Status
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Gene information
General information
TC031384
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011355,TC011354
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 4280839..4287413
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031384
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05019

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    6 analysable objects

    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva cuticle ventral partiallynot closed - Penetrance (percentage of animals/eggs on slide): less than 30%

    labrum not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    antenna not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    Pupal morphology
    wings size shortened - Number of animals showing the phenotype: 4

    wings not closed - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031384 RA
      MARQRRTQG AADPNKGTN SSSSNGSNS TNNRSSKST 
      SLLSYWKQI IGCIVLGIA VSLGYMGYL ETRVNTPFD 
      DKKMVVKSG LQVPEQFWG TYRPGLYFG LKTRDPTSL 
      VTGLMWYFP KRLRPGGDG IRHWCEQGD GLEKYGWVK 
      HDGSNFGVQ EIIDGGYNI DTSFVKRLG GSHGGDWTA 
      RISVTKPGA SEDVSLIWY TALDEETKG FISLTNSAT 
      KFTGIKGET TGLGEFTIK FTNNSGVVS HESFLSTVA 
      PGLHLLKET VVGSLRLAQ DKPNSPRRI VLGGEILPE 
      TGTGKAQPN FVAVQLTGK VPFSIDVVF ESGSFGTRQ 
      DTLVGETYS KALAWHVAR FGQRFEDTF GLHKKGYGP 
      KEVTFAQSA LSNMLGGIG YFYGTSKVQ SFYTKEPVH 
      YWHAPLYTA VPSRSFFPR GFLWDEGFH GFLIATWDL 
      DLELDIISH WFDLMNVEG WIPREQILG VEAIAKIPE 
      EFIVQRNTN ANPPTFFLT LQYILRKYY DKLSEGRLA 
      TLERLYPRL QAWFAWFNT TQKGAGPGM YRWRGRDAT 
      TNRELNPKT LTSGLDDYP RASHPTEAE YHVDLYCWI 
      AIASDTLAR LATLLDRDG YKYEQTADY LKDNFLLDN 
      LHWSNYASR YADYGYHTD SVKLKRPKP LPRSQNQNM 
      EMVRVTLKN PEYRLVDST FGYVSLFPF LLQMIDSDS 
      LKLGQILDD LRNPDLLWT KFGLRSLSK NSPLYMKRN 
      TEHDPPYWR GQIWININF LAVRSLYFY ANTEGPYRD 
      KANAIYKDL RENVIKNVM TQYFKTGYL WEQYNDETG 
      EGSGCYSRL FGACVVNYH LSKSLVMMA SNLAKNFEN 
      VTIDDQEFA EKCHELAQA LESGQVTLL RLVEDLGGL 
      LIDTSPKGR ELGMWVLTR VVETLPPDV LDENQLKFL 
      NQFYTDRLK DNHFVLPAV LRGFLALIR CEGAPKGGP 
      GPFLVQLFH NVSCQQQQE NDRLAIYQI FETCLEKFW 
      PELDEMGPD FVYGVISAV DGERSPKNL IFLFDWFPK 
      FLRKVKLGH LAEDAFEIL ACYFPVDFR APPQDENGI 
      TRNTLAEKL AFCLVAVPQ FAEFSLQLA LEKFDSSLE 
      IAKLDSLRL IELSFVTFD ISPTTVWPL IQKELFQTT 
      NPAIEKACL TTMTAVIKH TRDPNLTET VKDICDTIK 
      GNFLPDSKL FQPSARLII SVANASDSV ANLVARETV 
      PILTNMYNI ANTTAEKTP FLRLLTDLY ALASDLENL 
      GEVPVLLVK SDSPIAFDC LRDISPKLP EKIRLLLYQ 
      NYVKLLQVP QEGAVLQCL EALARDYPN EVATHVVGN 
      TENYTPCFL DALCVTLAN QKFFTAHLI DLILKNCAI 
      KTLQKVLIK FDDCDAIFD VLNAQNITH VLIEMALTG 
      DTCHETIAF VLKIVIGRM SSEKQNEMI RNELSRIKI 
      EDIFLLSGI LTCLRRDVV INYEILSEL IEFIIRERD 
      NLRRNAAIE LVANILNKC PNDDVIREF LERVDTKTD 
      PILTSWLTK ALIMRNHEL GTFWTTKLI DYLDKDESI 
      APCFRTIMS NSESLSTKS HCNVAFLYQ QKFFTIVTN 
      KLSANYSPD KNSYLSGLG YLLEFAPKP AVLVQFRKI 
      SKLIISCLD HTTNHEILI VILRLIRDF ITTKEQVIE 
      ENLGIFLAR FLKLATYDK SMKVRILAL QCLQEVTSK 
      FPIFMLLPH KNDVLKVLG QVIDDKKRI VRREAVEAR 
      SMWFLLDFD K
    CDS sequence
    • >TC031384 RA
      atggcacgt cagagacgg actcagggc gccgcagac 
      cccaataaa ggtacaaac agtagcagc agcaatggt 
      agcaactct accaacaac cgatcttca aaatccact 
      agtctcctt tcatactgg aagcaaatc atcggttgt 
      attgttctg ggaattgcg gtgtctctg ggctacatg 
      gggtacttg gagacacgt gtcaatacc cccttcgac 
      gataagaag atggttgtc aaaagtggg ctacaagtc 
      ccggaacaa ttttggggg acgtacagg ccggggctt 
      tatttcggg ttgaaaaca cgtgacccc acgtcactt 
      gtcaccggt ttaatgtgg tatttcccc aaaaggttg 
      cgtccaggg ggtgatggg atacgtcat tggtgtgaa 
      caaggtgat gggttggaa aaatatggg tgggttaaa 
      catgatggt agtaatttt ggggtacag gaaattatc 
      gatgggggc tataatatc gatacgtcg tttgttaag 
      cgacttggg ggcagccat gggggcgat tggacggct 
      cggatcagt gtcaccaaa cctggggca agtgaggat 
      gtttcactc atctggtat acagcgtta gatgaggaa 
      acgaaaggt tttatctcg cttactaat tctgctacg 
      aaatttacc ggaattaag ggggaaact accggttta 
      ggggaattc actattaag tttacgaat aattccggt 
      gttgtgagt catgaatcg tttttgagt acggtagcc 
      ccgggactt cacctactc aaggaaaca gtggtgggg 
      tcactaaga ctggcacag gacaaacca aattccccc 
      agacgtatt gttttgggc ggggaaata ctcccggaa 
      acgggcacc ggaaaagca caaccaaac ttcgtcgcc 
      gtacaatta acgggtaaa gtcccgttt tcgatcgat 
      gtcgttttc gaatcgggt agtttcggt acaagacag 
      gatacgctg gtgggggag acttacagc aaggcttta 
      gcttggcac gtggccagg tttggacaa aggtttgag 
      gataccttt ggattacac aaaaaaggt tatggtccg 
      aaagaggtg accttcgcc caatcggcc ctcagtaac 
      atgctggga ggcatcggc tatttttac ggcacttcc 
      aaagtgcaa agtttctac accaaagaa ccggtgcac 
      tattggcac gccccctta tacacagca gtcccgtct 
      cgaagtttt ttccctcgt ggttttcta tgggacgaa 
      ggtttccat ggctttctg atcgcaacg tgggacctt 
      gaccttgaa cttgacatt atcagtcat tggttcgat 
      ttgatgaac gttgaaggg tggatcccc cgtgaacaa 
      atcctgggt gttgaagct atagcgaaa atacccgaa 
      gaatttatc gtccaacga aatactaac gcaaatccg 
      ccgactttc ttcctcacg ttgcaatac attttgcgt 
      aaatattac gataagttg agcgaggga cgtttggca 
      acactggaa cggttgtat ccaaggtta caggcgtgg 
      tttgcatgg tttaatacc acgcaaaaa ggggcgggg 
      cctggcatg taccggtgg agggggcgt gacgcaaca 
      accaatcgt gagctgaat ccgaaaact ttaacttcg 
      ggccttgac gattatcca cgagcgtca catccgacc 
      gaagccgaa taccacgtc gatctatac tgttggatc 
      gccatagct agtgatact ttagcaagg ttggcaaca 
      ctgttggac cgtgatggg tataaatac gaacaaact 
      gccgattat ttgaaagac aatttccta ttggataat 
      ttacattgg tctaattac gcatcgcgt tacgccgat 
      tatggttac cacactgat tctgttaaa ttaaaacga 
      ccgaaaccg ttaccaagg tcacaaaac caaaacatg 
      gagatggtg cgtgtgacc ttgaaaaat cccgaatac 
      cgattggtc gactcgact ttcggttac gtcagtttg 
      tttccgttt ttgttacaa atgattgat tcggattcg 
      ttgaaattg ggccagatt ttggatgac cttcgaaat 
      cctgacttg ttatggacg aagtttggt ctaagatca 
      ctgtccaaa aattcaccc ctgtatatg aaacgcaac 
      accgaacat gatccgcca tattggagg ggccaaatt 
      tggataaat atcaatttt ctagcagtc cggtccttg 
      tatttctac gccaatacg gaggggccc tatcgggat 
      aaagccaac gcgatttat aaagatttg cgggaaaat 
      gtgattaaa aacgtgatg acgcaatat ttcaaaacg 
      ggttatctg tgggaacag tataatgac gaaacgggg 
      gaggggagt ggctgttat agtaggtta tttggggca 
      tgcgtggtc aattatcat ctgtcaaaa agtttggtt 
      atgatggct tcgaatttg gccaaaaat ttcgaaaat 
      gttacaata gatgaccaa gaatttgcc gaaaaatgc 
      cacgaattg gcccaagca cttgaatcg ggtcaagtg 
      accttgtta cgccttgtc gaggaccta ggggggctc 
      cttatcgat acgagcccc aaggggcgc gagctgggc 
      atgtgggtg ttgacacgt gtcgtcgag actttgccc 
      cccgatgtt ttggacgaa aatcaatta aaattttta 
      aatcagttt tacacggac cggcttaag gacaatcat 
      tttgtgtta ccggccgtt ttgcgcggt tttctcgcc 
      ttaattcgg tgcgagggg gccccgaaa gggggccct 
      ggccctttt ctcgtccaa ctcttccac aatgtctcg 
      tgccaacaa caacaagaa aacgacaga ttagcaatt 
      tatcaaatt ttcgagact tgtttggag aaattttgg 
      ccagaattg gatgaaatg gggcccgac tttgtctac 
      ggtgttatt tcggcggtt gatggcgaa cgcagcccc 
      aaaaacctg atttttctc tttgattgg tttccgaaa 
      tttttacga aaagtcaaa ttggggcac ctggctgag 
      gacgctttc gaaatcctg gcgtgttat ttccctgtt 
      gattttagg gctccgcca caggacgaa aacggcata 
      actcgaaat accctcgct gaaaagctc gccttttgc 
      ctcgttgcc gttccacaa tttgccgaa ttctccctt 
      caattagcg ttagaaaaa ttcgattct tcgcttgaa 
      attgctaaa ttggattcg ttacgactt attgaacta 
      tcatttgtt acttttgat atttcacca accacagta 
      tggccactc atacaaaaa gaactgttc caaacgaca 
      aatccagca attgaaaaa gcctgttta acaacaatg 
      acagctgtt attaagcac acacgtgac ccaaatttg 
      acagaaacg gtcaaagat atctgcgat acaatcaaa 
      ggaaatttt ttacccgac tcaaaatta ttccaaccg 
      tcggcacgt ttgataatc agtgttgcc aacgcatcg 
      gacagcgtt gccaacctc gtagcgcgt gaaaccgtc 
      cccatatta acaaacatg tataatata gccaacaca 
      acggctgaa aaaacacca tttttgcgc ctactaacc 
      gacttgtat gcacttgcc agcgattta gaaaatctc 
      ggggaagta ccggtttta cttgtaaaa tctgactca 
      ccaatcgct tttgattgt ttacgtgac atttcgccg 
      aaactaccc gaaaaaatc cgactttta ctttaccaa 
      aactacgtc aagttgtta caagtgcca caagaaggc 
      gctgtctta caatgtctt gaagcttta gcacgggat 
      tacccgaac gaagtggct acgcacgtg gtcggtaat 
      acagaaaac tatactcca tgttttcta gacgccttg 
      tgtgtgact ttggcaaat caaaaattt ttcacggcc 
      catttaatc gatttaatt ttgaaaaat tgtgcaata 
      aaaacatta caaaaagtt ttaatcaaa tttgacgat 
      tgtgacgcg atttttgac gttttaaac gcacaaaat 
      atcacccat gtgttgatc gagatggct ttgactggt 
      gatacgtgt cacgaaacg attgcattt gttttgaaa 
      attgtgatt ggtcgaatg tcgagcgaa aaacaaaat 
      gaaatgatt cgaaacgaa ttaagtcga attaagatt 
      gaggatatt tttttacta agcgggatc ttgacttgt 
      ttaaggcgc gatgttgta attaattac gaaatttta 
      agcgaatta attgaattt ataattagg gagagggat 
      aatttgaga cgtaacgca gctatagaa ctggttgca 
      aatattttg aacaaatgt cccaatgat gatgtaatt 
      agggaattt ttggaacgg gttgacact aaaaccgac 
      cccattctt acgtcatgg ctcaccaag gccttaatt 
      atgcgtaat cacgaatta ggcacattc tggaccaca 
      aaattaatc gattatttg gataaagat gaaagtata 
      gctccgtgt tttcgcaca attatgtcc aattcggag 
      tctttaagt accaaaagt cattgcaat gttgcattt 
      ttgtaccag cagaagttt tttacgata gttacgaat 
      aaattaagt gcgaattac agtccagac aagaattcg 
      tatttatca ggccttgga tatttacta gagttcgca 
      ccaaaaccg gccgtttta gtccaattt agaaaaatt 
      tcgaaatta attatttcg tgtcttgac cacaccacc 
      aatcatgag attctcatt gtaattttg cgattaatt 
      cgcgatttt atcacaact aaggaacaa gttattgag 
      gaaaatttg gggattttt ttggcaaga tttctcaaa 
      ttagcaact tatgataaa tctatgaaa gtgcgaatt 
      ttggccctc cagtgttta caagaagtc acttctaag 
      tttccaatt tttatgctt ttgcctcac aaaaatgac 
      gttttaaag gttttagga caagtaatc gatgataag 
      aaacgaatt gtacgaagg gaagcagtg gaggcgcgc 
      tctatgtgg tttttgtta gattttgat aaataa
    mRNA sequence
    • >TC031384 RA
      tgtcaataa aaaatcagc aataaagca cgaaattga 
      cacaaaaaa gcggaacaa tcaatgaaa atatccttc 
      cagtgccgt tttaaaccc cccagtgta gtaaagtcg 
      ggttaaaaa ttgcttttc gggtgccat caaaaccta 
      accctaaaa tggcacgtc agagacgga ctcagggcg 
      ccgcagacc ccaataaag gtacaaaca gtagcagca 
      gcaatggta gcaactcta ccaacaacc gatcttcaa 
      aatccacta gtctccttt catactgga agcaaatca 
      tcggttgta ttgttctgg gaattgcgg tgtctctgg 
      gctacatgg ggtacttgg agacacgtg tcaataccc 
      ccttcgacg ataagaaga tggttgtca aaagtgggc 
      tacaagtcc cggaacaat tttggggga cgtacaggc 
      cggggcttt atttcgggt tgaaaacac gtgacccca 
      cgtcacttg tcaccggtt taatgtggt atttcccca 
      aaaggttgc gtccagggg gtgatggga tacgtcatt 
      ggtgtgaac aaggtgatg ggttggaaa aatatgggt 
      gggttaaac atgatggta gtaattttg gggtacagg 
      aaattatcg atgggggct ataatatcg atacgtcgt 
      ttgttaagc gacttgggg gcagccatg ggggcgatt 
      ggacggctc ggatcagtg tcaccaaac ctggggcaa 
      gtgaggatg tttcactca tctggtata cagcgttag 
      atgaggaaa cgaaaggtt ttatctcgc ttactaatt 
      ctgctacga aatttaccg gaattaagg gggaaacta 
      ccggtttag gggaattca ctattaagt ttacgaata 
      attccggtg ttgtgagtc atgaatcgt ttttgagta 
      cggtagccc cgggacttc acctactca aggaaacag 
      tggtggggt cactaagac tggcacagg acaaaccaa 
      attccccca gacgtattg ttttgggcg gggaaatac 
      tcccggaaa cgggcaccg gaaaagcac aaccaaact 
      tcgtcgccg tacaattaa cgggtaaag tcccgtttt 
      cgatcgatg tcgttttcg aatcgggta gtttcggta 
      caagacagg atacgctgg tgggggaga cttacagca 
      aggctttag cttggcacg tggccaggt ttggacaaa 
      ggtttgagg atacctttg gattacaca aaaaaggtt 
      atggtccga aagaggtga ccttcgccc aatcggccc 
      tcagtaaca tgctgggag gcatcggct atttttacg 
      gcacttcca aagtgcaaa gtttctaca ccaaagaac 
      cggtgcact attggcacg cccccttat acacagcag 
      tcccgtctc gaagttttt tccctcgtg gttttctat 
      gggacgaag gtttccatg gctttctga tcgcaacgt 
      gggaccttg accttgaac ttgacatta tcagtcatt 
      ggttcgatt tgatgaacg ttgaagggt ggatccccc 
      gtgaacaaa tcctgggtg ttgaagcta tagcgaaaa 
      tacccgaag aatttatcg tccaacgaa atactaacg 
      caaatccgc cgactttct tcctcacgt tgcaataca 
      ttttgcgta aatattacg ataagttga gcgagggac 
      gtttggcaa cactggaac ggttgtatc caaggttac 
      aggcgtggt ttgcatggt ttaatacca cgcaaaaag 
      gggcggggc ctggcatgt accggtgga gggggcgtg 
      acgcaacaa ccaatcgtg agctgaatc cgaaaactt 
      taacttcgg gccttgacg attatccac gagcgtcac 
      atccgaccg aagccgaat accacgtcg atctatact 
      gttggatcg ccatagcta gtgatactt tagcaaggt 
      tggcaacac tgttggacc gtgatgggt ataaatacg 
      aacaaactg ccgattatt tgaaagaca atttcctat 
      tggataatt tacattggt ctaattacg catcgcgtt 
      acgccgatt atggttacc acactgatt ctgttaaat 
      taaaacgac cgaaaccgt taccaaggt cacaaaacc 
      aaaacatgg agatggtgc gtgtgacct tgaaaaatc 
      ccgaatacc gattggtcg actcgactt tcggttacg 
      tcagtttgt ttccgtttt tgttacaaa tgattgatt 
      cggattcgt tgaaattgg gccagattt tggatgacc 
      ttcgaaatc ctgacttgt tatggacga agtttggtc 
      taagatcac tgtccaaaa attcacccc tgtatatga 
      aacgcaaca ccgaacatg atccgccat attggaggg 
      gccaaattt ggataaata tcaattttc tagcagtcc 
      ggtccttgt atttctacg ccaatacgg aggggccct 
      atcgggata aagccaacg cgatttata aagatttgc 
      gggaaaatg tgattaaaa acgtgatga cgcaatatt 
      tcaaaacgg gttatctgt gggaacagt ataatgacg 
      aaacggggg aggggagtg gctgttata gtaggttat 
      ttggggcat gcgtggtca attatcatc tgtcaaaaa 
      gtttggtta tgatggctt cgaatttgg ccaaaaatt 
      tcgaaaatg ttacaatag atgaccaag aatttgccg 
      aaaaatgcc acgaattgg cccaagcac ttgaatcgg 
      gtcaagtga ccttgttac gccttgtcg aggacctag 
      gggggctcc ttatcgata cgagcccca aggggcgcg 
      agctgggca tgtgggtgt tgacacgtg tcgtcgaga 
      ctttgcccc ccgatgttt tggacgaaa atcaattaa 
      aatttttaa atcagtttt acacggacc ggcttaagg 
      acaatcatt ttgtgttac cggccgttt tgcgcggtt 
      ttctcgcct taattcggt gcgaggggg ccccgaaag 
      ggggccctg gcccttttc tcgtccaac tcttccaca 
      atgtctcgt gccaacaac aacaagaaa acgacagat 
      tagcaattt atcaaattt tcgagactt gtttggaga 
      aattttggc cagaattgg atgaaatgg ggcccgact 
      ttgtctacg gtgttattt cggcggttg atggcgaac 
      gcagcccca aaaacctga tttttctct ttgattggt 
      ttccgaaat ttttacgaa aagtcaaat tggggcacc 
      tggctgagg acgctttcg aaatcctgg cgtgttatt 
      tccctgttg attttaggg ctccgccac aggacgaaa 
      acggcataa ctcgaaata ccctcgctg aaaagctcg 
      ccttttgcc tcgttgccg ttccacaat ttgccgaat 
      tctcccttc aattagcgt tagaaaaat tcgattctt 
      cgcttgaaa ttgctaaat tggattcgt tacgactta 
      ttgaactat catttgtta cttttgata tttcaccaa 
      ccacagtat ggccactca tacaaaaag aactgttcc 
      aaacgacaa atccagcaa ttgaaaaag cctgtttaa 
      caacaatga cagctgtta ttaagcaca cacgtgacc 
      caaatttga cagaaacgg tcaaagata tctgcgata 
      caatcaaag gaaattttt tacccgact caaaattat 
      tccaaccgt cggcacgtt tgataatca gtgttgcca 
      acgcatcgg acagcgttg ccaacctcg tagcgcgtg 
      aaaccgtcc ccatattaa caaacatgt ataatatag 
      ccaacacaa cggctgaaa aaacaccat ttttgcgcc 
      tactaaccg acttgtatg cacttgcca gcgatttag 
      aaaatctcg gggaagtac cggttttac ttgtaaaat 
      ctgactcac caatcgctt ttgattgtt tacgtgaca 
      tttcgccga aactacccg aaaaaatcc gacttttac 
      tttaccaaa actacgtca agttgttac aagtgccac 
      aagaaggcg ctgtcttac aatgtcttg aagctttag 
      cacgggatt acccgaacg aagtggcta cgcacgtgg 
      tcggtaata cagaaaact atactccat gttttctag 
      acgccttgt gtgtgactt tggcaaatc aaaaatttt 
      tcacggccc atttaatcg atttaattt tgaaaaatt 
      gtgcaataa aaacattac aaaaagttt taatcaaat 
      ttgacgatt gtgacgcga tttttgacg ttttaaacg 
      cacaaaata tcacccatg tgttgatcg agatggctt 
      tgactggtg atacgtgtc acgaaacga ttgcatttg 
      ttttgaaaa ttgtgattg gtcgaatgt cgagcgaaa 
      aacaaaatg aaatgattc gaaacgaat taagtcgaa 
      ttaagattg aggatattt ttttactaa gcgggatct 
      tgacttgtt taaggcgcg atgttgtaa ttaattacg 
      aaattttaa gcgaattaa ttgaattta taattaggg 
      agagggata atttgagac gtaacgcag ctatagaac 
      tggttgcaa atattttga acaaatgtc ccaatgatg 
      atgtaatta gggaatttt tggaacggg ttgacacta 
      aaaccgacc ccattctta cgtcatggc tcaccaagg 
      ccttaatta tgcgtaatc acgaattag gcacattct 
      ggaccacaa aattaatcg attatttgg ataaagatg 
      aaagtatag ctccgtgtt ttcgcacaa ttatgtcca 
      attcggagt ctttaagta ccaaaagtc attgcaatg 
      ttgcatttt tgtaccagc agaagtttt ttacgatag 
      ttacgaata aattaagtg cgaattaca gtccagaca 
      agaattcgt atttatcag gccttggat atttactag 
      agttcgcac caaaaccgg ccgttttag tccaattta 
      gaaaaattt cgaaattaa ttatttcgt gtcttgacc 
      acaccacca atcatgaga ttctcattg taattttgc 
      gattaattc gcgatttta tcacaacta aggaacaag 
      ttattgagg aaaatttgg ggatttttt tggcaagat 
      ttctcaaat tagcaactt atgataaat ctatgaaag 
      tgcgaattt tggccctcc agtgtttac aagaagtca 
      cttctaagt ttccaattt ttatgcttt tgcctcaca 
      aaaatgacg ttttaaagg ttttaggac aagtaatcg 
      atgataaga aacgaattg tacgaaggg aagcagtgg 
      aggcgcgct ctatgtggt ttttgttag attttgata 
      aataaattt tattacatt acaggtttc tgtaattac 
      ccttttcac cattttttt tcctcgaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY