Status
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Gene information
General information
TC011462 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 6587920..6593199
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011462
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05040

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011462 RA
      MVEEEPKIQ AIEGVTSPT IVGLCLGGG VFVLCMAAL 
      TCFCYRQRR SHSAKRHGP DQPLAFHAH RRPTAVKSP 
      AGATTHYLK KSPSPTGPS KTPPGMSAP HTPSPTGSS 
      SGSMAGPLE SGVANDKVD HSATMKDVY TTENEVHTP 
      TKEEIETNE KNLQQSAAY LGQLVFKLR YKHDKNALI 
      VSVVRCRDL PAKDPNLGS SDPYVKLQL LPDKQHKVK 
      TRVLRKTRN PVYDEDFTF YGINFNQLP NITLHFVVL 
      SFDRYSRDD IIGEVFCAL NTVDVTQIE NQQMALCRE 
      IQPRSLKIR SQGRGELLV SLCWQPAAN RLTVVVLKA 
      RNLPKMDVT GLADPYVKI YLLYNGQRI AKKKTHVKK 
      RTLSPVFNE SFVFDIPAG GEGLENVSL EFLLLDWDR 
      VTKNEVIGR LEMGGAKCT GTALHHWNE VCNSARRQI 
      ADWHKLRE
    CDS sequence
    • >TC011462 RA
      atggtggag gaagaacca aagatccag gccatcgaa 
      ggagtgacc tcgccgacc atcgtgggg ctgtgtctg 
      ggcggtggg gtgtttgta ttgtgcatg gccgccttg 
      acgtgcttc tgctaccgg cagcggagg agccacagc 
      gccaagcgc catgggccc gaccagccg ctggccttc 
      cacgcccat cgcaggccc acggcggtc aagagtcca 
      gccggggcc acgacgcac tacctgaag aagagcccc 
      agtcccacg ggaccgtcc aagacgccg ccggggatg 
      tcggcaccg catacgccg agtccaaca ggtagttct 
      tcggggagt atggcaggg ccgctggag agtggggtg 
      gctaatgac aaggtggat cacagtgcc acgatgaag 
      gatgtgtac acgaccgag aatgaggtg catacgccc 
      accaaggag gaaattgag acgaatgag aaaaatctg 
      cagcagagt gcggcgtat ctgggacag ctcgtgttc 
      aagctcaga tacaaacac gataagaac gcccttatc 
      gtgtcggtg gtccggtgc cgcgacctc cccgccaaa 
      gaccccaac ctgggctcc agcgacccc tacgtcaaa 
      ctgcagctc ctccccgac aaacaacac aaagtcaaa 
      acccgcgtt ttgcgcaaa actcgcaat cccgtctac 
      gacgaagac ttcactttc tacggaatc aacttcaac 
      caactcccc aacatcacc ctccacttc gtcgtcctc 
      agcttcgac cgctacagc cgtgacgac atcattggc 
      gaggtcttc tgtgctctg aacaccgtc gacgtcacc 
      caaatcgaa aaccaacaa atggctcta tgccgagaa 
      atccaaccg cgaagcctc aagatccgc tcgcaaggg 
      cgtggcgaa cttctcgtt tcgttgtgt tggcaaccg 
      gcagccaat cgcctcaca gtcgtggtt ctaaaagct 
      cggaacttg cctaagatg gacgtgacg ggattggct 
      gatccttac gtcaagatt tatttgctg tacaatgga 
      caacggatt gccaaaaag aagacacat gtcaagaaa 
      cggacgctg agtccggtg ttcaacgaa agtttcgtt 
      tttgatatt ccagcagga ggggagggt ttggagaac 
      gtcagtttg gagtttttg ctcctggat tgggacagg 
      gtgaccaaa aatgaggtg attggtcga ctggaaatg 
      ggcggggcc aagtgcact ggaacggcg ctccaccat 
      tggaacgag gtgtgcaac tcggctcgc cgacaaatc 
      gccgattgg cacaaactt agggagtag
    mRNA sequence
    • >TC011462 RA
      taagtcgaa atatccatt ggaacggca ttaaaattt 
      cactctcgc ggaagcgac cagaattaa tcattgagg 
      gtcgagccc cgctctaag tgcaccatg gtggaggaa 
      gaaccaaag atccaggcc atcgaagga gtgacctcg 
      ccgaccatc gtggggctg tgtctgggc ggtggggtg 
      tttgtattg tgcatggcc gccttgacg tgcttctgc 
      taccggcag cggaggagc cacagcgcc aagcgccat 
      gggcccgac cagccgctg gccttccac gcccatcgc 
      aggcccacg gcggtcaag agtccagcc ggggccacg 
      acgcactac ctgaagaag agccccagt cccacggga 
      ccgtccaag acgccgccg gggatgtcg gcaccgcat 
      acgccgagt ccaacaggt agttcttcg gggagtatg 
      gcagggccg ctggagagt ggggtggct aatgacaag 
      gtggatcac agtgccacg atgaaggat gtgtacacg 
      accgagaat gaggtgcat acgcccacc aaggaggaa 
      attgagacg aatgagaaa aatctgcag cagagtgcg 
      gcgtatctg ggacagctc gtgttcaag ctcagatac 
      aaacacgat aagaacgcc cttatcgtg tcggtggtc 
      cggtgccgc gacctcccc gccaaagac cccaacctg 
      ggctccagc gacccctac gtcaaactg cagctcctc 
      cccgacaaa caacacaaa gtcaaaacc cgcgttttg 
      cgcaaaact cgcaatccc gtctacgac gaagacttc 
      actttctac ggaatcaac ttcaaccaa ctccccaac 
      atcaccctc cacttcgtc gtcctcagc ttcgaccgc 
      tacagccgt gacgacatc attggcgag gtcttctgt 
      gctctgaac accgtcgac gtcacccaa atcgaaaac 
      caacaaatg gctctatgc cgagaaatc caaccgcga 
      agcctcaag atccgctcg caagggcgt ggcgaactt 
      ctcgtttcg ttgtgttgg caaccggca gccaatcgc 
      ctcacagtc gtggttcta aaagctcgg aacttgcct 
      aagatggac gtgacggga ttggctgat ccttacgtc 
      aagatttat ttgctgtac aatggacaa cggattgcc 
      aaaaagaag acacatgtc aagaaacgg acgctgagt 
      ccggtgttc aacgaaagt ttcgttttt gatattcca 
      gcaggaggg gagggtttg gagaacgtc agtttggag 
      tttttgctc ctggattgg gacagggtg accaaaaat 
      gaggtgatt ggtcgactg gaaatgggc ggggccaag 
      tgcactgga acggcgctc caccattgg aacgaggtg 
      tgcaactcg gctcgccga caaatcgcc gattggcac 
      aaacttagg gagtaggcg ggtgattgg ttctgaatg 
      gctggtttg aatttggcg cttgtggat tagccaata 
      ggtaactaa ttgtgtaat tgtttcacc gcaaacatt 
      gtaggcttt gttttgttg tcgcttagg ttttatgta 
      aatatgaaa caatagatg ttaggcttc tggtttttt 
      ttagaatat tattagtaa atctttccc tatttaaat 
      gacgagtaa agcaatcgg gcagggtcg cgcttaaca 
      agacttaaa attgagatt agctttgtt acaaaaggg 
      tttgtgata tagatttaa gctgattaa aaagtacca 
      aaaaatgta atctaaatg tatcataac tacgcactt 
      tagagcaag ttgcacgct actggcgta gggatccat 
      cactggata ttttatttc tcaaaaaat acatttggg 
      gattttcgg aacacactc ctggatttt gggccattt 
      taccacaac ttttatcaa tagacaacg aagtttcta 
      cgcacatac tctcaagac atactcaaa aaatccgca 
      aatgtatta acgcaccta caaatacta tagtaaagt 
      aatacgatg taaatttcc cggatggct ttaggacgc 
      ccctcctct tgtgacggt gggcggagc gaactaaac 
      tcgccccac ccctactct tacttatat aggacacac 
      ggtagtgac gtattagac ttaatattc agctctaaa 
      tgcgtactt aatccaatt atttgccgg ttgatatac 
      agtatgttc acactgttg gttacttta ccaatgcac 
      actgtatat attgatcac gtgacagtt attttatat 
      ttgctttcc aagatactc tcggatctc ttatactcg 
      gttccagaa attatgaat gtatataat aaatgaata 
      aatgtttca atggttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY