Status
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Gene information
General information
TC031393
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011392
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450233.1: 676569..713982
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031393
    iBeetle screen iB_05027

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031393 RA
      MTDDLCSGT PSRKRRTRT EMMRLLGMR LVTRLLLLV 
      CSLSLISLL FLSRCGLSG LETGAVVDE TEAQPVVSE 
      SAKKSIKRA DNLAKMCKM WGKVFLLGS CLLISSVPA 
      QNTSLELQQ GQNLDSLLS TLRNDALNP VSGLLGGAS 
      QVLQILGSG IQSLLLVLN VGVDKINII VLATLGTML 
      DKFFTTMQE VPVTLLNVL TRLVPDFLG GKSLDQFYM 
      KQEKCNRIF SRPLILEGV TKAAACINY YLNQIVSQL 
      VVVLEDMKK ILKILSDAL LELKEDSLG EMPKTVGRF 
      FESEGWKIV KSIVGDLPQ PFISLARAI PGLVICAVN 
      IVVTFARQV LGQVVPHLK STKLSSCLS ECKMSRPTL 
      IIFMTVLVF SSGVELPVK LPVISTQGL IPVSGLLGG 
      LAKILGNVI EALNAIIAY LNISLDGIV STVIALIGE 
      ILQDLVDTI NTTVSNIVA FLGKLPLIG EVFSDLDSK 
      SHECVALVK TNVFLVNAA PSTQCVIKF LDPTVAKLQ 
      DVLKKLREV LNSVTLLLN ELKTKSLVD ALSGLADGI 
      IQQLKMLVD AVVDLVVFI VKLLTKDAK TLMCVMKVI 
      MKLVGTLLS EIFGYLSCL LASCSSECK MLRPTLIIF 
      VTVLVLSSG VELPVKLPV ISHQGLIPV SGLLGGLAK 
      ILGNVIEAL NAIIAYLSI GISGIIATV FGLIGEILQ 
      DLLETIIAT ISNIVGVNA GSASQCVTE ALDQIVDKL 
      QDVLKQVRD VLNSVTLLL NGLIGEPIT AAVSGLLDG 
      IIEQVKMII EAIVDLVIF IVNMLMEDG NAIFCLLKV 
      VLKLVGQLV SEIFGYVGC LLGSTVIAN QVVSHTANS 
      DGLSYQQLL QQREEENRQ EVERLTSEI RALKLQLLQ 
      LTNGNSRPF TLNAPPNTS TTPLSNTDC ANFIKKQAM 
      SAEIFHGIP LNNEYELIP FNHFTYSRV YPLELGLGK 
      RVVEKPIGF KRKDLLESL MKAIETLNK NTTDKLNKY 
      TLDDFLEGL YRTEPTTGT QYELYFKTR GPKKGAQGG 
      FTKVVVFRP FAPVQTVAT EGLMGPRDK PLIHIILPL 
      SGRTTTFQS FMDKFVKIG LKNDRRVHL TVVYFGEEG 
      LSEARSIMS RVLMTKNSG GNANNLRLL ALNETFSRG 
      KGLRVGAER LWGDRKDVL LFMCDVDVV FSARFLDRC 
      RWNTKAGRK VYYPVVFSL YNPHVVYTL QGREVPPEN 
      DQLVISRDT GFWRDFGYG MTCQYRSDF LKVRGFDED 
      IVGWGGEDV MLYRKYVRS NMKVIRATD PGIFHIWHP 
      KVCTGGSQK LSADQYRAC IRSRALNEA SHAQLGFLA 
      FRDDIAANS SPVKYPSKA LPSRTIA
    CDS sequence
    • >TC031393 RA
      atgacagat gatctttgc agtggaact cctagcagg 
      aagcggcgg acacgtacc gagatgatg cgtcttctc 
      ggcatgaga cttgtaaca agacttctg ctgcttgtt 
      tgctcgctc agtctaatc agcttgttg ttcctgagc 
      aggtgcgga ctgagcggg ctcgaaacg ggggctgtg 
      gtggatgag acggaggca cagcctgtt gtttcagaa 
      agtgcaaaa aaaagtata aaacgcgcc gataatttg 
      gcaaaaatg tgcaaaatg tggggtaaa gtcttcctt 
      cttggcagc tgtttgctc atttcgtcg gtgcctgcc 
      caaaacacc tcattggag ctccaacaa gggcaaaac 
      ctcgattcc ttgctctca actcttcga aatgacgcc 
      ctgaaccct gttagtggc cttcttgga ggcgcctca 
      caagtttta caaattttg ggttcaggg atccaatcc 
      cttttgctc gttttgaac gtcggagtg gacaaaatc 
      aacataatc gtccttgca acactcggc acaatgctg 
      gacaagttt tttacaaca atgcaggaa gttccggtc 
      acacttttg aacgttttg actcgactt gtacccgat 
      tttttgggc gggaaaagt ctggatcag ttctacatg 
      aaacaggag aagtgtaat aggattttt tcgcggcct 
      ttgattctc gaaggggtt acgaaagct gccgcttgt 
      attaactac tacttgaac cagattgtg tcgcagttg 
      gttgtggtt cttgaagat atgaagaaa attttgaaa 
      attctctcc gatgcgctg ttagagttg aaggaagat 
      tcgttgggg gaaatgccg aagacggtt gggaggttt 
      ttcgagtct gaggggtgg aaaattgta aagtcgatt 
      gtcggggat ttaccgcaa ccttttatt agtttggcg 
      agagcaatt ccgggtctg gtgatctgt gcggttaat 
      attgtggtc acttttgcg aggcaggtt ctgggacag 
      gtggtccca catttgaaa tccaccaaa cttagctcg 
      tgtcttagt gagtgcaaa atgtcgcgt ccaacattg 
      ataattttt atgactgtt ctagtcttc agttccgga 
      gtggaactt ccagtcaaa cttccagtt atttccacc 
      caaggctta attccagtc agtggtctt cttggtggc 
      ttggccaaa attttgggc aatgttatc gaagctctg 
      aatgcaatt atcgcctat cttaatata agccttgat 
      gggatcgta tcaacagtt atagccctt ataggcgaa 
      attctgcaa gatttagtg gacactatt aacaccaca 
      gtgtccaac attgtggct ttcttgggc aaacttcca 
      ctcatcggt gaagttttc tccgattta gactccaaa 
      agccacgaa tgtgttgca ttagttaaa accaacgtc 
      ttcttagta aacgcagct ccatcaaca caatgcgtt 
      ataaaattc ctggacccc acagttgct aaactacag 
      gacgttttg aagaaactc cgcgaagtt cttaattct 
      gtgacactc ctccttaac gagctcaag acaaaatcg 
      cttgttgac gcactttca ggccttgca gatggtatt 
      attcaacaa ttgaagatg cttgttgat gctgttgtt 
      gatctggtg gtgtttatc gtaaaactg ctgaccaaa 
      gatgcaaaa acactaatg tgtgttatg aaagttatt 
      atgaagctt gttggaaca ctgctgtct gagattttt 
      ggttatttg agctgtctt ttggcctcg tgttctagt 
      gagtgcaag atgttgcgt ccaacttta ataattttt 
      gtgactgtt ctagtcctc agttctgga gtagaactt 
      ccagtaaaa cttccagtt atctcacac caagggctg 
      atcccagtc agcggtctc cttggaggc ttggccaaa 
      attttgggc aatgttatc gaagctctg aatgcaatt 
      attgcctac cttagcatt ggcatatct gggattata 
      gcaacagtt tttggtctc attggtgaa attctgcaa 
      gacttgctg gagaccatc attgccacc atctccaac 
      attgtggga gtgaacgca ggctcagcc tcacaatgc 
      gttaccgaa gctctggac caaattgtc gacaaatta 
      caagacgtt ttaaagcaa gttcgtgat gtcctcaac 
      tccgtcaca cttctcctc aatggtctc ataggagaa 
      ccaatcaca gccgccgtt tctggcctc cttgacgga 
      attatcgaa caagtcaag atgattatt gaggccatt 
      gttgatttg gtgatattt atcgtaaat atgctgatg 
      gaggatgga aatgccata ttctgtctt ttgaaagtt 
      gttctgaaa cttgtgggg caactagtt tctgagatt 
      tttggttat gtgggttgt cttttgggt tcgactgtg 
      atagcaaac caagtcgta agtcacaca gccaactct 
      gatggcctc tcctaccag caactcctc caacagcgt 
      gaagaagag aatcgtcaa gaagtggaa cgtttaaca 
      tccgaaatt cgtgcttta aaactccaa cttttgcag 
      cttacaaat ggtaattct cgccccttc accctcaac 
      gctcctccc aacacctcc accacccca ttatcaaac 
      accgactgc gccaacttt atcaaaaaa caggcaatg 
      tcagcggaa atcttccac ggcatcccc ttgaacaac 
      gaatacgaa ctaatacca ttcaaccac ttcacctac 
      agcagagtt tatcctcta gaacttggc ctcggcaaa 
      cgagttgtt gaaaaaccg atcggtttc aaacgtaaa 
      gacctcctc gaaagcctg atgaaggcc atcgaaacg 
      ctcaacaaa aacaccacc gacaaactc aacaaatac 
      acacttgac gacttcctg gaaggctta tatcgaact 
      gaacccaca actggaacc caatacgag ctctatttc 
      aagacgcga ggccccaaa aagggggcc cagggcggc 
      ttcaccaag gtcgtggtg ttccggccc ttcgccccc 
      gtgcagacg gtggccacc gaggggctc atgggcccc 
      cgagacaag cccctcatc cacatcatc ctgccattg 
      tcaggccgc acaacaacc ttccaaagc ttcatggac 
      aagtttgtt aaaatcgga ctgaagaac gacagacgt 
      gtgcacttg actgttgtc tattttggg gaagaaggc 
      ctgtctgaa gctcgttcg atcatgtct cgtgtttta 
      atgacgaaa aacagtggc ggtaatgcg aataatttg 
      cgtctgttg gcgctgaat gagacgttt tcgagggga 
      aaggggctg cgtgtgggg gcggagcgc ctttggggc 
      gatcgcaag gacgtgttg ttgttcatg tgtgatgtg 
      gatgtggtg ttcagtgcc aggtttttg gataggtgt 
      cggtggaat actaaagct gggaggaag gtttattat 
      ccggtggtg tttagtttg tacaacccc catgtggtg 
      tatacattg caagggagg gaggtgcct ccggagaat 
      gatcagttg gtgatttcg agggatacg gggttttgg 
      agggatttc gggtacggg atgacttgt cagtacagg 
      tctgacttt ctgaaggtg cgaggtttc gacgaggac 
      atagtaggc tggggtggg gaggacgtc atgctgtac 
      cggaagtac gtccggagc aacatgaaa gtaatccgg 
      gcaacggat cccggaatt tttcacata tggcacccc 
      aaggtgtgt acggggggt tcccagaag ctttccgcc 
      gaccagtac cgggcttgc atccgttcg cgggccctc 
      aacgaggcg tcccacgcc cagctgggc ttcctggcc 
      ttccgggac gacattgcc gccaactcc agccctgta 
      aagtacccc agcaaggcc cttccgagt aggacaatc 
      gcttaa
    mRNA sequence
    • >TC031393 RA
      tcgtcctga aatctggct tccacatta ttcagacga 
      acgccgaga ccgaatcca catatgaca gatgatctt 
      tgcagtgga actcctagc aggaagcgg cggacacgt 
      accgagatg atgcgtctt ctcggcatg agacttgta 
      acaagactt ctgctgctt gtttgctcg ctcagtcta 
      atcagcttg ttgttcctg agcaggtgc ggactgagc 
      gggctcgaa acgggggct gtggtggat gagacggag 
      gcacagcct gttgtttca gaaagtgca aaaaaaagt 
      ataaaacgc gccgataat ttggcaaaa atgtgcaaa 
      atgtggggt aaagtcttc cttcttggc agctgtttg 
      ctcatttcg tcggtgcct gcccaaaac acctcattg 
      gagctccaa caagggcaa aacctcgat tccttgctc 
      tcaactctt cgaaatgac gccctgaac cctgttagt 
      ggccttctt ggaggcgcc tcacaagtt ttacaaatt 
      ttgggttca gggatccaa tcccttttg ctcgttttg 
      aacgtcgga gtggacaaa atcaacata atcgtcctt 
      gcaacactc ggcacaatg ctggacaag ttttttaca 
      acaatgcag gaagttccg gtcacactt ttgaacgtt 
      ttgactcga cttgtaccc gattttttg ggcgggaaa 
      agtctggat cagttctac atgaaacag gagaagtgt 
      aataggatt ttttcgcgg cctttgatt ctcgaaggg 
      gttacgaaa gctgccgct tgtattaac tactacttg 
      aaccagatt gtgtcgcag ttggttgtg gttcttgaa 
      gatatgaag aaaattttg aaaattctc tccgatgcg 
      ctgttagag ttgaaggaa gattcgttg ggggaaatg 
      ccgaagacg gttgggagg tttttcgag tctgagggg 
      tggaaaatt gtaaagtcg attgtcggg gatttaccg 
      caacctttt attagtttg gcgagagca attccgggt 
      ctggtgatc tgtgcggtt aatattgtg gtcactttt 
      gcgaggcag gttctggga caggtggtc ccacatttg 
      aaatccacc aaacttagc tcgtgtctt agtgagtgc 
      aaaatgtcg cgtccaaca ttgataatt tttatgact 
      gttctagtc ttcagttcc ggagtggaa cttccagtc 
      aaacttcca gttatttcc acccaaggc ttaattcca 
      gtcagtggt cttcttggt ggcttggcc aaaattttg 
      ggcaatgtt atcgaagct ctgaatgca attatcgcc 
      tatcttaat ataagcctt gatgggatc gtatcaaca 
      gttatagcc cttataggc gaaattctg caagattta 
      gtggacact attaacacc acagtgtcc aacattgtg 
      gctttcttg ggcaaactt ccactcatc ggtgaagtt 
      ttctccgat ttagactcc aaaagccac gaatgtgtt 
      gcattagtt aaaaccaac gtcttctta gtaaacgca 
      gctccatca acacaatgc gttataaaa ttcctggac 
      cccacagtt gctaaacta caggacgtt ttgaagaaa 
      ctccgcgaa gttcttaat tctgtgaca ctcctcctt 
      aacgagctc aagacaaaa tcgcttgtt gacgcactt 
      tcaggcctt gcagatggt attattcaa caattgaag 
      atgcttgtt gatgctgtt gttgatctg gtggtgttt 
      atcgtaaaa ctgctgacc aaagatgca aaaacacta 
      atgtgtgtt atgaaagtt attatgaag cttgttgga 
      acactgctg tctgagatt tttggttat ttgagctgt 
      cttttggcc tcgtgttct agtgagtgc aagatgttg 
      cgtccaact ttaataatt tttgtgact gttctagtc 
      ctcagttct ggagtagaa cttccagta aaacttcca 
      gttatctca caccaaggg ctgatccca gtcagcggt 
      ctccttgga ggcttggcc aaaattttg ggcaatgtt 
      atcgaagct ctgaatgca attattgcc taccttagc 
      attggcata tctgggatt atagcaaca gtttttggt 
      ctcattggt gaaattctg caagacttg ctggagacc 
      atcattgcc accatctcc aacattgtg ggagtgaac 
      gcaggctca gcctcacaa tgcgttacc gaagctctg 
      gaccaaatt gtcgacaaa ttacaagac gttttaaag 
      caagttcgt gatgtcctc aactccgtc acacttctc 
      ctcaatggt ctcatagga gaaccaatc acagccgcc 
      gtttctggc ctccttgac ggaattatc gaacaagtc 
      aagatgatt attgaggcc attgttgat ttggtgata 
      tttatcgta aatatgctg atggaggat ggaaatgcc 
      atattctgt cttttgaaa gttgttctg aaacttgtg 
      gggcaacta gtttctgag atttttggt tatgtgggt 
      tgtcttttg ggttcgact gtgatagca aaccaagtc 
      gtaagtcac acagccaac tctgatggc ctctcctac 
      cagcaactc ctccaacag cgtgaagaa gagaatcgt 
      caagaagtg gaacgttta acatccgaa attcgtgct 
      ttaaaactc caacttttg cagcttaca aatggtaat 
      tctcgcccc ttcaccctc aacgctcct cccaacacc 
      tccaccacc ccattatca aacaccgac tgcgccaac 
      tttatcaaa aaacaggca atgtcagcg gaaatcttc 
      cacggcatc cccttgaac aacgaatac gaactaata 
      ccattcaac cacttcacc tacagcaga gtttatcct 
      ctagaactt ggcctcggc aaacgagtt gttgaaaaa 
      ccgatcggt ttcaaacgt aaagacctc ctcgaaagc 
      ctgatgaag gccatcgaa acgctcaac aaaaacacc 
      accgacaaa ctcaacaaa tacacactt gacgacttc 
      ctggaaggc ttatatcga actgaaccc acaactgga 
      acccaatac gagctctat ttcaagacg cgaggcccc 
      aaaaagggg gcccagggc ggcttcacc aaggtcgtg 
      gtgttccgg cccttcgcc cccgtgcag acggtggcc 
      accgagggg ctcatgggc ccccgagac aagcccctc 
      atccacatc atcctgcca ttgtcaggc cgcacaaca 
      accttccaa agcttcatg gacaagttt gttaaaatc 
      ggactgaag aacgacaga cgtgtgcac ttgactgtt 
      gtctatttt ggggaagaa ggcctgtct gaagctcgt 
      tcgatcatg tctcgtgtt ttaatgacg aaaaacagt 
      ggcggtaat gcgaataat ttgcgtctg ttggcgctg 
      aatgagacg ttttcgagg ggaaagggg ctgcgtgtg 
      ggggcggag cgcctttgg ggcgatcgc aaggacgtg 
      ttgttgttc atgtgtgat gtggatgtg gtgttcagt 
      gccaggttt ttggatagg tgtcggtgg aatactaaa 
      gctgggagg aaggtttat tatccggtg gtgtttagt 
      ttgtacaac ccccatgtg gtgtataca ttgcaaggg 
      agggaggtg cctccggag aatgatcag ttggtgatt 
      tcgagggat acggggttt tggagggat ttcgggtac 
      gggatgact tgtcagtac aggtctgac tttctgaag 
      gtgcgaggt ttcgacgag gacatagta ggctggggt 
      ggggaggac gtcatgctg taccggaag tacgtccgg 
      agcaacatg aaagtaatc cgggcaacg gatcccgga 
      atttttcac atatggcac cccaaggtg tgtacgggg 
      ggttcccag aagctttcc gccgaccag taccgggct 
      tgcatccgt tcgcgggcc ctcaacgag gcgtcccac 
      gcccagctg ggcttcctg gccttccgg gacgacatt 
      gccgccaac tccagccct gtaaagtac cccagcaag 
      gcccttccg agtaggaca atcgcttaa aaggacaca 
      cacagtgat tcgaaaaat tcaacattc ctgttgtgt 
      gactgtaga aatttgttt gccaaaaaa gccacttga 
      tgtgattga agccgaaaa attccaagt gatgatgat 
      attttgtta tgtttgtaa gactgttat tggctacat 
      cgaaatagt tttaagtag atttggccg tcaaaggta 
      gactagtcc tagtataat acctacctg cattgagtg 
      aaagatgaa accttttgt atttttaat taattaagg 
      cacgaaaat ggcgtccca cgacaaatt atatcatta 
      gagatgtat ttattgaga ttggttggg ttttggtgt 
      aaggccgaa ttttaaaaa taaatcaat tttgttgta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY