Status
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Gene information
General information
TC031361
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011297,TC011298
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 19462919..19504292
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031361
    iBeetle screen iB_05008

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: 3 - Number of animals showing the phenotype: 4
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 4 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Pupal morphology
    wings slightly size shortened - Number of animals showing the phenotype: 9

    oblique dorsal thoracic muscles fragmented - Number of animals showing the phenotype: 7

    Comment: the outer oblique muscles is fragmented, the median one is ok
    Adult morphology
    elytra not closed - Number of animals showing the phenotype: 2


    elytra surface irregular - Number of animals showing the phenotype: 2


    elytra slightly size shortened - Number of animals showing the phenotype: 6


    Fertility
    egg production: number no/almost no eggs -
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031361 RA
      MTTPAKLYG KDLSEYDEI DVDELLNKL SAEEINILA 
      KEVDPDDTF LPPSQRCSY DCEKEATGP LNRKQLIEH 
      INKQAIETP DKPELKPYV PGTVRGKKW IPPPPPVKE 
      QEADEQIAV DLGDEYEQA LSLATQEEI IDLAAILGF 
      HSMMNQDQY HASLLNKGQ PVGLGWDGI TKASKQKIY 
      PMDPPNMTD PDETIKKVQ DDDPKFIDL NWNNIKNIS 
      DEKFDSLFE ALENNTHLE TMSLANVGL TDRGAEKLA 
      QALEKNNTV RVVNVETNL ISPSGIVRL IKSLLKTKA 
      VEEFRASNQ RSQVLGNKI EMEITQLIE KNPTLLRLG 
      LHLEYNDAR HRIASHLQR NIDTKRRLS RLGATSS
    • >TC031361 RB
      MAETVEETY ETEVRRKVI RTSLKIEET SSTTKTTTM 
      TTPAKLYGK DLSEYDEID VDELLNKLS AEEINILAK 
      EVDPDDTFL PPSQRCSYD CEKEATGPL NRKQLIEHI 
      NKQAIETPD KPELKPYVP GTVRGKKWI PPPPPVKEQ 
      EADEQIAVD LGDEYEQAL SLATQEEII DLAAILGFH 
      SMMNQDQYH ASLLNKGQP VGLGWDGIT KASKQKIYP 
      MDPPNMTDP DETIKKVQD DDPKFIDLN WNNIKNISD 
      EKFDSLFEA LENNTHLET MSLANVGLT DRGAEKLAQ 
      ALEKNNTVR VVNVETNLI SPSGIVRLI KSLLKTKAV 
      EEFRASNQR SQVLGNKIE MEITQLIEK NPTLLRLGL 
      HLEYNDARH RIASHLQRN IDTKRRLSR LGATSS
    CDS sequence
    • >TC031361 RA
      atgacaacg ccagccaaa ctctatggc aaagatctc 
      agtgaatac gacgaaata gatgtagac gaacttttg 
      aataagtta tcagctgaa gagattaac attctagcc 
      aaagaagta gaccctgat gacacattt ttgccaccc 
      tcacagagg tgtagttat gactgtgag aaggaagca 
      acggggccc ctaaaccgc aaacaactc atcgaacac 
      ataaataaa caagcaatt gagactccc gacaagccc 
      gaactcaaa ccatatgta cctggaaca gtccgcggc 
      aaaaaatgg ataccgcca ccgccccct gtcaaggaa 
      caagaagct gacgagcaa atagctgta gatttaggc 
      gacgagtac gagcaagct ttatcttta gccacacag 
      gaggaaatt attgatttg gcggctatt ttgggtttc 
      cattcgatg atgaatcag gaccagtat cacgcatca 
      cttctcaat aagggtcag ccggtcggg ttgggctgg 
      gacggcatc acgaaagcc tcaaaacag aaaatatac 
      cccatggac ccgcccaat atgacggat ccagacgaa 
      acaataaaa aaagttcag gatgatgac cccaaattc 
      atcgacctc aattggaac aatatcaag aatatatca 
      gacgagaag tttgacagt ttgtttgaa gcgttggag 
      aataatacg catttggag acgatgtcg ttagcgaac 
      gtcggattg actgatcga ggggccgag aagttggcg 
      caagcttta gaaaaaaat aatactgtg agggtcgtt 
      aatgtagaa actaatctg atttcacct agtgggatc 
      gtgcggctg attaagtcg ctgttgaag acgaaggcg 
      gtggaggag tttagagcg tcgaatcag aggtcgcag 
      gtgttgggt aacaagata gagatggag atcacgcag 
      ttaatagaa aagaacccg acgttatta aggttgggc 
      ttgcattta gaatacaac gatgcccgt catcgtatc 
      gcctcacat ttacaacgt aatattgac acaaaacgc 
      cgcctgtct cggctgggg gcgaccagc tcatag
    • >TC031361 RB
      atggccgag acggtcgag gaaacctac gagacggaa 
      gtccggcga aaagtcatc cggacaagc ctcaaaatc 
      gaagaaacg tcgtcgacg acgaaaaca acaacaatg 
      acaacgcca gccaaactc tatggcaaa gatctcagt 
      gaatacgac gaaatagat gtagacgaa cttttgaat 
      aagttatca gctgaagag attaacatt ctagccaaa 
      gaagtagac cctgatgac acatttttg ccaccctca 
      cagaggtgt agttatgac tgtgagaag gaagcaacg 
      gggccccta aaccgcaaa caactcatc gaacacata 
      aataaacaa gcaattgag actcccgac aagcccgaa 
      ctcaaacca tatgtacct ggaacagtc cgcggcaaa 
      aaatggata ccgccaccg ccccctgtc aaggaacaa 
      gaagctgac gagcaaata gctgtagat ttaggcgac 
      gagtacgag caagcttta tctttagcc acacaggag 
      gaaattatt gatttggcg gctattttg ggtttccat 
      tcgatgatg aatcaggac cagtatcac gcatcactt 
      ctcaataag ggtcagccg gtcgggttg ggctgggac 
      ggcatcacg aaagcctca aaacagaaa atatacccc 
      atggacccg cccaatatg acggatcca gacgaaaca 
      ataaaaaaa gttcaggat gatgacccc aaattcatc 
      gacctcaat tggaacaat atcaagaat atatcagac 
      gagaagttt gacagtttg tttgaagcg ttggagaat 
      aatacgcat ttggagacg atgtcgtta gcgaacgtc 
      ggattgact gatcgaggg gccgagaag ttggcgcaa 
      gctttagaa aaaaataat actgtgagg gtcgttaat 
      gtagaaact aatctgatt tcacctagt gggatcgtg 
      cggctgatt aagtcgctg ttgaagacg aaggcggtg 
      gaggagttt agagcgtcg aatcagagg tcgcaggtg 
      ttgggtaac aagatagag atggagatc acgcagtta 
      atagaaaag aacccgacg ttattaagg ttgggcttg 
      catttagaa tacaacgat gcccgtcat cgtatcgcc 
      tcacattta caacgtaat attgacaca aaacgccgc 
      ctgtctcgg ctgggggcg accagctca tag
    mRNA sequence
    • >TC031361 RA
      cgattaatc agacaatca ataaaataa aaaattcgg 
      gtcacaacg agtgttggg tgtgtaaaa ccatcaatt 
      tcctttata tttcatcag acgtcgtcg acgacgaaa 
      acaacaaca atgacaacg ccagccaaa ctctatggc 
      aaagatctc agtgaatac gacgaaata gatgtagac 
      gaacttttg aataagtta tcagctgaa gagattaac 
      attctagcc aaagaagta gaccctgat gacacattt 
      ttgccaccc tcacagagg tgtagttat gactgtgag 
      aaggaagca acggggccc ctaaaccgc aaacaactc 
      atcgaacac ataaataaa caagcaatt gagactccc 
      gacaagccc gaactcaaa ccatatgta cctggaaca 
      gtccgcggc aaaaaatgg ataccgcca ccgccccct 
      gtcaaggaa caagaagct gacgagcaa atagctgta 
      gatttaggc gacgagtac gagcaagct ttatcttta 
      gccacacag gaggaaatt attgatttg gcggctatt 
      ttgggtttc cattcgatg atgaatcag gaccagtat 
      cacgcatca cttctcaat aagggtcag ccggtcggg 
      ttgggctgg gacggcatc acgaaagcc tcaaaacag 
      aaaatatac cccatggac ccgcccaat atgacggat 
      ccagacgaa acaataaaa aaagttcag gatgatgac 
      cccaaattc atcgacctc aattggaac aatatcaag 
      aatatatca gacgagaag tttgacagt ttgtttgaa 
      gcgttggag aataatacg catttggag acgatgtcg 
      ttagcgaac gtcggattg actgatcga ggggccgag 
      aagttggcg caagcttta gaaaaaaat aatactgtg 
      agggtcgtt aatgtagaa actaatctg atttcacct 
      agtgggatc gtgcggctg attaagtcg ctgttgaag 
      acgaaggcg gtggaggag tttagagcg tcgaatcag 
      aggtcgcag gtgttgggt aacaagata gagatggag 
      atcacgcag ttaatagaa aagaacccg acgttatta 
      aggttgggc ttgcattta gaatacaac gatgcccgt 
      catcgtatc gcctcacat ttacaacgt aatattgac 
      acaaaacgc cgcctgtct cggctgggg gcgaccagc 
      tcatagtgt gctgtggtc gcgattacg tacgtacct 
      actgttttt aataactac aacctgccc aataaaatt 
      gtttttgga ct
    • >TC031361 RB
      tccgttcag tcaagaaaa ttaatttta gcctgacgc 
      ttcagtctt gcacgcgtt tccacggcg aatagttcg 
      agtgagcaa agtgccgaa ttttcccct tgtaaactg 
      tgacaacct ccccagcga tggccgaga cggtcgagg 
      aaacctacg agacggaag tccggcgaa aagtcatcc 
      ggacaagcc tcaaaatcg aagaaacgt cgtcgacga 
      cgaaaacaa caacaatga caacgccag ccaaactct 
      atggcaaag atctcagtg aatacgacg aaatagatg 
      tagacgaac ttttgaata agttatcag ctgaagaga 
      ttaacattc tagccaaag aagtagacc ctgatgaca 
      catttttgc caccctcac agaggtgta gttatgact 
      gtgagaagg aagcaacgg ggcccctaa accgcaaac 
      aactcatcg aacacataa ataaacaag caattgaga 
      ctcccgaca agcccgaac tcaaaccat atgtacctg 
      gaacagtcc gcggcaaaa aatggatac cgccaccgc 
      cccctgtca aggaacaag aagctgacg agcaaatag 
      ctgtagatt taggcgacg agtacgagc aagctttat 
      ctttagcca cacaggagg aaattattg atttggcgg 
      ctattttgg gtttccatt cgatgatga atcaggacc 
      agtatcacg catcacttc tcaataagg gtcagccgg 
      tcgggttgg gctgggacg gcatcacga aagcctcaa 
      aacagaaaa tatacccca tggacccgc ccaatatga 
      cggatccag acgaaacaa taaaaaaag ttcaggatg 
      atgacccca aattcatcg acctcaatt ggaacaata 
      tcaagaata tatcagacg agaagtttg acagtttgt 
      ttgaagcgt tggagaata atacgcatt tggagacga 
      tgtcgttag cgaacgtcg gattgactg atcgagggg 
      ccgagaagt tggcgcaag ctttagaaa aaaataata 
      ctgtgaggg tcgttaatg tagaaacta atctgattt 
      cacctagtg ggatcgtgc ggctgatta agtcgctgt 
      tgaagacga aggcggtgg aggagttta gagcgtcga 
      atcagaggt cgcaggtgt tgggtaaca agatagaga 
      tggagatca cgcagttaa tagaaaaga acccgacgt 
      tattaaggt tgggcttgc atttagaat acaacgatg 
      cccgtcatc gtatcgcct cacatttac aacgtaata 
      ttgacacaa aacgccgcc tgtctcggc tgggggcga 
      ccagctcat agtgtgctg tggtcgcga ttacgtacg 
      tacctactg tttttaata actacaacc tgcccaata 
      aaattgttt ttggact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY