Status
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Gene information
General information
TC011430 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 5104538..5125014
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011430
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05034

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011430 RA
      MVDLKGETV PITPKKSRG ISMTTVKNW LMPERKTQD 
      TPKFQRMPE SFLRSIRRR SLRVKRTKS LVLPEKRKS 
      DSFVNSQEW TLSRTVPDL RLGIVGSLS SGKSALVHR 
      YLTGSYMQE ESPEGGRFK KEILVDNQS YLLLIRDEG 
      GPPELQFTA WVDAVIFVF SLENESSFN AIYNYYTKM 
      SHYRNSAEI PLILVGTQD AISENNPRV IDDSRARKL 
      ANDLKRCSY YETCATYGL NVERVFQDD PDGYEFFIV 
      SLDNKQWHF EASSSDERD DWVAAIEQQ ILNSLQLNE 
      SSKGKKQNN PMEAATIQS IRSRVPGNG FCVDCDATN 
      PDWASLNLG VLMCIECSG IHRNLGSHI SRVRSLDLD 
      EWPAGHLSV MLAIGNTLA NSVWECRTQ GRTKPTPTS 
      SREEKERWI RAKYESKEF LPPPNTTMP LGQQLVDAV 
      CSSNMKAII HVLAMANTE QVNTTVAPR DLRTPLHLA 
      CAMGNLAVA QLLIWHNAN VKAVDQDGR TCLAYARAA 
      ASIAAAKSQ SNNSVSADV SAAASRSLV DLLLSYGCP 
      EVSCVPGSG TLPRRRGTT NQTMPQLPS SVI
    CDS sequence
    • >TC011430 RA
      atggtggat ttaaaaggt gaaacggtg ccaataaca 
      ccgaaaaaa tcgcgtgga atttcaatg acaacagtc 
      aaaaattgg ctaatgccc gaacggaaa actcaagac 
      acgccgaaa tttcaacga atgcctgaa agttttctc 
      cggtcgatc cggaggcgt tctttgagg gtgaaaaga 
      acgaagtcg ttggtctta ccggagaaa agaaagtcc 
      gattccttc gtgaatagc caagaatgg acgttatca 
      cgtaccgtt ccggacctg cggttgggc atcgtcggc 
      tccttatca tccggaaag tccgcccta gtccaccgc 
      tatttgacc ggatcttac atgcaggaa gagtcaccg 
      gaaggagga cgtttcaaa aaggaaata ctcgtcgac 
      aatcaaagc taccttttg ttgatacga gacgaaggc 
      ggacctccg gaactacag ttcaccgca tgggtggat 
      gctgtgatc ttcgttttt agtctagaa aacgaatct 
      tcatttaac gccatctac aactattac acgaagatg 
      tctcattat cggaactca gccgagatt ccactcatc 
      ttagttgga acgcaagat gcaataagc gagaataat 
      ccgcgagta atcgacgat tctcgagct aggaaactg 
      gctaacgac ctgaagagg tgttcttac tatgagact 
      tgtgccacc tacggactc aatgttgaa cgagtcttt 
      caagacgat ccggacggt tacgaattt tttatcgtg 
      tcattggac aacaaacaa tggcatttt gaagcgtca 
      agttcggac gaacgtgac gattgggtc gctgcaata 
      gaacaacag attttgaat tcactgcaa ttgaacgaa 
      tcatccaaa ggcaaaaag caaaacaat ccaatggaa 
      gctgcaaca atacaatca atacgtagt cgtgtacct 
      ggaaacggt ttttgtgtc gactgtgat gccactaat 
      ccggactgg gcaagcctg aacctcggc gtcctcatg 
      tgcatcgaa tgctcgggg attcaccgt aatctcgga 
      tctcatatt tctagagtt aggtcactt gatttggac 
      gaatggccg gccggacat ttgagcgta atgttggcc 
      atcggtaat acgttagcg aattcggtg tgggaatgt 
      cgaacacag ggccggact aaaccaacg cctacttcg 
      agtcgtgag gagaaggag aggtggatt agggcgaaa 
      tatgaaagt aaggagttt cttccgccg cctaacacc 
      acaatgcct ttagggcaa caactggtc gatgctgtc 
      tgcagttca aacatgaag gcaattatt catgtcttg 
      gcgatggct aacacggaa caagtcaat acaacggtg 
      gccccaagg gacctaagg acgcccctg catttggcc 
      tgcgcgatg ggcaatttg gccgttgcg cagttgctc 
      atttggcac aacgcgaac gtgaaggcg gtggaccaa 
      gacgggcgc acctgcctg gcgtacgcg cgcgccgcc 
      gccagcatc gccgccgcc aagagccag agcaacaac 
      agcgtgagc gccgacgtt tcggccgcc gccagccgc 
      agcctcgtc gaccttttg ctctcttac ggttgcccc 
      gaagtgtcc tgcgtgccc gggagcggg actttgccc 
      cgccgccgc ggcacaacc aatcaaacg atgcctcag 
      ctcccctcg agcgtgatc taa
    mRNA sequence
    • >TC011430 RA
      tgattccgg tattatccc gaaaatgcc gctccggtg 
      aataaaaag tgcacgtga tggtggatt taaaaggtg 
      aaacggtgc caataacac cgaaaaaat cgcgtggaa 
      tttcaatga caacagtca aaaattggc taatgcccg 
      aacggaaaa ctcaagaca cgccgaaat ttcaacgaa 
      tgcctgaaa gttttctcc ggtcgatcc ggaggcgtt 
      ctttgaggg tgaaaagaa cgaagtcgt tggtcttac 
      cggagaaaa gaaagtccg attccttcg tgaatagcc 
      aagaatgga cgttatcac gtaccgttc cggacctgc 
      ggttgggca tcgtcggct ccttatcat ccggaaagt 
      ccgccctag tccaccgct atttgaccg gatcttaca 
      tgcaggaag agtcaccgg aaggaggac gtttcaaaa 
      aggaaatac tcgtcgaca atcaaagct accttttgt 
      tgatacgag acgaaggcg gacctccgg aactacagt 
      tcaccgcat gggtggatg ctgtgatct tcgttttta 
      gtctagaaa acgaatctt catttaacg ccatctaca 
      actattaca cgaagatgt ctcattatc ggaactcag 
      ccgagattc cactcatct tagttggaa cgcaagatg 
      caataagcg agaataatc cgcgagtaa tcgacgatt 
      ctcgagcta ggaaactgg ctaacgacc tgaagaggt 
      gttcttact atgagactt gtgccacct acggactca 
      atgttgaac gagtctttc aagacgatc cggacggtt 
      acgaatttt ttatcgtgt cattggaca acaaacaat 
      ggcattttg aagcgtcaa gttcggacg aacgtgacg 
      attgggtcg ctgcaatag aacaacaga ttttgaatt 
      cactgcaat tgaacgaat catccaaag gcaaaaagc 
      aaaacaatc caatggaag ctgcaacaa tacaatcaa 
      tacgtagtc gtgtacctg gaaacggtt tttgtgtcg 
      actgtgatg ccactaatc cggactggg caagcctga 
      acctcggcg tcctcatgt gcatcgaat gctcgggga 
      ttcaccgta atctcggat ctcatattt ctagagtta 
      ggtcacttg atttggacg aatggccgg ccggacatt 
      tgagcgtaa tgttggcca tcggtaata cgttagcga 
      attcggtgt gggaatgtc gaacacagg gccggacta 
      aaccaacgc ctacttcga gtcgtgagg agaaggaga 
      ggtggatta gggcgaaat atgaaagta aggagtttc 
      ttccgccgc ctaacacca caatgcctt tagggcaac 
      aactggtcg atgctgtct gcagttcaa acatgaagg 
      caattattc atgtcttgg cgatggcta acacggaac 
      aagtcaata caacggtgg ccccaaggg acctaagga 
      cgcccctgc atttggcct gcgcgatgg gcaatttgg 
      ccgttgcgc agttgctca tttggcaca acgcgaacg 
      tgaaggcgg tggaccaag acgggcgca cctgcctgg 
      cgtacgcgc gcgccgccg ccagcatcg ccgccgcca 
      agagccaga gcaacaaca gcgtgagcg ccgacgttt 
      cggccgccg ccagccgca gcctcgtcg accttttgc 
      tctcttacg gttgccccg aagtgtcct gcgtgcccg 
      ggagcggga ctttgcccc gccgccgcg gcacaacca 
      atcaaacga tgcctcagc tcccctcga gcgtgatct 
      aaccaatga ctgaacgac ctgccccac cccaccact 
      cacacaggg tgcactcga aaagttttc gaaacctcg 
      tcgtgttca caccgattc gatgtctta ttgtagtac 
      aatcaagtt ttttattag cacagcgtg gaagtactt 
      acgactgac tggtcattt tttcaatat tgcactgtg 
      aagcgagta ttatgtaat aagcgacta tactattaa 
      aaaaatgaa atcttttat atgtgaatt tgtaatatt 
      tgtaccttg gacgtgatt gattgattg attgtttgt 
      tatttatct ggtttttct gtggactgt ggttacgat 
      tcgattact gattattta tgcaggaat actcccttt 
      gttgacgtt tttttcctt tgtatataa tttcgccaa 
      agatttgta ttgattgtt ttgtatata cggagctaa 
      attatgtac agaaattat atatgttgt tcttcctta 
      cataaaatg ttacgaaac tgagcacaa attttattt 
      acc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY