| General information | |||
| TC011379 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015450233.1: 480472..493572 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC011379 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05023 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
- >TC011379 RA
MKLFWLVTL PFALATPSF IPCSEISRD LRFPCTCSL GPPEPALDN NPAISVNCD RVVFPGDLP ALPFGAPIV SFRQRWAGH QALPTQIFS STGLPLRSI DLSGNSLRR LTERLLQAL QPTLVELRL SDNLLGDTL NPIFSTSEF RGLNHLQVL DLSGNIIRA LEEGIFEGC DNLQELYLE RNSLTSVPS TSLNGPKSL KMLSLASNR ITSLKSAAF EAQPNLETV DLTLNGIGG IEGGALSGL KNLKTLKLG HNKLTRFNS DVFQGASNL KNLDLSENF ITEFPTIAL RAFKDLKYL NLSSNLVQS LDNNDLLNL VGLYYLDLS RNNIANIAP GTFLGLKQL RKLDISVNS LRTIEDDAF EGLDNLEHL NLKDNNILL IPASALGRL PKLSSLQLD YNRVAALSG DILRSIAEK VTSLVLAKN VIRELPPAS FQHFQHLSH LDLTRNLLT TLNSDAFVG LETTLKELH LPQNKISTI TGPTLSLLK LETLDLSDN HLTELSRNV FGMLPQLRF LNLSHNSHL ASIPSNLLH KLPNLEVFD LSYTGLRIL TGDFFAKSS KLRRVYLHN NAISELGDG VFANLPNLT SIDLSSNHI NNIKQGAFV NIMNLKELV LRGNQLSSF KGEFFNTGT SLELLDISH NQLSYLFPS SFRIHPRLK ILKASNNKF NFFPAELIA TLQFLQVVD LSHNDLKTV EELDFARLP RLRVLLLRN NKLEFVSEM AFHNSTQLQ VIDLSYNKL ERLAERIFE GLVRLELLN LEGNLLSDL PETIFERAR LQMLENINL RGNLFEVAP LKSLQRQYF FVSSVDLSR NKLREIPGD DSSMVNIKK LDLSFNPLS DETIRNVLG EPKTMRELN LAGTGIREL TQLETPFLS YLNLSYNNI TSLNATIFE RTSLLEVLD VSNNQISDI SNYSNLWPL LKNLQTLDL SSNPIRSIS SSDFNGLTS LKQLSITSL LQCEKIEKG AFKSLKNLS ILKAFEFPK LGYIDLKGL LSHLPSLEH VTIETKDAA VGADQLQPL LQPRLKTLK LKGGRLRSL SSGTLSGIK GGEVTIGIM NTSITSLPP SLFFPVPRS SKITLDVTG NHITTLTPQ ILATLDDRR GDLKIVGLE TNPIVCDCG ARALRRWLP SHMTSLRCS APDHLHGRL LVEIADDDL TCDARKVTT PTSPLPRSA SSTRLHTKV TEPEIIWSV PVTEMVKTP KPVTSSNLN NDDTLIIGI VGGVVAFIA ILIIIICII RLRMGGSQY RGGPPVVGP LAGSSCACS VKGAPVYTM GPGYGPGYA ATLPHKMQA PIRASNYST MGRVPYYGN SGQPYFIAL PPDENKIYR
- >TC011379 RA
atgaagtta ttctggctc gtcaccctc cccttcgcc ctcgccacc ccctccttc atcccctgt tcggaaatc tcgcgcgat ttgcgcttc ccctgcact tgctccttg ggcccccct gagcccgcc ctggacaac aaccccgcc atcagcgtc aactgcgac cgggtcgtt ttccccggc gacctcccc gcccttccc ttcggcgcg ccaatcgtc tccttccgc caacgatgg gcggggcac caagccctc cccacccaa atcttctcc tcaacgggc ctcccctta cgcagcatc gatctatca ggcaactcc ctccggaga ctaacggaa cgcctcctc caagccctc cagccaacc ctagtggag ctccgcctc tcggacaac ctcctgggc gacaccctc aacccgatt ttctccacg agcgagttt cgcggtttg aatcatttg caagttttg gatttgagt gggaacatc atcagggcg ttggaggag gggattttt gaggggtgt gataatttg caagaactc tacttggag aggaatagt ctaacgagt gtgccaagc acgtcactc aacggaccc aagtcactc aaaatgtta tcactggcc tcaaatcgg atcacaagc ctcaaaagt gcagctttt gaggcacaa cctaacctt gaaacggtt gatttaacc ttgaatggg attggaggg atcgaaggt ggggctttg agtggcctc aaaaacctc aaaacactc aaattgggg cataacaag ttgacgagg ttcaacagc gatgttttt caaggagcc tcaaatctc aaaaatttg gacttgtcg gaaaatttt attacagag tttccgaca attgccctt agggccttc aaggattta aagtacctc aacttatcc tcaaattta gtccagagt ttggataat aatgattta ctcaatctt gtcggattg tattatttg gacttgagt cggaacaat attgccaac attgcacct ggaacattt ttgggatta aaacagttg aggaagctt gatattagt gtgaactcg ctccgtacg attgaggac gacgcattt gagggcctc gacaacttg gaacacttg aatttaaaa gacaacaac attttacta atcccagct tcggccctc ggccgtttg ccaaaacta tcttccctc cagttggac tataaccga gtggcagcc ctgtctggt gacattttg aggtcaata gccgagaaa gtcactagt ttagtcctc gccaaaaac gtcattcgt gaactcccc ccagcctcc ttccaacac ttccaacat ttatcacac ttagattta actcgcaac ctcctcaca accctcaat tcggacgca ttcgttggc cttgaaacc accctcaaa gagttacat ttacctcaa aacaaaatc tccacaatc actggaccc actctatcc ctccttaag ctcgaaacc ctcgattta agtgataat cacttgact gagctttcg cgcaacgta ttcgggatg ttacctcaa ttgaggttc ctcaatttg agtcacaac tcacaccta gcctcaata ccctcaaac ttgttacac aaactcccg aatttggag gtttttgat ttgagttac accggtttg aggatttta accggcgat tttttcgcc aagtcctca aaattgagg agggtttac ctccacaat aacgccatc agcgaactg ggcgatggt gtctttgcc aatttaccc aacttaacg tcaattgac ctctcctca aaccatatc aataacatt aaacaaggg gcgtttgtc aacatcatg aacctcaaa gagttggtt ttgaggggg aaccaattg agtagcttc aaaggcgag tttttcaac actgggaca agtctcgaa cttttggac atttcgcac aaccaattg agttatttg ttcccgtcc tctttcaga atacacccg cgcctcaaa atcctcaaa gcctcaaac aacaagttt aattttttc ccagctgag ttgatcgcg actttgcag tttttgcaa gttgttgat ttgtcacac aatgacctc aaaaccgtt gaggagttg gactttgcc cgcttgcca cgtttgagg gtcttactt ttgagaaac aataagttg gagtttgtt tccgaaatg gcgtttcat aactcaaca caactccaa gttatcgat ttgagttat aataagctc gaacgtctg gctgagagg atttttgag ggtttggtc cggttagag ttactcaat ttggagggg aaccttttg agtgattta cccgaaact atttttgaa agggcgagg ttacaaatg ttagagaac attaatttg agggggaac ttgtttgag gtagcgccc ctcaaaagt ctccaacgg cagtatttc tttgtctcg tcggttgat ttatcgagg aataaattg agggagatc ccgggggat gacagtagc atggttaac ataaaaaaa ctggacttg tcatttaat cccttgtct gatgaaacg attcgaaac gttttaggg gaacctaag accatgcgg gagctaaat ttggcggga accgggatt cgggaacta acccagctt gagaccccc tttttgagt tacctcaat ttgagttat aacaatata acctcactc aatgctaca attttcgaa cgaacttcc ctccttgaa gttctggac gtatcaaat aaccaaata agtgacatt tcaaattac tcaaacctt tggccttta ctcaaaaac ctccaaacc ctagacttg tcctcaaac cccatccgt agcatatct tcgagtgat ttcaatggc ttaacctcc ctcaaacaa ttgagtatc acgtccctc ctccaatgc gaaaaaatc gaaaaagga gcattcaaa tccctcaaa aacctgtca attctcaaa gcttttgag ttcccgaaa ttgggttac atcgacctc aaaggcctc ctctcgcac ctcccctcc ctcgaacac gtaaccata gaaacaaag gatgcggca gtgggcgcg gaccaactc cagcccctc ctccagccc cgcctcaaa accctcaaa ttaaaaggg gggcgctta cgcagccta tcatcggga accttgtca gggattaag ggaggagag gtcacaatc gggattatg aacacctca attacctcc ctcccccct tcactcttt ttccccgtc cctagatcc tcaaaaatc acactagac gtgactggc aatcacata actacttta actcctcaa attttggca acccttgat gatagacgt ggcgatttg aaaatagta ggccttgaa acaaatcct atagtttgt gactgtggc gcacgcgca ttgcgccga tggctcccc tcccatatg acgtcactg cgttgctcc gcccctgat cacttacac ggccgctta ttggtcgag atcgctgac gatgatctc acgtgtgat gccaggaag gtgaccacg cccacgtcg cccctccca cgttccgcc tcctcgacc cggttgcat acgaaagtg accgaaccg gagattatc tggtcggtt cccgtaacc gaaatggtt aaaaccccc aaacctgtt actagttca aatttgaat aatgacgat acgcttatt atcgggatc gtaggaggc gtggtcgcg ttcattgcc atcttgata attattatt tgtataatt cgactgagg atggggggg agtcagtac aggggcggg ccgcctgtt gtggggccc cttgcgggg agtagttgt gcctgtagt gtaaaaggg gcacctgtt tatacaatg gggcctggg tacgggcct ggatatgcg gcaacgttg ccacataaa atgcaagcg cctattaga gcgagtaat tactcgacg atgggcaga gtgccctat tatggcaac agtgggcag ccgtatttt attgcgttg ccgcctgat gaaaataaa atctatagg taa
- >TC011379 RA
catcgttgg taaaaaatg ggtagcgta tgtctgtac catcgatgg taaaatctc acctgttca gcctcctcc agtgtgaag gaggtgtcg aaggagtga atatatcgg tgtgtgtgt ttttgtgag ggtgttttg agggtggaa atgaagcag gattgattt ttggaggtg tttttttga ggataaaat gaagttatt ctggctcgt caccctccc cttcgccct cgccacccc ctccttcat cccctgttc ggaaatctc gcgcgattt gcgcttccc ctgcacttg ctccttggg cccccctga gcccgccct ggacaacaa ccccgccat cagcgtcaa ctgcgaccg ggtcgtttt ccccggcga cctccccgc ccttccctt cggcgcgcc aatcgtctc cttccgcca acgatgggc ggggcacca agccctccc cacccaaat cttctcctc aacgggcct ccccttacg cagcatcga tctatcagg caactccct ccggagact aacggaacg cctcctcca agccctcca gccaaccct agtggagct ccgcctctc ggacaacct cctgggcga caccctcaa cccgatttt ctccacgag cgagtttcg cggtttgaa tcatttgca agttttgga tttgagtgg gaacatcat cagggcgtt ggaggaggg gatttttga ggggtgtga taatttgca agaactcta cttggagag gaatagtct aacgagtgt gccaagcac gtcactcaa cggacccaa gtcactcaa aatgttatc actggcctc aaatcggat cacaagcct caaaagtgc agcttttga ggcacaacc taaccttga aacggttga tttaacctt gaatgggat tggagggat cgaaggtgg ggctttgag tggcctcaa aaacctcaa aacactcaa attggggca taacaagtt gacgaggtt caacagcga tgtttttca aggagcctc aaatctcaa aaatttgga cttgtcgga aaattttat tacagagtt tccgacaat tgcccttag ggccttcaa ggatttaaa gtacctcaa cttatcctc aaatttagt ccagagttt ggataataa tgatttact caatcttgt cggattgta ttatttgga cttgagtcg gaacaatat tgccaacat tgcacctgg aacattttt gggattaaa acagttgag gaagcttga tattagtgt gaactcgct ccgtacgat tgaggacga cgcatttga gggcctcga caacttgga acacttgaa tttaaaaga caacaacat tttactaat cccagcttc ggccctcgg ccgtttgcc aaaactatc ttccctcca gttggacta taaccgagt ggcagccct gtctggtga cattttgag gtcaatagc cgagaaagt cactagttt agtcctcgc caaaaacgt cattcgtga actcccccc agcctcctt ccaacactt ccaacattt atcacactt agatttaac tcgcaacct cctcacaac cctcaattc ggacgcatt cgttggcct tgaaaccac cctcaaaga gttacattt acctcaaaa caaaatctc cacaatcac tggacccac tctatccct ccttaagct cgaaaccct cgatttaag tgataatca cttgactga gctttcgcg caacgtatt cgggatgtt acctcaatt gaggttcct caatttgag tcacaactc acacctagc ctcaatacc ctcaaactt gttacacaa actcccgaa tttggaggt ttttgattt gagttacac cggtttgag gattttaac cggcgattt tttcgccaa gtcctcaaa attgaggag ggtttacct ccacaataa cgccatcag cgaactggg cgatggtgt ctttgccaa tttacccaa cttaacgtc aattgacct ctcctcaaa ccatatcaa taacattaa acaaggggc gtttgtcaa catcatgaa cctcaaaga gttggtttt gagggggaa ccaattgag tagcttcaa aggcgagtt tttcaacac tgggacaag tctcgaact tttggacat ttcgcacaa ccaattgag ttatttgtt cccgtcctc tttcagaat acacccgcg cctcaaaat cctcaaagc ctcaaacaa caagtttaa ttttttccc agctgagtt gatcgcgac tttgcagtt tttgcaagt tgttgattt gtcacacaa tgacctcaa aaccgttga ggagttgga ctttgcccg cttgccacg tttgagggt cttactttt gagaaacaa taagttgga gtttgtttc cgaaatggc gtttcataa ctcaacaca actccaagt tatcgattt gagttataa taagctcga acgtctggc tgagaggat ttttgaggg tttggtccg gttagagtt actcaattt ggaggggaa ccttttgag tgatttacc cgaaactat ttttgaaag ggcgaggtt acaaatgtt agagaacat taatttgag ggggaactt gtttgaggt agcgcccct caaaagtct ccaacggca gtatttctt tgtctcgtc ggttgattt atcgaggaa taaattgag ggagatccc gggggatga cagtagcat ggttaacat aaaaaaact ggacttgtc atttaatcc cttgtctga tgaaacgat tcgaaacgt tttagggga acctaagac catgcggga gctaaattt ggcgggaac cgggattcg ggaactaac ccagcttga gaccccctt tttgagtta cctcaattt gagttataa caatataac ctcactcaa tgctacaat tttcgaacg aacttccct ccttgaagt tctggacgt atcaaataa ccaaataag tgacatttc aaattactc aaacctttg gcctttact caaaaacct ccaaaccct agacttgtc ctcaaaccc catccgtag catatcttc gagtgattt caatggctt aacctccct caaacaatt gagtatcac gtccctcct ccaatgcga aaaaatcga aaaaggagc attcaaatc cctcaaaaa cctgtcaat tctcaaagc ttttgagtt cccgaaatt gggttacat cgacctcaa aggcctcct ctcgcacct cccctccct cgaacacgt aaccataga aacaaagga tgcggcagt gggcgcgga ccaactcca gcccctcct ccagccccg cctcaaaac cctcaaatt aaaaggggg gcgcttacg cagcctatc atcgggaac cttgtcagg gattaaggg aggagaggt cacaatcgg gattatgaa cacctcaat tacctccct ccccccttc actcttttt ccccgtccc tagatcctc aaaaatcac actagacgt gactggcaa tcacataac tactttaac tcctcaaat tttggcaac ccttgatga tagacgtgg cgatttgaa aatagtagg ccttgaaac aaatcctat agtttgtga ctgtggcgc acgcgcatt gcgccgatg gctcccctc ccatatgac gtcactgcg ttgctccgc ccctgatca cttacacgg ccgcttatt ggtcgagat cgctgacga tgatctcac gtgtgatgc caggaaggt gaccacgcc cacgtcgcc cctcccacg ttccgcctc ctcgacccg gttgcatac gaaagtgac cgaaccgga gattatctg gtcggttcc cgtaaccga aatggttaa aacccccaa acctgttac tagttcaaa tttgaataa tgacgatac gcttattat cgggatcgt aggaggcgt ggtcgcgtt cattgccat cttgataat tattatttg tataattcg actgaggat gggggggag tcagtacag gggcgggcc gcctgttgt ggggcccct tgcggggag tagttgtgc ctgtagtgt aaaaggggc acctgttta tacaatggg gcctgggta cgggcctgg atatgcggc aacgttgcc acataaaat gcaagcgcc tattagagc gagtaatta ctcgacgat gggcagagt gccctatta tggcaacag tgggcagcc gtattttat tgcgttgcc gcctgatga aaataaaat ctataggta accgtttta ggttaggtt taaggttat aatgtttga tttttattt aatgtttgc ttatttatg tataaatat aacttttat tacata
