Status
Please wait ...
Gene information
General information
TC011404 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 5747235..5753988
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011404
    iBeetle screen iB_05031

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    vitellarium not present - Number of animals showing the phenotype: 4

    vitellogenic egg chamber not present - Number of animals showing the phenotype: 4
    previtellogenic egg chamber potentiallypresent - Number of animals showing the phenotype: 4

    Comment: potentially some previtellogenic eggs in posterior region of tropharium
    mature egg not present - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection



    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection



    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Pupal morphology
    eyes size decreased - Number of animals showing the phenotype: 2

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011404 RA
      MAVLQEFDR AISCVASKL DGSAPQPSD AGASIFTMS 
      TEDSSEDMD IAQQLKNME NVINLTRAN IDALNAKFA 
      DFQHPPPIY LMEYEDLTC KLHELELQE RRLIEQLSN 
      GRESPPELL EEPERRAAQ PHALALRPR FLRAHLPNQ 
      QRTSVQVRE GLTLRDALA KAMKLRNLI CEMCCVYLG 
      DTNTVVNWD TDISTLNCE EITVKILDK FPIPTSISH 
      NFVRKTYFS LVFCECCRR LLFQGFYCR TCGYKFHQR 
      CAGNVPPLC HQVRMTDAY YRALLARSP DFSAGILHP 
      GQAGLGLSR QPRHPGTLG HHDRSSSAP NVCLNNVKG 
      PGDDCPKSL SLTRGGIDD GLPHYHSTQ ASPTGSLQP 
      RRPRARSAD ESKPLLAPR ESIEDWEIP ADEILVGHR 
      VGSGSFGTV YKAHWHGPV AVKTLNVKI PTLAQLQAF 
      KNEVAVLRK TRHVNILLF MGCVSKPQL AIVTQWCES 
      SSLYKHLHV HETKFALFT LIEIGRQTA QGMDYLHAK 
      NIIHRDLKS NNIFLHDDL TVKIGDFGL ATAKIRWSG 
      SKQFHQPTG SILWMAPEV IRMQEDNPY SFQSDVYAF 
      GIVMFEMLA MQLPYSHIN NKDQILFMV GRGYLKPDM 
      SKLRSDTPK ALRRLTEDC IKFNRDERP LFRQIHASL 
      EGLLRSHPK INRSASEPN MNRTQIQSD DFIYPCASP 
      KTPVAFGPS SFFYSGGTG NI
    CDS sequence
    • >TC011404 RA
      atggcagtt ttgcaagag tttgatagg gcaatatct 
      tgcgttgcg tctaagttg gatgggagt gcccctcag 
      ccgtctgat gccggtgcg agcatcttc acaatgtcg 
      accgaagac agcagcgaa gacatggac atcgcgcag 
      caactcaag aacatggag aacgttatt aacctgacc 
      cgcgccaac atcgacgcc cttaacgcc aagttcgcc 
      gacttccag cacccgccg cccatctac ctgatggag 
      tacgaggac ctcacctgc aagctgcac gaactagag 
      ctccaggag cgccgcctc atcgagcag ctgagcaac 
      ggccgcgag agccccccc gagctgctg gaggagccc 
      gagcggcgc gccgcacag ccccacgcc ctcgccctc 
      aggcctagg ttcctcagg gcgcatctg cccaaccag 
      cagcgcacc agcgtccaa gtacgcgag gggctcacg 
      ctgcgagac gccttagcc aaagcaatg aaactacgt 
      aaccttatt tgtgaaatg tgctgtgtt tatctcggg 
      gatactaac actgtggtg aattgggac acggatatt 
      agcaccctc aactgcgag gagatcacc gttaaaata 
      ctcgacaag tttcccatt ccaacgagt atttcgcat 
      aatttcgtt agaaagact tacttctcg ctggtgttc 
      tgtgaatgc tgtcggaga cttttattt caagggttt 
      tattgtagg acttgcgga tacaagttc caccaaagg 
      tgtgccgga aacgtacct ccattgtgt caccaagtt 
      cgtatgacg gacgcctac taccgggcg ctcctcgcc 
      cgctctccg gacttcagc gcgggcatc ctgcaccca 
      ggtcaggcc ggcctgggg ctctcccgc cagccgcgc 
      caccctggc accctcggc caccacgac cgctccagc 
      tccgccccc aacgtctgc ctcaacaac gtgaagggc 
      cccggcgac gactgcccc aagtccctc agtctgacc 
      cggggcggc atcgacgac ggcctcccc cactaccac 
      agcacccag gcctcgccc accggctcc ctccagccg 
      cgccgaccc cgtgctcgc tccgccgac gagagcaag 
      cccctcctc gcccctcgc gagagcatc gaggattgg 
      gagatcccc gccgacgag atcctcgtc ggccatcgg 
      gtcggctcg ggctccttc ggcacggtc tacaaggcc 
      cattggcac ggcccggtc gctgtcaag acgctcaac 
      gttaaaata ccaacgttg gcacaactg caagccttc 
      aagaacgag gtggcagta ctgcgcaaa acgcgacac 
      gtgaacatt ttattgttc atggggtgt gttagtaag 
      ccgcagttg gcgatcgtg acgcaatgg tgcgagagt 
      tcgagcttg tacaagcat ctccacgtg cacgagacg 
      aaattcgcg ctttttact ttgatcgaa atcgggaga 
      caaacggca caaggaatg gactacctc catgcgaaa 
      aatatcatc catcgagat ctcaaatcg aataatatt 
      ttcctacac gatgaccta accgtcaaa atcggcgat 
      ttcggctta gccacggcc aagatccgt tggtcgggc 
      tccaagcag ttccaccag ccgacgggg agcatcctc 
      tggatggcg ccagaagtg atccggatg caggaggac 
      aacccgtac agcttccag tcggacgtt tacgcgttc 
      gggatcgtt atgttcgag atgttggca atgcaattg 
      ccctactcc cacatcaac aacaaggac caaatcctg 
      ttcatggtg gggaggggg tacctcaaa ccagacatg 
      agtaaacta cgctccgac acacccaaa gctttaaga 
      cgactcact gaagactgt atcaagttc aatcgggac 
      gagagaccg cttttcagg cagattcat gcctcgttg 
      gagggactt ttgaggagt catccgaaa attaacaga 
      tcggcgtcc gaaccgaac atgaatcgg acgcagatc 
      cagtctgat gattttatt tatccgtgt gctagtccg 
      aaaacaccg gtggcgttc ggaccgtcg agttttttt 
      tattcggga ggaacgggt aatatctga
    mRNA sequence
    • >TC011404 RA
      gggcgctgc agtgtcgtg aaagtccct agtcagttt 
      gtccaacct ccgaaatgt catttgtca ggtaaaatg 
      ggcaagtgc aatggcagt tttgcaaga gtttgatag 
      ggcaatatc ttgcgttgc gtctaagtt ggatgggag 
      tgcccctca gccgtctga tgccggtgc gagcatctt 
      cacaatgtc gaccgaaga cagcagcga agacatgga 
      catcgcgca gcaactcaa gaacatgga gaacgttat 
      taacctgac ccgcgccaa catcgacgc ccttaacgc 
      caagttcgc cgacttcca gcacccgcc gcccatcta 
      cctgatgga gtacgagga cctcacctg caagctgca 
      cgaactaga gctccagga gcgccgcct catcgagca 
      gctgagcaa cggccgcga gagcccccc cgagctgct 
      ggaggagcc cgagcggcg cgccgcaca gccccacgc 
      cctcgccct caggcctag gttcctcag ggcgcatct 
      gcccaacca gcagcgcac cagcgtcca agtacgcga 
      ggggctcac gctgcgaga cgccttagc caaagcaat 
      gaaactacg taaccttat ttgtgaaat gtgctgtgt 
      ttatctcgg ggatactaa cactgtggt gaattggga 
      cacggatat tagcaccct caactgcga ggagatcac 
      cgttaaaat actcgacaa gtttcccat tccaacgag 
      tatttcgca taatttcgt tagaaagac ttacttctc 
      gctggtgtt ctgtgaatg ctgtcggag acttttatt 
      tcaagggtt ttattgtag gacttgcgg atacaagtt 
      ccaccaaag gtgtgccgg aaacgtacc tccattgtg 
      tcaccaagt tcgtatgac ggacgccta ctaccgggc 
      gctcctcgc ccgctctcc ggacttcag cgcgggcat 
      cctgcaccc aggtcaggc cggcctggg gctctcccg 
      ccagccgcg ccaccctgg caccctcgg ccaccacga 
      ccgctccag ctccgcccc caacgtctg cctcaacaa 
      cgtgaaggg ccccggcga cgactgccc caagtccct 
      cagtctgac ccggggcgg catcgacga cggcctccc 
      ccactacca cagcaccca ggcctcgcc caccggctc 
      cctccagcc gcgccgacc ccgtgctcg ctccgccga 
      cgagagcaa gcccctcct cgcccctcg cgagagcat 
      cgaggattg ggagatccc cgccgacga gatcctcgt 
      cggccatcg ggtcggctc gggctcctt cggcacggt 
      ctacaaggc ccattggca cggcccggt cgctgtcaa 
      gacgctcaa cgttaaaat accaacgtt ggcacaact 
      gcaagcctt caagaacga ggtggcagt actgcgcaa 
      aacgcgaca cgtgaacat tttattgtt catggggtg 
      tgttagtaa gccgcagtt ggcgatcgt gacgcaatg 
      gtgcgagag ttcgagctt gtacaagca tctccacgt 
      gcacgagac gaaattcgc gctttttac tttgatcga 
      aatcgggag acaaacggc acaaggaat ggactacct 
      ccatgcgaa aaatatcat ccatcgaga tctcaaatc 
      gaataatat tttcctaca cgatgacct aaccgtcaa 
      aatcggcga tttcggctt agccacggc caagatccg 
      ttggtcggg ctccaagca gttccacca gccgacggg 
      gagcatcct ctggatggc gccagaagt gatccggat 
      gcaggagga caacccgta cagcttcca gtcggacgt 
      ttacgcgtt cgggatcgt tatgttcga gatgttggc 
      aatgcaatt gccctactc ccacatcaa caacaagga 
      ccaaatcct gttcatggt ggggagggg gtacctcaa 
      accagacat gagtaaact acgctccga cacacccaa 
      agctttaag acgactcac tgaagactg tatcaagtt 
      caatcggga cgagagacc gcttttcag gcagattca 
      tgcctcgtt ggagggact tttgaggag tcatccgaa 
      aattaacag atcggcgtc cgaaccgaa catgaatcg 
      gacgcagat ccagtctga tgattttat ttatccgtg 
      tgctagtcc gaaaacacc ggtggcgtt cggaccgtc 
      gagtttttt ttattcggg aggaacggg taatatctg 
      aggggaggg cgccaagag ggacgttta ggacgagtg 
      agagtgcgt gatacggct tgagtagag gttatcatt 
      aagttattg tcaagtatt taaatttaa atattgttt 
      tgttttcgt attaaataa attcttaaa aaaata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY