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Gene information
General information
TC011379 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450233.1: 480472..493572
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011379
    iBeetle screen iB_05023

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011379 RA
      MKLFWLVTL PFALATPSF IPCSEISRD LRFPCTCSL 
      GPPEPALDN NPAISVNCD RVVFPGDLP ALPFGAPIV 
      SFRQRWAGH QALPTQIFS STGLPLRSI DLSGNSLRR 
      LTERLLQAL QPTLVELRL SDNLLGDTL NPIFSTSEF 
      RGLNHLQVL DLSGNIIRA LEEGIFEGC DNLQELYLE 
      RNSLTSVPS TSLNGPKSL KMLSLASNR ITSLKSAAF 
      EAQPNLETV DLTLNGIGG IEGGALSGL KNLKTLKLG 
      HNKLTRFNS DVFQGASNL KNLDLSENF ITEFPTIAL 
      RAFKDLKYL NLSSNLVQS LDNNDLLNL VGLYYLDLS 
      RNNIANIAP GTFLGLKQL RKLDISVNS LRTIEDDAF 
      EGLDNLEHL NLKDNNILL IPASALGRL PKLSSLQLD 
      YNRVAALSG DILRSIAEK VTSLVLAKN VIRELPPAS 
      FQHFQHLSH LDLTRNLLT TLNSDAFVG LETTLKELH 
      LPQNKISTI TGPTLSLLK LETLDLSDN HLTELSRNV 
      FGMLPQLRF LNLSHNSHL ASIPSNLLH KLPNLEVFD 
      LSYTGLRIL TGDFFAKSS KLRRVYLHN NAISELGDG 
      VFANLPNLT SIDLSSNHI NNIKQGAFV NIMNLKELV 
      LRGNQLSSF KGEFFNTGT SLELLDISH NQLSYLFPS 
      SFRIHPRLK ILKASNNKF NFFPAELIA TLQFLQVVD 
      LSHNDLKTV EELDFARLP RLRVLLLRN NKLEFVSEM 
      AFHNSTQLQ VIDLSYNKL ERLAERIFE GLVRLELLN 
      LEGNLLSDL PETIFERAR LQMLENINL RGNLFEVAP 
      LKSLQRQYF FVSSVDLSR NKLREIPGD DSSMVNIKK 
      LDLSFNPLS DETIRNVLG EPKTMRELN LAGTGIREL 
      TQLETPFLS YLNLSYNNI TSLNATIFE RTSLLEVLD 
      VSNNQISDI SNYSNLWPL LKNLQTLDL SSNPIRSIS 
      SSDFNGLTS LKQLSITSL LQCEKIEKG AFKSLKNLS 
      ILKAFEFPK LGYIDLKGL LSHLPSLEH VTIETKDAA 
      VGADQLQPL LQPRLKTLK LKGGRLRSL SSGTLSGIK 
      GGEVTIGIM NTSITSLPP SLFFPVPRS SKITLDVTG 
      NHITTLTPQ ILATLDDRR GDLKIVGLE TNPIVCDCG 
      ARALRRWLP SHMTSLRCS APDHLHGRL LVEIADDDL 
      TCDARKVTT PTSPLPRSA SSTRLHTKV TEPEIIWSV 
      PVTEMVKTP KPVTSSNLN NDDTLIIGI VGGVVAFIA 
      ILIIIICII RLRMGGSQY RGGPPVVGP LAGSSCACS 
      VKGAPVYTM GPGYGPGYA ATLPHKMQA PIRASNYST 
      MGRVPYYGN SGQPYFIAL PPDENKIYR
    CDS sequence
    • >TC011379 RA
      atgaagtta ttctggctc gtcaccctc cccttcgcc 
      ctcgccacc ccctccttc atcccctgt tcggaaatc 
      tcgcgcgat ttgcgcttc ccctgcact tgctccttg 
      ggcccccct gagcccgcc ctggacaac aaccccgcc 
      atcagcgtc aactgcgac cgggtcgtt ttccccggc 
      gacctcccc gcccttccc ttcggcgcg ccaatcgtc 
      tccttccgc caacgatgg gcggggcac caagccctc 
      cccacccaa atcttctcc tcaacgggc ctcccctta 
      cgcagcatc gatctatca ggcaactcc ctccggaga 
      ctaacggaa cgcctcctc caagccctc cagccaacc 
      ctagtggag ctccgcctc tcggacaac ctcctgggc 
      gacaccctc aacccgatt ttctccacg agcgagttt 
      cgcggtttg aatcatttg caagttttg gatttgagt 
      gggaacatc atcagggcg ttggaggag gggattttt 
      gaggggtgt gataatttg caagaactc tacttggag 
      aggaatagt ctaacgagt gtgccaagc acgtcactc 
      aacggaccc aagtcactc aaaatgtta tcactggcc 
      tcaaatcgg atcacaagc ctcaaaagt gcagctttt 
      gaggcacaa cctaacctt gaaacggtt gatttaacc 
      ttgaatggg attggaggg atcgaaggt ggggctttg 
      agtggcctc aaaaacctc aaaacactc aaattgggg 
      cataacaag ttgacgagg ttcaacagc gatgttttt 
      caaggagcc tcaaatctc aaaaatttg gacttgtcg 
      gaaaatttt attacagag tttccgaca attgccctt 
      agggccttc aaggattta aagtacctc aacttatcc 
      tcaaattta gtccagagt ttggataat aatgattta 
      ctcaatctt gtcggattg tattatttg gacttgagt 
      cggaacaat attgccaac attgcacct ggaacattt 
      ttgggatta aaacagttg aggaagctt gatattagt 
      gtgaactcg ctccgtacg attgaggac gacgcattt 
      gagggcctc gacaacttg gaacacttg aatttaaaa 
      gacaacaac attttacta atcccagct tcggccctc 
      ggccgtttg ccaaaacta tcttccctc cagttggac 
      tataaccga gtggcagcc ctgtctggt gacattttg 
      aggtcaata gccgagaaa gtcactagt ttagtcctc 
      gccaaaaac gtcattcgt gaactcccc ccagcctcc 
      ttccaacac ttccaacat ttatcacac ttagattta 
      actcgcaac ctcctcaca accctcaat tcggacgca 
      ttcgttggc cttgaaacc accctcaaa gagttacat 
      ttacctcaa aacaaaatc tccacaatc actggaccc 
      actctatcc ctccttaag ctcgaaacc ctcgattta 
      agtgataat cacttgact gagctttcg cgcaacgta 
      ttcgggatg ttacctcaa ttgaggttc ctcaatttg 
      agtcacaac tcacaccta gcctcaata ccctcaaac 
      ttgttacac aaactcccg aatttggag gtttttgat 
      ttgagttac accggtttg aggatttta accggcgat 
      tttttcgcc aagtcctca aaattgagg agggtttac 
      ctccacaat aacgccatc agcgaactg ggcgatggt 
      gtctttgcc aatttaccc aacttaacg tcaattgac 
      ctctcctca aaccatatc aataacatt aaacaaggg 
      gcgtttgtc aacatcatg aacctcaaa gagttggtt 
      ttgaggggg aaccaattg agtagcttc aaaggcgag 
      tttttcaac actgggaca agtctcgaa cttttggac 
      atttcgcac aaccaattg agttatttg ttcccgtcc 
      tctttcaga atacacccg cgcctcaaa atcctcaaa 
      gcctcaaac aacaagttt aattttttc ccagctgag 
      ttgatcgcg actttgcag tttttgcaa gttgttgat 
      ttgtcacac aatgacctc aaaaccgtt gaggagttg 
      gactttgcc cgcttgcca cgtttgagg gtcttactt 
      ttgagaaac aataagttg gagtttgtt tccgaaatg 
      gcgtttcat aactcaaca caactccaa gttatcgat 
      ttgagttat aataagctc gaacgtctg gctgagagg 
      atttttgag ggtttggtc cggttagag ttactcaat 
      ttggagggg aaccttttg agtgattta cccgaaact 
      atttttgaa agggcgagg ttacaaatg ttagagaac 
      attaatttg agggggaac ttgtttgag gtagcgccc 
      ctcaaaagt ctccaacgg cagtatttc tttgtctcg 
      tcggttgat ttatcgagg aataaattg agggagatc 
      ccgggggat gacagtagc atggttaac ataaaaaaa 
      ctggacttg tcatttaat cccttgtct gatgaaacg 
      attcgaaac gttttaggg gaacctaag accatgcgg 
      gagctaaat ttggcggga accgggatt cgggaacta 
      acccagctt gagaccccc tttttgagt tacctcaat 
      ttgagttat aacaatata acctcactc aatgctaca 
      attttcgaa cgaacttcc ctccttgaa gttctggac 
      gtatcaaat aaccaaata agtgacatt tcaaattac 
      tcaaacctt tggccttta ctcaaaaac ctccaaacc 
      ctagacttg tcctcaaac cccatccgt agcatatct 
      tcgagtgat ttcaatggc ttaacctcc ctcaaacaa 
      ttgagtatc acgtccctc ctccaatgc gaaaaaatc 
      gaaaaagga gcattcaaa tccctcaaa aacctgtca 
      attctcaaa gcttttgag ttcccgaaa ttgggttac 
      atcgacctc aaaggcctc ctctcgcac ctcccctcc 
      ctcgaacac gtaaccata gaaacaaag gatgcggca 
      gtgggcgcg gaccaactc cagcccctc ctccagccc 
      cgcctcaaa accctcaaa ttaaaaggg gggcgctta 
      cgcagccta tcatcggga accttgtca gggattaag 
      ggaggagag gtcacaatc gggattatg aacacctca 
      attacctcc ctcccccct tcactcttt ttccccgtc 
      cctagatcc tcaaaaatc acactagac gtgactggc 
      aatcacata actacttta actcctcaa attttggca 
      acccttgat gatagacgt ggcgatttg aaaatagta 
      ggccttgaa acaaatcct atagtttgt gactgtggc 
      gcacgcgca ttgcgccga tggctcccc tcccatatg 
      acgtcactg cgttgctcc gcccctgat cacttacac 
      ggccgctta ttggtcgag atcgctgac gatgatctc 
      acgtgtgat gccaggaag gtgaccacg cccacgtcg 
      cccctccca cgttccgcc tcctcgacc cggttgcat 
      acgaaagtg accgaaccg gagattatc tggtcggtt 
      cccgtaacc gaaatggtt aaaaccccc aaacctgtt 
      actagttca aatttgaat aatgacgat acgcttatt 
      atcgggatc gtaggaggc gtggtcgcg ttcattgcc 
      atcttgata attattatt tgtataatt cgactgagg 
      atggggggg agtcagtac aggggcggg ccgcctgtt 
      gtggggccc cttgcgggg agtagttgt gcctgtagt 
      gtaaaaggg gcacctgtt tatacaatg gggcctggg 
      tacgggcct ggatatgcg gcaacgttg ccacataaa 
      atgcaagcg cctattaga gcgagtaat tactcgacg 
      atgggcaga gtgccctat tatggcaac agtgggcag 
      ccgtatttt attgcgttg ccgcctgat gaaaataaa 
      atctatagg taa
    mRNA sequence
    • >TC011379 RA
      catcgttgg taaaaaatg ggtagcgta tgtctgtac 
      catcgatgg taaaatctc acctgttca gcctcctcc 
      agtgtgaag gaggtgtcg aaggagtga atatatcgg 
      tgtgtgtgt ttttgtgag ggtgttttg agggtggaa 
      atgaagcag gattgattt ttggaggtg tttttttga 
      ggataaaat gaagttatt ctggctcgt caccctccc 
      cttcgccct cgccacccc ctccttcat cccctgttc 
      ggaaatctc gcgcgattt gcgcttccc ctgcacttg 
      ctccttggg cccccctga gcccgccct ggacaacaa 
      ccccgccat cagcgtcaa ctgcgaccg ggtcgtttt 
      ccccggcga cctccccgc ccttccctt cggcgcgcc 
      aatcgtctc cttccgcca acgatgggc ggggcacca 
      agccctccc cacccaaat cttctcctc aacgggcct 
      ccccttacg cagcatcga tctatcagg caactccct 
      ccggagact aacggaacg cctcctcca agccctcca 
      gccaaccct agtggagct ccgcctctc ggacaacct 
      cctgggcga caccctcaa cccgatttt ctccacgag 
      cgagtttcg cggtttgaa tcatttgca agttttgga 
      tttgagtgg gaacatcat cagggcgtt ggaggaggg 
      gatttttga ggggtgtga taatttgca agaactcta 
      cttggagag gaatagtct aacgagtgt gccaagcac 
      gtcactcaa cggacccaa gtcactcaa aatgttatc 
      actggcctc aaatcggat cacaagcct caaaagtgc 
      agcttttga ggcacaacc taaccttga aacggttga 
      tttaacctt gaatgggat tggagggat cgaaggtgg 
      ggctttgag tggcctcaa aaacctcaa aacactcaa 
      attggggca taacaagtt gacgaggtt caacagcga 
      tgtttttca aggagcctc aaatctcaa aaatttgga 
      cttgtcgga aaattttat tacagagtt tccgacaat 
      tgcccttag ggccttcaa ggatttaaa gtacctcaa 
      cttatcctc aaatttagt ccagagttt ggataataa 
      tgatttact caatcttgt cggattgta ttatttgga 
      cttgagtcg gaacaatat tgccaacat tgcacctgg 
      aacattttt gggattaaa acagttgag gaagcttga 
      tattagtgt gaactcgct ccgtacgat tgaggacga 
      cgcatttga gggcctcga caacttgga acacttgaa 
      tttaaaaga caacaacat tttactaat cccagcttc 
      ggccctcgg ccgtttgcc aaaactatc ttccctcca 
      gttggacta taaccgagt ggcagccct gtctggtga 
      cattttgag gtcaatagc cgagaaagt cactagttt 
      agtcctcgc caaaaacgt cattcgtga actcccccc 
      agcctcctt ccaacactt ccaacattt atcacactt 
      agatttaac tcgcaacct cctcacaac cctcaattc 
      ggacgcatt cgttggcct tgaaaccac cctcaaaga 
      gttacattt acctcaaaa caaaatctc cacaatcac 
      tggacccac tctatccct ccttaagct cgaaaccct 
      cgatttaag tgataatca cttgactga gctttcgcg 
      caacgtatt cgggatgtt acctcaatt gaggttcct 
      caatttgag tcacaactc acacctagc ctcaatacc 
      ctcaaactt gttacacaa actcccgaa tttggaggt 
      ttttgattt gagttacac cggtttgag gattttaac 
      cggcgattt tttcgccaa gtcctcaaa attgaggag 
      ggtttacct ccacaataa cgccatcag cgaactggg 
      cgatggtgt ctttgccaa tttacccaa cttaacgtc 
      aattgacct ctcctcaaa ccatatcaa taacattaa 
      acaaggggc gtttgtcaa catcatgaa cctcaaaga 
      gttggtttt gagggggaa ccaattgag tagcttcaa 
      aggcgagtt tttcaacac tgggacaag tctcgaact 
      tttggacat ttcgcacaa ccaattgag ttatttgtt 
      cccgtcctc tttcagaat acacccgcg cctcaaaat 
      cctcaaagc ctcaaacaa caagtttaa ttttttccc 
      agctgagtt gatcgcgac tttgcagtt tttgcaagt 
      tgttgattt gtcacacaa tgacctcaa aaccgttga 
      ggagttgga ctttgcccg cttgccacg tttgagggt 
      cttactttt gagaaacaa taagttgga gtttgtttc 
      cgaaatggc gtttcataa ctcaacaca actccaagt 
      tatcgattt gagttataa taagctcga acgtctggc 
      tgagaggat ttttgaggg tttggtccg gttagagtt 
      actcaattt ggaggggaa ccttttgag tgatttacc 
      cgaaactat ttttgaaag ggcgaggtt acaaatgtt 
      agagaacat taatttgag ggggaactt gtttgaggt 
      agcgcccct caaaagtct ccaacggca gtatttctt 
      tgtctcgtc ggttgattt atcgaggaa taaattgag 
      ggagatccc gggggatga cagtagcat ggttaacat 
      aaaaaaact ggacttgtc atttaatcc cttgtctga 
      tgaaacgat tcgaaacgt tttagggga acctaagac 
      catgcggga gctaaattt ggcgggaac cgggattcg 
      ggaactaac ccagcttga gaccccctt tttgagtta 
      cctcaattt gagttataa caatataac ctcactcaa 
      tgctacaat tttcgaacg aacttccct ccttgaagt 
      tctggacgt atcaaataa ccaaataag tgacatttc 
      aaattactc aaacctttg gcctttact caaaaacct 
      ccaaaccct agacttgtc ctcaaaccc catccgtag 
      catatcttc gagtgattt caatggctt aacctccct 
      caaacaatt gagtatcac gtccctcct ccaatgcga 
      aaaaatcga aaaaggagc attcaaatc cctcaaaaa 
      cctgtcaat tctcaaagc ttttgagtt cccgaaatt 
      gggttacat cgacctcaa aggcctcct ctcgcacct 
      cccctccct cgaacacgt aaccataga aacaaagga 
      tgcggcagt gggcgcgga ccaactcca gcccctcct 
      ccagccccg cctcaaaac cctcaaatt aaaaggggg 
      gcgcttacg cagcctatc atcgggaac cttgtcagg 
      gattaaggg aggagaggt cacaatcgg gattatgaa 
      cacctcaat tacctccct ccccccttc actcttttt 
      ccccgtccc tagatcctc aaaaatcac actagacgt 
      gactggcaa tcacataac tactttaac tcctcaaat 
      tttggcaac ccttgatga tagacgtgg cgatttgaa 
      aatagtagg ccttgaaac aaatcctat agtttgtga 
      ctgtggcgc acgcgcatt gcgccgatg gctcccctc 
      ccatatgac gtcactgcg ttgctccgc ccctgatca 
      cttacacgg ccgcttatt ggtcgagat cgctgacga 
      tgatctcac gtgtgatgc caggaaggt gaccacgcc 
      cacgtcgcc cctcccacg ttccgcctc ctcgacccg 
      gttgcatac gaaagtgac cgaaccgga gattatctg 
      gtcggttcc cgtaaccga aatggttaa aacccccaa 
      acctgttac tagttcaaa tttgaataa tgacgatac 
      gcttattat cgggatcgt aggaggcgt ggtcgcgtt 
      cattgccat cttgataat tattatttg tataattcg 
      actgaggat gggggggag tcagtacag gggcgggcc 
      gcctgttgt ggggcccct tgcggggag tagttgtgc 
      ctgtagtgt aaaaggggc acctgttta tacaatggg 
      gcctgggta cgggcctgg atatgcggc aacgttgcc 
      acataaaat gcaagcgcc tattagagc gagtaatta 
      ctcgacgat gggcagagt gccctatta tggcaacag 
      tgggcagcc gtattttat tgcgttgcc gcctgatga 
      aaataaaat ctataggta accgtttta ggttaggtt 
      taaggttat aatgtttga tttttattt aatgtttgc 
      ttatttatg tataaatat aacttttat tacata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY