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Gene information
General information
TC011457 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 6384383..6388707
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011457
    iBeetle screen iB_05039

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    adult nutritional state - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: 2 - Number of animals showing the phenotype: 2
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature potentially fluorescence not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011457 RA
      MSDKSPETS LMSEGGPNV GPQGGPNEA KDPPDAVDI 
      NENQSIEIT NKVLEQAEE IAQLTQKLS LVESKLDLA 
      SKQKELAQK EKEAMVIKY AVSEKNLLD MKHQKEQIE 
      KKYKEQVNE NEIIQHKVQ VMGSEKSRI CQMLDNKCY 
      EFRGCQQEL EQAKADLSA LETKLKWSQ NSLKTEVQL 
      HKETQSRLD EMTKKCQDA SDQIEQAKK DAQESIKSF 
      HSSQDNRAH VLDQQLKEL QAALILLKH EKEDKEQQI 
      KSLHSQLEK LQTKQKDML QENNALSLK VQHLERDRL 
      ESEQKLSDF KACADQQRQ DCADLQAKT ALLDQLKLQ 
      LKNEQEQNK ACNEQISLL KQRNAELEA DILACRERE 
      AELLLFTQQ LTEKNVRLQ SEFTALETK VQQLSCEQT 
      ILKRSLKEQ ETKNAVVSA QMTAERAKL SAEVEGLRV 
      ELDESRGLC ERLKQEVGD HKGENKLLK RKCELSLKE 
      VQKELQQCR KRLEQYEVE NQSSSSSNS SLSVGSPAN 
      EQVKVIQPD PGLDRQTLI EHIVKLQRI SARKSEKLD 
      FLEEHVNTL VLELQKKSR LLQSYILRD QSGTLTSNT 
      MDNNKANIA KLNGVMASV YGSRVADDK LTLELSLDI 
      NRKLQAVLE DALLKNITL KDNVDTLGQ EIDRLNKLL 
      KT
    CDS sequence
    • >TC011457 RA
      atgagtgat aagtctccg gagacctca ctcatgtca 
      gaaggtggc cccaatgtg ggccctcaa gggggtcca 
      aatgaagct aaagaccca ccggacgct gttgacatc 
      aacgagaat caatcaatc gaaatcaca aacaaggta 
      ttagaacag gctgaagaa atcgcacag ctgacccag 
      aaattgtct ctcgtggag tccaaacta gacctagca 
      agcaagcag aaagaattg gcccagaag gagaaggaa 
      gcaatggtg atcaagtat gccgttagt gagaagaac 
      ttgcttgat atgaagcac cagaaggag caaatcgag 
      aaaaaatac aaagagcaa gtcaacgag aatgaaata 
      atccagcac aaggtccaa gtgatgggc tcagagaag 
      tcccgaatt tgccaaatg ctcgataac aagtgttac 
      gagttcagg ggctgccaa caggagctg gagcaagcc 
      aaggccgac ttgagcgcc ctggagacg aagctcaag 
      tggtcccaa aacagcctc aagacggag gtccagctc 
      cacaaagaa acccagagc cgtcttgac gaaatgaca 
      aaaaaatgc caggacgcc tccgatcag atagaacag 
      gcgaaaaaa gacgcgcaa gaatcgatc aaatcgttt 
      cacagctcc caggacaac cgagctcac gtccttgac 
      caacaactg aaggagctc caagctgct ctgatcctc 
      ctcaagcac gagaaagaa gacaaggag caacaaatc 
      aaatccctc cactcccag ctggagaag ctccaaacg 
      aagcagaaa gacatgctg caggagaac aacgctctc 
      tccctcaaa gtgcagcac ttggagcgc gatcgcctg 
      gaaagcgag cagaaactg agcgatttc aaggcctgt 
      gccgaccag cagcgccaa gactgcgcc gacttacaa 
      gcaaaaacg gccctctta gaccaatta aaactacag 
      ttaaagaac gaacaagag cagaataaa gcttgtaac 
      gagcaaatc agtctttta aagcagcgc aacgccgag 
      ctcgaggcc gatatcctg gcttgtaga gagcgcgag 
      gctgaactg ttgctcttc acgcaacag ttgaccgag 
      aagaatgtg cgattacag tcggagttt acggcgctg 
      gagaccaaa gtccagcag ttgagttgc gagcaaacg 
      atactcaag cgctcgctg aaggagcaa gagacgaaa 
      aatgcggtg gtttcggcg caaatgacc gcagaaagg 
      gccaaattg agtgcggaa gtggagggg ttgagggtg 
      gagttggac gagagtcgg ggtttgtgc gagaggttg 
      aagcaggag gtgggggat cataagggc gaaaataag 
      ttgctgaag cggaagtgc gagttgtcg ctgaaggag 
      gtgcagaag gagttgcaa cagtgtcgg aaaaggctg 
      gaacagtac gaggtggag aaccagagt agtagtagc 
      tcgaattcg tcgcttagt gtggggtcg ccggccaac 
      gagcaagtt aaggttatc cagcctgac cccggcctg 
      gaccggcaa accctcatc gagcacata gtcaaactg 
      caacgaatc agtgcgcga aaatcggaa aagctcgat 
      tttttggaa gaacacgtc aacactcta gttttagaa 
      ttgcaaaaa aaatcgcgt cttttacaa agttacatc 
      ttaagagac cagtcaggc actctaact tctaacact 
      atggataac aataaagcg aacatcgcc aaattgaac 
      ggtgttatg gcatcggtg tatggctcg cgtgtagct 
      gatgataaa ctcacgctg gagttgtcc ctcgacatc 
      aatcggaag ctacaagct gtactcgag gatgccttg 
      cttaaaaat attactttg aaggacaat gtggacacg 
      ttggggcag gaaatcgac cgcttaaat aaattgttg 
      aagacgtaa
    mRNA sequence
    • >TC011457 RA
      gtctagttg tgttggtaa atcgaagtg ttaaatatg 
      tttaattag aatattatt acaaattct attgatcgg 
      gaacaattt aaacagttt aaaatgtga ttttggtca 
      taattctaa acttacaat gagtgataa gtctccgga 
      gacctcact catgtcaga aggtggccc caatgtggg 
      ccctcaagg gggtccaaa tgaagctaa agacccacc 
      ggacgctgt tgacatcaa cgagaatca atcaatcga 
      aatcacaaa caaggtatt agaacaggc tgaagaaat 
      cgcacagct gacccagaa attgtctct cgtggagtc 
      caaactaga cctagcaag caagcagaa agaattggc 
      ccagaagga gaaggaagc aatggtgat caagtatgc 
      cgttagtga gaagaactt gcttgatat gaagcacca 
      gaaggagca aatcgagaa aaaatacaa agagcaagt 
      caacgagaa tgaaataat ccagcacaa ggtccaagt 
      gatgggctc agagaagtc ccgaatttg ccaaatgct 
      cgataacaa gtgttacga gttcagggg ctgccaaca 
      ggagctgga gcaagccaa ggccgactt gagcgccct 
      ggagacgaa gctcaagtg gtcccaaaa cagcctcaa 
      gacggaggt ccagctcca caaagaaac ccagagccg 
      tcttgacga aatgacaaa aaaatgcca ggacgcctc 
      cgatcagat agaacaggc gaaaaaaga cgcgcaaga 
      atcgatcaa atcgtttca cagctccca ggacaaccg 
      agctcacgt ccttgacca acaactgaa ggagctcca 
      agctgctct gatcctcct caagcacga gaaagaaga 
      caaggagca acaaatcaa atccctcca ctcccagct 
      ggagaagct ccaaacgaa gcagaaaga catgctgca 
      ggagaacaa cgctctctc cctcaaagt gcagcactt 
      ggagcgcga tcgcctgga aagcgagca gaaactgag 
      cgatttcaa ggcctgtgc cgaccagca gcgccaaga 
      ctgcgccga cttacaagc aaaaacggc cctcttaga 
      ccaattaaa actacagtt aaagaacga acaagagca 
      gaataaagc ttgtaacga gcaaatcag tcttttaaa 
      gcagcgcaa cgccgagct cgaggccga tatcctggc 
      ttgtagaga gcgcgaggc tgaactgtt gctcttcac 
      gcaacagtt gaccgagaa gaatgtgcg attacagtc 
      ggagtttac ggcgctgga gaccaaagt ccagcagtt 
      gagttgcga gcaaacgat actcaagcg ctcgctgaa 
      ggagcaaga gacgaaaaa tgcggtggt ttcggcgca 
      aatgaccgc agaaagggc caaattgag tgcggaagt 
      ggaggggtt gagggtgga gttggacga gagtcgggg 
      tttgtgcga gaggttgaa gcaggaggt gggggatca 
      taagggcga aaataagtt gctgaagcg gaagtgcga 
      gttgtcgct gaaggaggt gcagaagga gttgcaaca 
      gtgtcggaa aaggctgga acagtacga ggtggagaa 
      ccagagtag tagtagctc gaattcgtc gcttagtgt 
      ggggtcgcc ggccaacga gcaagttaa ggttatcca 
      gcctgaccc cggcctgga ccggcaaac cctcatcga 
      gcacatagt caaactgca acgaatcag tgcgcgaaa 
      atcggaaaa gctcgattt tttggaaga acacgtcaa 
      cactctagt tttagaatt gcaaaaaaa atcgcgtct 
      tttacaaag ttacatctt aagagacca gtcaggcac 
      tctaacttc taacactat ggataacaa taaagcgaa 
      catcgccaa attgaacgg tgttatggc atcggtgta 
      tggctcgcg tgtagctga tgataaact cacgctgga 
      gttgtccct cgacatcaa tcggaagct acaagctgt 
      actcgagga tgccttgct taaaaatat tactttgaa 
      ggacaatgt ggacacgtt ggggcagga aatcgaccg 
      cttaaataa attgttgaa gacgtaatg cggtcgcac 
      cttgtacat aaatcaagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY