| General information | |||
| TC011305 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007425.3: 3104115..3115809 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC011305 | ||
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05010 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)Days post injection
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Days post injection
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 20
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
Fertility
egg production: number <50% of expected -
tropharium size shortened - Number of animals showing the phenotype: 4
tropharium shape irregular - Number of animals showing the phenotype: 4
fertility after larval injection decreased (no living offspring) -
tropharium size shortened - Number of animals showing the phenotype: 4
tropharium shape irregular - Number of animals showing the phenotype: 4
fertility after larval injection decreased (no living offspring) -
- >TC011305 RA
MGSLQQPNR FRPFNQTTQ STFPQSLSG SETDISTSN ENLSNEEKY VIRHTARQE PQGQENQQA SNRSSLRDS LPSNRSSLD VSSSSYNTL IIHSAGDEP WTGNAPHVT TSPHAAIAG RNSGVRESG DAYHPHQPS NLYDPKLSP NQKRSSASP SPSVRTSFD GNTRIYDTG VQEITEIPD DYLSQSSVL KHLAKEVKV PSPKSDFSF FDNPPPEYP KWADKSPLQ AAVHRNNVN NEKLTLSKS QPDLSKVGV PKGAADFDG FRRGVSAPR PPTKGREEA ETGGGELWP SAEMVEILI KENSALKIE LENCYKRVA KTQKLEQEI LKVHKAQEE LVQSCERKE RLERAARGR LQNDNRRLQ ELNRALREQ VDLLSTQML SRSPAPDSG SPDCLRREL SKREVLIAQ LIQQNKEHV AAKERQEIE LAAQRVTLQ EQRTHIDIL DTALTNAQA NVVRLEEEC RKKQVYVER VVQLQRALS SLQLASDRR EQTERKLRL QLERELQRT KLAQGEGGK SGWDSPNGS GNESPSELK RRLREKEEK IMQLEGDCT KWEQRYLEE SALRQAAID AASIPKDAK IAALEKTSQ ETEKIIAEA RNEKIRHMD EVHAAQKKV ADLESRVKD LESKLAERD AMIKVLQKH TYDKDVSSM LGRSPHHTP HPSLGGADI DHVLGTAVS REELVSSVL TSSTGFGSG NSYTGSDSS YHMPSYTKY ENKSFDSTK QSLDNQLKE IDSQLDSQL LSKRGLCCF PGFTNPVTA QRKGKIPKP LLAGVTGGG AGGGVAMYE PGAMLLLEK QGRASQQQR MQDTADASP RCPAPSSLP RPPRPSHRS GLPRRLGDY NRLAEGETP PRKKSDGAD SLSSNASSR RASPARSLI PPRKLGEYG RLSESGGSS ASLRKPTNQ RSRSGGERD SASTLSDNS ASSLPTPSK VRLFGGGAM RRGGSLGRD SKSSTDSTT SKYRIQF
- >TC011305 RB
MGSLQQPNR FRPFNQTTQ STFPQSLSG SETDISTSN ENLSNEEKY VIRHTARQE PQGQENQQA SNRSSLRDS LPSNRSSLD VSSSSYNTL IIHSAGDEP WTGRNSGVR ESGDAYHPH QPSNLYDPK LSPNQKRSS ASPSPSVRT SFDGNTRIY DTGVQEITE IPDDYLSQS SVLKHLAKE VKVPSPKSD FSFFDNPPP EYPKWADKS PLQAAVHRN NVNNEKLTL SKSQPDLSK VGVPKGAAD FDGFRRGVS APRPPTKGR EEAETGGGE LWPSAEMVE ILIKENSAL KIELENCYK RVAKTQKLE QEILKVHKA QEELVQSCE RKERLERAA RGRLQNDNR RLQELNRAL REQVDLLST QMLSRSPAP DSGSPDCLR RELSKREVL IAQLIQQNK EHVAAKERQ EIELAAQRV TLQEQRTHI DILDTALTN AQANVVRLE EECRKKQVY VERVVQLQR ALSSLQLAS DRREQTERK LRLQLEREL QRTKLAQGE GGKSGWDSP NGSGNESPS ELKRRLREK EEKIMQLEG DCTKWEQRY LEESALRQA AIDAASIPK DAKIAALEK TSQETEKII AEARNEKIR HMDEVHAAQ KKVADLESR VKDLESKLA ERDAMIKVL QKHTYDKDV SSMLGRSPH HTPHPSLGG ADIDHVLGT AVSREELVS SVLTSSTGF GSGNSYTGS DSSYHMPSY TKYENKSFD STKQSLDNQ LKEIDSQLD SQLLSKRGL CCFPGFTNP VTAQRKGKI PKPLLAGVT GGGAGGGVA MYEPGAMLL LEKQGRASQ QQRMQDTAD ASPRCPAPS SLPRPPRPS HRSGLPRRL GDYNRLAEG ETPPRKKSD GADSLSSNA SSRRASPAR SLIPPRKLG EYGRLSESG GSSASLRKP TNQRSRSGG ERDSASTLS DNSASSLPT PSKVRLFGG GAMRRGGSL GRDSKSSTD STTSKYRIQ F
- >TC011305 RA
atgggttcg ttgcaacaa cccaaccgc ttccgccct ttcaaccag accactcag agcaccttc cctcaaagc ctgagtggc agcgagact gacatttcc acctcaaat gaaaacctc tcgaacgaa gaaaagtac gtgatacgt cacaccgcc cgccaggag ccccaaggc caggagaac cagcaagcc tcaaatcgc agctccctg cgagacagc ctcccctcg aaccgcagc tcgctggac gtctcctcc tcctcgtac aacaccctc atcatccac agcgcgggg gacgagccc tggacaggt aacgccccc cacgtgacc acctcccct cacgccgcc attgcagga cgcaactcc ggtgtgagg gaatcgggc gacgcctac cacccccac caaccctcg aatctgtac gaccccaaa ctctcgccc aatcagaag cgaagctcc gcctcgccc tcgccctcc gtccggacg agctttgac ggaaacact cgaatctac gacaccggg gtccaggaa atcaccgaa atccccgat gactatctc agccaatcc tcagtcctc aaacacttg gccaaagaa gtcaaggtc ccgtccccc aaatccgac ttttccttc ttcgataac ccgccccct gagtaccca aagtgggcc gataagtcg ccgttacag gcggctgtg catcgaaat aacgtcaat aacgagaaa ttgacactc tcgaagtcg caacccgac ctgtccaag gtgggcgtg cccaaaggg gcggccgac ttcgatggg ttcaggcgg ggcgtgtcc gcgccgaga ccgcccacc aaaggcagg gaggaggcg gagacaggg ggcggggag ttgtggccc tcagccgaa atggtcgaa atactcatc aaggagaac agtgccttg aaaatcgaa ttggagaat tgctacaaa agagttgca aagacacaa aagcttgaa caagaaatt ctaaaagta cacaaggca caggaagag ttagttcag tcgtgcgag cgcaaagaa cggctcgaa cgagccgct agggggcgt ttgcaaaat gacaatcgc cggttgcaa gagttgaat cgggccctg cgtgagcaa gttgatttg ttatcaact caaatgttg tcaaggtca ccggcgccc gattcaggc tcacccgat tgtttgcgg agagagttg agcaagagg gaggtgttg atcgctcag ttgattcag cagaataag gaacatgtt gctgcgaaa gaacgccaa gaaatcgag ttggccgcg caaagagtt acacttcag gaacaacgc acccatatc gatattcta gacaccgca cttaccaac gctcaagcg aatgtcgtc cgtttggag gaagaatgc cgcaaaaag caagtgtac gtggagcgc gtggtgcag ctacagagg gcgctgtcg tcgctccag cttgcgagc gaccgtcgc gagcaaacc gagcgcaaa ttgcgtttg cagctcgag cgcgagctc cagcgcacc aaactggcg caaggcgag ggcggtaaa agtggctgg gattccccg aatggttcg ggtaacgaa tcaccgtcc gaattaaaa cgacgcctg cgcgaaaaa gaggagaaa atcatgcaa ttggagggc gattgcacc aagtgggag cagaggtat ttggaggag agtgcgttg agacaggcg gccatcgac gcggcaagc attcccaag gatgcgaaa attgccgcc ctggagaag accagtcaa gagacggag aaaatcatc gccgaggcg agaaacgag aaaattaga catatggac gaagtccat gccgcccaa aagaaagtt gccgatttg gagtcgcgt gttaaagat ctcgaatcg aagttggct gagcgcgac gctatgatt aaagtattg cagaagcat acgtacgat aaggacgta tcgagtatg ctggggcgg tccccccac catacgccc caccccagt ctgggcggg gccgacatt gatcacgtg ttgggaacg gcggtgtcg agggaggag ttagtcagt agcgtccta acgagttct accggtttt ggatcgggg aattcctac accgggagt gattcgtcc taccatatg ccttcttat accaaatat gaaaataaa agttttgat tcgacgaaa caaagtttg gataatcag ctaaaagaa atcgatagt caactcgat tcgcaactg ttgagcaag cggggtctt tgctgtttt ccaggattc actaatcca gtcactgcg cagagaaaa ggaaaaata cccaagcca ctattggcg ggtgtgacc ggcggcggc gctgggggc ggagtcgcg atgtacgag cccggcgcg atgctcctg ttggagaag cagggccgc gcctcccag cagcaacgc atgcaggac actgccgac gcctcgccg cgctgcccc gcccccagc tcgctgccg cgtcccccg cgcccctcc caccgcagc ggcctgccg cgccgcctc ggcgactac aataggcta gccgagggc gagactccg ccccggaag aagtccgac ggcgccgat tcgctcagt tccaacgcc agcagccgg agggcgtcg ccggcgcgc tcgctgatc ccgccccgg aagctgggc gaatacggg aggctgtca gagagtggc ggctcgagc gcctccctc cgcaaaccg accaatcag agatcgagg agtggcggc gagagagac tccgcctcc acgctcagc gataactcg gccagttcc ttgccaacg ccctccaaa gtgaggctc ttcgggggc ggggcgatg agaagaggc ggctctttg gggcgggac agcaaaagc tccaccgat tcgacaacg agcaagtat cgtatacag ttttag
- >TC011305 RB
atgggttcg ttgcaacaa cccaaccgc ttccgccct ttcaaccag accactcag agcaccttc cctcaaagc ctgagtggc agcgagact gacatttcc acctcaaat gaaaacctc tcgaacgaa gaaaagtac gtgatacgt cacaccgcc cgccaggag ccccaaggc caggagaac cagcaagcc tcaaatcgc agctccctg cgagacagc ctcccctcg aaccgcagc tcgctggac gtctcctcc tcctcgtac aacaccctc atcatccac agcgcgggg gacgagccc tggacagga cgcaactcc ggtgtgagg gaatcgggc gacgcctac cacccccac caaccctcg aatctgtac gaccccaaa ctctcgccc aatcagaag cgaagctcc gcctcgccc tcgccctcc gtccggacg agctttgac ggaaacact cgaatctac gacaccggg gtccaggaa atcaccgaa atccccgat gactatctc agccaatcc tcagtcctc aaacacttg gccaaagaa gtcaaggtc ccgtccccc aaatccgac ttttccttc ttcgataac ccgccccct gagtaccca aagtgggcc gataagtcg ccgttacag gcggctgtg catcgaaat aacgtcaat aacgagaaa ttgacactc tcgaagtcg caacccgac ctgtccaag gtgggcgtg cccaaaggg gcggccgac ttcgatggg ttcaggcgg ggcgtgtcc gcgccgaga ccgcccacc aaaggcagg gaggaggcg gagacaggg ggcggggag ttgtggccc tcagccgaa atggtcgaa atactcatc aaggagaac agtgccttg aaaatcgaa ttggagaat tgctacaaa agagttgca aagacacaa aagcttgaa caagaaatt ctaaaagta cacaaggca caggaagag ttagttcag tcgtgcgag cgcaaagaa cggctcgaa cgagccgct agggggcgt ttgcaaaat gacaatcgc cggttgcaa gagttgaat cgggccctg cgtgagcaa gttgatttg ttatcaact caaatgttg tcaaggtca ccggcgccc gattcaggc tcacccgat tgtttgcgg agagagttg agcaagagg gaggtgttg atcgctcag ttgattcag cagaataag gaacatgtt gctgcgaaa gaacgccaa gaaatcgag ttggccgcg caaagagtt acacttcag gaacaacgc acccatatc gatattcta gacaccgca cttaccaac gctcaagcg aatgtcgtc cgtttggag gaagaatgc cgcaaaaag caagtgtac gtggagcgc gtggtgcag ctacagagg gcgctgtcg tcgctccag cttgcgagc gaccgtcgc gagcaaacc gagcgcaaa ttgcgtttg cagctcgag cgcgagctc cagcgcacc aaactggcg caaggcgag ggcggtaaa agtggctgg gattccccg aatggttcg ggtaacgaa tcaccgtcc gaattaaaa cgacgcctg cgcgaaaaa gaggagaaa atcatgcaa ttggagggc gattgcacc aagtgggag cagaggtat ttggaggag agtgcgttg agacaggcg gccatcgac gcggcaagc attcccaag gatgcgaaa attgccgcc ctggagaag accagtcaa gagacggag aaaatcatc gccgaggcg agaaacgag aaaattaga catatggac gaagtccat gccgcccaa aagaaagtt gccgatttg gagtcgcgt gttaaagat ctcgaatcg aagttggct gagcgcgac gctatgatt aaagtattg cagaagcat acgtacgat aaggacgta tcgagtatg ctggggcgg tccccccac catacgccc caccccagt ctgggcggg gccgacatt gatcacgtg ttgggaacg gcggtgtcg agggaggag ttagtcagt agcgtccta acgagttct accggtttt ggatcgggg aattcctac accgggagt gattcgtcc taccatatg ccttcttat accaaatat gaaaataaa agttttgat tcgacgaaa caaagtttg gataatcag ctaaaagaa atcgatagt caactcgat tcgcaactg ttgagcaag cggggtctt tgctgtttt ccaggattc actaatcca gtcactgcg cagagaaaa ggaaaaata cccaagcca ctattggcg ggtgtgacc ggcggcggc gctgggggc ggagtcgcg atgtacgag cccggcgcg atgctcctg ttggagaag cagggccgc gcctcccag cagcaacgc atgcaggac actgccgac gcctcgccg cgctgcccc gcccccagc tcgctgccg cgtcccccg cgcccctcc caccgcagc ggcctgccg cgccgcctc ggcgactac aataggcta gccgagggc gagactccg ccccggaag aagtccgac ggcgccgat tcgctcagt tccaacgcc agcagccgg agggcgtcg ccggcgcgc tcgctgatc ccgccccgg aagctgggc gaatacggg aggctgtca gagagtggc ggctcgagc gcctccctc cgcaaaccg accaatcag agatcgagg agtggcggc gagagagac tccgcctcc acgctcagc gataactcg gccagttcc ttgccaacg ccctccaaa gtgaggctc ttcgggggc ggggcgatg agaagaggc ggctctttg gggcgggac agcaaaagc tccaccgat tcgacaacg agcaagtat cgtatacag ttttag
- >TC011305 RA
ttcgccacc taaaattgt taaattttg agtttttgg cttaatctc gagtaaaac tgccgtggt tttgtttcc cgttttgtt tctaaaatt atcagaaat ttcgattgg ctggcgtta ttttttcta acggtgagc ctaacattt gtggtaaag tttgtatat tacaatgga atgtgaaac tcgcgtgga atttttggt gctccccct aacggaaaa tacagcagt tgaggtttg ctgaagttt gtaacgttt agtgcctct tggtcacct tttcttcgt tttagaagt gtaaaaaat gcccaaaat ctgactcag atcgtcccc caagtgcct gttccagct tctatgttc agtcataaa agttttgga tcacataaa ccgctctaa atgggttcg ttgcaacaa cccaaccgc ttccgccct ttcaaccag accactcag agcaccttc cctcaaagc ctgagtggc agcgagact gacatttcc acctcaaat gaaaacctc tcgaacgaa gaaaagtac gtgatacgt cacaccgcc cgccaggag ccccaaggc caggagaac cagcaagcc tcaaatcgc agctccctg cgagacagc ctcccctcg aaccgcagc tcgctggac gtctcctcc tcctcgtac aacaccctc atcatccac agcgcgggg gacgagccc tggacaggt aacgccccc cacgtgacc acctcccct cacgccgcc attgcagga cgcaactcc ggtgtgagg gaatcgggc gacgcctac cacccccac caaccctcg aatctgtac gaccccaaa ctctcgccc aatcagaag cgaagctcc gcctcgccc tcgccctcc gtccggacg agctttgac ggaaacact cgaatctac gacaccggg gtccaggaa atcaccgaa atccccgat gactatctc agccaatcc tcagtcctc aaacacttg gccaaagaa gtcaaggtc ccgtccccc aaatccgac ttttccttc ttcgataac ccgccccct gagtaccca aagtgggcc gataagtcg ccgttacag gcggctgtg catcgaaat aacgtcaat aacgagaaa ttgacactc tcgaagtcg caacccgac ctgtccaag gtgggcgtg cccaaaggg gcggccgac ttcgatggg ttcaggcgg ggcgtgtcc gcgccgaga ccgcccacc aaaggcagg gaggaggcg gagacaggg ggcggggag ttgtggccc tcagccgaa atggtcgaa atactcatc aaggagaac agtgccttg aaaatcgaa ttggagaat tgctacaaa agagttgca aagacacaa aagcttgaa caagaaatt ctaaaagta cacaaggca caggaagag ttagttcag tcgtgcgag cgcaaagaa cggctcgaa cgagccgct agggggcgt ttgcaaaat gacaatcgc cggttgcaa gagttgaat cgggccctg cgtgagcaa gttgatttg ttatcaact caaatgttg tcaaggtca ccggcgccc gattcaggc tcacccgat tgtttgcgg agagagttg agcaagagg gaggtgttg atcgctcag ttgattcag cagaataag gaacatgtt gctgcgaaa gaacgccaa gaaatcgag ttggccgcg caaagagtt acacttcag gaacaacgc acccatatc gatattcta gacaccgca cttaccaac gctcaagcg aatgtcgtc cgtttggag gaagaatgc cgcaaaaag caagtgtac gtggagcgc gtggtgcag ctacagagg gcgctgtcg tcgctccag cttgcgagc gaccgtcgc gagcaaacc gagcgcaaa ttgcgtttg cagctcgag cgcgagctc cagcgcacc aaactggcg caaggcgag ggcggtaaa agtggctgg gattccccg aatggttcg ggtaacgaa tcaccgtcc gaattaaaa cgacgcctg cgcgaaaaa gaggagaaa atcatgcaa ttggagggc gattgcacc aagtgggag cagaggtat ttggaggag agtgcgttg agacaggcg gccatcgac gcggcaagc attcccaag gatgcgaaa attgccgcc ctggagaag accagtcaa gagacggag aaaatcatc gccgaggcg agaaacgag aaaattaga catatggac gaagtccat gccgcccaa aagaaagtt gccgatttg gagtcgcgt gttaaagat ctcgaatcg aagttggct gagcgcgac gctatgatt aaagtattg cagaagcat acgtacgat aaggacgta tcgagtatg ctggggcgg tccccccac catacgccc caccccagt ctgggcggg gccgacatt gatcacgtg ttgggaacg gcggtgtcg agggaggag ttagtcagt agcgtccta acgagttct accggtttt ggatcgggg aattcctac accgggagt gattcgtcc taccatatg ccttcttat accaaatat gaaaataaa agttttgat tcgacgaaa caaagtttg gataatcag ctaaaagaa atcgatagt caactcgat tcgcaactg ttgagcaag cggggtctt tgctgtttt ccaggattc actaatcca gtcactgcg cagagaaaa ggaaaaata cccaagcca ctattggcg ggtgtgacc ggcggcggc gctgggggc ggagtcgcg atgtacgag cccggcgcg atgctcctg ttggagaag cagggccgc gcctcccag cagcaacgc atgcaggac actgccgac gcctcgccg cgctgcccc gcccccagc tcgctgccg cgtcccccg cgcccctcc caccgcagc ggcctgccg cgccgcctc ggcgactac aataggcta gccgagggc gagactccg ccccggaag aagtccgac ggcgccgat tcgctcagt tccaacgcc agcagccgg agggcgtcg ccggcgcgc tcgctgatc ccgccccgg aagctgggc gaatacggg aggctgtca gagagtggc ggctcgagc gcctccctc cgcaaaccg accaatcag agatcgagg agtggcggc gagagagac tccgcctcc acgctcagc gataactcg gccagttcc ttgccaacg ccctccaaa gtgaggctc ttcgggggc ggggcgatg agaagaggc ggctctttg gggcgggac agcaaaagc tccaccgat tcgacaacg agcaagtat cgtatacag ttttaggtt agaagcggc ttccgctat catgtgcct gatttaaat attttggac tttttgtcc gaattgtag ctagttagg ttgcctcga tgttatttt gtctttacc gggtttctt ttgttttta cgcttgtta ttattgtat tgtagaaca gtctatagt cagtcttta cttactaac tagtcttat taaaccaaa tagatcaaa gctagggac caaattttt tgttccagt ggcagattg ttgtttctt tattatttt cttttttat gttaggtaa agacaaata aggccatat cctacaaag tgccttcag tagataaac aaggtctct cgagaaagt gtactagtg gtttaattg aaattttct gggcagtat ttttcaata aatttcata ttatcagtt gaaattttt aaagtgcat tgattattt ggcataaat caaatgttc cgtccgttt agtaaaata caggctgtt ccgtctaaa ccgcgcatt actgaaaat gttaatagt gattgtgca ggagctgcg ggattatgg ttgtataca acttccaat acttctaaa atagggaca ccctgtata atattccgg aaaattcgt attgggcca ctctatgga ttcgaatct cctatttac actttttta tacaaagtt ttcttgttt ctagtaata cagtctagt ctgtttata caaaaaatg tattcgaaa tgtacagac aataaatca gttttacca ac
- >TC011305 RB
tttgtataa tatgataac aatggtttg ttgtatttt catgtaaat aaagatttt ttggtgttg tttttattt ttttattaa tgttttgct acaaacagt agccaacgc agaagctat acgaagaaa tactaatta ctgatagca aaatagcaa gggagcttt cgccaccta aaattgtta aattttgag tttttggct taatctcga gtaaaactg ccgtggttt tgtttcccg ttttgtttc taaaattat cagaaattt cgattggct ggcgttatt ttttctaac ggtgagcct aacatttgt ggtaaagtt tgtatatta caatggaat gtgaaactc gcgtggaat ttttggtgc tccccctaa cggaaaata cagcagttg aggtttgct gaagtttgt aacgtttag tgcctcttg gtcaccttt tcttcgttt tagaagtgt aaaaaatgc ccaaaatct gactcagat cgtccccca agtgcctgt tccagcttc tgtaagtat cagcacgtg ctgtgcccc attttcggc gtgaaaagt cgcaaaatt tcgtgcgtt tcttgtgaa agttttgtg tttattctt tgcctggca cattccgat ctaattttc tctcttctc cacaccttt tcaatggtc acatttgaa atcccgtgc catagcgtg gatttgtgt aaagtgatg acggccgaa ttcctgaaa tattttttg cattagagc agccaataa tgtgcaatt aaaagtaaa cattgttaa cctcttgac cagatcaag tgatacggc caaatatcg taacaattt cgccgtttt tctgcgtca attctttgt tttcgtggt taaaattcc ccattttcg tcagatgtt cagtcataa aagttttgg atcacataa accgctcta aatgggttc gttgcaaca acccaaccg cttccgccc tttcaacca gaccactca gagcacctt ccctcaaag cctgagtgg cagcgagac tgacatttc cacctcaaa tgaaaacct ctcgaacga agaaaagta cgtgatacg tcacaccgc ccgccagga gccccaagg ccaggagaa ccagcaagc ctcaaatcg cagctccct gcgagacag cctcccctc gaaccgcag ctcgctgga cgtctcctc ctcctcgta caacaccct catcatcca cagcgcggg ggacgagcc ctggacagg acgcaactc cggtgtgag ggaatcggg cgacgccta ccaccccca ccaaccctc gaatctgta cgaccccaa actctcgcc caatcagaa gcgaagctc cgcctcgcc ctcgccctc cgtccggac gagctttga cggaaacac tcgaatcta cgacaccgg ggtccagga aatcaccga aatccccga tgactatct cagccaatc ctcagtcct caaacactt ggccaaaga agtcaaggt cccgtcccc caaatccga cttttcctt cttcgataa cccgccccc tgagtaccc aaagtgggc cgataagtc gccgttaca ggcggctgt gcatcgaaa taacgtcaa taacgagaa attgacact ctcgaagtc gcaacccga cctgtccaa ggtgggcgt gcccaaagg ggcggccga cttcgatgg gttcaggcg gggcgtgtc cgcgccgag accgcccac caaaggcag ggaggaggc ggagacagg gggcgggga gttgtggcc ctcagccga aatggtcga aatactcat caaggagaa cagtgcctt gaaaatcga attggagaa ttgctacaa aagagttgc aaagacaca aaagcttga acaagaaat tctaaaagt acacaaggc acaggaaga gttagttca gtcgtgcga gcgcaaaga acggctcga acgagccgc tagggggcg tttgcaaaa tgacaatcg ccggttgca agagttgaa tcgggccct gcgtgagca agttgattt gttatcaac tcaaatgtt gtcaaggtc accggcgcc cgattcagg ctcacccga ttgtttgcg gagagagtt gagcaagag ggaggtgtt gatcgctca gttgattca gcagaataa ggaacatgt tgctgcgaa agaacgcca agaaatcga gttggccgc gcaaagagt tacacttca ggaacaacg cacccatat cgatattct agacaccgc acttaccaa cgctcaagc gaatgtcgt ccgtttgga ggaagaatg ccgcaaaaa gcaagtgta cgtggagcg cgtggtgca gctacagag ggcgctgtc gtcgctcca gcttgcgag cgaccgtcg cgagcaaac cgagcgcaa attgcgttt gcagctcga gcgcgagct ccagcgcac caaactggc gcaaggcga gggcggtaa aagtggctg ggattcccc gaatggttc gggtaacga atcaccgtc cgaattaaa acgacgcct gcgcgaaaa agaggagaa aatcatgca attggaggg cgattgcac caagtggga gcagaggta tttggagga gagtgcgtt gagacaggc ggccatcga cgcggcaag cattcccaa ggatgcgaa aattgccgc cctggagaa gaccagtca agagacgga gaaaatcat cgccgaggc gagaaacga gaaaattag acatatgga cgaagtcca tgccgccca aaagaaagt tgccgattt ggagtcgcg tgttaaaga tctcgaatc gaagttggc tgagcgcga cgctatgat taaagtatt gcagaagca tacgtacga taaggacgt atcgagtat gctggggcg gtcccccca ccatacgcc ccaccccag tctgggcgg ggccgacat tgatcacgt gttgggaac ggcggtgtc gagggagga gttagtcag tagcgtcct aacgagttc taccggttt tggatcggg gaattccta caccgggag tgattcgtc ctaccatat gccttctta taccaaata tgaaaataa aagttttga ttcgacgaa acaaagttt ggataatca gctaaaaga aatcgatag tcaactcga ttcgcaact gttgagcaa gcggggtct ttgctgttt tccaggatt cactaatcc agtcactgc gcagagaaa aggaaaaat acccaagcc actattggc gggtgtgac cggcggcgg cgctggggg cggagtcgc gatgtacga gcccggcgc gatgctcct gttggagaa gcagggccg cgcctccca gcagcaacg catgcagga cactgccga cgcctcgcc gcgctgccc cgcccccag ctcgctgcc gcgtccccc gcgcccctc ccaccgcag cggcctgcc gcgccgcct cggcgacta caataggct agccgaggg cgagactcc gccccggaa gaagtccga cggcgccga ttcgctcag ttccaacgc cagcagccg gagggcgtc gccggcgcg ctcgctgat cccgccccg gaagctggg cgaatacgg gaggctgtc agagagtgg cggctcgag cgcctccct ccgcaaacc gaccaatca gagatcgag gagtggcgg cgagagaga ctccgcctc cacgctcag cgataactc ggccagttc cttgccaac gccctccaa agtgaggct cttcggggg cggggcgat gagaagagg cggctcttt ggggcggga cagcaaaag ctccaccga ttcgacaac gagcaagta tcgtataca gttttaggt tagaagcgg cttccgcta tcatgtgcc tgatttaaa tattttgga ctttttgtc cgaattgta gctagttag gttgcctcg atgttattt tgtctttac cgggtttct tttgttttt acgcttgtt attattgta ttgtagaac agtctatag tcagtcttt acttactaa ctagtctta ttaaaccaa atagatcaa agctaggga ccaaatttt ttgttccag tggcagatt gttgtttct ttattattt tctttttta tgttaggta aagacaaat aaggccata tcctacaaa gtgccttca gtagataaa caaggtctc tcgagaaag tgtactagt ggtttaatt gaaattttc tgggcagta tttttcaat aaatttcat attatcagt tgaaatttt taaagtgca ttgattatt tggcataaa tcaaatgtt ccgtccgtt tagtaaaat acaggctgt tccgtctaa accgcgcat tactgaaaa tgttaatag tgattgtgc aggagctgc gggattatg gttgtatac aacttccaa tacttctaa aatagggac accctgtat aatattccg gaaaattcg tattgggcc actctatgg attcgaatc tcctattta cactttttt atacaaagt tttcttgtt tctagtaat acagtctag tctgtttat acaaaaaat gtattcgaa atgtacaga caataaatc agttttacc aac
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