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Gene information
General information
TC031351
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011272
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 1804351..1814329
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC031351
      iBeetle screen iB_05007

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection


      eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 2 (16 dpi)
      Days post injection



      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (16 dpi)
      Days post injection



      Adult morphology
      elytra surface irregular - Number of animals showing the phenotype: 3


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC031351 RA
        MLKQGIIRP SDSPWSSPI WIVPKKLDA SGQRKWRIV 
        IDYRKLNEQ TVEDRYPLP NINDILDKL GRSQYFSTI 
        DLASGFHQI EVDPNDVQK TAFTVENGH YEFVRMPFG 
        LKNAPSTFQ RVMDNVLRG ILNRLQTLE FVPEINQYL 
        GIFPVSVPI KSSCSNVLA QHQIQGVFL FENQLNCQK 
        YVNDQLLIF NDTTERKPI ILEDFRLPV RNNVKIPKL 
        TLRTLQVTS LTNNLPRLQ MYPYEDSKN IWHLTGTAI 
        LYFVFLALA IWFMMQKIA FRRRNQTEE APEPVELPR 
        DAKF
      CDS sequence
      • >TC031351 RA
        atgctaaaa caaggcata atccgacct tctgattcg 
        ccatggtcc tctccaatt tggatagta cccaaaaaa 
        ttagacgct tccgggcaa cgcaaatgg agaatagtc 
        atcgactat cgaaaactt aacgaacaa acagtcgaa 
        gatcgttac cccttacca aatatcaat gacatactc 
        gataagctg ggtcgtagt caatacttt agcacaatc 
        gatcttgct tccggtttc caccaaata gaagtagac 
        ccaaacgat gtccaaaaa actgcattt acggtagaa 
        aatgggcat tacgaattc gtcagaatg cccttcggc 
        ttgaaaaac gccccatca acattccaa cgtgtgatg 
        gacaatgta ttacgcggt atcctcaac aggctccaa 
        actctcgaa ttcgttccc gaaattaat cagtatcta 
        ggcattttt ccggtatca gttcccatc aaaagttct 
        tgctccaat gttttggca caacaccaa atccaaggt 
        gtcttcctc ttcgaaaat caactaaac tgccaaaaa 
        tacgtcaat gatcaactt ctgattttt aatgataca 
        accgaaaga aaacccata atcctagaa gacttcaga 
        cttcctgta agaaataat gtgaagatt cccaagcta 
        accttgagg acccttcaa gttacaagt ttaactaac 
        aacctgccc cgacttcag atgtacccc tacgaagat 
        agcaaaaat atttggcac ctaaccgga actgctata 
        ttgtatttt gtatttcta gccttagcc atctggttt 
        atgatgcag aagatagca tttcggcga agaaaccaa 
        acggaagaa gcccctgag ccagtagaa ctaccccgc 
        gatgccaaa ttttag
      mRNA sequence
      • >TC031351 RA
        tttgattac agacgtaga cgattttca gattaccca 
        aataccatt cgaaagtgt ctgtggccc ggacacgat 
        gaaactttg gcttaacaa taacgaata agtaacacg 
        tttctgata ttactctta tacccaatt ttgtccttt 
        tcccgaata ttgtcattt caccattac ttatgaatt 
        tataaactg agtttcttg tttatgtgt aacttccaa 
        cttgtttat gaataactt acgaattat gaactgatt 
        taataaatt atgaatttt ccaatgtat ttatgagtg 
        catgattat gatacattt atgtgaaaa aaaagagag 
        atacagatt atgaattat gatatcctc agtacttat 
        ttgacgttt tgttcatag ggtttgttg gacagaaat 
        attattatg tatgtacac tgtatgtta ggaaactag 
        caatttagg ttcttttgt gttgaacaa atgatttgc 
        aatgtttta gttctttag tattctgtt ttacatgtc 
        ttatttttg tgatcgcgg acgatcacc agtcaggat 
        ggccgagtt gtaggattt gtatctccc gccactggc 
        tggggtcct ccgcgacta gaaaaagca aaactttac 
        tttgctaaa ttaataaaa aatgacccg tttattatt 
        tctacagcg cacggctgt acaaaagaa caatttagc 
        gtttccata ccctgtccc aaaattttt aaaagtaaa 
        actaaaatc gttaagttc catatattc aaatttcac 
        aaatatttc gattgttta cttggcatt gataattta 
        aaacaactc caagcaaac attgacctt aacttgggc 
        aaactcttt cttcccaat acccaaatc gatataaac 
        ttaaaaaaa ttatgtgat tacaataat catatcgaa 
        gtcctgcca aattccgaa caagtaata aaaattaag 
        attgcaaac gttaaaaat ggaactgcc cttttacca 
        aaacataca ttttcagac ctagaaatt cacgaaagt 
        ctcattaaa gtcgaaaat aatttcgct ttaacgtcg 
        ttaataaac cctactaac aaacataca aaagtcgac 
        atctctcat ccatttgaa gtgattcca caggaccaa 
        tttgatata ccaaattta aacaacctt gaacctcaa 
        gcacaaaat tttgatttt tccaaagta agattagac 
        cactgtaac aaagaagag cgtcaaaat ttagtaaag 
        ttgctgaaa tcttattca gacattttt tattttgat 
        tctgacaac ctaacattt acaaatgca gtgaaacac 
        aaaattaat ttgaaggac gagactccc atacacact 
        aaaacatat cgttacccc cacattcac aaaacggag 
        gtacaaaat caaatatcc aaaatgcta aaacaaggc 
        ataatccga ccttctgat tcgccatgg tcctctcca 
        atttggata gtacccaaa aaattagac gcttccggg 
        caacgcaaa tggagaata gtcatcgac tatcgaaaa 
        cttaacgaa caaacagtc gaagatcgt tacccctta 
        ccaaatatc aatgacata ctcgataag ctgggtcgt 
        agtcaatac tttagcaca atcgatctt gcttccggt 
        ttccaccaa atagaagta gacccaaac gatgtccaa 
        aaaactgca tttacggta gaaaatggg cattacgaa 
        ttcgtcaga atgcccttc ggcttgaaa aacgcccca 
        tcaacattc caacgtgtg atggacaat gtattacgc 
        ggtatcctc aacaggctc caaactctc gaattcgtt 
        cccgaaatt aatcagtat ctaggcatt tttccggta 
        tcagttccc atcaaaagt tcttgctcc aatgttttg 
        gcacaacac caaatccaa ggtgtcttc ctcttcgaa 
        aatcaacta aactgccaa aaatacgtc aatgatcaa 
        cttctgatt tttaatgat acaaccgaa agaaaaccc 
        ataatccta gaagacttc agacttcct gtaagaaat 
        aatgtgaag attcccaag ctaaccttg aggaccctt 
        caagttaca agtttaact aacaacctg ccccgactt 
        cagatgtac ccctacgaa gatagcaaa aatatttgg 
        cacctaacc ggaactgct atattgtat tttgtattt 
        ctagcctta gccatctgg tttatgatg cagaagata 
        gcatttcgg cgaagaaac caaacggaa gaagcccct 
        gagccagta gaactaccc cgcgatgcc aaattttag 
        ttcctctcc ggagtttcg ggctaagag gggaggaat 
        tatatgtgc aaaatatcc ccttagtgc ttaatgctt 
        agtaaacat tttttatat tataaatat atgctaaat 
        tgtaga
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY