Status
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Gene information
General information
TC011257 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 847560..882853
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011257
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05005

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011257 RA
      MANKEIKGA DSVLGLSEI TENKAIWRM 
      LVAEFIGTF LLVVIGCGS IVLSEEVVI 
      VRIGATFGI TVPTLAQSI GHVSGCHIN 
      PAVTLSLFA TGNIKLLRA LLFIAVQLI 
      GAVGGSAVL RLMVPEEKE HSLGITNIG 
      PGITDVQGF LMEIILTFL LLFIIHAVC 
      DPRRKDIKG SAPLAIGLA VTACHLCGI 
      PYSGSSVNP ARSFGPAVI MDSWENHWV 
      CLFVHFSI
    • >TC011257 RB
      MANKEIKGA DSVLGLSEI TENKAIWRM LVAEFIGTF 
      LLVVIGCGS IVLSEEVVI VRIGATFGI TVPTLAQSI 
      GHVSGCHIN PAVTLSLFA TGNIKLLRA LLFIAVQLI 
      GAVGGSAVL RLMVPEEKE HSLGITNIG PGITDVQGF 
      LMEIILTFL LLFIIHAVC DPRRKDIKG SAPLAIGLA 
      VTACHLCGI PYSGSSVNP ARSFGPAVI MDSWENHWV 
      YWAGPLLGG VLAGLIYKY LFKTRKSDV DSYDF
    CDS sequence
    • >TC011257 RA
      atggccaac aaagaaatc aagggtgcg gacagcgtt 
      ttaggcctt tcagaaatc accgaaaac aaagcaata 
      tggcgaatg ttagtggca gaattcatc ggcacattt 
      ttactggtc gtcattggt tgtggctca atagtttta 
      tcagaagag gtagtaatt gttcgcatt ggtgcaaca 
      tttggaata acagttcct acattagca cagagtatt 
      ggtcacgtc agcggctgc cacatcaac cccgcagtg 
      acgctgagc cttttcgca acaggcaac ataaaactt 
      ctccgagct ttgctcttc atcgccgtt caactcatc 
      ggtgctgtt ggaggatca gcagtttta aggttgatg 
      gttcctgaa gaaaaagaa cactccctg ggcatcaca 
      aacataggg ccaggcatc actgacgtc cagggtttc 
      ctgatggaa ataatctta acattttta ttactcttt 
      atcatccac gctgtgtgc gatcctcgc cgtaaagat 
      atcaaagga tcggctcct ctagctata ggattagcc 
      gtcacagct tgccacctc tgtggtatc ccttattcc 
      ggctcaagc gtaaaccca gctcgtagt ttcggaccg 
      gctgtaatc atggattcg tgggaaaat cattgggtg 
      tgtcttttt gtgcatttt tcgatatga
    • >TC011257 RB
      atggccaac aaagaaatc aagggtgcg gacagcgtt 
      ttaggcctt tcagaaatc accgaaaac aaagcaata 
      tggcgaatg ttagtggca gaattcatc ggcacattt 
      ttactggtc gtcattggt tgtggctca atagtttta 
      tcagaagag gtagtaatt gttcgcatt ggtgcaaca 
      tttggaata acagttcct acattagca cagagtatt 
      ggtcacgtc agcggctgc cacatcaac cccgcagtg 
      acgctgagc cttttcgca acaggcaac ataaaactt 
      ctccgagct ttgctcttc atcgccgtt caactcatc 
      ggtgctgtt ggaggatca gcagtttta aggttgatg 
      gttcctgaa gaaaaagaa cactccctg ggcatcaca 
      aacataggg ccaggcatc actgacgtc cagggtttc 
      ctgatggaa ataatctta acattttta ttactcttt 
      atcatccac gctgtgtgc gatcctcgc cgtaaagat 
      atcaaagga tcggctcct ctagctata ggattagcc 
      gtcacagct tgccacctc tgtggtatc ccttattcc 
      ggctcaagc gtaaaccca gctcgtagt ttcggaccg 
      gctgtaatc atggattcg tgggaaaat cattgggtc 
      tactgggct gggccgcta cttggcggt gtgttggct 
      ggtctcatc tacaaatat ctcttcaaa acgagaaaa 
      tctgacgtc gattcgtac gatttttaa
    mRNA sequence
    • >TC011257 RA
      attcagttc cactcctga ccgacattc aaatctctt 
      gacttacga gagattttg tatgattga cactgcact 
      ctcaatcta gtttagcgg tggttcgtt acacatact 
      gtgaatagt tgcgataag cggtgctct cgtttcagc 
      agtttcagt ttcagtgca ccagccgcc gaaatggcc 
      aacaaagaa atcaagggt gcggacagc gttttaggc 
      ctttcagaa atcaccgaa aacaaagca atatggcga 
      atgttagtg gcagaattc atcggcaca tttttactg 
      gtcgtcatt ggttgtggc tcaatagtt ttatcagaa 
      gaggtagta attgttcgc attggtgca acatttgga 
      ataacagtt cctacatta gcacagagt attggtcac 
      gtcagcggc tgccacatc aaccccgca gtgacgctg 
      agccttttc gcaacaggc aacataaaa cttctccga 
      gctttgctc ttcatcgcc gttcaactc atcggtgct 
      gttggagga tcagcagtt ttaaggttg atggttcct 
      gaagaaaaa gaacactcc ctgggcatc acaaacata 
      gggccaggc atcactgac gtccagggt ttcctgatg 
      gaaataatc ttaacattt ttattactc tttatcatc 
      cacgctgtg tgcgatcct cgccgtaaa gatatcaaa 
      ggatcggct cctctagct ataggatta gccgtcaca 
      gcttgccac ctctgtggt atcccttat tccggctca 
      agcgtaaac ccagctcgt agtttcgga ccggctgta 
      atcatggat tcgtgggaa aatcattgg gtgtgtctt 
      tttgtgcat ttttcgata tgagtttga cccatttta 
      acaaaggac agttgattg cattgcaac tttcatgta 
      aaatggtta aaagtgctt aacacgatt tcccatgat 
      gcattttca ttcattcat ctttttatt aacaattaa 
      ttagttgta gtcatgtta ttaatgtta gtgtgtcga 
      ctcttactt tattttctt cttgtttca ggtctactg 
      ggctgggcc gctacttgg cggtgtgtt ggctggtct 
      catctacaa atatctctt caaaacgag aaaatctga 
      cgtcgattc gtacgattt ttaagcgaa aatctttca 
      tgtttcaca attttgtca ttcatttca gctaactcc 
      atttgcttc gtagttacg tactaattt atgtacaat 
      ttttataat actttttaa aaaagtagt taagtacgt 
      tttaagtgt atagtagtg ttagtttta ttgataagt 
      ccaaattgt ttcaatttt ggaaaataa atgaaaaac 
      tatcccaat ttcaaaatc ttctgccat atggaggct 
      cttctactc taaggtttc taattgtac aataccaaa 
      ttatttata aggaactat gaatccatt ctttaataa 
      agtgttcga aaattt
    • >TC011257 RB
      attcagttc cactcctga ccgacattc aaatctctt 
      gacttacga gagattttg tatgattga cactgcact 
      ctcaatcta gtttagcgg tggttcgtt acacatact 
      gtgaatagt tgcgataag cggtgctct cgtttcagc 
      agtttcagt ttcagtgca ccagccgcc gaaatggcc 
      aacaaagaa atcaagggt gcggacagc gttttaggc 
      ctttcagaa atcaccgaa aacaaagca atatggcga 
      atgttagtg gcagaattc atcggcaca tttttactg 
      gtcgtcatt ggttgtggc tcaatagtt ttatcagaa 
      gaggtagta attgttcgc attggtgca acatttgga 
      ataacagtt cctacatta gcacagagt attggtcac 
      gtcagcggc tgccacatc aaccccgca gtgacgctg 
      agccttttc gcaacaggc aacataaaa cttctccga 
      gctttgctc ttcatcgcc gttcaactc atcggtgct 
      gttggagga tcagcagtt ttaaggttg atggttcct 
      gaagaaaaa gaacactcc ctgggcatc acaaacata 
      gggccaggc atcactgac gtccagggt ttcctgatg 
      gaaataatc ttaacattt ttattactc tttatcatc 
      cacgctgtg tgcgatcct cgccgtaaa gatatcaaa 
      ggatcggct cctctagct ataggatta gccgtcaca 
      gcttgccac ctctgtggt atcccttat tccggctca 
      agcgtaaac ccagctcgt agtttcgga ccggctgta 
      atcatggat tcgtgggaa aatcattgg gtctactgg 
      gctgggccg ctacttggc ggtgtgttg gctggtctc 
      atctacaaa tatctcttc aaaacgaga aaatctgac 
      gtcgattcg tacgatttt taagcgaaa atctttcat 
      gtttcacaa ttttgtcat tcatttcag ctaactcca 
      tttgcttcg tagttacgt actaattta tgtacaatt 
      tttataata ctttttaaa aaagtagtt aagtacgtt 
      ttaagtgta tagtagtgt tagttttat tgataagtc 
      caaattgtt tcaattttg gaaaataaa tgaaaaact 
      atcccaatt tcaaaatct tctgccata tggaggctc 
      ttctactct aaggtttct aattgtaca ataccaaat 
      tatttataa ggaactatg aatccattc tttaataaa 
      gtgttcgaa aattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY