Status
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Gene information
General information
TC031399
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011401,TC011400
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 5777433..5794004
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031399
    iBeetle screen iB_05029

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031399 RA
      MLLLVITIS LYFHKFSQA ETLKIGAIF DTDDPIKER 
      AFHHAIHQI EPIHGRTIE GLVKNVPPN DPFEAMLAA 
      CHLIESGAV AILGPTTHE NAHMVQTVC DNKDIPLLD 
      VRSVAHPQN SINFYPLQQ ILTQIYIKL LEAWNFENF 
      VILYENDDS LIRLAELLK FYGNGHRMV VRQLDKYQN 
      GNYRPTLKE VWRSGATHF VLDCSTDIL EEVLHQAQQ 
      VGLVTNKQF YIITNLDFH TLDLTSFQY SETNITGMR 
      FIDPDSDEI QNLGLTLYR NDFTNTEFG FIEAWKVNL 
      EMALIIDAV TMFGEVLNR LPKDFAIPS IDCASDKAW 
      TYGTTLTNL VKSVKYPGY TGLIQFDNF GLRSAFGLE 
      IIELKEGGI IKIGNWNYS DGLNINRVY PPDPPPLVE 
      GSLVNRTFI VITCLTEPY GMRRDSEVP LYGNERYEG 
      FGIDLIAEL SKKLGFNYT FIIREDKKN GEFDESSGE 
      WTGMIGDVI SGKADLAIT DLTITSERE SAVDFSTTF 
      MSLGISILY QKPKKALPS FFSFADPFS LTVWKLLAA 
      AFFGASIAL FILGRISPS EWQNPYPCV EDEFLVNQL 
      SLRNCVWFM VGSLMQQGS EIAPIAFST RMVAGMWWF 
      FTLIMVSSY TANLAAFLT TESPDLPFK DVFELVQVA 
      EKKGIKFGA KINGSTEKF FLDSKHVDE YQQIYKYMK 
      NHEDEVMVN DNKDGVHKA EHEDYAFFM ETTSIEYET 
      QRRCGLTSV GHSLDEKGY GIAMRKNSS YRMALSTAI 
      LKLQEEGVL AKLKRKWWE EQRGGGLCP QGEKSTEGT 
      PLNLKNVEG VFCVTIIGT VLSCVLVFV EMAVHTFKK 
      SLRVKKPFK VLLMDEMRF YFRTSAMLK PSVKSRKRK 
      NRLVPCLST MRCLGLTVF LLIFPNFLG QEQDRKEIF 
      LGGIFTEPP DVDDSVLSD EEAFNFAID IANREYSDV 
      KFTSVSEES DLRTNGPFD SRLQACSLV NQQALVIFG 
      PKNAEEIDI VQSICDNKD LAHVITRWV YSSADFRSV 
      INFYPHSAY LTSAYFSVL KLWNWKTLT VFYEDNESM 
      LRLGDLLNL AKNEGIIVT VKQLYEGLD ETPIYRTTL 
      KEAVRSGQK NFIIDCKIE SLEEVLKQA QQVGLMTKD 
      YNFFITNLD LQTINLEPF QYSEANITG IRILDPLNE 
      MFHVKAGAI MRQKPNFNL TKMRTETAL LIDAVSVIT 
      QVISRKLSI KEMEMTEIS CNSPKSSRH GYTIANHVK 
      TSKFDEMTG RIEFDGNGV RSNFDLDVI ELTQNGISK 
      IGTWNMSKG LVITPHKDE DIVEDPLSL RNKTFKVIT 
      CLTDPYCML KENSGQLFG NDRFEGFAI DLIHELAQM 
      EGFNYTFII REDKSNGDK NKVTGEWSG MIGDVMHGV 
      ADLAITDLT ITAEREEAV DFTSPFMNL GISILAKKP 
      GNAPPSFFS FADPFALDT WIMLALAYI AVSVSFFVL 
      GRICPDEWT NPYPCVEEP EFLINQFSL SNSFWYAVG 
      SLMQQGTEL APIGVPTRM VAGMWWFFV LIMVSSYTA 
      SLAAFLANE NTITLFTDV ESLVQNYEE KGIRMGAKR 
      KGATEGFFR GKDSETYKI IAKYMEEHP DDMVGDNKD 
      GVKLANKET YAFFMESIS IEYETQRHC DLQQYGGLL 
      DDKGYGIAM RKNSTYRKT LSTAILKLQ SSGQLDNLK 
      RTWWEEKRG GGQCLDSGD DATPALDVR NVEGVFYVT 
      IGGTLCAIV LIFFELFLS LLKISKKYK ISMREALNN 
      EKKAFLDFN SNVKPAPKA KSKSGSKSS GESGKSNNN 
      TGAPTYGFI PTITKDTLD E
    CDS sequence
    • >TC031399 RA
      atgctgctg cttgtgatc acaatctca ctctatttt 
      cacaaattt tcccaagct gagacactc aaaatcgga 
      gctattttc gacaccgat gaccctatc aaagaacgc 
      gcatttcac cacgccatc catcaaatc gaacccatc 
      catgggcga accatcgaa ggcctggtc aaaaatgta 
      ccaccaaat gacccattt gaagccatg ctggcggct 
      tgtcacctc atcgaaagc ggagcggtt gcaattctg 
      ggtcccacg acccatgaa aacgcccat atggtccaa 
      accgtgtgc gataacaaa gacatccca ttgctggat 
      gtccgatct gttgcccat ccccaaaac tctatcaat 
      ttctaccct ttacaacaa atcctaacc cagatttat 
      atcaagttg ctcgaagct tggaatttc gaaaatttt 
      gtaattttg tatgaaaac gacgacagt ttgatcaga 
      ctcgccgaa ttgttgaaa ttctatgga aatgggcat 
      agaatggtc gtgagacaa cttgataag taccaaaat 
      ggtaattat aggccaaca ctgaaagaa gtgtggcga 
      tctggagca acacatttc gtcttagat tgttccact 
      gatatttta gaggaagtt ttgcatcaa gcccaacaa 
      gtgggcctt gtcactaac aaacagttt tacattatt 
      actaatttg gacttccac acgcttgat ttgacgtcg 
      tttcaatac tcggaaacg aacatcacc ggaatgcgg 
      tttattgat cctgatagt gacgagatt caaaattta 
      ggacttaca ctttaccga aatgacttc acaaacacc 
      gagtttggg tttatcgaa gcctggaag gttaattta 
      gagatggct ttgatcatt gatgctgtt acaatgttt 
      ggtgaagtc ctaaataga cttccgaaa gattttgca 
      ataccttcc atagactgt gcgtcggac aaagcttgg 
      acttacggg accacactc acaaattta gttaaatca 
      gttaagtac cccggttat acaggttta atccagttt 
      gataatttt ggacttcgc tcagctttc ggtctcgaa 
      atcattgaa ctgaaagaa ggtggaatt atcaaaatc 
      ggtaactgg aattactct gatgggtta aacattaac 
      cgggtttac ccacccgat ccccctccc cttgttgag 
      ggtagtcta gtaaatagg actttcatc gtaattact 
      tgcttgaca gaaccatat ggtatgaga agagattca 
      gaagtacca ttatatggg aacgaacgt tacgaaggc 
      ttcgggatt gatttaata gcagaactg tcgaaaaaa 
      cttggtttc aattacact ttcatcatc cgcgaagac 
      aaaaaaaat ggggagttt gacgaaagt agcggcgaa 
      tggactggg atgattggt gacgttatt agtggcaaa 
      gccgatttg gcaatcact gatctgacc atcacctca 
      gaacgagaa tctgcagtt gatttcagt accaccttt 
      atgagttta ggaatcagc atcctgtac caaaaaccc 
      aaaaaagct cttcctagt tttttctcc tttgctgat 
      cctttctca ctcacagtt tggaaactc ctggcagct 
      gctttcttt ggtgcttca atagctttg tttattttg 
      ggccggatt tcgccaagt gagtggcaa aatccatac 
      ccatgtgtc gaagacgag tttttggtc aatcagttg 
      agtttgaga aactgtgtc tggttcatg gtcggaagc 
      ttgatgcaa caaggatca gaaatcgcc ccaatagct 
      ttttcgaca cggatggtc gctggaatg tggtggttt 
      tttactttg atcatggtg tcgtcttac acagccaat 
      ctagccgcg tttttgacc accgaaagt ccagatctt 
      ccgttcaag gacgtgttc gagttggta caagtggca 
      gaaaagaag gggatcaaa tttggggca aaaatcaac 
      ggctcgacg gaaaaattt ttcctggat tccaagcac 
      gtcgatgag taccaacaa atctacaaa tacatgaaa 
      aaccatgaa gatgaagta atggtcaat gataataaa 
      gacggggtc cataaagcc gaacacgaa gactatgcc 
      ttctttatg gaaaccacc tcgatcgag tatgagaca 
      caaagacgt tgcggcttg acctcggtt ggccactct 
      ctggacgag aaaggatac ggaattgcg atgcgaaaa 
      aactcctcg tacagaatg gccctaagc actgctatt 
      ttgaagctc caagaagag ggagtttta gccaaattg 
      aagcgaaaa tggtgggag gagcaacga ggaggtggc 
      ctttgcccg caaggagaa aaatcaacc gaaggcacc 
      cccctcaac ctcaaaaac gtggagggc gttttctgc 
      gtgacgata atcgggact gtcctttcc tgcgtccta 
      gttttcgtc gaaatggcc gtacacaca tttaagaaa 
      tcgctccgt gtcaaaaaa cccttcaaa gtgctacta 
      atggacgaa atgcggttc tatttcagg acttccgct 
      atgctcaag ccttcggtt aaatcgagg aaacgtaaa 
      aatagactc gtgccgtgt ctcagtaca atgcggtgc 
      ttgggtttg acggtgttt ttgctgatt tttcccaac 
      tttctgggc caggaacaa gaccggaag gaaattttt 
      ttgggcgga attttcacg gagcctccg gatgtggac 
      gactcggtt ttgagtgat gaggaggcg ttcaatttt 
      gccattgac atcgcaaat cgggaatat tccgatgtc 
      aaatttaca agtgtgagt gaggaaagt gacttgagg 
      accaatggc ccgtttgac tcccgactg caagcttgc 
      tcactggtt aaccagcaa gctttggtt atttttggc 
      cccaaaaac gccgaagag attgacatc gtccagtca 
      atttgcgac aacaaggac ttggcccac gttatcacc 
      cgatgggtg tacagctct gccgatttt cgcagcgtt 
      atcaacttc tacccacat tcagcctat ctgacgtct 
      gcctatttc agcgtcttg aagctatgg aactggaag 
      accttgacc gtgttttac gaagacaac gaaagcatg 
      ctgcgactt ggcgatctc ttaaaccta gctaaaaat 
      gaaggaatt atagtgaca gtgaagcag ctctacgaa 
      ggactggac gaaacacca atttataga acaacacta 
      aaggaagcc gtgcgctcg ggtcagaag aacttcatc 
      atcgattgc aaaatcgag tctttggaa gaagtccta 
      aagcaggcc caacaagtc gggttgatg accaaggac 
      tataacttt ttcatcaca aatttggac ctgcagacg 
      attaatttg gagccgttt caatacagc gaagccaac 
      atcactggg atccggatt ttggatccc ttgaacgaa 
      atgttccat gtcaaggct ggggcaatt atgcgccaa 
      aaacccaac ttcaacttg accaaaatg aggactgaa 
      acggctctt ttgatagac gcagtatct gtcattacc 
      caagttatc agtaggaaa ttatcaatc aaggagatg 
      gaaatgacc gaaattagt tgcaactcg cccaagtcg 
      tcccggcat ggctacacg attgccaac cacgtgaaa 
      acgagcaag tttgacgaa atgaccggt cggattgag 
      ttcgatggc aatggagtt cggagtaat ttcgatcta 
      gacgtaata gaactgacc caaaatgga atttccaaa 
      attggcact tggaacatg tctaaaggc ctcgttatc 
      acaccacac aaagacgag gatattgta gaggatccc 
      ctcagtctc agaaacaag actttcaaa gtcattacg 
      tgcttgact gatccctac tgtatgttg aaagaaaat 
      tcaggccaa ttgtttggt aacgaccgc tttgagggc 
      ttcgccatt gatttgatc cacgaattg gcacaaatg 
      gaaggcttc aattacacg ttcataatc cgggaagac 
      aagtcaaac ggtgataaa aataaagta actggagaa 
      tggtcagga atgatagga gacgtcatg cacggggtt 
      gctgatttg gccatcact gacctaaca atcacagca 
      gaacgcgag gaagctgtt gattttact tcacctttc 
      atgaatttg ggaattagt attttggcc aaaaagcca 
      ggaaacgcc cctcctagt tttttctcg ttcgccgac 
      cccttcgca cttgacacc tggatcatg ttggcgctg 
      gcttacata gccgtctct gtttccttc tttgttttg 
      ggcagaata tgtccagac gagtggaca aatccttat 
      ccttgtgtc gaagaaccc gagtttttg atcaaccag 
      ttcagtctt tccaactct ttctggtac gctgttgga 
      agtctgatg cagcaaggg acagaacta gcaccaatc 
      ggtgttcct acacgaatg gtggctgga atgtggtgg 
      tttttcgtt ttgatcatg gtgtcctca tacacggcc 
      agtttggcg gcttttcta gccaacgaa aacaccata 
      acccttttc acagatgtt gaaagtttg gtccaaaac 
      tacgaggag aaagggata agaatggga gcgaaacgc 
      aaaggagcc actgaagga tttttcaga gggaaggac 
      agtgaaact tacaaaatc attgcaaag tacatggag 
      gaacaccca gatgatatg gtcggtgat aataaagac 
      ggtgtcaag ttggcaaat aaggaaact tatgctttc 
      ttcatggaa tctatttcc atagaatac gaaacgcaa 
      cgtcattgc gacttgcaa caatacggc ggacttctg 
      gacgacaaa ggctacgga attgccatg agaaaaaat 
      tcaacctat aggaagact ttaagtacg gccattttg 
      aaactacaa tcgtccggc caactcgat aatctcaaa 
      cggacgtgg tgggaggag aaacgtgga ggagggcaa 
      tgccttgat tctggtgat gatgcaacc cctgccctg 
      gacgtccga aacgtcgag ggtgtgttt tatgtcacg 
      atcgggggc acactctgc gccattgtg ctgattttc 
      ttcgaactg ttcttgtcc ctcctcaaa ataagcaaa 
      aaatacaaa atctcgatg cgagaagcc ctcaacaac 
      gagaaaaaa gcgttcctc gatttcaac agcaacgta 
      aaaccagca cccaaagcc aaatccaag tccggaagc 
      aaatcatcg ggagagagt ggcaagtcg aataataac 
      accggtgcc cccacttat ggcttcata ccgaccata 
      accaaagac acacttgac gaataa
    mRNA sequence
    • >TC031399 RA
      cataaaaca caatgctgc tgcttgtga tcacaatct 
      cactctatt ttcacaaat tttcccaag ctgagacac 
      tcaaaatcg gagctattt tcgacaccg atgacccta 
      tcaaagaac gcgcatttc accacgcca tccatcaaa 
      tcgaaccca tccatgggc gaaccatcg aaggcctgg 
      tcaaaaatg taccaccaa atgacccat ttgaagcca 
      tgctggcgg cttgtcacc tcatcgaaa gcggagcgg 
      ttgcaattc tgggtccca cgacccatg aaaacgccc 
      atatggtcc aaaccgtgt gcgataaca aagacatcc 
      cattgctgg atgtccgat ctgttgccc atccccaaa 
      actctatca atttctacc ctttacaac aaatcctaa 
      cccagattt atatcaagt tgctcgaag cttggaatt 
      tcgaaaatt ttgtaattt tgtatgaaa acgacgaca 
      gtttgatca gactcgccg aattgttga aattctatg 
      gaaatgggc atagaatgg tcgtgagac aacttgata 
      agtaccaaa atggtaatt ataggccaa cactgaaag 
      aagtgtggc gatctggag caacacatt tcgtcttag 
      attgttcca ctgatattt tagaggaag ttttgcatc 
      aagcccaac aagtgggcc ttgtcacta acaaacagt 
      tttacatta ttactaatt tggacttcc acacgcttg 
      atttgacgt cgtttcaat actcggaaa cgaacatca 
      ccggaatgc ggtttattg atcctgata gtgacgaga 
      ttcaaaatt taggactta cactttacc gaaatgact 
      tcacaaaca ccgagtttg ggtttatcg aagcctgga 
      aggttaatt tagagatgg ctttgatca ttgatgctg 
      ttacaatgt ttggtgaag tcctaaata gacttccga 
      aagattttg caatacctt ccatagact gtgcgtcgg 
      acaaagctt ggacttacg ggaccacac tcacaaatt 
      tagttaaat cagttaagt accccggtt atacaggtt 
      taatccagt ttgataatt ttggacttc gctcagctt 
      tcggtctcg aaatcattg aactgaaag aaggtggaa 
      ttatcaaaa tcggtaact ggaattact ctgatgggt 
      taaacatta accgggttt acccacccg atccccctc 
      cccttgttg agggtagtc tagtaaata ggactttca 
      tcgtaatta cttgcttga cagaaccat atggtatga 
      gaagagatt cagaagtac cattatatg ggaacgaac 
      gttacgaag gcttcggga ttgatttaa tagcagaac 
      tgtcgaaaa aacttggtt tcaattaca ctttcatca 
      tccgcgaag acaaaaaaa atggggagt ttgacgaaa 
      gtagcggcg aatggactg ggatgattg gtgacgtta 
      ttagtggca aagccgatt tggcaatca ctgatctga 
      ccatcacct cagaacgag aatctgcag ttgatttca 
      gtaccacct ttatgagtt taggaatca gcatcctgt 
      accaaaaac ccaaaaaag ctcttccta gttttttct 
      cctttgctg atcctttct cactcacag tttggaaac 
      tcctggcag ctgctttct ttggtgctt caatagctt 
      tgtttattt tgggccgga tttcgccaa gtgagtggc 
      aaaatccat acccatgtg tcgaagacg agtttttgg 
      tcaatcagt tgagtttga gaaactgtg tctggttca 
      tggtcggaa gcttgatgc aacaaggat cagaaatcg 
      ccccaatag ctttttcga cacggatgg tcgctggaa 
      tgtggtggt tttttactt tgatcatgg tgtcgtctt 
      acacagcca atctagccg cgtttttga ccaccgaaa 
      gtccagatc ttccgttca aggacgtgt tcgagttgg 
      tacaagtgg cagaaaaga aggggatca aatttgggg 
      caaaaatca acggctcga cggaaaaat ttttcctgg 
      attccaagc acgtcgatg agtaccaac aaatctaca 
      aatacatga aaaaccatg aagatgaag taatggtca 
      atgataata aagacgggg tccataaag ccgaacacg 
      aagactatg ccttcttta tggaaacca cctcgatcg 
      agtatgaga cacaaagac gttgcggct tgacctcgg 
      ttggccact ctctggacg agaaaggat acggaattg 
      cgatgcgaa aaaactcct cgtacagaa tggccctaa 
      gcactgcta ttttgaagc tccaagaag agggagttt 
      tagccaaat tgaagcgaa aatggtggg aggagcaac 
      gaggaggtg gcctttgcc cgcaaggag aaaaatcaa 
      ccgaaggca cccccctca acctcaaaa acgtggagg 
      gcgttttct gcgtgacga taatcggga ctgtccttt 
      cctgcgtcc tagttttcg tcgaaatgg ccgtacaca 
      catttaaga aatcgctcc gtgtcaaaa aacccttca 
      aagtgctac taatggacg aaatgcggt tctatttca 
      ggacttccg ctatgctca agccttcgg ttaaatcga 
      ggaaacgta aaaatagac tcgtgccgt gtctcagta 
      caatgcggt gcttgggtt tgacggtgt ttttgctga 
      tttttccca actttctgg gccaggaac aagaccgga 
      aggaaattt ttttgggcg gaattttca cggagcctc 
      cggatgtgg acgactcgg ttttgagtg atgaggagg 
      cgttcaatt ttgccattg acatcgcaa atcgggaat 
      attccgatg tcaaattta caagtgtga gtgaggaaa 
      gtgacttga ggaccaatg gcccgtttg actcccgac 
      tgcaagctt gctcactgg ttaaccagc aagctttgg 
      ttatttttg gccccaaaa acgccgaag agattgaca 
      tcgtccagt caatttgcg acaacaagg acttggccc 
      acgttatca cccgatggg tgtacagct ctgccgatt 
      ttcgcagcg ttatcaact tctacccac attcagcct 
      atctgacgt ctgcctatt tcagcgtct tgaagctat 
      ggaactgga agaccttga ccgtgtttt acgaagaca 
      acgaaagca tgctgcgac ttggcgatc tcttaaacc 
      tagctaaaa atgaaggaa ttatagtga cagtgaagc 
      agctctacg aaggactgg acgaaacac caatttata 
      gaacaacac taaaggaag ccgtgcgct cgggtcaga 
      agaacttca tcatcgatt gcaaaatcg agtctttgg 
      aagaagtcc taaagcagg cccaacaag tcgggttga 
      tgaccaagg actataact ttttcatca caaatttgg 
      acctgcaga cgattaatt tggagccgt ttcaataca 
      gcgaagcca acatcactg ggatccgga ttttggatc 
      ccttgaacg aaatgttcc atgtcaagg ctggggcaa 
      ttatgcgcc aaaaaccca acttcaact tgaccaaaa 
      tgaggactg aaacggctc ttttgatag acgcagtat 
      ctgtcatta cccaagtta tcagtagga aattatcaa 
      tcaaggaga tggaaatga ccgaaatta gttgcaact 
      cgcccaagt cgtcccggc atggctaca cgattgcca 
      accacgtga aaacgagca agtttgacg aaatgaccg 
      gtcggattg agttcgatg gcaatggag ttcggagta 
      atttcgatc tagacgtaa tagaactga cccaaaatg 
      gaatttcca aaattggca cttggaaca tgtctaaag 
      gcctcgtta tcacaccac acaaagacg aggatattg 
      tagaggatc ccctcagtc tcagaaaca agactttca 
      aagtcatta cgtgcttga ctgatccct actgtatgt 
      tgaaagaaa attcaggcc aattgtttg gtaacgacc 
      gctttgagg gcttcgcca ttgatttga tccacgaat 
      tggcacaaa tggaaggct tcaattaca cgttcataa 
      tccgggaag acaagtcaa acggtgata aaaataaag 
      taactggag aatggtcag gaatgatag gagacgtca 
      tgcacgggg ttgctgatt tggccatca ctgacctaa 
      caatcacag cagaacgcg aggaagctg ttgatttta 
      cttcacctt tcatgaatt tgggaatta gtattttgg 
      ccaaaaagc caggaaacg cccctccta gttttttct 
      cgttcgccg accccttcg cacttgaca cctggatca 
      tgttggcgc tggcttaca tagccgtct ctgtttcct 
      tctttgttt tgggcagaa tatgtccag acgagtgga 
      caaatcctt atccttgtg tcgaagaac ccgagtttt 
      tgatcaacc agttcagtc tttccaact ctttctggt 
      acgctgttg gaagtctga tgcagcaag ggacagaac 
      tagcaccaa tcggtgttc ctacacgaa tggtggctg 
      gaatgtggt ggtttttcg ttttgatca tggtgtcct 
      catacacgg ccagtttgg cggcttttc tagccaacg 
      aaaacacca taacccttt tcacagatg ttgaaagtt 
      tggtccaaa actacgagg agaaaggga taagaatgg 
      gagcgaaac gcaaaggag ccactgaag gatttttca 
      gagggaagg acagtgaaa cttacaaaa tcattgcaa 
      agtacatgg aggaacacc cagatgata tggtcggtg 
      ataataaag acggtgtca agttggcaa ataaggaaa 
      cttatgctt tcttcatgg aatctattt ccatagaat 
      acgaaacgc aacgtcatt gcgacttgc aacaatacg 
      gcggacttc tggacgaca aaggctacg gaattgcca 
      tgagaaaaa attcaacct ataggaaga ctttaagta 
      cggccattt tgaaactac aatcgtccg gccaactcg 
      ataatctca aacggacgt ggtgggagg agaaacgtg 
      gaggagggc aatgccttg attctggtg atgatgcaa 
      cccctgccc tggacgtcc gaaacgtcg agggtgtgt 
      tttatgtca cgatcgggg gcacactct gcgccattg 
      tgctgattt tcttcgaac tgttcttgt ccctcctca 
      aaataagca aaaaataca aaatctcga tgcgagaag 
      ccctcaaca acgagaaaa aagcgttcc tcgatttca 
      acagcaacg taaaaccag cacccaaag ccaaatcca 
      agtccggaa gcaaatcat cgggagaga gtggcaagt 
      cgaataata acaccggtg cccccactt atggcttca 
      taccgacca taaccaaag acacacttg acgaataat 
      tgaattttc gtaaaagtg tcacaataa ttcatcact 
      gtcaaaaat tgtgcaatc gtctctggc ccacttttt 
      gccgaatta tagattcgt gagagacga gcatctttc 
      acaagtaca ttgttaacg tcgtttcaa tgaaattga 
      cgattgttg ctaaattcc acaatgaat tgaattggt 
      ttggaatcg cactcgttt cgaatttat ttgagcgaa 
      gacgtatca ttaaactaa ataatcacg aaagcgttc 
      taaaatgca ctttatgta aaattcaag gaattaata 
      agggaaatt aagtcggag gcatccaga cattgagaa 
      gatttcaac aaccaaatt acattgaaa cgaaaagta 
      tttcatacc tcgttaagt tatttctga ttttatttt 
      tatttctag tcttaggac agtaaaaca aattcaatg 
      gaagcgtag aagggtctc acttttttt tcaaattat 
      ttgctaaat gtatacgtt tacccttct aagctttct 
      tatagtcct tttttatta acccatatt aaagattta 
      gtgtaaac
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