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Gene information
General information
TC011451 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 6213725..6222249
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011451
    iBeetle screen iB_05038

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011451 RB
      MVYDCGSCL AKYILCAFN FIIFLAGSV VLAVGIWLA 
      VDKNSFVGL SKIVPSDHV QQFIQPAVI EQGAYILIA 
      VGGFMFIVS FLGYCGALR ESQCLLTTY GICLLVILI 
      AEIAVACLT VAYKPKAEE ETRKLLKST IENYYSPPE 
      NKDAVTLMW DHLQANLKC CGVNNYTDY RSSTKWTNS 
      GKVIPESCC VLEGDPLKL KPKVPSCVT SPNEVNSYY 
      KQGCFDALV HWIMIHVDV VIGVAVGLG LLELVGIFL 
      AFCLAKALN GYIK
    CDS sequence
    • >TC011451 RB
      atggtctac gattgtggc tcctgtttg gccaagtac 
      attctgtgc gcgttcaat tttatcata tttttagct 
      ggctctgtg gtcctagct gttggcatc tggctggcc 
      gtggataaa aactcgttc gtaggactg agcaaaatc 
      gttcccagc gaccatgtc cagcaattt atccaacct 
      gccgtcatc gaacaaggg gcctatatt ctgatcgcc 
      gtgggcggt ttcatgttt atcgtcagt tttttgggc 
      tattgtggc gccttaaga gagtcccaa tgcctccta 
      acgacgtac ggcatttgt ttgttggtg attttgatc 
      gccgaaatc gcggtggct tgtctgaca gtggcgtac 
      aagccgaag gcggaggag gaaactcga aaattactc 
      aaatcaacg attgaaaat tactactca ccgccggaa 
      aataaagac gccgtgacg ttgatgtgg gaccacctc 
      caggccaac ttgaaatgt tgcggcgtt aataattac 
      accgattat cggagcagc acgaagtgg acaaacagt 
      gggaaagtg attcctgag tcgtgctgc gtcctcgaa 
      ggggatccc ctgaagttg aagccgaag gttccgtcg 
      tgtgtgacg agtccgaac gaagtcaac tcgtactac 
      aaacaaggt tgcttcgat gcgctcgtc cactggatc 
      atgatccac gttgatgtg gtcattggt gtggctgtc 
      ggtttgggt ttgctcgag ttggtcggg atatttctg 
      gcgttttgt ttagctaag gccctcaat gggtacata 
      aag
    mRNA sequence
    • >TC011451 RB
      ttgttgttg agttagtcg tgttgttgg tgttgtgcg 
      ttcgctcgt ctccaattc tggaattaa ctaaacatg 
      agataaagc agtcgaacg acttgattg gggattcaa 
      ttaattgat gctgtagat ggaaaggag gtgaaagaa 
      caaccaacc gcaaaatgg tctacgatt gtggctcct 
      gtttggcca agtacattc tgtgcgcgt tcaatttta 
      tcatatttt tagctggct ctgtggtcc tagctgttg 
      gcatctggc tggccgtgg ataaaaact cgttcgtag 
      gactgagca aaatcgttc ccagcgacc atgtccagc 
      aatttatcc aacctgccg tcatcgaac aaggggcct 
      atattctga tcgccgtgg gcggtttca tgtttatcg 
      tcagttttt tgggctatt gtggcgcct taagagagt 
      cccaatgcc tcctaacga cgtacggca tttgtttgt 
      tggtgattt tgatcgccg aaatcgcgg tggcttgtc 
      tgacagtgg cgtacaagc cgaaggcgg aggaggaaa 
      ctcgaaaat tactcaaat caacgattg aaaattact 
      actcaccgc cggaaaata aagacgccg tgacgttga 
      tgtgggacc acctccagg ccaacttga aatgttgcg 
      gcgttaata attacaccg attatcgga gcagcacga 
      agtggacaa acagtggga aagtgattc ctgagtcgt 
      gctgcgtcc tcgaagggg atcccctga agttgaagc 
      cgaaggttc cgtcgtgtg tgacgagtc cgaacgaag 
      tcaactcgt actacaaac aaggttgct tcgatgcgc 
      tcgtccact ggatcatga tccacgttg atgtggtca 
      ttggtgtgg ctgtcggtt tgggtttgc tcgagttgg 
      tcgggatat ttctggcgt tttgtttag ctaaggccc 
      tcaatgggt acataaagt aggaggctt gggaccaag 
      cattatgtt aatctagtc gttacgtat agttttcgg 
      ggaaattca gtattattg taacagtgc ctttccgac 
      ttgcacgtg tacacacca atttggact tttctacat 
      gt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY