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Gene information
General information
TC011304 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 19273100..19275714
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011304
    iBeetle screen iB_05009

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011304 RA
      MIKFPLGDA DSKEKFFLS NSFGAVDGA ELIPIHCGG 
      RLITPTVSV NELGKQLLK AAAEGNVEE TRTLLNKGA 
      LFTADWLGT SPLHLAARN NHLEVVEIL LKAGISRDA 
      RTKVDKTSL HMAAAEGHV SIVETLLEY GSDPDCRDL 
      LGMTPLHWA AQNGHLDVV KALLQHYAQ TNVVNKFDL 
      TPAGVAAQI DRHDIVALI NRQSEEVEE PTNAAQHLT 
      LELVENDEG ALDGAHSPI PLETILMED NESSIDPEK 
      LHLNKNPDE QNHDFSESI KLLQEHGIT MLPNDDSNI 
      LTTVMETGH SVVLTDAGK QVLNAIKES EQKANAINK 
      KIITVTPQE FLSMASNCK NKSKNIIKQ INGRLVPKN 
      VKRIVMKKN KLIPVSTVN NMYKISGPN GVTDMETVM 
      TQLIEARKT IEEYKIKLL KKEQEAERY KQQLKLLMD 
      DS
    CDS sequence
    • >TC011304 RA
      atgataaaa ttcccgctc ggagacgcc gattcgaag 
      gagaaattt ttcctctcg aacagcttc ggggctgtg 
      gatggggcc gaattaatc ccaatacac tgtgggggc 
      aggttgatc acaccaacg gtttcagtc aacgaattg 
      ggaaaacag ctactgaaa gcggcagct gaaggcaac 
      gtcgaggag acaaggacg ctgctgaac aagggggcc 
      cttttcacg gccgattgg ttggggacc agtccgctg 
      catctggcg gcgcgtaac aaccacttg gaagtggtt 
      gaaatccta ctgaaggcg gggatttcg cgcgatgct 
      cgcactaaa gtcgacaag acttcgctg catatggcg 
      gctgctgag ggtcacgtg agtattgtt gagacgctt 
      cttgagtat ggctcggat cccgactgt cgcgatttg 
      ttagggatg acgccgttg cattgggcg gcccaaaac 
      ggacacttg gacgtggtc aaggccctc ttgcaacat 
      tatgcgcaa acgaacgtt gtcaataag ttcgatttg 
      acgccggca ggtgtcgct gcgcagata gaccggcat 
      gatatagtg gcgttaatt aatcgacaa agtgaggaa 
      gtggaagag cctacgaat gcagcccag catctgacc 
      ttggagttg gtggaaaat gatgagggg gctttggat 
      ggggcacat tcgccgatt ccgttagaa actatttta 
      atggaagac aatgaaagt tcaatagac cccgaaaag 
      ctacatttg aataaaaac ccagatgag caaaaccac 
      gacttctcc gagtcgatt aaattattg caagagcac 
      ggaattacg atgctccct aatgacgac agcaacatt 
      ttaacgacg gttatggag actggacat tcggttgtg 
      ttaacggat gccggaaaa caggtttta aacgcaatc 
      aaagagtca gaacagaag gcgaatgcc ataaataaa 
      aagatcata accgtcaca ccacaagaa tttttgtca 
      atggcgtca aattgtaag aacaagtcc aaaaacata 
      attaaacaa atcaacgga cggctcgtt ccaaagaat 
      gtgaaacgg attgtcatg aagaagaat aaactgatc 
      ccagtctcg actgtaaat aatatgtat aaaatctcg 
      ggtcctaac ggcgtcacg gacatggag acagtgatg 
      acgcagttg atcgaggcg cgaaaaacg atagaagaa 
      tacaaaata aaactgctg aaaaaagag caagaagcc 
      gagcggtac aaacagcaa ttaaaattg ttaatggat 
      gatagctag
    mRNA sequence
    • >TC011304 RA
      cggatggga gtaaccaaa aagtaagaa cgccatgac 
      gtttggtat ttacagtgt tggcataat ttaggctca 
      aattcgcga tcttggcat taaaataaa cccctaatc 
      tctaatcta tcatgataa aattcccgc tcggagacg 
      ccgattcga aggagaaat ttttcctct cgaacagct 
      tcggggctg tggatgggg ccgaattaa tcccaatac 
      actgtgggg gcaggttga tcacaccaa cggtttcag 
      tcaacgaat tgggaaaac agctactga aagcggcag 
      ctgaaggca acgtcgagg agacaagga cgctgctga 
      acaaggggg cccttttca cggccgatt ggttgggga 
      ccagtccgc tgcatctgg cggcgcgta acaaccact 
      tggaagtgg ttgaaatcc tactgaagg cggggattt 
      cgcgcgatg ctcgcacta aagtcgaca agacttcgc 
      tgcatatgg cggctgctg agggtcacg tgagtattg 
      ttgagacgc ttcttgagt atggctcgg atcccgact 
      gtcgcgatt tgttaggga tgacgccgt tgcattggg 
      cggcccaaa acggacact tggacgtgg tcaaggccc 
      tcttgcaac attatgcgc aaacgaacg ttgtcaata 
      agttcgatt tgacgccgg caggtgtcg ctgcgcaga 
      tagaccggc atgatatag tggcgttaa ttaatcgac 
      aaagtgagg aagtggaag agcctacga atgcagccc 
      agcatctga ccttggagt tggtggaaa atgatgagg 
      gggctttgg atggggcac attcgccga ttccgttag 
      aaactattt taatggaag acaatgaaa gttcaatag 
      accccgaaa agctacatt tgaataaaa acccagatg 
      agcaaaacc acgacttct ccgagtcga ttaaattat 
      tgcaagagc acggaatta cgatgctcc ctaatgacg 
      acagcaaca ttttaacga cggttatgg agactggac 
      attcggttg tgttaacgg atgccggaa aacaggttt 
      taaacgcaa tcaaagagt cagaacaga aggcgaatg 
      ccataaata aaaagatca taaccgtca caccacaag 
      aatttttgt caatggcgt caaattgta agaacaagt 
      ccaaaaaca taattaaac aaatcaacg gacggctcg 
      ttccaaaga atgtgaaac ggattgtca tgaagaaga 
      ataaactga tcccagtct cgactgtaa ataatatgt 
      ataaaatct cgggtccta acggcgtca cggacatgg 
      agacagtga tgacgcagt tgatcgagg cgcgaaaaa 
      cgatagaag aatacaaaa taaaactgc tgaaaaaag 
      agcaagaag ccgagcggt acaaacagc aattaaaat 
      tgttaatgg atgatagct agtatcgct gatttgcca 
      tatttttaa ttgaatggg ttataaata gcgcaattt 
      gtccggatt ttaccggaa atgtgctgt tggtggctc 
      gttatctga agcgtaatt gaacttaat tactatttt 
      tgtacatta atattcgag tcttcttgt attttgcga 
      gtgataact tgtgtaatt tatttgttt ttaattttt 
      tgtaatgtt gttgaacta atgttgata aaaaagaac 
      gaaatgaaa gactt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY