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Gene information
General information
TC011448 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 6193696..6198120
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011448
    iBeetle screen iB_05037

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011448 RA
      MDESKELEN TQEIDQFIE DQYLCELFG GGRCHLCAL 
      NRHNCLPSN MKDDSNEDI IQRSIGSLG RWHIFMCVI 
      ISFVKLPVA WLQLSIVFI APPASFTCT DNRTAQCGA 
      NCTGHNFDR SVFTETIIT EWDLVCNRA YLANLAQTL 
      TMLGILFGN MLFGYLSDR LGRRSPLIC AVFLQVLCG 
      MGAAVSPWF PLFLVLRFI AALATGGTM VMSFVLVME 
      IVGMKWRTT LGILYQIPF NMGLLTLPL FAYFLRDWH 
      HFHIAICVP GILLLSYYW LLPESPRWL LAVGRKSDA 
      IEVLQLAAR RNKRPTAPI PANVDTYME RRQLQGGAH 
      SGDILDLVR TPTMRTYTA CIGYNWFAC GLCFFGGAQ 
      YIGQLGGNI FVNIALSAV MQIPSTFFS LWAVSAWGR 
      KYTLVFSNV LSGLSCLLI GGIPPEPTW VRTVLSSVD 
      MFALAISFP TVYLYSGEL FPTVVRNAG VGTSSMFAR 
      VGSMAAPFV AGLVLVREW LPPLVFGLA PLIGALLCF 
      KLPETVGCK LPDTVEEAE ALGRKKVVE
    CDS sequence
    • >TC011448 RA
      atggacgag agcaaagag ttggaaaat acacaggaa 
      attgatcaa tttattgaa gatcagtat ctttgtgag 
      ctttttggt ggcggaaga tgccacttg tgcgccttg 
      aatagacac aattgcctt ccttccaac atgaaagac 
      gactccaac gaggacata atccagcgc agcatcggc 
      tccctcggc cgttggcac attttcatg tgtgttatc 
      atttccttc gtcaaactc ccagtagcc tggctccag 
      ctcagtatt gtttttatt gctcctcca gcctcattt 
      acttgcact gataaccga acggcgcaa tgtggcgct 
      aattgcacc ggacacaat ttcgatcgg tccgtattc 
      actgaaacc atcatcacc gaatgggac ctggtctgc 
      aaccgtgct tacctcgcc aacctggcc caaacttta 
      accatgcta ggcatcctc ttcggaaac atgcttttc 
      gggtactta tccgaccgc ctcggtcgc cgcagcccc 
      ctcatctgt gccgtcttt ctccaagtg ttgtgcggc 
      atgggggct gccgtctcc ccttggttc cccctcttc 
      ctcgttctg cgcttcatt gcagcccta gccactggg 
      ggcaccatg gtgatgagt tttgtccta gtcatggag 
      atagtcggc atgaaatgg cgcacaacg cttgggatc 
      ttgtaccaa atccccttc aatatggga ttattgacc 
      ttgccgttg ttcgcctac ttcctgcgg gattggcac 
      cacttccac atagccatc tgcgtcccc gggatcctc 
      ctgctgtcc tactactgg ctgctgccc gagtcccca 
      agatggctg ctcgcagtg gggcgcaaa agcgacgcc 
      atcgaagtg ctgcaattg gccgcgaga cgcaacaag 
      cgccccaca gcccccatc ccggccaac gtggacacc 
      tacatggag cggcgccag ctccaaggg ggcgcccac 
      agcggggac atcctggac ctggtccgc acccccacc 
      atgcgcacg tacaccgcc tgcatcggg tacaactgg 
      ttcgcctgc gggctgtgc ttcttcggg ggggcgcag 
      tacatcggc cagctgggg ggcaacatc ttcgtcaac 
      atcgcgttg tcggcagtt atgcaaatc ccaagcacg 
      tttttctcg ctgtgggcg gtgtcggca tgggggcgc 
      aagtacacg cttgtcttc tccaacgtc ctgtcgggg 
      ctgtcgtgt cttttgatc ggaggcatt ccgcctgag 
      cccacttgg gtcaggacg gtgctcagt tcggttgat 
      atgtttgca ttggcgatt tccttccca acggtctat 
      ttgtactcg ggggagctg ttccccact gttgtcagg 
      aatgcgggg gttgggacg agttcgatg tttgcaaga 
      gtgggctcg atggcggcg ccctttgtt gcggggctg 
      gtcttggtg agggagtgg ctgccgccg cttgttttc 
      gggctggca ccgctgatt ggggccctg ttgtgtttc 
      aagttgccg gagactgtg ggctgcaag ctgccggat 
      acggtggag gaggcggag gctttgggg aggaaaaag 
      gtggttgag tag
    mRNA sequence
    • >TC011448 RA
      tgagtttaa taaaacttg gcaattttg gatcttttg 
      tgttacaat ggacgagag caaagagtt ggaaaatac 
      acaggaaat tgatcaatt tattgaaga tcagtatct 
      ttgtgagct ttttggtgg cggaagatg ccacttgtg 
      cgccttgaa tagacacaa ttgccttcc ttccaacat 
      gaaagacga ctccaacga ggacataat ccagcgcag 
      catcggctc cctcggccg ttggcacat tttcatgtg 
      tgttatcat ttccttcgt caaactccc agtagcctg 
      gctccagct cagtattgt ttttattgc tcctccagc 
      ctcatttac ttgcactga taaccgaac ggcgcaatg 
      tggcgctaa ttgcaccgg acacaattt cgatcggtc 
      cgtattcac tgaaaccat catcaccga atgggacct 
      ggtctgcaa ccgtgctta cctcgccaa cctggccca 
      aactttaac catgctagg catcctctt cggaaacat 
      gcttttcgg gtacttatc cgaccgcct cggtcgccg 
      cagccccct catctgtgc cgtctttct ccaagtgtt 
      gtgcggcat gggggctgc cgtctcccc ttggttccc 
      cctcttcct cgttctgcg cttcattgc agccctagc 
      cactggggg caccatggt gatgagttt tgtcctagt 
      catggagat agtcggcat gaaatggcg cacaacgct 
      tgggatctt gtaccaaat ccccttcaa tatgggatt 
      attgacctt gccgttgtt cgcctactt cctgcggga 
      ttggcacca cttccacat agccatctg cgtccccgg 
      gatcctcct gctgtccta ctactggct gctgcccga 
      gtccccaag atggctgct cgcagtggg gcgcaaaag 
      cgacgccat cgaagtgct gcaattggc cgcgagacg 
      caacaagcg ccccacagc ccccatccc ggccaacgt 
      ggacaccta catggagcg gcgccagct ccaaggggg 
      cgcccacag cggggacat cctggacct ggtccgcac 
      ccccaccat gcgcacgta caccgcctg catcgggta 
      caactggtt cgcctgcgg gctgtgctt cttcggggg 
      ggcgcagta catcggcca gctgggggg caacatctt 
      cgtcaacat cgcgttgtc ggcagttat gcaaatccc 
      aagcacgtt tttctcgct gtgggcggt gtcggcatg 
      ggggcgcaa gtacacgct tgtcttctc caacgtcct 
      gtcggggct gtcgtgtct tttgatcgg aggcattcc 
      gcctgagcc cacttgggt caggacggt gctcagttc 
      ggttgatat gtttgcatt ggcgatttc cttcccaac 
      ggtctattt gtactcggg ggagctgtt ccccactgt 
      tgtcaggaa tgcgggggt tgggacgag ttcgatgtt 
      tgcaagagt gggctcgat ggcggcgcc ctttgttgc 
      ggggctggt cttggtgag ggagtggct gccgccgct 
      tgttttcgg gctggcacc gctgattgg ggccctgtt 
      gtgtttcaa gttgccgga gactgtggg ctgcaagct 
      gccggatac ggtggagga ggcggaggc tttggggag 
      gaaaaaggt ggttgagta ggaaagtgg aaataaagg 
      gttttcagt acatttttg gttttattt ttcagattt 
      aaattttca gtctttcat ttttttttg tttaaaaat 
      ataaatttt gacacaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY