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Gene information
General information
TC011263 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 1280906..1296280
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011263
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05006

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011263 RA
      MADPLSLLR QYNVNKKEI IEREGQIIF GEFSWPKNV 
      KTNYLMWGS GKDGTPKEY YTLECLLFI LKNVSLTHP 
      VYVRQAAAE NIPVVRRPD RKELLAYLN GETASCPAI 
      DKSAPLEIP TQVKRSADY EGPESIAKK PRFEDLHVQ 
      KVREKLAAR LDAPKEASV TVDNIKSLS EAMSVEKIA 
      AIKAKRLAK KRTTIKGND DIGQEYDIR AILDLDVDL 
      TKDIISRER QWRTRTTIL QSNGKTFAK NILAMLHSI 
      KAREEGRQR PPQQAPIVT PRQTPVRPT TQPAVYNRY 
      DQERFNRQK EETEGFKID TMGTYHGMT LKSVTEGPV 
      KARPQPIQP QTPLPVSAA RPPSGGLTK RVSRTPIII 
      IPAGTNSLI SMYNAKEIL QDLKFVSVE QKKNEGAKR 
      DNEVLVQRQ RNGQTVPYR VVDNPAKLS PSDWDRVVA 
      VFVMGPAWQ FKGYPWENP VEIFSKVAA FHIKYDEMK 
      LDANVERWA VTVIELSRT KRHLDRAAL MVFWEKLDR 
      HIMQFKPHL RF
    CDS sequence
    • >TC011263 RA
      atggcggac cctttgagt ttgcttcgc caatataat 
      gtgaataaa aaagaaata atcgaacgc gaaggccag 
      ataatattc ggggaattt tcctggccg aaaaatgtg 
      aaaaccaat tatttaatg tggggttct ggcaaagac 
      ggaacccct aaggaatac tacacgttg gaatgtttg 
      ttgtttatt ttgaaaaat gtatcactg actcatcct 
      gtatatgtg cgacaagcc gctgctgag aatatacca 
      gtagtgaga agacctgac agaaaggaa ctcttggct 
      tatctaaat ggcgaaacg gcgagttgt cctgcgatt 
      gataaaagt gctcctctt gaaataccc acacaggtc 
      aaaagatct gctgactat gaagggcct gaaagtatt 
      gcaaaaaag ccccggttt gaggacttg catgtgcaa 
      aaagttagg gagaagttg gccgcccga ctcgatgcc 
      cctaaagag gcttcagtc actgttgat aatatcaaa 
      tcattgtct gaagccatg tctgtggaa aaaattgca 
      gcgattaaa gccaagcgt ttggcaaag aaacgaacc 
      acaattaaa ggcaatgat gatataggg caagagtac 
      gacattcgg gctattctt gacttggat gtggacttg 
      accaaagac attatcagt cgtgagaga cagtggagg 
      accagaact acaattctg caatctaat ggcaagacc 
      tttgccaag aacattttg gcaatgttg cacagtata 
      aaagcccgc gaggagggc cgtcagcgc cccccacaa 
      caagccccc atcgtcacc ccccgccaa acccccgtc 
      agacccacc acacaaccc gcagtctac aaccgttac 
      gatcaggaa cgtttcaac cgacaaaaa gaagagact 
      gaaggcttc aaaatcgat accatgggc acataccac 
      ggcatgacc cttaaatca gtaactgaa ggtcccgtc 
      aaagcccgc cctcaaccc atccaaccc caaacaccc 
      cttcctgta agcgcagcg cgaccgcct tccggaggc 
      ctcaccaaa agagtctcc cgaacacca attatcata 
      ataccagct gggacaaac agtcttatt tcgatgtac 
      aacgctaaa gaaattcta caagatttg aaattcgtt 
      tcggtggag cagaagaaa aacgagggg gcgaaacga 
      gataatgaa gttctggtt cagagacag agaaatgga 
      caaactgtg ccctatcga gtggtggat aatccggca 
      aagttgagt ccgagtgat tgggatcga gttgtcgct 
      gtgtttgta atggggcct gcgtggcaa ttcaaagga 
      tatccatgg gagaatcca gtcgagatt ttttctaaa 
      gttgctgca tttcatatt aaatatgat gagatgaaa 
      ttggatgca aatgtggag agatgggct gtgactgtt 
      attgaactc tcgaggacg aaaaggcac ttggataga 
      gcagcattg atggtgttt tgggagaaa ctcgatcgg 
      catatcatg cagtttaaa ccccactta cgcttctga
    mRNA sequence
    • >TC011263 RA
      gaatgattg aataacatt gacagtgat taggtgttg 
      catgggttg gtcatggga gctggtgcg agcgaagtt 
      tttcaggtg tcgactggt ggcagcatt gggctcaaa 
      agtagtgca gtaaacaaa aatggcgga ccctttgag 
      tttgcttcg ccaatataa tgtgaataa aaaagaaat 
      aatcgaacg cgaaggcca gataatatt cggggaatt 
      ttcctggcc gaaaaatgt gaaaaccaa ttatttaat 
      gtggggttc tggcaaaga cggaacccc taaggaata 
      ctacacgtt ggaatgttt gttgtttat tttgaaaaa 
      tgtatcact gactcatcc tgtatatgt gcgacaagc 
      cgctgctga gaatatacc agtagtgag aagacctga 
      cagaaagga actcttggc ttatctaaa tggcgaaac 
      ggcgagttg tcctgcgat tgataaaag tgctcctct 
      tgaaatacc cacacaggt caaaagatc tgctgacta 
      tgaagggcc tgaaagtat tgcaaaaaa gccccggtt 
      tgaggactt gcatgtgca aaaagttag ggagaagtt 
      ggccgcccg actcgatgc ccctaaaga ggcttcagt 
      cactgttga taatatcaa atcattgtc tgaagccat 
      gtctgtgga aaaaattgc agcgattaa agccaagcg 
      tttggcaaa gaaacgaac cacaattaa aggcaatga 
      tgatatagg gcaagagta cgacattcg ggctattct 
      tgacttgga tgtggactt gaccaaaga cattatcag 
      tcgtgagag acagtggag gaccagaac tacaattct 
      gcaatctaa tggcaagac ctttgccaa gaacatttt 
      ggcaatgtt gcacagtat aaaagcccg cgaggaggg 
      ccgtcagcg ccccccaca acaagcccc catcgtcac 
      cccccgcca aacccccgt cagacccac cacacaacc 
      cgcagtcta caaccgtta cgatcagga acgtttcaa 
      ccgacaaaa agaagagac tgaaggctt caaaatcga 
      taccatggg cacatacca cggcatgac ccttaaatc 
      agtaactga aggtcccgt caaagcccg ccctcaacc 
      catccaacc ccaaacacc ccttcctgt aagcgcagc 
      gcgaccgcc ttccggagg cctcaccaa aagagtctc 
      ccgaacacc aattatcat aataccagc tgggacaaa 
      cagtcttat ttcgatgta caacgctaa agaaattct 
      acaagattt gaaattcgt ttcggtgga gcagaagaa 
      aaacgaggg ggcgaaacg agataatga agttctggt 
      tcagagaca gagaaatgg acaaactgt gccctatcg 
      agtggtgga taatccggc aaagttgag tccgagtga 
      ttgggatcg agttgtcgc tgtgtttgt aatggggcc 
      tgcgtggca attcaaagg atatccatg ggagaatcc 
      agtcgagat tttttctaa agttgctgc atttcatat 
      taaatatga tgagatgaa attggatgc aaatgtgga 
      gagatgggc tgtgactgt tattgaact ctcgaggac 
      gaaaaggca cttggatag agcagcatt gatggtgtt 
      ttgggagaa actcgatcg gcatatcat gcagtttaa 
      accccactt acgcttctg agtgtgtac agtattgcg 
      ccctgttgt tccaatgtg taattttat caattgatc 
      ttaatcgaa attaggtac tatctacac aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY