Status
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Gene information
General information
TC011318 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 3233714..3252748
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011318
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05013

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011318 RA
      MSHLWNDTF NLTGVKINV TIRDDASLY PLGLVIPVT 
      VVYGVIFVT GVIGNVSTC VVISCNKSM HTATNYYLF 
      SLAISDMLL LISGLPPEM YRLWSPELY VFGEVFCIL 
      QGFAAETSA NATVLTITA FTVERYVAI CHPFVSHTM 
      SKLSRAIRH IIVIWVVAL CLAAPQAIQ FGVEYEVKD 
      GVQNSHCTV VSNFFQHAF EISTFVFFV GPMTLITVL 
      YILIGIQLR KPRLAAMKK GSMESSGSN EQARNRNQA 
      AQKRVVNML IAVVVAFFI CWAPFHAQR LMAVYLSTA 
      SKEAQEMTF ELYMVLMHA SGILYFLST TINPVLYHI 
      MSNKFREAF KKTLSDFWR RDGARPSQT YLVLSRCPP 
      LNRQASDSV DSHTKEELH LKSGLSHRP YNFLGCNKI 
      KQDSSTISE CKTLQSTVS SESKPAQRS DKFRILRVL 
      GPQKRPVKF FRQESVDSG HTISNSSLQ DVEEREHSS 
      SDLVKYMEE INEDIT
    CDS sequence
    • >TC011318 RA
      atgagtcat ctctggaac gacactttc aacctgact 
      ggagtcaaa atcaacgtt acaataagg gacgatgcc 
      tcgctgtac cctctgggg ttggtgatt ccggtgacg 
      gtggtgtat ggcgtgatt ttcgtgaca ggggtgatt 
      ggaaatgtg agcacgtgt gttgttatt tcctgtaac 
      aagtcgatg catacggcg accaattat tatctcttc 
      agtttggcg atttctgat atgttgctg ttgatttcg 
      ggacttccc ccggaaatg taccggctg tggtcgccg 
      gaactttac gtttttggg gaagtgttt tgcattctg 
      cagggtttt gcagccgaa acttcggcc aatgcaacg 
      gttttaacc atcacagct tttacagtg gagcgttac 
      gtggcaatt tgtcacccc ttcgtttcg cacacaatg 
      tccaaactt tcgagggcc attagacac attatcgtg 
      atttgggtg gtggctttg tgtcttgca gccccgcaa 
      gcaatccaa ttcggtgtc gaatatgag gtgaaagat 
      ggggttcag aacagccat tgtaccgtc gtatcgaat 
      tttttccaa cacgctttt gaaatttca acgttcgtg 
      ttcttcgtg ggcccaatg acgcttatt acggtcctg 
      tatatactg atcggcatt cagttgagg aaaccgaga 
      ctggctgcc atgaaaaaa gggtcaatg gagagctct 
      ggaagcaac gaacaggcc aggaaccgg aatcaagcg 
      gcccagaag agggtcgta aatatgttg attgcagtg 
      gtggtggcc ttcttcatc tgttgggct cctttccat 
      gcgcagaga ctgatggct gtttatttg tccaccgcg 
      tccaaggag gcgcaggag atgactttc gagctctat 
      atggtgctt atgcacgca tccggaatt ttatacttt 
      ctctctacg accattaat ccggtgctt tatcatatt 
      atgtcgaat aaatttcgc gaggcattc aagaaaact 
      ttgtccgat ttctggcgt cgcgacggc gcccgtcca 
      tcccaaacc tacttggtg ttaagccgc tgtccacca 
      cttaaccgc caagcttca gactcagtc gacagccac 
      accaaagaa gaactccac ctcaaaagc ggtctcagc 
      caccgccct tacaatttc ctcggctgc aacaaaata 
      aagcaagac tcctcgaca ataagcgag tgcaaaacc 
      ctccagtcc acggtctcg agtgaatca aaacccgct 
      caaaggagc gacaaattc cggattttg cgcgtcctc 
      ggcccacag aaacgtcca gtgaagttt tttagacag 
      gagagtgtc gacagcggc cacactatc agcaattcg 
      agcctccag gacgtggag gaaagggaa cacagcagc 
      tcggatttg gtcaaatac atggaagag atcaacgaa 
      gatatcacc tag
    mRNA sequence
    • >TC011318 RA
      tcgcttcag caaaaccgt ctccatcca atttgctaa 
      ttaattcaa taacgagag tcgcaaaaa tgtgaagct 
      cggaatgag tcatctctg gaacgacac tttcaacct 
      gactggagt caaaatcaa cgttacaat aagggacga 
      tgcctcgct gtaccctct ggggttggt gattccggt 
      gacggtggt gtatggcgt gattttcgt gacaggggt 
      gattggaaa tgtgagcac gtgtgttgt tatttcctg 
      taacaagtc gatgcatac ggcgaccaa ttattatct 
      cttcagttt ggcgatttc tgatatgtt gctgttgat 
      ttcgggact tcccccgga aatgtaccg gctgtggtc 
      gccggaact ttacgtttt tggggaagt gttttgcat 
      tctgcaggg ttttgcagc cgaaacttc ggccaatgc 
      aacggtttt aaccatcac agcttttac agtggagcg 
      ttacgtggc aatttgtca ccccttcgt ttcgcacac 
      aatgtccaa actttcgag ggccattag acacattat 
      cgtgatttg ggtggtggc tttgtgtct tgcagcccc 
      gcaagcaat ccaattcgg tgtcgaata tgaggtgaa 
      agatggggt tcagaacag ccattgtac cgtcgtatc 
      gaatttttt ccaacacgc ttttgaaat ttcaacgtt 
      cgtgttctt cgtgggccc aatgacgct tattacggt 
      cctgtatat actgatcgg cattcagtt gaggaaacc 
      gagactggc tgccatgaa aaaagggtc aatggagag 
      ctctggaag caacgaaca ggccaggaa ccggaatca 
      agcggccca gaagagggt cgtaaatat gttgattgc 
      agtggtggt ggccttctt catctgttg ggctccttt 
      ccatgcgca gagactgat ggctgttta tttgtccac 
      cgcgtccaa ggaggcgca ggagatgac tttcgagct 
      ctatatggt gcttatgca cgcatccgg aattttata 
      ctttctctc tacgaccat taatccggt gctttatca 
      tattatgtc gaataaatt tcgcgaggc attcaagaa 
      aactttgtc cgatttctg gcgtcgcga cggcgcccg 
      tccatccca aacctactt ggtgttaag ccgctgtcc 
      accacttaa ccgccaagc ttcagactc agtcgacag 
      ccacaccaa agaagaact ccacctcaa aagcggtct 
      cagccaccg cccttacaa tttcctcgg ctgcaacaa 
      aataaagca agactcctc gacaataag cgagtgcaa 
      aaccctcca gtccacggt ctcgagtga atcaaaacc 
      cgctcaaag gagcgacaa attccggat tttgcgcgt 
      cctcggccc acagaaacg tccagtgaa gttttttag 
      acaggagag tgtcgacag cggccacac tatcagcaa 
      ttcgagcct ccaggacgt ggaggaaag ggaacacag 
      cagctcgga tttggtcaa atacatgga agagatcaa 
      cgaagatat cacctagcg cacagcgaa tcgcttgaa 
      gcgattaga aactattcc ggaactgtc tgatctcag 
      tgtgtatct gtgattcgt atctctgtt tggtgtgta 
      cttaatttt taagtgggc gttgaagcg gttttgatt 
      tgatttgta cgagtatta aaaatattt gggtaaaat 
      ttttaattt gataaaaag ggcaagttt tattaaaat 
      tgtgttttt aggaaattg aatgtttta acgttattt 
      caaattctt aatgtgctg tgacctgtg accataaaa 
      acttgctgg taactgtta atttactgt gaaatttgt 
      tctccggtt tattctgta ccttttcca catatttgt 
      aaaacgaat taaaaatac aattcttat gttcaatgt 
      taactgtgc tgtgactta aaaaaactt gacgtaaaa 
      tgttgattt caatttgaa aataattat tcggtaata 
      aatcagaca tttctgtac ctttttatt tttttaaaa 
      tgtattact gaattgatg gaaaccact gatttaaat 
      gtgagactt gtccttaaa ttataagac tttctgtac 
      ctttcctac atatttgta aaacgaata aataaaaag 
      tctatttgt ta
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