Status
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Gene information
General information
TC011356 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 4295589..4297188
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011356
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05020

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011356 RA
      MWPLLLLLL LQHIRPAHP SDAHVLSLS DTNFHRQLR 
      LNPTLLVQF FIPWSGMCQ KTRPHFARA AHILSTNQI 
      PVTLAKIDC SGRGRTTCT QKNITYPFP VFHFYRNGS 
      FVKEYTGSR DARSIVKFM RVQVVPNPV ELVDFEHFR 
      QFIEGQDDV IVVGFFEEE TKLRRIFFR VAEEMKESM 
      IFAYSSCEK VILKQGVSN GIVVFRPKS LHNQYEPER 
      VLFTGRSII GEIKNFITR NYHGLIGHR TPNNRHDFP 
      NPLVIVYHT IDYHSGGTE LNYWRYRLI KIAHKYQNF 
      VKIATSATD DFEDEISDL RTDNLTPTN PLVVAFDLE 
      NQKYVMVEP FTTSTFDNF VQNFINRKL TPYYRSKPI 
      PKENDGPVI IAVTQNFNH TVTNNGKDT LLDLYTPWS 
      LKCQKFVPV LREVAELLE HEDVAFVRM DAVENQVPE 
      VFNEKGIPN IFWLAKNRK RGPVVYEGE RSAEEVVKF 
      VAKKATKEL KNYDRKGNP KDDRDEL
    CDS sequence
    • >TC011356 RA
      atgtggccc ctcctcttg ctcctcctc ctccagcac 
      atccgtccc gcccatccc tccgacgcg cacgtcctc 
      agcctatcc gacaccaac ttccaccgc cagctccgc 
      ctcaacccc accctgctc gtccagttc ttcattccg 
      tggagcggc atgtgccaa aagacccgc ccccatttc 
      gcacgcgcc gcccacatt ctatccacc aaccaaatc 
      cccgtaact ctcgcaaaa atcgactgt tctggaagg 
      ggtcgcacg acatgtacc caaaaaaac atcacctac 
      ccctttccc gtgttccac ttctaccgt aacggaagc 
      ttcgtcaag gagtacacc gggtctaga gatgcccgc 
      agtattgtg aaatttatg cgcgttcaa gtggtgcca 
      aatccggtc gagttggtg gattttgag cactttcgg 
      cagtttatt gaagggcag gatgatgta attgttgtg 
      ggttttttc gaagaggag acgaagcta cggaggatt 
      tttttccgt gtcgcggag gagatgaaa gagagtatg 
      atttttgcg tactctagt tgtgagaag gtgattttg 
      aagcaaggt gttagtaat gggattgtg gtttttcgg 
      ccaaaaagt ctccacaat cagtatgaa ccagaacgg 
      gtacttttc acagggagg agtatcatt ggggagata 
      aaaaatttt attacacgg aactaccac ggtttaatt 
      ggccacaga acgcccaac aatcgacac gattttccc 
      aaccctttg gtcatcgtc taccacaca atcgactat 
      cactccggt ggtaccgaa ttaaattac tggcgctat 
      cgtttgatt aaaatcgcc cataagtac caaaacttc 
      gtcaaaatc gccacaagt gctaccgac gatttcgaa 
      gacgaaatt tccgacctc cggaccgat aacctcacc 
      cccactaac cccctggtt gtggcattt gacctcgag 
      aaccaaaaa tacgtcatg gtggaacct ttcaccacc 
      tcaactttt gataacttc gtccagaac ttcatcaac 
      cggaaactg actccctac tatcgctcc aaacccatc 
      cccaaggaa aacgacggt cctgtgatc attgccgtc 
      acccagaac ttcaaccac acggtgacc aacaacggc 
      aaagacacg cttctcgat ttgtacact ccgtggagt 
      ctcaaatgc cagaagttt gttccggtt ttgcgtgaa 
      gttgccgag cttttggag cacgaagat gtggctttt 
      gtccgaatg gatgccgtg gagaatcaa gtgcctgaa 
      gtgttcaac gagaaaggg ataccgaat attttctgg 
      ttggccaag aacaggaaa agggggccg gtggtgtac 
      gagggggag aggagtgcc gaggaagtt gtcaagttt 
      gtggcgaag aaggcgacc aaagagttg aagaattat 
      gataggaag gggaatccc aaggatgat cgagacgag 
      ttgtaa
    mRNA sequence
    • >TC011356 RA
      attttggta tttttattt tattttttt aatttttgg 
      gtgacatga caataacct caaaatcag ttcgaaacg 
      tcaaaattc ccgtcatgt ggcccctcc tcttgctcc 
      tcctcctcc agcacatcc gtcccgccc atccctccg 
      acgcgcacg tcctcagcc tatccgaca ccaacttcc 
      accgccagc tccgcctca accccaccc tgctcgtcc 
      agttcttca ttccgtgga gcggcatgt gccaaaaga 
      cccgccccc atttcgcac gcgccgccc acattctat 
      ccaccaacc aaatccccg taactctcg caaaaatcg 
      actgttctg gaaggggtc gcacgacat gtacccaaa 
      aaaacatca cctacccct ttcccgtgt tccacttct 
      accgtaacg gaagcttcg tcaaggagt acaccgggt 
      ctagagatg cccgcagta ttgtgaaat ttatgcgcg 
      ttcaagtgg tgccaaatc cggtcgagt tggtggatt 
      ttgagcact ttcggcagt ttattgaag ggcaggatg 
      atgtaattg ttgtgggtt ttttcgaag aggagacga 
      agctacgga ggatttttt tccgtgtcg cggaggaga 
      tgaaagaga gtatgattt ttgcgtact ctagttgtg 
      agaaggtga ttttgaagc aaggtgtta gtaatggga 
      ttgtggttt ttcggccaa aaagtctcc acaatcagt 
      atgaaccag aacgggtac ttttcacag ggaggagta 
      tcattgggg agataaaaa attttatta cacggaact 
      accacggtt taattggcc acagaacgc ccaacaatc 
      gacacgatt ttcccaacc ctttggtca tcgtctacc 
      acacaatcg actatcact ccggtggta ccgaattaa 
      attactggc gctatcgtt tgattaaaa tcgcccata 
      agtaccaaa acttcgtca aaatcgcca caagtgcta 
      ccgacgatt tcgaagacg aaatttccg acctccgga 
      ccgataacc tcaccccca ctaaccccc tggttgtgg 
      catttgacc tcgagaacc aaaaatacg tcatggtgg 
      aacctttca ccacctcaa cttttgata acttcgtcc 
      agaacttca tcaaccgga aactgactc cctactatc 
      gctccaaac ccatcccca aggaaaacg acggtcctg 
      tgatcattg ccgtcaccc agaacttca accacacgg 
      tgaccaaca acggcaaag acacgcttc tcgatttgt 
      acactccgt ggagtctca aatgccaga agtttgttc 
      cggttttgc gtgaagttg ccgagcttt tggagcacg 
      aagatgtgg cttttgtcc gaatggatg ccgtggaga 
      atcaagtgc ctgaagtgt tcaacgaga aagggatac 
      cgaatattt tctggttgg ccaagaaca ggaaaaggg 
      ggccggtgg tgtacgagg gggagagga gtgccgagg 
      aagttgtca agtttgtgg cgaagaagg cgaccaaag 
      agttgaaga attatgata ggaagggga atcccaagg 
      atgatcgag acgagttgt aattaattt ttgagaata 
      aaaaatttt tttttta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY