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Gene information
General information
TC011377 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450233.1: 409221..410888
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011377
    iBeetle screen iB_05022

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011377 RA
      MILRKVSQI RPLVIRFWL PVSRGCHTV APQLDLTYL 
      CDPQNIGEI SKNITNRKG IGNIERVHV LKKQLQELD 
      PNDKLYNAT KSEFETEVL RIPNRTHPQ VAGYGDSPK 
      TVEIVGTKP PDSSHLDFQ EICKKLRLL RTDQIGNFC 
      GSKSYYFLG ELAELEHAL VRYVTSKLL FNKFELVSV 
      PDILPRGTI EGCGMNTKG ARTQVYALD STLHGPDWC 
      LSGTSEMAL AGYLSNKTL NSDILPLKL AAVSRCYRA 
      ETSNLLEER GIYRVHEFT KVEMFVVTQ PQNSEQALE 
      DILKLEMDI FESLGLHFQ VLDMPPHEL GAQAYRKYD 
      IEAWMPGRG LYGEISSCS NCLDYQARR LGIRTHDGF 
      VHTLNGTAC AVPRLLIAL VEMGQRKNG TVGLPEVLW 
      PYMGGKQLI GKQKSVPDL RLIKTKKV
    CDS sequence
    • >TC011377 RA
      atgatttta cgaaaagtg agccaaatt cggccccta 
      gttatccgt ttttggctt ccggtgtct cggggctgt 
      cacacagtg gccccccaa ctcgatctc acctacttg 
      tgtgacccc caaaatata ggcgaaatt tcgaaaaac 
      atcactaat cggaaaggt atcggtaac atagagcgt 
      gtccatgtc ttgaagaag caactccag gagttggac 
      ccgaatgac aagctttac aacgcgacg aaaagtgaa 
      tttgagact gaagtgtta agaatcccc aatcggacg 
      caccctcag gtggctggt tatggtgat agccccaaa 
      acggtggaa atcgtgggg acgaagccc cccgattcc 
      tcccatctc gacttccag gagatttgt aagaagtta 
      aggctgctt cgtaccgac caaattggt aacttttgc 
      gggagcaaa agttactac tttctgggg gaattggca 
      gaattggag cacgctctt gttcgttac gtcacctcg 
      aaactgcta ttcaacaaa tttgagttg gtatcagtg 
      cctgatata ctcccaaga ggcacaatc gagggctgt 
      ggcatgaac accaaaggg gcccgcact caggtctac 
      gccctcgat agcaccctc cacggcccc gattggtgc 
      ctttcaggc acttcggaa atggcattg gccggttac 
      ctctcaaat aaaacccta aatagtgac attcttcca 
      ttgaagttg gcagcagtg agtcgttgc tatcgcgct 
      gaaacatcg aacctgctt gaagaacgg gggatttac 
      cgcgttcat gaattcact aaagttgaa atgttcgtt 
      gtaacgcaa ccccaaaac tcggaacaa gccttagaa 
      gatattctg aagttagaa atggacatt ttcgagtct 
      ttggggcta catttccaa gtcctggat atgcccccg 
      catgaattg ggggcgcag gcttatcgc aagtacgat 
      attgaggcg tggatgcct ggaaggggg ctgtatggg 
      gagatttcg agttgtagt aattgtctg gattatcag 
      gcaaggagg ctggggata cgcacccat gatgggttt 
      gttcatacg ttgaatggg acagcgtgt gcggttcca 
      aggctgctg atcgcgtta gtggagatg ggacagagg 
      aaaaatggg acagtgggg ctgccggag gtgctctgg 
      ccctacatg gggggcaag cagcttatt gggaagcaa 
      aaaagtgtt cccgacttg aggttaata aaaactaaa 
      aaagtttaa
    mRNA sequence
    • >TC011377 RA
      cctctgaaa cattaaata acacttttc gtcccttcg 
      ggacttcac caatccccg atgacgtaa ccacctgac 
      agctaacct cacaaaatc ataacaaaa aagcgatga 
      ttttacgaa aagtgagcc aaattcggc ccctagtta 
      tccgttttt ggcttccgg tgtctcggg gctgtcaca 
      cagtggccc cccaactcg atctcacct acttgtgtg 
      acccccaaa atataggcg aaatttcga aaaacatca 
      ctaatcgga aaggtatcg gtaacatag agcgtgtcc 
      atgtcttga agaagcaac tccaggagt tggacccga 
      atgacaagc tttacaacg cgacgaaaa gtgaatttg 
      agactgaag tgttaagaa tccccaatc ggacgcacc 
      ctcaggtgg ctggttatg gtgatagcc ccaaaacgg 
      tggaaatcg tggggacga agccccccg attcctccc 
      atctcgact tccaggaga tttgtaaga agttaaggc 
      tgcttcgta ccgaccaaa ttggtaact tttgcggga 
      gcaaaagtt actactttc tgggggaat tggcagaat 
      tggagcacg ctcttgttc gttacgtca cctcgaaac 
      tgctattca acaaatttg agttggtat cagtgcctg 
      atatactcc caagaggca caatcgagg gctgtggca 
      tgaacacca aaggggccc gcactcagg tctacgccc 
      tcgatagca ccctccacg gccccgatt ggtgccttt 
      caggcactt cggaaatgg cattggccg gttacctct 
      caaataaaa ccctaaata gtgacattc ttccattga 
      agttggcag cagtgagtc gttgctatc gcgctgaaa 
      catcgaacc tgcttgaag aacggggga tttaccgcg 
      ttcatgaat tcactaaag ttgaaatgt tcgttgtaa 
      cgcaacccc aaaactcgg aacaagcct tagaagata 
      ttctgaagt tagaaatgg acattttcg agtctttgg 
      ggctacatt tccaagtcc tggatatgc ccccgcatg 
      aattggggg cgcaggctt atcgcaagt acgatattg 
      aggcgtgga tgcctggaa gggggctgt atggggaga 
      tttcgagtt gtagtaatt gtctggatt atcaggcaa 
      ggaggctgg ggatacgca cccatgatg ggtttgttc 
      atacgttga atgggacag cgtgtgcgg ttccaaggc 
      tgctgatcg cgttagtgg agatgggac agaggaaaa 
      atgggacag tggggctgc cggaggtgc tctggccct 
      acatggggg gcaagcagc ttattggga agcaaaaaa 
      gtgttcccg acttgaggt taataaaaa ctaaaaaag 
      tttaatgat tgttttatt tgaacgtaa tcttaattc 
      ttcattttc ttcttcttc ttcctcttc ttcttcttt 
      ctcttcttc ctcttcttc ttctttctc ttctacctc 
      ttccacttt atcttctcc ctcttcttc cccttattc 
      ttccctttt tcttcttct atctatcta cagaagagc 
      tagataggg gaaaggcag agctaacga agagaatag 
      tttgagaaa agtagagct agagctagg gaagattag 
      aactagcca taacaagag cagagctga aaataaaaa 
      gagtaaagc tag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY