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Gene information
General information
TC011419 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 5409090..5412515
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011419
    iBeetle screen iB_05033

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011419 RA
      MASYEEWCE EKLSRLSDE DSRIPVLKE MKDFLTTIP 
      REEMSRTAN SLQLPAVFD CLNDSNSEQ VDLACEVLA 
      LCMTNLNLG ESTNKYGVF LERALNHPF SAVKLMALN 
      EIKRNIENE DILVDLCKR QSLLINIIK CMGDEDLGV 
      AKGAAGVIK ILGLSSVGI KQLTKADIL QSLHDIMGL 
      SEIVRFRVY ELLIDISNQ SEENFNILK GAGLFSQIF 
      NELDNNDIL LRLNVLELL TQLGSSKHG YNYLESNET 
      LKKLSAMLD SDDLVTVQL CEPGILKFF GNMAYWKPM 
      ETLAKYPAL FQRMFNNIE SNDFTIISV SVETLGHIG 
      LPNDGKIAL DSMGAAIEN AIKNISKSI PSMPSEVKV 
      HGLNCLENL LQIIEPNNQ VMAITHKWF SLIEDNPMS 
      FIWNYAKNP FFELRLGGL GLLRAVAGQ PWGQEAIKN 
      FPGMVEFLL DRSVETIKE CKEAKFGIV ELLANSEVF 
      DHTTLKKLQ KYVKEGPFY VQAVTEVAI ERND
    CDS sequence
    • >TC011419 RA
      atggcttcg tacgaagag tggtgtgaa gaaaaatta 
      tcaagactt tccgatgaa gattcgcga attccagta 
      ttaaaagaa atgaaagac ttcctgact acaatcccg 
      cgtgaggaa atgtcccga acagccaac tccctgcaa 
      ctccctgca gttttcgac tgtctaaac gactcaaac 
      agtgaacag gtcgattta gcttgcgaa gtcctcgca 
      ctttgtatg acgaattta aatttaggc gaaagcacg 
      aataaatac ggcgttttt ttagaaaga gctttaaat 
      catccattt tctgcggtt aagctcatg gctttaaat 
      gagattaaa cgtaatatt gaaaacgag gacattctt 
      gtcgacctg tgcaaaaga cagtcgctt ttaatcaat 
      attattaag tgcatgggg gacgaggat ctgggggtt 
      gctaagggg gcggcaggg gttatcaaa attttgggg 
      ctgtcgagc gtggggatc aagcagttg accaaggct 
      gatattttg cagtcgttg cacgatatt atgggactg 
      agcgaaatt gtcaggttt agagtttat gagttgttg 
      attgatatc agcaaccaa tccgaagag aatttcaac 
      attttgaag ggggctggt ctattttcc caaatcttt 
      aacgaactc gataataac gatattttg ctccgatta 
      aatgtcttg gagttgttg actcaatta ggctcaagt 
      aagcacggt tacaattat ttagaatcg aatgagact 
      ttaaaaaaa ttgagcgca atgttggac agtgatgat 
      ctagtaaca gttcagttg tgtgaacct ggaattttg 
      aaatttttt ggaaacatg gcctactgg aaaccaatg 
      gagaccctc gcaaaatat cccgctctg tttcaacga 
      atgttcaac aatatcgaa agcaacgat tttaccata 
      attagtgtc tctgttgag acacttgga cacattgga 
      ttgccaaac gacggtaaa attgcgttg gactcaatg 
      ggagctgct attgaaaat gcaattaaa aatatttca 
      aaatcaatc ccttcaatg cccagtgaa gtcaaagtg 
      catgggttg aattgtttg gaaaattta ttacaaatc 
      attgagccc aataaccaa gtgatggcg atcacacat 
      aaatggttt tctttgatt gaggataat cccatgagt 
      tttatttgg aattacgcg aaaaatccg tttttcgaa 
      ttgaggctt ggcgggctg ggtcttttg agggccgta 
      gcgggacag ccttggggc caggaggct attaaaaat 
      tttccagga atggttgaa tttctgctc gatcgaagc 
      gtggagacc ataaaagag tgtaaagaa gcaaagttt 
      gggattgtt gagttgttg gcaaatagt gaagttttc 
      gatcatacg actcttaaa aagttgcaa aaatacgtg 
      aaggagggg ccgttttat gtccaagcg gttacggaa 
      gttgcgatc gaaagaaat gattaa
    mRNA sequence
    • >TC011419 RA
      ttccaattt tcaaattgt cacgatggc ttcgtacga 
      agagtggtg tgaagaaaa attatcaag actttccga 
      tgaagattc gcgaattcc agtattaaa agaaatgaa 
      agacttcct gactacaat cccgcgtga ggaaatgtc 
      ccgaacagc caactccct gcaactccc tgcagtttt 
      cgactgtct aaacgactc aaacagtga acaggtcga 
      tttagcttg cgaagtcct cgcactttg tatgacgaa 
      tttaaattt aggcgaaag cacgaataa atacggcgt 
      ttttttaga aagagcttt aaatcatcc attttctgc 
      ggttaagct catggcttt aaatgagat taaacgtaa 
      tattgaaaa cgaggacat tcttgtcga cctgtgcaa 
      aagacagtc gcttttaat caatattat taagtgcat 
      gggggacga ggatctggg ggttgctaa gggggcggc 
      aggggttat caaaatttt ggggctgtc gagcgtggg 
      gatcaagca gttgaccaa ggctgatat tttgcagtc 
      gttgcacga tattatggg actgagcga aattgtcag 
      gtttagagt ttatgagtt gttgattga tatcagcaa 
      ccaatccga agagaattt caacatttt gaagggggc 
      tggtctatt ttcccaaat ctttaacga actcgataa 
      taacgatat tttgctccg attaaatgt cttggagtt 
      gttgactca attaggctc aagtaagca cggttacaa 
      ttatttaga atcgaatga gactttaaa aaaattgag 
      cgcaatgtt ggacagtga tgatctagt aacagttca 
      gttgtgtga acctggaat tttgaaatt ttttggaaa 
      catggccta ctggaaacc aatggagac cctcgcaaa 
      atatcccgc tctgtttca acgaatgtt caacaatat 
      cgaaagcaa cgattttac cataattag tgtctctgt 
      tgagacact tggacacat tggattgcc aaacgacgg 
      taaaattgc gttggactc aatgggagc tgctattga 
      aaatgcaat taaaaatat ttcaaaatc aatcccttc 
      aatgcccag tgaagtcaa agtgcatgg gttgaattg 
      tttggaaaa tttattaca aatcattga gcccaataa 
      ccaagtgat ggcgatcac acataaatg gttttcttt 
      gattgagga taatcccat gagttttat ttggaatta 
      cgcgaaaaa tccgttttt cgaattgag gcttggcgg 
      gctgggtct tttgagggc cgtagcggg acagccttg 
      gggccagga ggctattaa aaattttcc aggaatggt 
      tgaatttct gctcgatcg aagcgtgga gaccataaa 
      agagtgtaa agaagcaaa gtttgggat tgttgagtt 
      gttggcaaa tagtgaagt tttcgatca tacgactct 
      taaaaagtt gcaaaaata cgtgaagga ggggccgtt 
      ttatgtcca agcggttac ggaagttgc gatcgaaag 
      aaatgatta attttagtt gaatgttca tgtgttgat 
      ttcaaacaa taaaatgtt aatttatta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY