Status
Please wait ...
Gene information
General information
TC011361 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450233.1: 53816..55339
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011361
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05021

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011361 RA
      MSRGKVVLA YSGGLDTSC ILRWLLDQN YEVICYMAN 
      IGQDEDFEA ARRKAEQIG ASQVVIEDL REIFVKEYV 
      WPAIQAGLL YESRYLLGT SLARPCISV GLITAAKKY 
      NALYISHGA TGKGNDQVR FELSCYSLW PDVKIIAPW 
      RIKEFTSRF QGRQDLIKY ASLNNIPVP VTPKAPWSM 
      DANLMHVSY ESGILENPN NEPPHDLFH MTQDLAKTP 
      NEPFKLSIE FCQGLPVKV ITQDAQVFD KPLEMFTYL 
      NQIAGKHGI GRIDIVENR FIGLKSRGV YETPAGHIL 
      HVAHTDLEV FCLDKEVYR VKQNLKEKL SDFVYNGFW 
      FSPEGEYVR KCIALAEET VTGKVNMEV YKGNVVVLG 
      RWSPVSLYN EELVSMDKH SEFSPSDAT GFINIQAIR 
      LKEYHRFQK QQNK
    CDS sequence
    • >TC011361 RA
      atgagtcgc ggtaaagtc gttttggcc tattctggc 
      ggtttggac accagttgc atcctaaga tggctattg 
      gaccaaaac tacgaagtc atttgttac atggccaac 
      attggccaa gacgaagat ttcgaagct gcgaggcga 
      aaagccgag caaattggc gcatctcag gttgtgatt 
      gaagatttg agggagatt tttgtaaaa gaatacgtc 
      tggccggcc attcaagcc ggtctcttg tacgaatca 
      agatacctg cttggtact tctttggca cgaccttgt 
      atatcagta ggtttgata actgcggca aaaaaatat 
      aacgccctg tatatttct catggggct actggtaaa 
      ggcaacgac caagtcagg tttgaactg tcgtgttat 
      tccctctgg ccggatgtg aaaataatt gcgccttgg 
      aggatcaaa gagtttacg tcacggttt caaggccgt 
      caagacctt atcaaatat gcgtccttg aacaatatc 
      ccggtcccg gtgactccc aaggctccc tggagtatg 
      gatgccaac ctaatgcac gttagttac gaatcagga 
      attttggaa aaccctaac aatgaacca cctcatgac 
      cttttccac atgactcaa gacttggcc aagacgcct 
      aacgagccc tttaaactg tcaattgag ttctgtcaa 
      ggtctccca gtcaaggtc ataacccaa gatgcgcaa 
      gttttcgat aaaccgtta gaaatgttc acttatttg 
      aaccaaatc gcgggaaaa cacggcatt ggtcgcatt 
      gacatcgtc gagaaccgt ttcattggt ctaaagtca 
      cgtggtgtg tatgaaacg cccgctggg catatctta 
      cacgtggca cacaccgat ttggaggtt ttttgcctc 
      gataaggaa gtgtacaga gtgaagcag aatttgaaa 
      gagaagttg tccgatttt gtttataac gggttttgg 
      ttttcgccg gaaggtgaa tacgtgagg aaatgtatc 
      gcattggct gaggaaact gtcactggg aaagtcaat 
      atggaggtt tataaggga aatgttgtt gttttggga 
      cgttggtca cccgtttcg ctctataac gaggaactc 
      gtttcaatg gataaacat tccgagttt tcgccaagt 
      gacgccact gggtttatt aatattcaa gcgattagg 
      ttgaaggaa taccacagg tttcagaag caacaaaac 
      aaatga
    mRNA sequence
    • >TC011361 RA
      cagtttgtg acagaaatg agtcgcggt aaagtcgtt 
      ttggcctat tctggcggt ttggacacc agttgcatc 
      ctaagatgg ctattggac caaaactac gaagtcatt 
      tgttacatg gccaacatt ggccaagac gaagatttc 
      gaagctgcg aggcgaaaa gccgagcaa attggcgca 
      tctcaggtt gtgattgaa gatttgagg gagattttt 
      gtaaaagaa tacgtctgg ccggccatt caagccggt 
      ctcttgtac gaatcaaga tacctgctt ggtacttct 
      ttggcacga ccttgtata tcagtaggt ttgataact 
      gcggcaaaa aaatataac gccctgtat atttctcat 
      ggggctact ggtaaaggc aacgaccaa gtcaggttt 
      gaactgtcg tgttattcc ctctggccg gatgtgaaa 
      ataattgcg ccttggagg atcaaagag tttacgtca 
      cggtttcaa ggccgtcaa gaccttatc aaatatgcg 
      tccttgaac aatatcccg gtcccggtg actcccaag 
      gctccctgg agtatggat gccaaccta atgcacgtt 
      agttacgaa tcaggaatt ttggaaaac cctaacaat 
      gaaccacct catgacctt ttccacatg actcaagac 
      ttggccaag acgcctaac gagcccttt aaactgtca 
      attgagttc tgtcaaggt ctcccagtc aaggtcata 
      acccaagat gcgcaagtt ttcgataaa ccgttagaa 
      atgttcact tatttgaac caaatcgcg ggaaaacac 
      ggcattggt cgcattgac atcgtcgag aaccgtttc 
      attggtcta aagtcacgt ggtgtgtat gaaacgccc 
      gctgggcat atcttacac gtggcacac accgatttg 
      gaggttttt tgcctcgat aaggaagtg tacagagtg 
      aagcagaat ttgaaagag aagttgtcc gattttgtt 
      tataacggg ttttggttt tcgccggaa ggtgaatac 
      gtgaggaaa tgtatcgca ttggctgag gaaactgtc 
      actgggaaa gtcaatatg gaggtttat aagggaaat 
      gttgttgtt ttgggacgt tggtcaccc gtttcgctc 
      tataacgag gaactcgtt tcaatggat aaacattcc 
      gagttttcg ccaagtgac gccactggg tttattaat 
      attcaagcg attaggttg aaggaatac cacaggttt 
      cagaagcaa caaaacaaa tgaaggttt tcggagcca 
      acgagggaa ccaaattta ttttttcga aatataatg 
      tttgttttt tcataaaat aaagtttat aatataaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY