPSTGGT AGTRPPRPL AMSFMKPTT SSATKVHTV PKNGAFRTK
- >TC011477 RA
atggattcg aggatcggc ctggacttc atcgtggag aatcccgag tacatcagg aaactggcg gcggccctc gacaccagc aacgtcacc gtcaagaag caagtcttt gagcttctg tccgcgttg tgtgtgcac aacgaagag ggtcacgcg cgcgccctt gacactttg gagcactac aagaaattg aaaggtgag agatataga ctgaatgtg atcgtccag gagctgagc agggccacg gctgtggac taccagacg gcccttgtg gcctttatc aactgtctg atcatctcc acgcccagg ctcaccgac aggatcagg agacgcaac gaattcatt ggatgtcat ctgcttccg gtgctcaac aacttaaga aaatgcgcc gaggcggag cccgagctc gccgtccag ctggacgtg ttcgacgag cagcgcgac agcgacgac tcccagagc ctgcagggc ccccacggc gtggacctc aactcgccc ttggacgtc ttctacgcc atccttaaa caagttgcg gaaacgccg caggaaatc cccttcctg agcatccta cagcacctg ctgcgcatc gacgccaaa gacgccgtc agcgacatc atctgggac acggccgag cgtctcgtg caccgcgcc accctcctg gagaaccgc gaagacgcc gcccgcctg ctgcgcgcc cccagcgcc caatcaaag ttcttctgc cactgtcac ctccgctcc gagtctagt cccacccgc cgcccctcc ctcaacacg ccccagagc cccacgccc gcgctgccg ccgccgccg cccccgccc cccaacgtc cccgccgtc ccgcccccc gtccccgcg ccgcccctc aagcccgcc gcgccgctc ccgcccccc gaatgcgat tctgtgata gagaagtta ccccagcag gagatcccg gtgccgaag attaaaatg aagactttc aattggaat aaaataccg aataataag gtgattggg aagaataat atttggacg caagtggcg tacactcac cagcattcg ccgatggct gatttggat tggctggag atggagggg ctgttctgc caacaagcg cccccagcc aatgcggcg gcgagtttc aggcgcgaa aatacgaat tcagagact ttggatagg aggatacgc aaggaaaat tgcgagatt acgcttctg gacgggaag aggagtctc aatgttaat attttttta aaacagttt cggagctct aatgaggac atcatccac ttgatccgg gaaggggaa catgatgat atcggggcc gaaaaactc aaaggactc cttaaaata ctaccggaa gttgatgaa ctagacatg ctcaagtcg tttgatggc gatttcact aaattagga aacgccgaa aaattttta atacaactg accaactta tccaattat aaactacga atagaaagc atgcttctg aaagaagag tttgcctca aatatgagc tacttggag cctagcatt aaatcaatg attatggca gctcaagac ttgatgacg aacaaaccg cttcaagaa gtcttgtac atgattctg gtcgctggc aatttccta aatgcgggt ggctatgcc gggaacgcg gccggtgtt aaattgagt tcgttgcaa aaaatcaca gacattaga gcgaataaa ccaaatatg aatttaata cattttgtg gcgttgcaa gctgagaag aaaaacccc aaactgctg accttcacc gacaacata agcgtgctg gaggacgca gccaagacg acagtggag cagctccag aacgagatc aacgtgctc gacgtccgg ataaaaaaa atcaagaaa caaatcgaa ctggcaacc accgagccc gaaatcaaa gagcaaatg accgaattt ttgcaaatg gccgaacgt gaagtctcc aacttgcaa cagaacatg gaagagttg gagaaggtg cgtgtgcaa ctggctgaa tttttctgc gaggacacg aattcgttc aaacttgag gagtgtttc cggattttc cacgggttt tactgcaag ttcaagcaa gcagttggc gataacgag cgcaggagg atccaggag gagcaggcg aatgcgcgg cgccggcaa cgcgaggag ttgctcgct tccaagagg cggcaaatg gagaacttg gggggaacc cccgattcg gacgtgccg ttcatcgac ttgcaaatc tacgacagc aggcatggt tttccgatg aaaaaggga tggaaaacc accaacggg acatccgaa gacgagctt tcctcagtc accggctcc ccctctctc tcccggcgc cggctgggc tcatttaac ggcgacccg gagaaatcc cccgacatc acccctaat gggagcctg cggcggcgg cgcagccga gtcttgtcg gaggaggac gaaggcaac ttgatggac ttcctccgg gcttcggac aacaaccgc gagcgccgc tcctggggg agtctggac cggtcatgg gcccgcaaa gctagaggt gggagccag agaaaacgc ccggcttta ttaggtgct gacttcacc tcggaccgc gaacgcccc tcctcgccc tcacctcta agcgagtcc aaaccgatc gtttcaacc cccgaagaa gaaaccaac aaaccgaaa gaactgaga caaaaaatc gaatcctgg ctgcaggcc agcgaaagc gaccagacc gaggacacc cgacggcag actcgccgc atcacgaac cggcgctcc ttcgaaatg gactcggag agtgaacgc agcagcact ctggacacg ctgcccgaa ggcaagcaa gtctacagc ggcagccaa tccacctac cgcaaggcc tacccggcc tgggagccc tcgaagaca atcgacaac acggacgtg gtcagcgcc atggaagcc gtcgaggaa gtgcagccc caaatcaag gacaagtcg gcgtggcgc aaatccacc ctgaacgtg gccaacagc acggaagaa accaaagac gactcaatg cgtcttaac cgcctacca gtggtggag aacgaccgc aaaaccctg attagctct ctcggggag cgctccccc accgataaa ctcactctc tacattatc aagccacgc ccccgggac tcgcccccc agcctggac gacccaccc atcagcaac aaggtcgaa atcgaccaa gacaacata gaaacgccc ccagccacg cgcaaaatc ttcatcccc acgcccacc gagaagcgc gagccgacg cagcccagc ccctgtcgc cgcctcatg ggccgccca cgcgacacg gacgagccc gaacccaac ccgacgctc ggcgacggc caattcgac cggttttcc gccgcaagg cgcacacga cgctacaaa cgcaccgac accgacccc gacgaaccc aaagacgag cccgagacc cgcctcaag gcgtggcaa gacaagcta cagaacccc ggtaggcgc caccgccac caaaccgga atcaaccaa gacgaagtc aagctagcc ttgactaaa actgacaag aaaaacgaa gacgaaggc ttcgaggag agccagagt ttaatgtcg gagtcgccg agtcagggg gcgtcctct ggcggcaac tacgagccg gaggactcc gccctagtc gtctacgcc aaaccgctg aaaagcgag ccgccggtg gagaagccg gccaagggg cggggcctg gagcggggc ggagtcagg cgcacggtg caggacacg cccaaaaag agtgtgatt ccccgcagg tcggggagt ctgcgcaag accaacacc tcgacgaat gtgcaaagg tcgagttcg aggaatagt attgtcagt tcgaaaagt tcgctcaat agttcggcg tccgcttgc acggttaag aaaatgccg gttaaggcg ggtgttggc aaagccccg cccccgaaa ccggtcaag agaacgccc tcgactggg ggcacggcc gggacgaga cccccacga cccctggcc atgtctttc atgaaacct acgacttct agcgccact aaagtgcac acagtgccc aagaacggg gcctttaga acaaagtaa
- >TC011477 RB
atgatagcg cataataga acctgtccg acccacaca accaagtgg aagaggccg aagagctct gaagtgaaa gtgtcggcc tctactccg tcgaggtcc gtccgagat gcacagctg agtgcggcg ccactcttc aaatcgcca ccacctcca aaccacacg acgtacgcg cgcccgaaa cgcaagggg tgtttttta gcgtcggca gcgtcgcga cgtccacga aaagtgccg ctggagccg acggttcgc gcgctattt gtgaacgaa agtgcatgt gcaaaggga ggaaaattg cacttttcg catgtttgc cggcttgga tgtatccga aacaatcgg acgatggat tcgaggatc ggcctggac ttcatcgtg gagaatccc gagtacatc aggaaactg gcggcggcc ctcgacacc agcaacgtc accgtcaag aagcaagtc tttgagctt ctgtccgcg ttgtgtgtg cacaacgaa gagggtcac gcgcgcgcc cttgacact ttggagcac tacaagaaa ttgaaaggt gagagatat agactgaat gtgatcgtc caggagctg agcagggcc acggctgtg gactaccag acggccctt gtggccttt atcaactgt ctgatcatc tccacgccc aggctcacc gacaggatc aggagacgc aacgaattc attggatgt catctgctt ccggtgctc aacaactta agaaaatgc gccgaggcg gagcccgag ctcgccgtc cagctggac gtgttcgac gagcagcgc gacagcgac gactcccag agcctgcag ggcccccac ggcgtggac ctcaactcg cccttggac gtcttctac gccatcctt aaacaagtt gcggaaacg ccgcaggaa atccccttc ctgagcatc ctacagcac ctgctgcgc atcgacgcc aaagacgcc gtcagcgac atcatctgg gacacggcc gagcgtctc gtgcaccgc gccaccctc ctggagaac cgcgaagac gccgcccgc ctgctgcgc gcccccagc gcccaatca aagttcttc tgccactgt cacctccgc tccgagtct agtcccacc cgccgcccc tccctcaac acgccccag agccccacg cccgcgctg ccgccgccg ccgcccccg ccccccaac gtccccgcc gtcccgccc cccgtcccc gcgccgccc ctcaagccc gccgcgccg ctcccgccc cccgaatgc gattctgtg atagagaag ttaccccag caggagatc ccggtgccg aagattaaa atgaagact ttcaattgg aataaaata ccgaataat aaggtgatt gggaagaat aatatttgg acgcaagtg gcgtacact caccagcat tcgccgatg gctgatttg gattggctg gagatggag gggctgttc tgccaacaa gcgccccca gccaatgcg gcggcgagt ttcaggcgc gaaaatacg aattcagag actttggat aggaggata cgcaaggaa aattgcgag attacgctt ctggacggg aagaggagt ctcaatgtt aatattttt ttaaaacag tttcggagc tctaatgag gacatcatc cacttgatc cgggaaggg gaacatgat gatatcggg gccgaaaaa ctcaaagga ctccttaaa atactaccg gaagttgat gaactagac atgctcaag tcgtttgat ggcgatttc actaaatta ggaaacgcc gaaaaattt ttaatacaa ctgaccaac ttatccaat tataaacta cgaatagaa agcatgctt ctgaaagaa gagtttgcc tcaaatatg agctacttg gagcctagc attaaatca atgattatg gcagctcaa gacttgatg acgaacaaa ccgcttcaa gaagtcttg tacatgatt ctggtcgct ggcaatttc ctaaatgcg ggtggctat gccgggaac gcggccggt gttaaattg agttcgttg caaaaaatc acagacatt agagcgaat aaaccaaat atgaattta atacatttt gtggcgttg caagctgag aagaaaaac cccaaactg ctgaccttc accgacaac ataagcgtg ctggaggac gcagccaag acgacagtg gagcagctc cagaacgag atcaacgtg ctcgacgtc cggataaaa aaaatcaag aaacaaatc gaactggca accaccgag cccgaaatc aaagagcaa atgaccgaa tttttgcaa atggccgaa cgtgaagtc tccaacttg caacagaac atggaagag ttggagaag gtgcgtgtg caactggct gaatttttc tgcgaggac acgaattcg ttcaaactt gaggagtgt ttccggatt ttccacggg ttttactgc aagttcaag caagcagtt ggcgataac gagcgcagg aggatccag gaggagcag gcgaatgcg cggcgccgg caacgcgag gagttgctc gcttccaag aggcggcaa atggagaac ttgggggga acccccgat tcggacgtg ccgttcatc gacttgcaa atctacgac agcaggcat ggttttccg atgaaaaag ggatggaaa accaccaac gggacatcc gaagacgag ctttcctca gtcaccggc tccccctct ctctcccgg cgccggctg ggctcattt aacggcgac ccggagaaa tcccccgac atcacccct aatgggagc ctgcggcgg cggcgcagc cgagtcttg tcggaggag gacgaaggc aacttgatg gacttcctc cgggcttcg gacaacaac cgcgagcgc cgctcctgg gggagtctg gaccggtca tgggcccgc aaagctaga ggtgggagc cagagaaaa cgcccggct ttattaggt gctgacttc acctcggac cgcgaacgc ccctcctcg ccctcacct ctaagcgag tccaaaccg atcgtttca acccccgaa gaagaaacc aacaaaccg aaagaactg agacaaaaa atcgaatcc tggctgcag gccagcgaa agcgaccag accgaggac acccgacgg cagactcgc cgcatcacg aaccggcgc tccttcgaa atggactcg gagagtgaa cgcagcagc actctggac acgctgccc gaaggcaag caagtctac agcggcagc caatccacc taccgcaag gcctacccg gcctgggag ccctcgaag acaatcgac aacacggac gtggtcagc gccatggaa gccgtcgag gaagtgcag ccccaaatc aaggacaag tcggcgtgg cgcaaatcc accctgaac gtggccaac agcacggaa gaaaccaaa gacgactca atgcgtctt aaccgccta ccagtggtg gagaacgac cgcaaaacc ctgattagc tctctcggg gagcgctcc cccaccgat aaactcact ctctacatt atcaagcca cgcccccgg gactcgccc cccagcctg gacgaccca cccatcagc aacaaggtc gaaatcgac caagacaac atagaaacg cccccagcc acgcgcaaa atcttcatc cccacgccc accgagaag cgcgagccg acgcagccc agcccctgt cgccgcctc atgggccgc ccacgcgac acggacgag cccgaaccc aacccgacg ctcggcgac ggccaattc gaccggttt tccgccgca aggcgcaca cgacgctac aaacgcacc gacaccgac cccgacgaa cccaaagac gagcccgag acccgcctc aaggcgtgg caagacaag ctacagaac cccggtagg cgccaccgc caccaaacc ggaatcaac caagacgaa gtcaagcta gccttgact aaaactgac aagaaaaac gaagacgaa ggcttcgag gagagccag agtttaatg tcggagtcg ccgagtcag ggggcgtcc tctggcggc aactacgag ccggaggac tccgcccta gtcgtctac gccaaaccg ctgaaaagc gagccgccg gtggagaag ccggccaag gggcggggc ctggagcgg ggcggagtc aggcgcacg gtgcaggac acgcccaaa aagagtgtg attccccgc aggtcgggg agtctgcgc aagaccaac acctcgacg aatgtgcaa aggtcgagt tcgaggaat agtattgtc agttcgaaa agttcgctc aatagttcg gcgtccgct tgcacggtt aagaaaatg ccggttaag gcgggtgtt ggcaaagcc ccgcccccg aaaccggtc aagagaacg ccctcgact gggggcacg gccgggacg agaccccca cgacccctg gccatgtct ttcatgaaa cctacgact tctagcgcc actaaagtg cacacagtg cccaagaac ggggccttt agaacaaag taa
- >TC011477 RA
tccgaccca cacaaccaa gtggaagag gccgaagag ctctgaagt gaaagtgtc ggcctctac tccgtcgag gtccgtccg agatgcaca gctgagtgc ggcgccact cttcaaatc gccaccacc tccaaacca cacgacgta cgcgcgccc gaaacgcaa ggggtgttt tttagcgtc ggcagcgtc gcgacgtcc acgaaaagt gccgctgga gccgacggt tcgcgcgct atttgtgaa cgaaagtgc atgtgcaaa ggtgatgca aggattggg acattattc acgtgctgc tggggattg tcgctgatg ccgggacga cgataggga ggaaaattg cacttttcg catcaatcg gacgatgga ttcgaggat cggcctgga cttcatcgt ggagaatcc cgagtacat caggaaact ggcggcggc cctcgacac cagcaacgt caccgtcaa gaagcaagt ctttgagct tctgtccgc gttgtgtgt gcacaacga agagggtca cgcgcgcgc ccttgacac tttggagca ctacaagaa attgaaagg tgagagata tagactgaa tgtgatcgt ccaggagct gagcagggc cacggctgt ggactacca gacggccct tgtggcctt tatcaactg tctgatcat ctccacgcc caggctcac cgacaggat caggagacg caacgaatt cattggatg tcatctgct tccggtgct caacaactt aagaaaatg cgccgaggc ggagcccga gctcgccgt ccagctgga cgtgttcga cgagcagcg cgacagcga cgactccca gagcctgca gggccccca cggcgtgga cctcaactc gcccttgga cgtcttcta cgccatcct taaacaagt tgcggaaac gccgcagga aatcccctt cctgagcat cctacagca cctgctgcg catcgacgc caaagacgc cgtcagcga catcatctg ggacacggc cgagcgtct cgtgcaccg cgccaccct cctggagaa ccgcgaaga cgccgcccg cctgctgcg cgcccccag cgcccaatc aaagttctt ctgccactg tcacctccg ctccgagtc tagtcccac ccgccgccc ctccctcaa cacgcccca gagccccac gcccgcgct gccgccgcc gccgccccc gccccccaa cgtccccgc cgtcccgcc ccccgtccc cgcgccgcc cctcaagcc cgccgcgcc gctcccgcc ccccgaatg cgattctgt gatagagaa gttacccca gcaggagat cccggtgcc gaagattaa aatgaagac tttcaattg gaataaaat accgaataa taaggtgat tgggaagaa taatatttg gacgcaagt ggcgtacac tcaccagca ttcgccgat ggctgattt ggattggct ggagatgga ggggctgtt ctgccaaca agcgccccc agccaatgc ggcggcgag tttcaggcg cgaaaatac gaattcaga gactttgga taggaggat acgcaagga aaattgcga gattacgct tctggacgg gaagaggag tctcaatgt taatatttt tttaaaaca gtttcggag ctctaatga ggacatcat ccacttgat ccgggaagg ggaacatga tgatatcgg ggccgaaaa actcaaagg actccttaa aatactacc ggaagttga tgaactaga catgctcaa gtcgtttga tggcgattt cactaaatt aggaaacgc cgaaaaatt tttaataca actgaccaa cttatccaa ttataaact acgaataga aagcatgct tctgaaaga agagtttgc ctcaaatat gagctactt ggagcctag cattaaatc aatgattat ggcagctca agacttgat gacgaacaa accgcttca agaagtctt gtacatgat tctggtcgc tggcaattt cctaaatgc gggtggcta tgccgggaa cgcggccgg tgttaaatt gagttcgtt gcaaaaaat cacagacat tagagcgaa taaaccaaa tatgaattt aatacattt tgtggcgtt gcaagctga gaagaaaaa ccccaaact gctgacctt caccgacaa cataagcgt gctggagga cgcagccaa gacgacagt ggagcagct ccagaacga gatcaacgt gctcgacgt ccggataaa aaaaatcaa gaaacaaat cgaactggc aaccaccga gcccgaaat caaagagca aatgaccga atttttgca aatggccga acgtgaagt ctccaactt gcaacagaa catggaaga gttggagaa ggtgcgtgt gcaactggc tgaattttt ctgcgagga cacgaattc gttcaaact tgaggagtg tttccggat tttccacgg gttttactg caagttcaa gcaagcagt tggcgataa cgagcgcag gaggatcca ggaggagca ggcgaatgc gcggcgccg gcaacgcga ggagttgct cgcttccaa gaggcggca aatggagaa cttgggggg aacccccga ttcggacgt gccgttcat cgacttgca aatctacga cagcaggca tggttttcc gatgaaaaa gggatggaa aaccaccaa cgggacatc cgaagacga gctttcctc agtcaccgg ctccccctc tctctcccg gcgccggct gggctcatt taacggcga cccggagaa atcccccga catcacccc taatgggag cctgcggcg gcggcgcag ccgagtctt gtcggagga ggacgaagg caacttgat ggacttcct ccgggcttc ggacaacaa ccgcgagcg ccgctcctg ggggagtct ggaccggtc atgggcccg caaagctag aggtgggag ccagagaaa acgcccggc tttattagg tgctgactt cacctcgga ccgcgaacg cccctcctc gccctcacc tctaagcga gtccaaacc gatcgtttc aacccccga agaagaaac caacaaacc gaaagaact gagacaaaa aatcgaatc ctggctgca ggccagcga aagcgacca gaccgagga cacccgacg gcagactcg ccgcatcac gaaccggcg ctccttcga aatggactc ggagagtga acgcagcag cactctgga cacgctgcc cgaaggcaa gcaagtcta cagcggcag ccaatccac ctaccgcaa ggcctaccc ggcctggga gccctcgaa gacaatcga caacacgga cgtggtcag cgccatgga agccgtcga ggaagtgca gccccaaat caaggacaa gtcggcgtg gcgcaaatc caccctgaa cgtggccaa cagcacgga agaaaccaa agacgactc aatgcgtct taaccgcct accagtggt ggagaacga ccgcaaaac cctgattag ctctctcgg ggagcgctc ccccaccga taaactcac tctctacat tatcaagcc acgcccccg ggactcgcc ccccagcct ggacgaccc acccatcag caacaaggt cgaaatcga ccaagacaa catagaaac gcccccagc cacgcgcaa aatcttcat ccccacgcc caccgagaa gcgcgagcc gacgcagcc cagcccctg tcgccgcct catgggccg cccacgcga cacggacga gcccgaacc caacccgac gctcggcga cggccaatt cgaccggtt ttccgccgc aaggcgcac acgacgcta caaacgcac cgacaccga ccccgacga acccaaaga cgagcccga gacccgcct caaggcgtg gcaagacaa gctacagaa ccccggtag gcgccaccg ccaccaaac cggaatcaa ccaagacga agtcaagct agccttgac taaaactga caagaaaaa cgaagacga aggcttcga ggagagcca gagtttaat gtcggagtc gccgagtca gggggcgtc ctctggcgg caactacga gccggagga ctccgccct agtcgtcta cgccaaacc gctgaaaag cgagccgcc ggtggagaa gccggccaa ggggcgggg cctggagcg gggcggagt caggcgcac ggtgcagga cacgcccaa aaagagtgt gattccccg caggtcggg gagtctgcg caagaccaa cacctcgac gaatgtgca aaggtcgag ttcgaggaa tagtattgt cagttcgaa aagttcgct caatagttc ggcgtccgc ttgcacggt taagaaaat gccggttaa ggcgggtgt tggcaaagc cccgccccc gaaaccggt caagagaac gccctcgac tgggggcac ggccgggac gagaccccc acgacccct ggccatgtc tttcatgaa acctacgac ttctagcgc cactaaagt gcacacagt gcccaagaa cggggcctt tagaacaaa gtaatcact cattttttc actagtcaa ggattatta ttacgaact aaatctttt attcctac
- >TC011477 RB
ctgtaccag gagcggtct agttatccg ctaaccgtc tatgatagc gcataatag aacctgtcc gacccacac aaccaagtg gaagaggcc gaagagctc tgaagtgaa agtgtcggc ctctactcc gtcgaggtc cgtccgaga tgcacagct gagtgcggc gccactctt caaatcgcc accacctcc aaaccacac gacgtacgc gcgcccgaa acgcaaggg gtgtttttt agcgtcggc agcgtcgcg acgtccacg aaaagtgcc gctggagcc gacggttcg cgcgctatt tgtgaacga aagtgcatg tgcaaaggg aggaaaatt gcacttttc gcatgtttg ccggcttgg atgtatccg aaacaatcg gacgatgga ttcgaggat cggcctgga cttcatcgt ggagaatcc cgagtacat caggaaact ggcggcggc cctcgacac cagcaacgt caccgtcaa gaagcaagt ctttgagct tctgtccgc gttgtgtgt gcacaacga agagggtca cgcgcgcgc ccttgacac tttggagca ctacaagaa attgaaagg tgagagata tagactgaa tgtgatcgt ccaggagct gagcagggc cacggctgt ggactacca gacggccct tgtggcctt tatcaactg tctgatcat ctccacgcc caggctcac cgacaggat caggagacg caacgaatt cattggatg tcatctgct tccggtgct caacaactt aagaaaatg cgccgaggc ggagcccga gctcgccgt ccagctgga cgtgttcga cgagcagcg cgacagcga cgactccca gagcctgca gggccccca cggcgtgga cctcaactc gcccttgga cgtcttcta cgccatcct taaacaagt tgcggaaac gccgcagga aatcccctt cctgagcat cctacagca cctgctgcg catcgacgc caaagacgc cgtcagcga catcatctg ggacacggc cgagcgtct cgtgcaccg cgccaccct cctggagaa ccgcgaaga cgccgcccg cctgctgcg cgcccccag cgcccaatc aaagttctt ctgccactg tcacctccg ctccgagtc tagtcccac ccgccgccc ctccctcaa cacgcccca gagccccac gcccgcgct gccgccgcc gccgccccc gccccccaa cgtccccgc cgtcccgcc ccccgtccc cgcgccgcc cctcaagcc cgccgcgcc gctcccgcc ccccgaatg cgattctgt gatagagaa gttacccca gcaggagat cccggtgcc gaagattaa aatgaagac tttcaattg gaataaaat accgaataa taaggtgat tgggaagaa taatatttg gacgcaagt ggcgtacac tcaccagca ttcgccgat ggctgattt ggattggct ggagatgga ggggctgtt ctgccaaca agcgccccc agccaatgc ggcggcgag tttcaggcg cgaaaatac gaattcaga gactttgga taggaggat acgcaagga aaattgcga gattacgct tctggacgg gaagaggag tctcaatgt taatatttt tttaaaaca gtttcggag ctctaatga ggacatcat ccacttgat ccgggaagg ggaacatga tgatatcgg ggccgaaaa actcaaagg actccttaa aatactacc ggaagttga tgaactaga catgctcaa gtcgtttga tggcgattt cactaaatt aggaaacgc cgaaaaatt tttaataca actgaccaa cttatccaa ttataaact acgaataga aagcatgct tctgaaaga agagtttgc ctcaaatat gagctactt ggagcctag cattaaatc aatgattat ggcagctca agacttgat gacgaacaa accgcttca agaagtctt gtacatgat tctggtcgc tggcaattt cctaaatgc gggtggcta tgccgggaa cgcggccgg tgttaaatt gagttcgtt gcaaaaaat cacagacat tagagcgaa taaaccaaa tatgaattt aatacattt tgtggcgtt gcaagctga gaagaaaaa ccccaaact gctgacctt caccgacaa cataagcgt gctggagga cgcagccaa gacgacagt ggagcagct ccagaacga gatcaacgt gctcgacgt ccggataaa aaaaatcaa gaaacaaat cgaactggc aaccaccga gcccgaaat caaagagca aatgaccga atttttgca aatggccga acgtgaagt ctccaactt gcaacagaa catggaaga gttggagaa ggtgcgtgt gcaactggc tgaattttt ctgcgagga cacgaattc gttcaaact tgaggagtg tttccggat tttccacgg gttttactg caagttcaa gcaagcagt tggcgataa cgagcgcag gaggatcca ggaggagca ggcgaatgc gcggcgccg gcaacgcga ggagttgct cgcttccaa gaggcggca aatggagaa cttgggggg aacccccga ttcggacgt gccgttcat cgacttgca aatctacga cagcaggca tggttttcc gatgaaaaa gggatggaa aaccaccaa cgggacatc cgaagacga gctttcctc agtcaccgg ctccccctc tctctcccg gcgccggct gggctcatt taacggcga cccggagaa atcccccga catcacccc taatgggag cctgcggcg gcggcgcag ccgagtctt gtcggagga ggacgaagg caacttgat ggacttcct ccgggcttc ggacaacaa ccgcgagcg ccgctcctg ggggagtct ggaccggtc atgggcccg caaagctag aggtgggag ccagagaaa acgcccggc tttattagg tgctgactt cacctcgga ccgcgaacg cccctcctc gccctcacc tctaagcga gtccaaacc gatcgtttc aacccccga agaagaaac caacaaacc gaaagaact gagacaaaa aatcgaatc ctggctgca ggccagcga aagcgacca gaccgagga cacccgacg gcagactcg ccgcatcac gaaccggcg ctccttcga aatggactc ggagagtga acgcagcag cactctgga cacgctgcc cgaaggcaa gcaagtcta cagcggcag ccaatccac ctaccgcaa ggcctaccc ggcctggga gccctcgaa gacaatcga caacacgga cgtggtcag cgccatgga agccgtcga ggaagtgca gccccaaat caaggacaa gtcggcgtg gcgcaaatc caccctgaa cgtggccaa cagcacgga agaaaccaa agacgactc aatgcgtct taaccgcct accagtggt ggagaacga ccgcaaaac cctgattag ctctctcgg ggagcgctc ccccaccga taaactcac tctctacat tatcaagcc acgcccccg ggactcgcc ccccagcct ggacgaccc acccatcag caacaaggt cgaaatcga ccaagacaa catagaaac gcccccagc cacgcgcaa aatcttcat ccccacgcc caccgagaa gcgcgagcc gacgcagcc cagcccctg tcgccgcct catgggccg cccacgcga cacggacga gcccgaacc caacccgac gctcggcga cggccaatt cgaccggtt ttccgccgc aaggcgcac acgacgcta caaacgcac cgacaccga ccccgacga acccaaaga cgagcccga gacccgcct caaggcgtg gcaagacaa gctacagaa ccccggtag gcgccaccg ccaccaaac cggaatcaa ccaagacga agtcaagct agccttgac taaaactga caagaaaaa cgaagacga aggcttcga ggagagcca gagtttaat gtcggagtc gccgagtca gggggcgtc ctctggcgg caactacga gccggagga ctccgccct agtcgtcta cgccaaacc gctgaaaag cgagccgcc ggtggagaa gccggccaa ggggcgggg cctggagcg gggcggagt caggcgcac ggtgcagga cacgcccaa aaagagtgt gattccccg caggtcggg gagtctgcg caagaccaa cacctcgac gaatgtgca aaggtcgag ttcgaggaa tagtattgt cagttcgaa aagttcgct caatagttc ggcgtccgc ttgcacggt taagaaaat gccggttaa ggcgggtgt tggcaaagc cccgccccc gaaaccggt caagagaac gccctcgac tgggggcac ggccgggac gagaccccc acgacccct ggccatgtc tttcatgaa acctacgac ttctagcgc cactaaagt gcacacagt gcccaagaa cggggcctt tagaacaaa gtaatcact cattttttc actagtcaa ggattatta ttacgaact aaatctttt attcctac
