Status
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Gene information
General information
TC031375
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011340,TC011341
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 3949905..3972799
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031375
    iBeetle screen iB_05017

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    iBeetle screen iB_05016

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031375 RA
      MSQIRPMVP RLQHFEDAL QKTGWGLYY KFLVGFASA 
      NLFTNGFVL LCVNFALPL SACDTILTR DDVVSIYVC 
      FNFGKAVGG FLLCSVSDN TGKRCMISK SLIIIFGSC 
      FISAFSHNM YTLYLAVFF LGSGLQANI ASVKIYLAE 
      ILPAERRGF YIVLPDIFW TVGYFSTAA FLYYFGALN 
      SAITEHQGM DMRLTSWRI IFAIGGGLC ITMGCTSAL 
      LEMSPRYLL FKRRIITAT LILKQFYAI NRSKYSETF 
      DVQSQQLVD LISDYNINI DPEPVGLIQ QMNLIIKVM 
      LRMLRIMLQ RPFLKVALV IAVTKLPTL FGLPGTIIY 
      LAQIVDGKT GTVTGGNLI HLSLIFHGL TMCSFTSAI 
      LGYVNNVPL LATCSSFFI IGLTLVEST LFVVVIENF 
      PTAVRSRRK YDKTKLPTP PPDPTEKES EKDDFLAYL 
      DSLQKSNLY SRSTSTFTP IIATEDAFD HLEKLYKLM 
      EQMLELREQ NARLHRRVR DLEHLNNLE KMRKELDVG 
      DDCPELDKD TAFAETILE SILSETKSP KQKISAPSR 
      LRQSISRKG RTRSGSVTV AGLEQEVRG DRRASTCVD 
      SRAKPAKVS KWTKVKAAF KWEKAAPNV SDAKSQDSG 
      IGGMVPVEV ARYLRVPSC DDHGHSPVD SGAAEASTP 
      ASLSTASST EDFHRHDQR SPCEDFRSS DDEHSHNYI 
      PHSKEGSQK SHKSHKTPW AKMKDIIQT RNSFKKKHR 
      LSAHSDDIQ IDVELCSDN EDNVFEDEE KKSPKNYAR 
      MDSNDLPPE VVARYQQAI AEDSDLKKV SKWTRVKKA 
      FLPPPDSVQ DTVEDEIQR NYKQLQKKL SLEFQEKLT 
      EWERMKQSS PGTSNPSSS CGYIEETKD SAFMKKMEE 
      WQKIKSQPS VKRSIQMTS EENLPPEFR KKLQEWEKM 
      RKLSCPKKK LSEVPRWKS LSAPKSETS PTIEYPPLS 
      EDFRKKLEE WKQIKSGAT PEAKSSPKL TRKESSPKH 
      SKKHKDPHD KELQWFEKE LGKIEKEKQ RLERERQKF 
      LEREESLSK LRSSVLGGL KKEVLIHTP SGFYRFEGI 
      SRKFTQKLY EWEKAQGIG PESSTFALL NSSLNRKTS 
      PKHKTPDGT PPLVRSKSA DSIAISALN LTCPLMSHQ 
      PSSLSLNDV EELEKEVLA ESKSSSLQF VEDEPEALI 
      VEVEDIVEE TAAPLPTYS QRIQQPVYQ REELKTMCE 
      GETCAAPKV RRSESAKAQ NNYNLIEEI FSILKNLIE 
      NELEIQRTQ ELMSEQETK ANSDLIRLK MFDESQKAM 
      SGRLNDKIQ RLQEANSSV FASITRDES EMRQVVETL 
      ETVQDLSSE INQLTERLD SELTNRNVY EAFRECGAR 
      SLNPIIETA KDIKTKILE LRRRLSYIC AASDLTAPR 
      RKFSKKLVK KSTSNESNT GSEGRLYVK SDTCNTQSQ 
      GAIKKRIRY RQKSQQNRN LADTDDEED EVQKNTHKK 
      LTRTRSVSE SSGAKKETV VVDESIIQK VVVPTQTNF 
      LDISSLDEE AKPSDSPVT VFVKTTRKL FTPLVESGL 
      SREPDETKD NSSERSSTP VTHLPPVSP APQRKLLKD 
      ISPSIRIMM DKYNQKISE SPATKSGKS SGSASPVAW 
      RSPTAERRV KAQTERYQE ELKKSTPVQ KSASASFLH 
      RQLPTPKTA PSAIAKSSS VATFAKNEH LEAKRLSAE 
      LRLQKLQKA KEEFLNSGP TSAPPQVSE EAIKFPARN 
      RLSQISVDS ESSYDSACG TLIKSASAG MINVDALVY 
      RQIDPEVHG GGYVSLPRS TKKQKGLLG GKFGFSNIA 
      SKFRKVKMR KGKEKMNAV STLCRQSLV VDIQPQLAE 
      GKGGEEGKE TSPGTSRSN SWIKKPKFF KK
    CDS sequence
    • >TC031375 RA
      atgtctcaa atccgccct atggtcccg cgcttgcag 
      cacttcgaa gacgctttg cagaagaca ggatggggg 
      ctttattat aaatttcta gtcggattt gcttcggcg 
      aatttattc accaacggc tttgtgctc ttgtgtgtg 
      aactttgca ctgccttta agtgcctgt gacacgatt 
      ttaacgcga gatgatgtc gtttctatt tatgtgtgc 
      tttaatttt ggaaaagcc gtgggcggt tttctccta 
      tgcagcgtt tccgacaac accggcaaa agatgcatg 
      atttcaaaa agcctgatc attattttt ggaagctgc 
      ttcatttct gcattctct cacaatatg tacaccctg 
      tatctggca gtctttttc ttaggttct ggactacaa 
      gcaaacatt gcttcggta aaaatttac ttggctgag 
      attttgcca gctgagaga agaggtttt tacatcgtt 
      ctgccggat attttttgg acagttggt tatttctca 
      acggctgca tttctttat tattttggg gccttgaat 
      tcagccatc accgaacac cagggtatg gatatgaga 
      cttacttct tggaggatt atttttgcg atcgggggc 
      ggattgtgt attactatg ggctgtact tcggccctt 
      ttggaaatg agtccgcga tatttgttg tttaaaagg 
      aggattatt acagcgacg ctgatttta aagcagttt 
      tatgcaatc aataggtcc aagtatagc gaaactttt 
      gatgtccag tcgcagcaa ctggtggac ttgatatca 
      gactacaac atcaacatt gacccagaa cccgtcggc 
      ctgatccaa caaatgaat cttataatc aaagtaatg 
      ctccgaatg ttgagaatc atgctacaa agacccttc 
      cttaaagta gccctagtc atagctgta actaaatta 
      ccaacttta tttggattg cctggcaca attatttat 
      ctagcccag atcgtagat ggaaaaact ggaacagtc 
      actggtgga aatttaatt catctcagt ctgattttc 
      catggactg acgatgtgt tcttttacg agtgctatc 
      ttggggtac gttaacaac gtcccttta ctggccaca 
      tgttcgtct tttttcatc attggactg actttggtg 
      gagtccaca cttttcgtg gtcgtaatc gaaaatttt 
      ccaactgct gtcaggtct cgtcgaaag tacgacaag 
      acgaagctg cccacgccc ccgcccgac cccaccgag 
      aaagaatcg gaaaaagat gacttcctg gcgtacctc 
      gacagcctc cagaaatcc aacttatac tcccgctcc 
      acgtccacc ttcaccccc atcatcgcc accgaggac 
      gccttcgac cacctggag aagctctac aagctcatg 
      gagcaaatg ctcgaactc cgcgagcag aacgcccgc 
      ctccaccgc cgcgtccgc gacctggag cacctcaac 
      aacttggag aagatgcgc aaggagctg gacgtgggc 
      gacgactgc cccgagctg gacaaggac accgccttc 
      gccgagacc atcctggag tcgatcctc tcggagacg 
      aagagcccc aagcagaag atctccgcg ccttcgagg 
      ctgcgacag tcgatctcg cggaaaggg cgcaccagg 
      agcggctcg gtgacggtg gcgggcctg gagcaggaa 
      gtgaggggc gacaggagg gcgtcgacg tgtgtggac 
      agtagggcc aagccggcc aaagtgtcg aaatggacg 
      aaagtgaag gcggcgttt aagtgggag aaagccgcc 
      cccaatgtg agtgacgcc aagtcgcag gacagtggg 
      attgggggc atggtgccg gtggaggtg gcgaggtat 
      ttgagggtg ccgtcgtgc gacgaccac gggcacagt 
      ccggtggac tcgggggcg gcggaggcg agcacgccg 
      gcgagcctc tccacggct tcgtccacg gaggatttt 
      cacagacat gatcagcga agtccgtgc gaagatttt 
      cgaagttca gatgatgag cattcacat aattacatt 
      ccacattcc aaggaagga tcccaaaaa tcacacaaa 
      agccacaaa acaccatgg gccaagatg aaagatatt 
      atccagacg aggaacagc ttcaaaaag aagcacagg 
      ttgagtgca cacagcgat gacatacaa attgacgtg 
      gagctgtgt agtgacaac gaagacaat gttttcgaa 
      gatgaagag aaaaagtcg cccaaaaac tacgcaaga 
      atggactca aacgacctc ccacctgaa gtggtggcc 
      aggtaccaa caagcaata gctgaggat tctgatttg 
      aagaaagtt tcaaagtgg acgagagtc aagaaggcg 
      tttttgcca cctcctgac agtgtccaa gacaccgtt 
      gaagacgag attcaaaga aattacaag caactgcag 
      aagaaactc agcctggaa ttccaggag aagctcaca 
      gagtgggaa agaatgaag caaagctct cccggaacg 
      tccaaccct tcgtcctct tgtggctac atcgaggaa 
      accaaagac tcagccttt atgaaaaaa atggaagag 
      tggcaaaaa atcaagtcc caaccgtcc gtcaaaaga 
      agcatccag atgaccagt gaggaaaac ctcccacct 
      gagttccgc aaaaagcta caagagtgg gaaaaaatg 
      cggaaactt tcatgcccc aaaaaaaaa ctatccgaa 
      gtaccgcgc tggaagtcg ctcagcgcc cccaaaagt 
      gaaacctcc ccaaccatc gaatacccc cccttatcc 
      gaagatttt agaaagaag ctagaagag tggaaacaa 
      atcaaatcc ggagccaca ccggaagcc aagtcaagc 
      ccgaaacta acccggaaa gaatcatcc cccaaacac 
      agcaaaaag cacaaagac ccccacgac aaagaactc 
      caatggttt gaaaaagag ttgggaaaa atcgaaaaa 
      gaaaaacaa cgactggag cgcgaaaga caaaaattc 
      ctcgagcgg gaggagagt ttatcaaaa ttgcgaagc 
      tcagttctt ggtggtttg aagaaggag gtcctgatc 
      cacacccct tcggggttt taccgcttc gaggggatt 
      tcccggaag tttacccag aaattgtac gagtgggag 
      aaggcgcaa ggcattggt ccagaatcg tccactttt 
      gctcttttg aactcgtct ctgaatcgc aaaactagc 
      cccaaacac aaaactcca gacgggact ccgcctctg 
      gtgcggtcc aagtctgcc gatagtatt gccatttcg 
      gcgctgaat ctcacttgt cctttgatg agccaccaa 
      ccttcgagt ttgtcgctg aacgatgtg gaggagctg 
      gagaaggaa gtcctggct gagtctaaa tcttcgtcg 
      ctgcagttt gttgaagat gagccggag gcgctgatt 
      gttgaagtt gaagatata gttgaggag acggcggcg 
      ccgttgccg acttactca cagaggatt caacaaccg 
      gtgtatcag agggaagaa ttgaagacc atgtgtgag 
      ggtgaaacg tgcgccgcc cccaaagtg cgccgcagc 
      gagtcagcc aaggcccaa aacaattac aatttaatc 
      gaagaaatt ttcagcatt ttgaagaat ctcattgag 
      aacgagtta gagatccag agaactcag gaattgatg 
      agcgagcaa gagacgaaa gccaattcg gatttgatt 
      cggctaaag atgtttgat gagtcgcaa aaagctatg 
      tcaggtcgt ctcaacgac aagatccag cgtttgcaa 
      gaggcgaat tcgagcgtt tttgcgagc attacgaga 
      gatgagtcg gaaatgaga caagttgtt gagacttta 
      gaaaccgtc caagaccta tcgtcggag atcaatcaa 
      ttgaccgaa cgattggat tctgaactg accaatcgt 
      aacgtttac gaagcgttc cgagaatgt ggcgctcgg 
      tctttaaac ccaatcata gaaactgcc aaagacatc 
      aagaccaaa attttggag ttgagaaga cgtctcagt 
      tacatttgt gctgcttca gatctaaca gcccccaga 
      agaaaattt tcaaaaaaa ttggtaaaa aaatcaaca 
      tcaaacgag tccaataca ggaagcgaa ggccgtctg 
      tacgtaaaa tctgacact tgcaacact cagagtcaa 
      ggggctatc aaaaaacgg atccgctac aggcaaaaa 
      agccaacag aaccgtaat ctagcagac actgatgat 
      gaagaagac gaagtgcaa aaaaacacc cacaaaaaa 
      ctaacccgg acaagatct gtttccgaa agtagtgga 
      gcgaagaaa gaaactgtg gtagttgac gaaagcata 
      atccaaaaa gttgtagtt ccgacacaa accaacttc 
      ttggacatt tcttctctt gacgaagaa gcaaaacca 
      tcagattcc cctgttact gttttcgta aaaacaacc 
      agaaaattg ttcactcct ttggtggag agtggacta 
      agtcgggaa ccagacgaa acaaaagac aattcaagc 
      gaacgcagt tccaccccc gtgacccac ctgcctcca 
      gtgtccccc gccccccaa cggaaactc ctcaaggac 
      atttccccc agcatcaga atcatgatg gacaagtac 
      aaccagaaa atctccgaa tcaccagcc accaaatcg 
      gggaaaagc agcggttcc gcttcgcca gtagcctgg 
      cgttcgcca acggcggaa cgaagagtc aaagcccaa 
      acggaacgc taccaagaa gaattaaaa aaatcaacc 
      ccagtgcaa aaatccgca agtgccagt ttcctccac 
      cgccaactc ccaaccccc aaaaccgcc ccctccgcc 
      atcgccaaa tcctcaagt gtcgccact ttcgccaaa 
      aacgaacac ctcgaggcg aagcgactt tccgccgaa 
      ctaagactc caaaaattg caaaaagcc aaggaagaa 
      tttctgaat tctggccca acttctgcc cctccacag 
      gtttcggag gaagcgatc aaatttccc gccagaaac 
      cgcctgagt cagatcagt gtggacagt gagtcgagt 
      tatgattcg gcttgtgga actttgatc aagtcggca 
      agtgctgga atgatcaac gttgatgct ctggtgtac 
      agacaaatt gacccggag gtgcatgga gggggctat 
      gtgtcgctc ccccggagt acgaagaag cagaagggg 
      ctgcttggg gggaagttc gggttttcg aatattgcc 
      tccaagttc aggaaagtg aaaatgagg aaagggaag 
      gagaaaatg aacgcggtt tcgactttg tgtcggcag 
      agcttggtg gtggatatt cagccacag ttggctgag 
      gggaaaggc ggggaggag ggtaaggag acgtcccca 
      gggacttcg aggagcaac agttggata aagaaaccg 
      aaatttttt aagaaatga
    mRNA sequence
    • >TC031375 RA
      gtcaaaaac aacaaatta gctcatttt taatctgcc 
      aacgatcga attgctttc gcctgtttg acaccaaaa 
      accgtcaaa ttttaatta aaactcaaa tgtctcaaa 
      tccgcccta tggtcccgc gcttgcagc acttcgaag 
      acgctttgc agaagacag gatgggggc tttattata 
      aatttctag tcggatttg cttcggcga atttattca 
      ccaacggct ttgtgctct tgtgtgtga actttgcac 
      tgcctttaa gtgcctgtg acacgattt taacgcgag 
      atgatgtcg tttctattt atgtgtgct ttaattttg 
      gaaaagccg tgggcggtt ttctcctat gcagcgttt 
      ccgacaaca ccggcaaaa gatgcatga tttcaaaaa 
      gcctgatca ttatttttg gaagctgct tcatttctg 
      cattctctc acaatatgt acaccctgt atctggcag 
      tctttttct taggttctg gactacaag caaacattg 
      cttcggtaa aaatttact tggctgaga ttttgccag 
      ctgagagaa gaggttttt acatcgttc tgccggata 
      ttttttgga cagttggtt atttctcaa cggctgcat 
      ttctttatt attttgggg ccttgaatt cagccatca 
      ccgaacacc agggtatgg atatgagac ttacttctt 
      ggaggatta tttttgcga tcgggggcg gattgtgta 
      ttactatgg gctgtactt cggcccttt tggaaatga 
      gtccgcgat atttgttgt ttaaaagga ggattatta 
      cagcgacgc tgattttaa agcagtttt atgcaatca 
      ataggtcca agtatagcg aaacttttg atgtccagt 
      cgcagcaac tggtggact tgatatcag actacaaca 
      tcaacattg acccagaac ccgtcggcc tgatccaac 
      aaatgaatc ttataatca aagtaatgc tccgaatgt 
      tgagaatca tgctacaaa gacccttcc ttaaagtag 
      ccctagtca tagctgtaa ctaaattac caactttat 
      ttggattgc ctggcacaa ttatttatc tagcccaga 
      tcgtagatg gaaaaactg gaacagtca ctggtggaa 
      atttaattc atctcagtc tgattttcc atggactga 
      cgatgtgtt cttttacga gtgctatct tggggtacg 
      ttaacaacg tccctttac tggccacat gttcgtctt 
      ttttcatca ttggactga ctttggtgg agtccacac 
      ttttcgtgg tcgtaatcg aaaattttc caactgctg 
      tcaggtctc gtcgaaagt acgacaaga cgaagctgc 
      ccacgcccc cgcccgacc ccaccgaga aagaatcgg 
      aaaaagatg acttcctgg cgtacctcg acagcctcc 
      agaaatcca acttatact cccgctcca cgtccacct 
      tcaccccca tcatcgcca ccgaggacg ccttcgacc 
      acctggaga agctctaca agctcatgg agcaaatgc 
      tcgaactcc gcgagcaga acgcccgcc tccaccgcc 
      gcgtccgcg acctggagc acctcaaca acttggaga 
      agatgcgca aggagctgg acgtgggcg acgactgcc 
      ccgagctgg acaaggaca ccgccttcg ccgagacca 
      tcctggagt cgatcctct cggagacga agagcccca 
      agcagaaga tctccgcgc cttcgaggc tgcgacagt 
      cgatctcgc ggaaagggc gcaccagga gcggctcgg 
      tgacggtgg cgggcctgg agcaggaag tgaggggcg 
      acaggaggg cgtcgacgt gtgtggaca gtagggcca 
      agccggcca aagtgtcga aatggacga aagtgaagg 
      cggcgttta agtgggaga aagccgccc ccaatgtga 
      gtgacgcca agtcgcagg acagtggga ttgggggca 
      tggtgccgg tggaggtgg cgaggtatt tgagggtgc 
      cgtcgtgcg acgaccacg ggcacagtc cggtggact 
      cgggggcgg cggaggcga gcacgccgg cgagcctct 
      ccacggctt cgtccacgg aggattttc acagacatg 
      atcagcgaa gtccgtgcg aagattttc gaagttcag 
      atgatgagc attcacata attacattc cacattcca 
      aggaaggat cccaaaaat cacacaaaa gccacaaaa 
      caccatggg ccaagatga aagatatta tccagacga 
      ggaacagct tcaaaaaga agcacaggt tgagtgcac 
      acagcgatg acatacaaa ttgacgtgg agctgtgta 
      gtgacaacg aagacaatg ttttcgaag atgaagaga 
      aaaagtcgc ccaaaaact acgcaagaa tggactcaa 
      acgacctcc cacctgaag tggtggcca ggtaccaac 
      aagcaatag ctgaggatt ctgatttga agaaagttt 
      caaagtgga cgagagtca agaaggcgt ttttgccac 
      ctcctgaca gtgtccaag acaccgttg aagacgaga 
      ttcaaagaa attacaagc aactgcaga agaaactca 
      gcctggaat tccaggaga agctcacag agtgggaaa 
      gaatgaagc aaagctctc ccggaacgt ccaaccctt 
      cgtcctctt gtggctaca tcgaggaaa ccaaagact 
      cagccttta tgaaaaaaa tggaagagt ggcaaaaaa 
      tcaagtccc aaccgtccg tcaaaagaa gcatccaga 
      tgaccagtg aggaaaacc tcccacctg agttccgca 
      aaaagctac aagagtggg aaaaaatgc ggaaacttt 
      catgcccca aaaaaaaac tatccgaag taccgcgct 
      ggaagtcgc tcagcgccc ccaaaagtg aaacctccc 
      caaccatcg aataccccc ccttatccg aagatttta 
      gaaagaagc tagaagagt ggaaacaaa tcaaatccg 
      gagccacac cggaagcca agtcaagcc cgaaactaa 
      cccggaaag aatcatccc ccaaacaca gcaaaaagc 
      acaaagacc cccacgaca aagaactcc aatggtttg 
      aaaaagagt tgggaaaaa tcgaaaaag aaaaacaac 
      gactggagc gcgaaagac aaaaattcc tcgagcggg 
      aggagagtt tatcaaaat tgcgaagct cagttcttg 
      gtggtttga agaaggagg tcctgatcc acacccctt 
      cggggtttt accgcttcg aggggattt cccggaagt 
      ttacccaga aattgtacg agtgggaga aggcgcaag 
      gcattggtc cagaatcgt ccacttttg ctcttttga 
      actcgtctc tgaatcgca aaactagcc ccaaacaca 
      aaactccag acgggactc cgcctctgg tgcggtcca 
      agtctgccg atagtattg ccatttcgg cgctgaatc 
      tcacttgtc ctttgatga gccaccaac cttcgagtt 
      tgtcgctga acgatgtgg aggagctgg agaaggaag 
      tcctggctg agtctaaat cttcgtcgc tgcagtttg 
      ttgaagatg agccggagg cgctgattg ttgaagttg 
      aagatatag ttgaggaga cggcggcgc cgttgccga 
      cttactcac agaggattc aacaaccgg tgtatcaga 
      gggaagaat tgaagacca tgtgtgagg gtgaaacgt 
      gcgccgccc ccaaagtgc gccgcagcg agtcagcca 
      aggcccaaa acaattaca atttaatcg aagaaattt 
      tcagcattt tgaagaatc tcattgaga acgagttag 
      agatccaga gaactcagg aattgatga gcgagcaag 
      agacgaaag ccaattcgg atttgattc ggctaaaga 
      tgtttgatg agtcgcaaa aagctatgt caggtcgtc 
      tcaacgaca agatccagc gtttgcaag aggcgaatt 
      cgagcgttt ttgcgagca ttacgagag atgagtcgg 
      aaatgagac aagttgttg agactttag aaaccgtcc 
      aagacctat cgtcggaga tcaatcaat tgaccgaac 
      gattggatt ctgaactga ccaatcgta acgtttacg 
      aagcgttcc gagaatgtg gcgctcggt ctttaaacc 
      caatcatag aaactgcca aagacatca agaccaaaa 
      ttttggagt tgagaagac gtctcagtt acatttgtg 
      ctgcttcag atctaacag cccccagaa gaaaatttt 
      caaaaaaat tggtaaaaa aatcaacat caaacgagt 
      ccaatacag gaagcgaag gccgtctgt acgtaaaat 
      ctgacactt gcaacactc agagtcaag gggctatca 
      aaaaacgga tccgctaca ggcaaaaaa gccaacaga 
      accgtaatc tagcagaca ctgatgatg aagaagacg 
      aagtgcaaa aaaacaccc acaaaaaac taacccgga 
      caagatctg tttccgaaa gtagtggag cgaagaaag 
      aaactgtgg tagttgacg aaagcataa tccaaaaag 
      ttgtagttc cgacacaaa ccaacttct tggacattt 
      cttctcttg acgaagaag caaaaccat cagattccc 
      ctgttactg ttttcgtaa aaacaacca gaaaattgt 
      tcactcctt tggtggaga gtggactaa gtcgggaac 
      cagacgaaa caaaagaca attcaagcg aacgcagtt 
      ccacccccg tgacccacc tgcctccag tgtcccccg 
      ccccccaac ggaaactcc tcaaggaca tttccccca 
      gcatcagaa tcatgatgg acaagtaca accagaaaa 
      tctccgaat caccagcca ccaaatcgg ggaaaagca 
      gcggttccg cttcgccag tagcctggc gttcgccaa 
      cggcggaac gaagagtca aagcccaaa cggaacgct 
      accaagaag aattaaaaa aatcaaccc cagtgcaaa 
      aatccgcaa gtgccagtt tcctccacc gccaactcc 
      caaccccca aaaccgccc cctccgcca tcgccaaat 
      cctcaagtg tcgccactt tcgccaaaa acgaacacc 
      tcgaggcga agcgacttt ccgccgaac taagactcc 
      aaaaattgc aaaaagcca aggaagaat ttctgaatt 
      ctggcccaa cttctgccc ctccacagg tttcggagg 
      aagcgatca aatttcccg ccagaaacc gcctgagtc 
      agatcagtg tggacagtg agtcgagtt atgattcgg 
      cttgtggaa ctttgatca agtcggcaa gtgctggaa 
      tgatcaacg ttgatgctc tggtgtaca gacaaattg 
      acccggagg tgcatggag ggggctatg tgtcgctcc 
      cccggagta cgaagaagc agaaggggc tgcttgggg 
      ggaagttcg ggttttcga atattgcct ccaagttca 
      ggaaagtga aaatgagga aagggaagg agaaaatga 
      acgcggttt cgactttgt gtcggcaga gcttggtgg 
      tggatattc agccacagt tggctgagg ggaaaggcg 
      gggaggagg gtaaggaga cgtccccag ggacttcga 
      ggagcaaca gttggataa agaaaccga aatttttta 
      agaaatgat tttgtattt gtactaact gaagcattt 
      atgattaac aattg
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