General information | |||
TC031351
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007425.3: 1804351..1814329 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC031351 |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
Gene | {{ goGene }} |
|
---|---|---|
GO ID | * {{ blurIdResult }} |
|
Evidence | {{ blurEvidenceResult }} |
|
Reference | * {{ blurReferenceResult }} |
|
Quotation | ||
Name | ||
Publish name | ||
{{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_05007 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 2 (16 dpi)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (16 dpi)
- >TC031351 RA
MLKQGIIRP SDSPWSSPI WIVPKKLDA SGQRKWRIV IDYRKLNEQ TVEDRYPLP NINDILDKL GRSQYFSTI DLASGFHQI EVDPNDVQK TAFTVENGH YEFVRMPFG LKNAPSTFQ RVMDNVLRG ILNRLQTLE FVPEINQYL GIFPVSVPI KSSCSNVLA QHQIQGVFL FENQLNCQK YVNDQLLIF NDTTERKPI ILEDFRLPV RNNVKIPKL TLRTLQVTS LTNNLPRLQ MYPYEDSKN IWHLTGTAI LYFVFLALA IWFMMQKIA FRRRNQTEE APEPVELPR DAKF
- >TC031351 RA
atgctaaaa caaggcata atccgacct tctgattcg ccatggtcc tctccaatt tggatagta cccaaaaaa ttagacgct tccgggcaa cgcaaatgg agaatagtc atcgactat cgaaaactt aacgaacaa acagtcgaa gatcgttac cccttacca aatatcaat gacatactc gataagctg ggtcgtagt caatacttt agcacaatc gatcttgct tccggtttc caccaaata gaagtagac ccaaacgat gtccaaaaa actgcattt acggtagaa aatgggcat tacgaattc gtcagaatg cccttcggc ttgaaaaac gccccatca acattccaa cgtgtgatg gacaatgta ttacgcggt atcctcaac aggctccaa actctcgaa ttcgttccc gaaattaat cagtatcta ggcattttt ccggtatca gttcccatc aaaagttct tgctccaat gttttggca caacaccaa atccaaggt gtcttcctc ttcgaaaat caactaaac tgccaaaaa tacgtcaat gatcaactt ctgattttt aatgataca accgaaaga aaacccata atcctagaa gacttcaga cttcctgta agaaataat gtgaagatt cccaagcta accttgagg acccttcaa gttacaagt ttaactaac aacctgccc cgacttcag atgtacccc tacgaagat agcaaaaat atttggcac ctaaccgga actgctata ttgtatttt gtatttcta gccttagcc atctggttt atgatgcag aagatagca tttcggcga agaaaccaa acggaagaa gcccctgag ccagtagaa ctaccccgc gatgccaaa ttttag
- >TC031351 RA
tttgattac agacgtaga cgattttca gattaccca aataccatt cgaaagtgt ctgtggccc ggacacgat gaaactttg gcttaacaa taacgaata agtaacacg tttctgata ttactctta tacccaatt ttgtccttt tcccgaata ttgtcattt caccattac ttatgaatt tataaactg agtttcttg tttatgtgt aacttccaa cttgtttat gaataactt acgaattat gaactgatt taataaatt atgaatttt ccaatgtat ttatgagtg catgattat gatacattt atgtgaaaa aaaagagag atacagatt atgaattat gatatcctc agtacttat ttgacgttt tgttcatag ggtttgttg gacagaaat attattatg tatgtacac tgtatgtta ggaaactag caatttagg ttcttttgt gttgaacaa atgatttgc aatgtttta gttctttag tattctgtt ttacatgtc ttatttttg tgatcgcgg acgatcacc agtcaggat ggccgagtt gtaggattt gtatctccc gccactggc tggggtcct ccgcgacta gaaaaagca aaactttac tttgctaaa ttaataaaa aatgacccg tttattatt tctacagcg cacggctgt acaaaagaa caatttagc gtttccata ccctgtccc aaaattttt aaaagtaaa actaaaatc gttaagttc catatattc aaatttcac aaatatttc gattgttta cttggcatt gataattta aaacaactc caagcaaac attgacctt aacttgggc aaactcttt cttcccaat acccaaatc gatataaac ttaaaaaaa ttatgtgat tacaataat catatcgaa gtcctgcca aattccgaa caagtaata aaaattaag attgcaaac gttaaaaat ggaactgcc cttttacca aaacataca ttttcagac ctagaaatt cacgaaagt ctcattaaa gtcgaaaat aatttcgct ttaacgtcg ttaataaac cctactaac aaacataca aaagtcgac atctctcat ccatttgaa gtgattcca caggaccaa tttgatata ccaaattta aacaacctt gaacctcaa gcacaaaat tttgatttt tccaaagta agattagac cactgtaac aaagaagag cgtcaaaat ttagtaaag ttgctgaaa tcttattca gacattttt tattttgat tctgacaac ctaacattt acaaatgca gtgaaacac aaaattaat ttgaaggac gagactccc atacacact aaaacatat cgttacccc cacattcac aaaacggag gtacaaaat caaatatcc aaaatgcta aaacaaggc ataatccga ccttctgat tcgccatgg tcctctcca atttggata gtacccaaa aaattagac gcttccggg caacgcaaa tggagaata gtcatcgac tatcgaaaa cttaacgaa caaacagtc gaagatcgt tacccctta ccaaatatc aatgacata ctcgataag ctgggtcgt agtcaatac tttagcaca atcgatctt gcttccggt ttccaccaa atagaagta gacccaaac gatgtccaa aaaactgca tttacggta gaaaatggg cattacgaa ttcgtcaga atgcccttc ggcttgaaa aacgcccca tcaacattc caacgtgtg atggacaat gtattacgc ggtatcctc aacaggctc caaactctc gaattcgtt cccgaaatt aatcagtat ctaggcatt tttccggta tcagttccc atcaaaagt tcttgctcc aatgttttg gcacaacac caaatccaa ggtgtcttc ctcttcgaa aatcaacta aactgccaa aaatacgtc aatgatcaa cttctgatt tttaatgat acaaccgaa agaaaaccc ataatccta gaagacttc agacttcct gtaagaaat aatgtgaag attcccaag ctaaccttg aggaccctt caagttaca agtttaact aacaacctg ccccgactt cagatgtac ccctacgaa gatagcaaa aatatttgg cacctaacc ggaactgct atattgtat tttgtattt ctagcctta gccatctgg tttatgatg cagaagata gcatttcgg cgaagaaac caaacggaa gaagcccct gagccagta gaactaccc cgcgatgcc aaattttag ttcctctcc ggagtttcg ggctaagag gggaggaat tatatgtgc aaaatatcc ccttagtgc ttaatgctt agtaaacat tttttatat tataaatat atgctaaat tgtaga
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
