Status
Please wait ...
Gene information
General information
TC031400
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011402,TC011403
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 5765542..5774760
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031400
    iBeetle screen iB_05030

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031400 RA
      MKVTDNETR WAKIGEIKD LSFIGKEVL FYGTGCHLI 
      FINLSTGEE LLYTANNAK VGDGIQCYN GHRTSPVFA 
      FAEKKTNPT LFIKSYPNF DEIHQFKDG APRGYISMS 
      FSETSLLIT LGDLPDFNI TVWNWRRHE KIASQNGTI 
      LMQDQILRC NFTSPVCLA QLGLYSHKL HVWDIFVCG 
      KKCILTQHV ARIEEDCAP FCGVLWTTE GGLFVLDAL 
      GAIYTMFPD HHLEKVIDP PESGPYPTS FTWYKFGIA 
      VAGPNLEIR HYKKSGTWT MDWSLVPPQ PIQKIQSRF 
      DYLIAITTK TDLIQVLPT QKDFKYVKI NESSFDDFC 
      MVYPTGEYL LALTHGHVV NSYKVATGE MVGTIELDG 
      TATLIVENP KFPYAAVGF ENGILNLVS AYNPQKLTK 
      MTQFFLTKH PPNSIRFNE CGTVFVVGH LTIGRFFVI 
      EGLPGGQME VIAFIDAQR QIADYTIVI SQDCYRIFM 
      LPVTSDQFM AGNMILRYC YIKNKGTDV KEYYFEEKN 
      TLFARIVAT VGANRDRMF YLIPLEGKT IAEVEIKKS 
      EILAKITKK IVTGHQMQN FHFRLDRKV ALTWGYDGF 
      VIARGADFE TNIGIGLPH HRMQGGVKK AFLGPYQKY 
      MLTLGRDRV LACTNLMER HIDSKLKQE YLDVLHSDD 
      YKLLFTRRT LGYFPRGIY EGKCYLEVQ EMKKLEKEY 
      LQCKKERDS ILAEFRSIQ KELQDLLTA NLEGPDNEK 
      LDMKEFNLN TTLYNQKAE FQKKLRKAT ELYLKNLIQ 
      AQNVISNYM MTNYWDKMK VNGAAIKAI FANFEVTNY 
      VLRPPDLNR EELLNWIRH QRRIERYLS EMNSFHPWE 
      PIPEDKLLE LVSTRPSLP VKDEHLAVL SALEIDAPE 
      MEVEEEDIE SKIAFSGSD TARFIEVQD AHYLQNELQ 
      TYFQTELQW IISKSDSYK LKEFFNEKY EKVMLQKMK 
      EMSSIKEKN ERLRHIVSE INYFSEEKI YVTIDDPHW 
      EQVETPELL TQVKDSEVP ITPYISPSE QAILDAKAA 
      EAERLRLLL LADDFRERA LMAMMNGVL EVRWEDELR 
      KEVPLPKCM IEKEPENFN EDDLRAVRD YEDKVQFLN 
      SERERYKTL LRAEYAKLS KSVRENIKK FNQKLSDLL 
      HLRLQVDAA ISQETLKIS RTKLFQHKR KLLFQKEQA 
      ILQKIANND EVVASLHKL MTTLHEAVT ECRNFIESY 
      QVKERYLER AFKKDFQDL SIVVQEQAT KLFKKRPKI 
      NHRLISSAT ILNELGKCI ISGAKPMTL TQEALDFLK 
      QLDNLDLYV GVPTVVDEH TYAVICKHR RLRIEYEVK 
      LKAAQNELA EAEATAAAF HKRLHNKKE FGAKLHNDL 
      NDVRQEFMT MMCNGDVQI VLPRGLVEI PLTGSMVDF 
      EDAVLICRK DVEEINRII LEFGDKKIK ALERGMNFR 
      RKILTQEWE HKRRRMLIQ DLQEKIIDI DGVRLTKEI 
      QFYLKSKDK EKAIPFDTE VDMIKASYE NTIQDRKDK 
      VNRMQKQIN AIKAHDKVV ERKITELNV DVCEFNLVK 
      DDELDARER ALIDARMKT LLTRSKMVQ QIQGNHHEI 
      LMLQTELEL LRLRTFPTL KYKLC
    CDS sequence
    • >TC031400 RA
      atgaaagtg accgataac gaaactcgt tgggcaaaa 
      atcggcgaa atcaaagac ctgtcattc atcggcaaa 
      gaagtgctt ttttatggc acaggctgt catctcatt 
      tttatcaat ttgagcacc ggtgaggag cttctttac 
      accgcaaac aatgcgaaa gtcggagat ggaatccag 
      tgttacaac ggccacaga accagccct gtattcgca 
      ttcgcggag aaaaaaacc aatccgacc cttttcatc 
      aaaagttac ccaaacttt gacgaaatc caccaattc 
      aaagacggc gcccctcgc gggtacatt tccatgtcc 
      ttttccgaa acttccctt ctgataaca ctgggggat 
      ttacccgat tttaacata accgtctgg aattggcgc 
      cgacatgag aaaatcgcc agtcaaaac ggcacaatc 
      ttaatgcag gaccaaatc ttgagatgc aactttact 
      agtccggtt tgtttggcc caattgggc ctctacagt 
      cacaaactc cacgtttgg gacattttt gtctgtggg 
      aaaaagtgc attttgacg caacacgtt gcccggatt 
      gaggaagat tgcgcccct ttttgcggg gttttgtgg 
      acgaccgaa ggcgggctc tttgtatta gacgccttg 
      ggggctatc tacacgatg ttccctgac caccacttg 
      gaaaaagtg atcgacccc cctgagtct ggcccttac 
      cccacctcc ttcacgtgg tacaaattt gggattgct 
      gtcgcaggc cccaacctt gaaattcgg cattacaaa 
      aaatccggt acttggacc atggactgg tccctggta 
      ccaccacaa ccaatccag aaaatccaa tccagattt 
      gactatttg attgcaata acgaccaaa actgatctg 
      attcaagtc ctaccaaca cagaaagat ttcaaatac 
      gttaaaata aacgaatcg agttttgat gatttttgt 
      atggtttac cccactgga gagtacctg ctggccttg 
      actcacggt cacgtggtt aattcctac aaggtggcc 
      actggagaa atggtgggc acaatcgaa ctcgatggt 
      accgcaact ttgattgtt gaaaatccc aaatttcct 
      tacgcagct gttggtttt gaaaatggg attcttaat 
      ctagtttcg gcctataat ccccaaaag ctgaccaaa 
      atgacgcaa ttttttcta actaaacac cctccaaac 
      agtattcgg ttcaacgaa tgtggtact gttttcgta 
      gttggccat ttgacaatc gggcgtttt ttcgtaatt 
      gagggactc cccggagga caaatggag gtcattgcc 
      ttcatagac gctcaaaga caaattgcc gactacaca 
      atcgtcata tcgcaagac tgctaccgt attttcatg 
      cttccggta acctccgat cagttcatg gccggaaac 
      atgatcctc cggtactgc tacatcaaa aacaaggga 
      acagacgta aaagagtac tactttgaa gagaagaac 
      acacttttt gcacgaatt gttgctact gttggagcc 
      aacagggac cgaatgttt tatctaatc ccacttgaa 
      ggcaaaaca atcgcagag gttgagatt aaaaagagc 
      gaaattttg gcaaaaatc accaaaaaa atcgtgacg 
      ggacaccaa atgcaaaac tttcatttc cggcttgat 
      agaaaagtt gccttgacc tgggggtac gatgggttt 
      gtgatagca cgaggagca gattttgag actaacatc 
      ggcattggg ttaccccat cacaggatg caaggtgga 
      gtcaaaaaa gccttttta gggccgtac caaaaatac 
      atgctcaca cttggaaga gacagagtt ctagcttgc 
      acaaatcta atggagaga cacattgat tccaaattg 
      aagcaagaa tatctcgac gttttacat tcagatgat 
      tacaaactt ctcttcacc cgaagaacc ttggggtac 
      ttcccccga ggcatctac gaaggaaag tgctacttg 
      gaggttcaa gaaatgaaa aaactggag aaggagtac 
      ctccagtgt aagaaagaa cgagactcg attttggcc 
      gaatttcga tcgatacaa aaggaactc caagacttg 
      ctaacagcg aatctcgaa ggtcccgat aacgaaaaa 
      cttgacatg aaagaattc aatctcaat acaactctg 
      tataaccaa aaggccgag tttcagaaa aaattgagg 
      aaagcaact gaactctac ttgaaaaat cttatacag 
      gcccaaaac gttatttcc aattacatg atgacaaac 
      tactgggac aaaatgaaa gtcaacggg gccgcaatt 
      aaggcaatt tttgccaat tttgaggtc acgaattac 
      gttttgcga ccaccagat cttaatcgt gaggaactt 
      cttaactgg attaggcac cagagacga atcgagcga 
      tatttgagc gagatgaac tcgtttcat ccctgggaa 
      cccatacct gaagacaaa ttattagag ttggtgtca 
      actcgaccg tcacttccg gtcaaagac gagcatttg 
      gccgtgtta tcggcactt gaaatagac gctcctgaa 
      atggaagtt gaagaagag gacattgag tcaaaaatt 
      gctttttct ggctcagac acggcaaga tttatcgaa 
      gtccaagac gctcactac ttgcaaaac gagctccag 
      acctacttc caaaccgaa ttacaatgg attatttct 
      aagagcgat tcctacaag ttgaaagag ttcttcaat 
      gagaaatac gagaaggta atgttacaa aaaatgaaa 
      gaaatgtca agtatcaag gagaagaac gagcgtttg 
      cgccacata gtctcagaa atcaattac ttttccgaa 
      gagaagatt tatgtgacc attgatgat cctcattgg 
      gagcaagtt gaaactcct gaacttctg acccaagtt 
      aaagatagc gaagttccc ataactccc tacatctca 
      ccaagtgag caagcaatt ctcgatgct aaagcagca 
      gaagcggaa aggttacgt ctgctactc cttgctgat 
      gatttccgc gaaagagcc ctaatggct atgatgaac 
      ggagttttg gaagttcga tgggaggac gaactgcgt 
      aaagaagtg cctctcccc aaatgcatg atcgaaaaa 
      gaaccagaa aacttcaat gaggatgat ctaagagct 
      gtgcgagac tacgaagac aaggtccag tttctgaac 
      agcgaaagg gaacgctac aagaccctt ttaagggcc 
      gagtatgcc aaactgtcc aagtctgtt cgcgaaaat 
      attaagaaa ttcaaccaa aaactgagc gatttgctc 
      cacttgcga ctgcaagtt gatgcggcc atcagtcag 
      gagactttg aaaattagc aggacgaaa ctgttccaa 
      cacaagaga aaactgctg tttcaaaaa gaacaagca 
      attttgcaa aaaattgca aacaatgac gaagtggtg 
      gcaagtctg cacaaactc atgaccact ttacacgag 
      gcggtgacc gagtgtcgc aactttatc gagtcttat 
      caagtcaag gagaggtac cttgagcgt gccttcaag 
      aaagacttc caggatttg tcgattgtg gtccaagaa 
      caagccaca aaattgttc aagaaacgt ccaaaaatc 
      aatcaccgg ctgatcagt tcggctaca attctcaac 
      gagttgggc aaatgcatt atttcgggt gccaaaccg 
      atgacgctg actcaagaa gctttagat tttttgaaa 
      cagttggat aacttggat ttgtatgtt ggagtaccg 
      acggttgtt gatgagcac acttatgct gtgatctgc 
      aagcatcgg aggttacgg atcgagtac gaagtcaag 
      ctgaaggct gctcagaac gagttggct gaagctgaa 
      gccaccgca gctgccttc cacaagcgg ttacacaac 
      aaaaaagag tttggagcc aaactgcac aacgatttg 
      aacgacgtt aggcaagaa ttcatgacg atgatgtgc 
      aacggtgat gtgcaaatt gtcctaccg agagggtta 
      gttgagata ccactaacc ggaagtatg gtggatttt 
      gaagacgcg gtcctgatt tgtcggaaa gatgttgaa 
      gaaatcaac agaatcatc ttggaattt ggtgataaa 
      aagatcaaa gctctagaa cgtggtatg aacttccgg 
      cgaaaaatt ttgacacag gagtgggaa cacaaaaga 
      cgaagaatg ttgattcag gacctccaa gagaagatt 
      attgacatt gacggtgtt cggctgact aaagaaatc 
      cagttctac ttgaaaagc aaagataag gagaaggcg 
      attccgttc gacaccgaa gttgacatg atcaaagcc 
      tcgtacgag aacacgatt caagacagg aaagacaaa 
      gtcaatcgc atgcaaaag caaatcaac gcgattaaa 
      gcacacgat aaggtggtt gagcgaaaa atcacagaa 
      ttgaacgtc gatgtgtgc gaatttaat ttggtcaaa 
      gatgatgag cttgatgcc agagaacga gccttgatc 
      gatgctagg atgaaaacg ttgctaacg aggtcgaag 
      atggtgcaa cagattcag ggcaaccac cacgaaatt 
      ctgatgctg caaacggag ttggaattg ttgagacta 
      aggactttt ccgacactc aaatacaag ctgtgttag
    mRNA sequence
    • >TC031400 RA
      caacacaga agtgacaga attgcgatg aaagtgacc 
      gataacgaa actcgttgg gcaaaaatc ggcgaaatc 
      aaagacctg tcattcatc ggcaaagaa gtgcttttt 
      tatggcaca ggctgtcat ctcattttt atcaatttg 
      agcaccggt gaggagctt ctttacacc gcaaacaat 
      gcgaaagtc ggagatgga atccagtgt tacaacggc 
      cacagaacc agccctgta ttcgcattc gcggagaaa 
      aaaaccaat ccgaccctt ttcatcaaa agttaccca 
      aactttgac gaaatccac caattcaaa gacggcgcc 
      cctcgcggg tacatttcc atgtccttt tccgaaact 
      tcccttctg ataacactg ggggattta cccgatttt 
      aacataacc gtctggaat tggcgccga catgagaaa 
      atcgccagt caaaacggc acaatctta atgcaggac 
      caaatcttg agatgcaac tttactagt ccggtttgt 
      ttggcccaa ttgggcctc tacagtcac aaactccac 
      gtttgggac atttttgtc tgtgggaaa aagtgcatt 
      ttgacgcaa cacgttgcc cggattgag gaagattgc 
      gcccctttt tgcggggtt ttgtggacg accgaaggc 
      gggctcttt gtattagac gccttgggg gctatctac 
      acgatgttc cctgaccac cacttggaa aaagtgatc 
      gacccccct gagtctggc ccttacccc acctccttc 
      acgtggtac aaatttggg attgctgtc gcaggcccc 
      aaccttgaa attcggcat tacaaaaaa tccggtact 
      tggaccatg gactggtcc ctggtacca ccacaacca 
      atccagaaa atccaatcc agatttgac tatttgatt 
      gcaataacg accaaaact gatctgatt caagtccta 
      ccaacacag aaagatttc aaatacgtt aaaataaac 
      gaatcgagt tttgatgat ttttgtatg gtttacccc 
      actggagag tacctgctg gccttgact cacggtcac 
      gtggttaat tcctacaag gtggccact ggagaaatg 
      gtgggcaca atcgaactc gatggtacc gcaactttg 
      attgttgaa aatcccaaa tttccttac gcagctgtt 
      ggttttgaa aatgggatt cttaatcta gtttcggcc 
      tataatccc caaaagctg accaaaatg acgcaattt 
      tttctaact aaacaccct ccaaacagt attcggttc 
      aacgaatgt ggtactgtt ttcgtagtt ggccatttg 
      acaatcggg cgttttttc gtaattgag ggactcccc 
      ggaggacaa atggaggtc attgccttc atagacgct 
      caaagacaa attgccgac tacacaatc gtcatatcg 
      caagactgc taccgtatt ttcatgctt ccggtaacc 
      tccgatcag ttcatggcc ggaaacatg atcctccgg 
      tactgctac atcaaaaac aagggaaca gacgtaaaa 
      gagtactac tttgaagag aagaacaca ctttttgca 
      cgaattgtt gctactgtt ggagccaac agggaccga 
      atgttttat ctaatccca cttgaaggc aaaacaatc 
      gcagaggtt gagattaaa aagagcgaa attttggca 
      aaaatcacc aaaaaaatc gtgacggga caccaaatg 
      caaaacttt catttccgg cttgataga aaagttgcc 
      ttgacctgg gggtacgat gggtttgtg atagcacga 
      ggagcagat tttgagact aacatcggc attgggtta 
      ccccatcac aggatgcaa ggtggagtc aaaaaagcc 
      tttttaggg ccgtaccaa aaatacatg ctcacactt 
      ggaagagac agagttcta gcttgcaca aatctaatg 
      gagagacac attgattcc aaattgaag caagaatat 
      ctcgacgtt ttacattca gatgattac aaacttctc 
      ttcacccga agaaccttg gggtacttc ccccgaggc 
      atctacgaa ggaaagtgc tacttggag gttcaagaa 
      atgaaaaaa ctggagaag gagtacctc cagtgtaag 
      aaagaacga gactcgatt ttggccgaa tttcgatcg 
      atacaaaag gaactccaa gacttgcta acagcgaat 
      ctcgaaggt cccgataac gaaaaactt gacatgaaa 
      gaattcaat ctcaataca actctgtat aaccaaaag 
      gccgagttt cagaaaaaa ttgaggaaa gcaactgaa 
      ctctacttg aaaaatctt atacaggcc caaaacgtt 
      atttccaat tacatgatg acaaactac tgggacaaa 
      atgaaagtc aacggggcc gcaattaag gcaattttt 
      gccaatttt gaggtcacg aattacgtt ttgcgacca 
      ccagatctt aatcgtgag gaacttctt aactggatt 
      aggcaccag agacgaatc gagcgatat ttgagcgag 
      atgaactcg tttcatccc tgggaaccc atacctgaa 
      gacaaatta ttagagttg gtgtcaact cgaccgtca 
      cttccggtc aaagacgag catttggcc gtgttatcg 
      gcacttgaa atagacgct cctgaaatg gaagttgaa 
      gaagaggac attgagtca aaaattgct ttttctggc 
      tcagacacg gcaagattt atcgaagtc caagacgct 
      cactacttg caaaacgag ctccagacc tacttccaa 
      accgaatta caatggatt atttctaag agcgattcc 
      tacaagttg aaagagttc ttcaatgag aaatacgag 
      aaggtaatg ttacaaaaa atgaaagaa atgtcaagt 
      atcaaggag aagaacgag cgtttgcgc cacatagtc 
      tcagaaatc aattacttt tccgaagag aagatttat 
      gtgaccatt gatgatcct cattgggag caagttgaa 
      actcctgaa cttctgacc caagttaaa gatagcgaa 
      gttcccata actccctac atctcacca agtgagcaa 
      gcaattctc gatgctaaa gcagcagaa gcggaaagg 
      ttacgtctg ctactcctt gctgatgat ttccgcgaa 
      agagcccta atggctatg atgaacgga gttttggaa 
      gttcgatgg gaggacgaa ctgcgtaaa gaagtgcct 
      ctccccaaa tgcatgatc gaaaaagaa ccagaaaac 
      ttcaatgag gatgatcta agagctgtg cgagactac 
      gaagacaag gtccagttt ctgaacagc gaaagggaa 
      cgctacaag accctttta agggccgag tatgccaaa 
      ctgtccaag tctgttcgc gaaaatatt aagaaattc 
      aaccaaaaa ctgagcgat ttgctccac ttgcgactg 
      caagttgat gcggccatc agtcaggag actttgaaa 
      attagcagg acgaaactg ttccaacac aagagaaaa 
      ctgctgttt caaaaagaa caagcaatt ttgcaaaaa 
      attgcaaac aatgacgaa gtggtggca agtctgcac 
      aaactcatg accacttta cacgaggcg gtgaccgag 
      tgtcgcaac tttatcgag tcttatcaa gtcaaggag 
      aggtacctt gagcgtgcc ttcaagaaa gacttccag 
      gatttgtcg attgtggtc caagaacaa gccacaaaa 
      ttgttcaag aaacgtcca aaaatcaat caccggctg 
      atcagttcg gctacaatt ctcaacgag ttgggcaaa 
      tgcattatt tcgggtgcc aaaccgatg acgctgact 
      caagaagct ttagatttt ttgaaacag ttggataac 
      ttggatttg tatgttgga gtaccgacg gttgttgat 
      gagcacact tatgctgtg atctgcaag catcggagg 
      ttacggatc gagtacgaa gtcaagctg aaggctgct 
      cagaacgag ttggctgaa gctgaagcc accgcagct 
      gccttccac aagcggtta cacaacaaa aaagagttt 
      ggagccaaa ctgcacaac gatttgaac gacgttagg 
      caagaattc atgacgatg atgtgcaac ggtgatgtg 
      caaattgtc ctaccgaga gggttagtt gagatacca 
      ctaaccgga agtatggtg gattttgaa gacgcggtc 
      ctgatttgt cggaaagat gttgaagaa atcaacaga 
      atcatcttg gaatttggt gataaaaag atcaaagct 
      ctagaacgt ggtatgaac ttccggcga aaaattttg 
      acacaggag tgggaacac aaaagacga agaatgttg 
      attcaggac ctccaagag aagattatt gacattgac 
      ggtgttcgg ctgactaaa gaaatccag ttctacttg 
      aaaagcaaa gataaggag aaggcgatt ccgttcgac 
      accgaagtt gacatgatc aaagcctcg tacgagaac 
      acgattcaa gacaggaaa gacaaagtc aatcgcatg 
      caaaagcaa atcaacgcg attaaagca cacgataag 
      gtggttgag cgaaaaatc acagaattg aacgtcgat 
      gtgtgcgaa tttaatttg gtcaaagat gatgagctt 
      gatgccaga gaacgagcc ttgatcgat gctaggatg 
      aaaacgttg ctaacgagg tcgaagatg gtgcaacag 
      attcagggc aaccaccac gaaattctg atgctgcaa 
      acggagttg gaattgttg agactaagg acttttccg 
      acactcaaa tacaagctg tgttaggtt tatgttgta 
      tttcattat taataaatt cgttttttg aat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY