| General information | |||
| TC011252 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007425.3: 677987..695853 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC011252 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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|---|---|---|
| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
For help on how to submit larger datasets or non experimental data please contact us.
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05004 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)Days post injection
Fertility
mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: -
Number of animals showing the phenotype: 4
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 9 (11 dpi)
Days post injection
- >TC011252 RA
MNVTRIWTR GRPHSNEFV MEYTISYGT NGLDYADYK EPSGNAMMF TGNSDGDSI HKNEFEVPI IAQWIRINP TRWRDRISM RVELYGCDY EADRMYFNG TALLKLDMM RDPVSASRE SIRFRFKTA VANGVVLYS RGTQGDYIA LELRDNRML LKIDLGSGI VTSLCVGSL LDDNIWHDV VISRNRRDI LFSVDRVVV QDKIKGEFN RLNLNQAFY VGGVPNIQE GLEVVQNFT GCIENIYLN ATNLIRDLK HANMYNEPA LRFERVNTL YNCPEPPII PVTFLTKNS FAKLKGYEG MSSLNASFA FRTYEKDGL MFFHAFSTS GHVAIFLEE GKIKVELIT SGVSKVVLD NYKEKFNDG RWHSVVLTI STNLLIFNV DYRPMKTVR LLTIKTGGT YLVAGGVTT SLGYSGQPM PGFIGCMRT IGIDGNYRL PTDWTKGEY CCEDQVVFD ACRMTDRCN PNPCQHGGL CKQNALEFF CDCKNTGYG GAVCHTSIN PLSCQAYKN VQAVNHKAE IKIDVDGSG PLGPFPVTC EFYSDGRVA TVLHHSNQE TTPVDGFQE PGSFRQDIH YEADDDQVA ALINRSSTC RQHLQYACR GARLFNSPT DETRFSPFS WWVSRHNQK MDYWGGALP GSRKCACGV SGTCVDPTK WCNCDSSND YNADKWLVD AGDITEKEN LPVKQLRFG DTGTALDYK EGRYTLGPL LCEGDDLFN NVITFRISD ATINLPTFD MGHSGDIYF EFRTAVENA VLFHSKGPS DFIKLSIVG GNQLQFQYQ AGLGPMAVI RETSYKLSD DRWHSVSVE RNRKAAMII VDGALKAEV REPPGPVRA LHLTSELVI GASTDYRDG FTGCIRALL INGQQVDLR SYARRGLYG VTEGCIGKC QSNPCLNNG TCFERYDSY WCDCRWTAF KGPICADEI GVNMRQTSM VKYDFMGSW RSTIAENIR VGFTTTNPK GFLLGFSSN ISKEYLTIM VSNSGHLRV VFDFGFERQ EVIYPEKLF SLGQYHDFR LSRKNSGAT LVLQVDNNK PVEYHFNIK ASADAQFNN IQYMYIGRN ESMTEGFIG CISRVEFDD IYPLKLLFQ QSGPINVQS LGTPLTEDY CGVEPITHP PDLIETRPP PIVDEEQLR KAYNQTDSA ILGSVLAII FLALVILAV IIGRYLHRH KGEYLTQED AGADTALDP DTAVVHGTT GHHVQKKKE WFI
- >TC011252 RB
MDLGGPFEI RSISTQGRK GSNEHVTEY IIQFSDDGE AWRSFTDAQ GEIEMFTGN SDGDSIHKN EFEVPIIAQ WIRINPTRW RDRISMRVE LYGCDYEAD RMYFNGTAL LKLDMMRDP VSASRESIR FRFKTAVAN GVVLYSRGT QGDYIALEL RDNRMLLKI DLGSGIVTS LCVGSLLDD NIWHDVVIS RNRRDILFS VDRVVVQDK IKGEFNRLN LNQAFYVGG VPNIQEGLE VVQNFTGCI ENIYLNATN LIRDLKHAN MYNEPALRF ERVNTLYNC PEPPIIPVT FLTKNSFAK LKGYEGMSS LNASFAFRT YEKDGLMFF HAFSTSGHV AIFLEEGKI KVELITSGV SKVVLDNYK EKFNDGRWH SVVLTISTN LLIFNVDYR PMKTVRLLT IKTGGTYLV AGGVTTSLG YSGQPMPGF IGCMRTIGI DGNYRLPTD WTKGEYCCE DQVVFDACR MTDRCNPNP CQHGGLCKQ NALEFFCDC KNTGYGGAV CHTSINPLS CQAYKNVQA VNHKAEIKI DVDGSGPLG PFPVTCEFY SDGRVATVL HHSNQETTP VDGFQEPGS FRQDIHYEA DDDQVAALI NRSSTCRQH LQYACRGAR LFNSPTDET RFSPFSWWV SRHNQKMDY WGGALPGSR KCACGVSGT CVDPTKWCN CDSSNDYNA DKWLVDAGD ITEKENLPV KQLRFGDTG TALDYKEGR YTLGPLLCE GDDLFNNVI TFRISDATI NLPTFDMGH SGDIYFEFR TAVENAVLF HSKGPSDFI KLSIVGGNQ LQFQYQAGL GPMAVIRET SYKLSDDRW HSVSVERNR KAAMIIVDG ALKAEVREP PGPVRALHL TSELVIGAS TDYRDGFTG CIRALLING QQVDLRSYA RRGLYGVTE GCIGKCQSN PCLNNGTCF ERYDSYWCD CRWTAFKGP ICADEIGVN MRQTSMVKY DFMGSWRST IAENIRVGF TTTNPKGFL LGFSSNISK EYLTIMVSN SGHLRVVFD FGFERQEVI YPEKLFSLG QYHDFRLSR KNSGATLVL QVDNNKPVE YHFNIKASA DAQFNNIQY MYIGRNESM TEGFIGCIS RVEFDDIYP LKLLFQQSG PINVQSLGT PLTEDYCGV EPITHPPDL IETRPPPIV DEEQLRKAY NQTDSAILG SVLAIIFLA LVILAVIIG RYLHRHKGE YLTQEDAGA DTALDPDTA VVHGTTGHH VQKKKEWFI
- >TC011252 RA
atgaacgta acgaggatc tggacccgt ggtagacct cacagcaat gagtttgta atggaatac actattagt tatggtact aacggttta gattatgca gactacaaa gaacctagt ggtaatgca atgatgttt acgggtaat tcagacggt gactcgatt cacaaaaac gaattcgaa gtgcccata atcgcgcaa tggattcgc ataaatccg accagatgg cgcgaccgt atctcgatg agagtggag ctctacggt tgtgattat gaggctgat cgtatgtat ttcaacgga accgctttg ctcaagctc gatatgatg cgagatccc gtgtcggca tctcgtgag agtatccga tttaggttt aagacggct gtggcgaat ggagttgtg ttgtacagt aggggaaca caaggagat tacattgct ttggagtta cgagacaat aggatgttg cttaagatc gatttagga tcgggaatt gtgactagt ttatgtgtc ggaagtttg ctagacgat aatatttgg cacgacgtt gttatttca aggaatcga agggatatt ttgttctcg gttgataga gtggtggtt caggataag attaaaggg gagttcaac agactcaac ttgaaccaa gcgttttac gttggtgga gttcctaat attcaagag ggtttagaa gtggtccaa aattttacc ggttgcatt gaaaatatt tacttaaat gcaacaaat ctcattcgt gatttaaaa cacgcgaat atgtataat gagccggcg ttgcgtttt gaaagagtc aacactttg tacaactgt ccggaacct ccaattatt ccagtcact ttcctgacg aagaattct tttgcaaaa cttaagggt tatgaagga atgagttcg ttgaatgct tcgtttgcc ttcagaacg tacgaaaag gacggattg atgttcttc catgcgttt tcaacgtct ggacatgtt gcgatattc ctagaagag ggaaaaatc aaagtcgag ctaataacc tccggcgtt tcgaaagta gttctcgac aactacaag gagaaattc aacgacggt cgatggcac tctgtcgtc ttaacaatc agcaccaac ttactaatc ttcaacgtg gactaccgc cctatgaaa accgtccgc ctcctaacg atcaaaacc ggcggtact tacctagta gcaggcggt gtaaccaca tctctcggt tactcgggc caaccgatg cccggtttc atcggttgc atgcgcaca atcggcatc gacggcaac taccgccta ccaaccgat tggacaaaa ggcgaatac tgttgcgaa gatcaagtc gtctttgac gcttgccga atgaccgat cgctgcaac cccaaccca tgccaacac ggcgggctg tgcaaacaa aacgcgctg gaattcttc tgcgattgc aaaaacaca ggttatggc ggcgccgtt tgtcacact tcaatcaat ccactgtcg tgtcaagcc tacaagaac gtccaagcg gtcaatcac aaagccgag attaaaatc gacgttgat gggagcggg ccgttgggc ccgtttccc gtaacgtgc gagttttat tccgatggt cgcgttgcc actgtctta caccacagt aaccaggag accacgcca gttgatggg ttccaagaa ccggggagt tttcgacag gacatacac tatgaagcg gatgatgat caagtggcc gcgttgatt aaccggtca agtacgtgt cgacaacac ttgcagtac gcgtgtcgt ggcgctcgt ttgtttaat tcacccacg gatgagact agatttagt ccgttttcg tggtgggtg tccagacac aaccagaag atggattat tggggcggt gccttgcct ggatcacgt aaatgtgcg tgtggtgtt tctggcact tgtgtggat ccaaccaag tggtgtaat tgtgacagt agtaatgac tataacgct gataagtgg ctagtcgat gctggggat ataaccgaa aaggagaat ctcccagtg aaacaattg cggttcggt gacaccgga accgccctc gattataaa gaaggtcgc tacactttg gggccgttg ttgtgcgaa ggcgatgat ttgtttaat aacgtgatc acgttccga atatcagac gctactatc aatttgcca acttttgac atgggtcat agtggtgat atatatttt gagttccga acagctgtg gaaaacgct gttttgttc cattcaaaa ggcccgtct gactttatc aagttgagt atagttggt ggtaaccaa ctacaattc caatatcaa gccggcttg ggccccatg gctgtgatc cgcgaaacg tcatataaa ctctcggac gatagatgg cactcagtc tcagtcgaa cgtaacagg aaggcggcc atgatcatt gttgacggt gctttgaag gccgaagtg agggaaccc ccgggtccc gtgcgcgcc ctccacctg acttcggaa cttgtgatc ggggcgtcc acggactac cgcgatggc ttcaccggg tgcattcgc gccctttta atcaacgga caacaagtc gatttgaga agttacgcc cgtcggggg ctatacggc gtaaccgaa ggttgtatc ggcaagtgt caaagcaac ccatgcctc aataacggc acttgtttc gagcgctat gacagttac tggtgtgat tgtcgttgg acggcgttc aaagggccg atttgtgcc gacgaaatc ggtgttaac atgcgtcag acgtccatg gttaagtac gattttatg gggtcgtgg cgttcgacc attgccgaa aatatccga gtcgggttc acgacgacg aatcccaaa ggtttcctt ttgggtttc tcgtcgaat atctcgaag gagtatttg actattatg gtgtcgaat tcgggacat ttgcgtgtg gttttcgat tttgggttc gaacggcag gaggtgatc tatccggag aagctgttc agtttgggg cagtatcac gattttcga ctctcgcgg aagaattcg ggggccacg ttggtgctt caggttgat aataataaa ccggttgag tatcacttt aatattaag gcgtcggcc gatgcacag ttcaataac atacagtat atgtatatt ggacggaat gagtcgatg actgagggt ttcattggt tgtatttcg cgggtcgag tttgatgat atttatcca ttgaagttg ttgttccaa cagtcgggg cctattaac gtccagtcg ttaggaact ccgttgact gaggattat tgtggggtg gaacccata actcatccg cccgatctc attgaaacg agaccaccg cccatcgtt gatgaggag cagttgagg aaggcgtac aatcaaacg gattcagct attttagga agtgttttg gcgattatt ttcctagct ttggtgatc ctggccgta ataattggg cgatatttg caccgtcac aaaggcgaa tatttgacg caggaggac gcaggagcc gacactgca ctcgaccca gacaccgct gttgtacac ggaacaaca ggacatcac gtccagaaa aagaaggag tggttcata tag
- >TC011252 RB
atggattta gggggtcct tttgagatc cgctcgatt agtacgcaa ggtcggaaa gggtccaac gagcacgtt accgaatat attatacag ttttctgac gacggggag gcttggagg agctttact gatgctcaa ggagaaatc gagatgttt acgggtaat tcagacggt gactcgatt cacaaaaac gaattcgaa gtgcccata atcgcgcaa tggattcgc ataaatccg accagatgg cgcgaccgt atctcgatg agagtggag ctctacggt tgtgattat gaggctgat cgtatgtat ttcaacgga accgctttg ctcaagctc gatatgatg cgagatccc gtgtcggca tctcgtgag agtatccga tttaggttt aagacggct gtggcgaat ggagttgtg ttgtacagt aggggaaca caaggagat tacattgct ttggagtta cgagacaat aggatgttg cttaagatc gatttagga tcgggaatt gtgactagt ttatgtgtc ggaagtttg ctagacgat aatatttgg cacgacgtt gttatttca aggaatcga agggatatt ttgttctcg gttgataga gtggtggtt caggataag attaaaggg gagttcaac agactcaac ttgaaccaa gcgttttac gttggtgga gttcctaat attcaagag ggtttagaa gtggtccaa aattttacc ggttgcatt gaaaatatt tacttaaat gcaacaaat ctcattcgt gatttaaaa cacgcgaat atgtataat gagccggcg ttgcgtttt gaaagagtc aacactttg tacaactgt ccggaacct ccaattatt ccagtcact ttcctgacg aagaattct tttgcaaaa cttaagggt tatgaagga atgagttcg ttgaatgct tcgtttgcc ttcagaacg tacgaaaag gacggattg atgttcttc catgcgttt tcaacgtct ggacatgtt gcgatattc ctagaagag ggaaaaatc aaagtcgag ctaataacc tccggcgtt tcgaaagta gttctcgac aactacaag gagaaattc aacgacggt cgatggcac tctgtcgtc ttaacaatc agcaccaac ttactaatc ttcaacgtg gactaccgc cctatgaaa accgtccgc ctcctaacg atcaaaacc ggcggtact tacctagta gcaggcggt gtaaccaca tctctcggt tactcgggc caaccgatg cccggtttc atcggttgc atgcgcaca atcggcatc gacggcaac taccgccta ccaaccgat tggacaaaa ggcgaatac tgttgcgaa gatcaagtc gtctttgac gcttgccga atgaccgat cgctgcaac cccaaccca tgccaacac ggcgggctg tgcaaacaa aacgcgctg gaattcttc tgcgattgc aaaaacaca ggttatggc ggcgccgtt tgtcacact tcaatcaat ccactgtcg tgtcaagcc tacaagaac gtccaagcg gtcaatcac aaagccgag attaaaatc gacgttgat gggagcggg ccgttgggc ccgtttccc gtaacgtgc gagttttat tccgatggt cgcgttgcc actgtctta caccacagt aaccaggag accacgcca gttgatggg ttccaagaa ccggggagt tttcgacag gacatacac tatgaagcg gatgatgat caagtggcc gcgttgatt aaccggtca agtacgtgt cgacaacac ttgcagtac gcgtgtcgt ggcgctcgt ttgtttaat tcacccacg gatgagact agatttagt ccgttttcg tggtgggtg tccagacac aaccagaag atggattat tggggcggt gccttgcct ggatcacgt aaatgtgcg tgtggtgtt tctggcact tgtgtggat ccaaccaag tggtgtaat tgtgacagt agtaatgac tataacgct gataagtgg ctagtcgat gctggggat ataaccgaa aaggagaat ctcccagtg aaacaattg cggttcggt gacaccgga accgccctc gattataaa gaaggtcgc tacactttg gggccgttg ttgtgcgaa ggcgatgat ttgtttaat aacgtgatc acgttccga atatcagac gctactatc aatttgcca acttttgac atgggtcat agtggtgat atatatttt gagttccga acagctgtg gaaaacgct gttttgttc cattcaaaa ggcccgtct gactttatc aagttgagt atagttggt ggtaaccaa ctacaattc caatatcaa gccggcttg ggccccatg gctgtgatc cgcgaaacg tcatataaa ctctcggac gatagatgg cactcagtc tcagtcgaa cgtaacagg aaggcggcc atgatcatt gttgacggt gctttgaag gccgaagtg agggaaccc ccgggtccc gtgcgcgcc ctccacctg acttcggaa cttgtgatc ggggcgtcc acggactac cgcgatggc ttcaccggg tgcattcgc gccctttta atcaacgga caacaagtc gatttgaga agttacgcc cgtcggggg ctatacggc gtaaccgaa ggttgtatc ggcaagtgt caaagcaac ccatgcctc aataacggc acttgtttc gagcgctat gacagttac tggtgtgat tgtcgttgg acggcgttc aaagggccg atttgtgcc gacgaaatc ggtgttaac atgcgtcag acgtccatg gttaagtac gattttatg gggtcgtgg cgttcgacc attgccgaa aatatccga gtcgggttc acgacgacg aatcccaaa ggtttcctt ttgggtttc tcgtcgaat atctcgaag gagtatttg actattatg gtgtcgaat tcgggacat ttgcgtgtg gttttcgat tttgggttc gaacggcag gaggtgatc tatccggag aagctgttc agtttgggg cagtatcac gattttcga ctctcgcgg aagaattcg ggggccacg ttggtgctt caggttgat aataataaa ccggttgag tatcacttt aatattaag gcgtcggcc gatgcacag ttcaataac atacagtat atgtatatt ggacggaat gagtcgatg actgagggt ttcattggt tgtatttcg cgggtcgag tttgatgat atttatcca ttgaagttg ttgttccaa cagtcgggg cctattaac gtccagtcg ttaggaact ccgttgact gaggattat tgtggggtg gaacccata actcatccg cccgatctc attgaaacg agaccaccg cccatcgtt gatgaggag cagttgagg aaggcgtac aatcaaacg gattcagct attttagga agtgttttg gcgattatt ttcctagct ttggtgatc ctggccgta ataattggg cgatatttg caccgtcac aaaggcgaa tatttgacg caggaggac gcaggagcc gacactgca ctcgaccca gacaccgct gttgtacac ggaacaaca ggacatcac gtccagaaa aagaaggag tggttcata tag
- >TC011252 RA
acgaaggtg ggaacgcat ggagtgccg aagaaaacg attttgacc aacacctaa tcgttgatt tgggcgaag taatgaacg taacgagga tctggaccc gtggtagac ctcacagca atgagtttg taatggaat acactatta gttatggta ctaacggtt tagattatg cagactaca aagaaccta gtggtaatg caatgatgt ttacgggta attcagacg gtgactcga ttcacaaaa acgaattcg aagtgccca taatcgcgc aatggattc gcataaatc cgaccagat ggcgcgacc gtatctcga tgagagtgg agctctacg gttgtgatt atgaggctg atcgtatgt atttcaacg gaaccgctt tgctcaagc tcgatatga tgcgagatc ccgtgtcgg catctcgtg agagtatcc gatttaggt ttaagacgg ctgtggcga atggagttg tgttgtaca gtaggggaa cacaaggag attacattg ctttggagt tacgagaca ataggatgt tgcttaaga tcgatttag gatcgggaa ttgtgacta gtttatgtg tcggaagtt tgctagacg ataatattt ggcacgacg ttgttattt caaggaatc gaagggata ttttgttct cggttgata gagtggtgg ttcaggata agattaaag gggagttca acagactca acttgaacc aagcgtttt acgttggtg gagttccta atattcaag agggtttag aagtggtcc aaaatttta ccggttgca ttgaaaata tttacttaa atgcaacaa atctcattc gtgatttaa aacacgcga atatgtata atgagccgg cgttgcgtt ttgaaagag tcaacactt tgtacaact gtccggaac ctccaatta ttccagtca ctttcctga cgaagaatt cttttgcaa aacttaagg gttatgaag gaatgagtt cgttgaatg cttcgtttg ccttcagaa cgtacgaaa aggacggat tgatgttct tccatgcgt tttcaacgt ctggacatg ttgcgatat tcctagaag agggaaaaa tcaaagtcg agctaataa cctccggcg tttcgaaag tagttctcg acaactaca aggagaaat tcaacgacg gtcgatggc actctgtcg tcttaacaa tcagcacca acttactaa tcttcaacg tggactacc gccctatga aaaccgtcc gcctcctaa cgatcaaaa ccggcggta cttacctag tagcaggcg gtgtaacca catctctcg gttactcgg gccaaccga tgcccggtt tcatcggtt gcatgcgca caatcggca tcgacggca actaccgcc taccaaccg attggacaa aaggcgaat actgttgcg aagatcaag tcgtctttg acgcttgcc gaatgaccg atcgctgca accccaacc catgccaac acggcgggc tgtgcaaac aaaacgcgc tggaattct tctgcgatt gcaaaaaca caggttatg gcggcgccg tttgtcaca cttcaatca atccactgt cgtgtcaag cctacaaga acgtccaag cggtcaatc acaaagccg agattaaaa tcgacgttg atgggagcg ggccgttgg gcccgtttc ccgtaacgt gcgagtttt attccgatg gtcgcgttg ccactgtct tacaccaca gtaaccagg agaccacgc cagttgatg ggttccaag aaccgggga gttttcgac aggacatac actatgaag cggatgatg atcaagtgg ccgcgttga ttaaccggt caagtacgt gtcgacaac acttgcagt acgcgtgtc gtggcgctc gtttgttta attcaccca cggatgaga ctagattta gtccgtttt cgtggtggg tgtccagac acaaccaga agatggatt attggggcg gtgccttgc ctggatcac gtaaatgtg cgtgtggtg tttctggca cttgtgtgg atccaacca agtggtgta attgtgaca gtagtaatg actataacg ctgataagt ggctagtcg atgctgggg atataaccg aaaaggaga atctcccag tgaaacaat tgcggttcg gtgacaccg gaaccgccc tcgattata aagaaggtc gctacactt tggggccgt tgttgtgcg aaggcgatg atttgttta ataacgtga tcacgttcc gaatatcag acgctacta tcaatttgc caacttttg acatgggtc atagtggtg atatatatt ttgagttcc gaacagctg tggaaaacg ctgttttgt tccattcaa aaggcccgt ctgacttta tcaagttga gtatagttg gtggtaacc aactacaat tccaatatc aagccggct tgggcccca tggctgtga tccgcgaaa cgtcatata aactctcgg acgatagat ggcactcag tctcagtcg aacgtaaca ggaaggcgg ccatgatca ttgttgacg gtgctttga aggccgaag tgagggaac ccccgggtc ccgtgcgcg ccctccacc tgacttcgg aacttgtga tcggggcgt ccacggact accgcgatg gcttcaccg ggtgcattc gcgcccttt taatcaacg gacaacaag tcgatttga gaagttacg cccgtcggg ggctatacg gcgtaaccg aaggttgta tcggcaagt gtcaaagca acccatgcc tcaataacg gcacttgtt tcgagcgct atgacagtt actggtgtg attgtcgtt ggacggcgt tcaaagggc cgatttgtg ccgacgaaa tcggtgtta acatgcgtc agacgtcca tggttaagt acgatttta tggggtcgt ggcgttcga ccattgccg aaaatatcc gagtcgggt tcacgacga cgaatccca aaggtttcc ttttgggtt tctcgtcga atatctcga aggagtatt tgactatta tggtgtcga attcgggac atttgcgtg tggttttcg attttgggt tcgaacggc aggaggtga tctatccgg agaagctgt tcagtttgg ggcagtatc acgattttc gactctcgc ggaagaatt cgggggcca cgttggtgc ttcaggttg ataataata aaccggttg agtatcact ttaatatta aggcgtcgg ccgatgcac agttcaata acatacagt atatgtata ttggacgga atgagtcga tgactgagg gtttcattg gttgtattt cgcgggtcg agtttgatg atatttatc cattgaagt tgttgttcc aacagtcgg ggcctatta acgtccagt cgttaggaa ctccgttga ctgaggatt attgtgggg tggaaccca taactcatc cgcccgatc tcattgaaa cgagaccac cgcccatcg ttgatgagg agcagttga ggaaggcgt acaatcaaa cggattcag ctattttag gaagtgttt tggcgatta ttttcctag ctttggtga tcctggccg taataattg ggcgatatt tgcaccgtc acaaaggcg aatatttga cgcaggagg acgcaggag ccgacactg cactcgacc cagacaccg ctgttgtac acggaacaa caggacatc acgtccaga aaaagaagg agtggttca tatagtcga aaattcgtt aaattaatt atgggccta atagtacaa caagattgt tttaaataa ggcagtttt cacggttgt gaaaattta tttacattg aaagaccgt ttatttttt tattgtttt tatttagga tgtgttcat gtggcataa acttttgtt ttgtgcaat ttctgtact tttttgact tagtttctt tccgtccat tttatttta ggagtcgca gactccgcg tatttaaca acattccag tattattta tggttctaa aacactaaa attcgatca cttgatctt ccaaaaggt tagtcgcta gaataagtg cctttttac ggcatgtca tcgatactt tgcactgta acttatctt ttgttgctt ttttaaatt gtattatat tgtaatact gactagtat gagatattg tgtaatttt ttacaaata aaaatacat aaaacac
- >TC011252 RB
acgaaggtg ggaacgcat ggagtgccg aagaaaacg attttgacc aacacctaa tcgttgatt tgggcgaag taatgaacg taacgagga tctggaccc gtggtagac ctcacagca atgagtttg taatggaat acactatta gttatggta ctaacggtt tagattatg cagactaca aagaaccta gtggtaatg caatggtga gatattacc catcttttt ttttctcgt taatcatgg gtatcagtc tacgtttta ctaatcctg catgtttgt ggctgtcgt agttgtgct aattttgcc tactaacat tctgcaatc tattaacac taaacactt ggctatagg atcgtccgc ttggagtcc ggaagaaag cacatattt tcactttct aacaatgga tttaggggg tccttttga gatccgctc gattagtac gcaaggtcg gaaagggtc caacgagca cgttaccga atatattat acagttttc tgacgacgg ggaggcttg gaggagctt tactgatgc tcaaggaga aatcgagat gtttacggg taattcaga cggtgactc gattcacaa aaacgaatt cgaagtgcc cataatcgc gcaatggat tcgcataaa tccgaccag atggcgcga ccgtatctc gatgagagt ggagctcta cggttgtga ttatgaggc tgatcgtat gtatttcaa cggaaccgc tttgctcaa gctcgatat gatgcgaga tcccgtgtc ggcatctcg tgagagtat ccgatttag gtttaagac ggctgtggc gaatggagt tgtgttgta cagtagggg aacacaagg agattacat tgctttgga gttacgaga caataggat gttgcttaa gatcgattt aggatcggg aattgtgac tagtttatg tgtcggaag tttgctaga cgataatat ttggcacga cgttgttat ttcaaggaa tcgaaggga tattttgtt ctcggttga tagagtggt ggttcagga taagattaa aggggagtt caacagact caacttgaa ccaagcgtt ttacgttgg tggagttcc taatattca agagggttt agaagtggt ccaaaattt taccggttg cattgaaaa tatttactt aaatgcaac aaatctcat tcgtgattt aaaacacgc gaatatgta taatgagcc ggcgttgcg ttttgaaag agtcaacac tttgtacaa ctgtccgga acctccaat tattccagt cactttcct gacgaagaa ttcttttgc aaaacttaa gggttatga aggaatgag ttcgttgaa tgcttcgtt tgccttcag aacgtacga aaaggacgg attgatgtt cttccatgc gttttcaac gtctggaca tgttgcgat attcctaga agagggaaa aatcaaagt cgagctaat aacctccgg cgtttcgaa agtagttct cgacaacta caaggagaa attcaacga cggtcgatg gcactctgt cgtcttaac aatcagcac caacttact aatcttcaa cgtggacta ccgccctat gaaaaccgt ccgcctcct aacgatcaa aaccggcgg tacttacct agtagcagg cggtgtaac cacatctct cggttactc gggccaacc gatgcccgg tttcatcgg ttgcatgcg cacaatcgg catcgacgg caactaccg cctaccaac cgattggac aaaaggcga atactgttg cgaagatca agtcgtctt tgacgcttg ccgaatgac cgatcgctg caaccccaa cccatgcca acacggcgg gctgtgcaa acaaaacgc gctggaatt cttctgcga ttgcaaaaa cacaggtta tggcggcgc cgtttgtca cacttcaat caatccact gtcgtgtca agcctacaa gaacgtcca agcggtcaa tcacaaagc cgagattaa aatcgacgt tgatgggag cgggccgtt gggcccgtt tcccgtaac gtgcgagtt ttattccga tggtcgcgt tgccactgt cttacacca cagtaacca ggagaccac gccagttga tgggttcca agaaccggg gagttttcg acaggacat acactatga agcggatga tgatcaagt ggccgcgtt gattaaccg gtcaagtac gtgtcgaca acacttgca gtacgcgtg tcgtggcgc tcgtttgtt taattcacc cacggatga gactagatt tagtccgtt ttcgtggtg ggtgtccag acacaacca gaagatgga ttattgggg cggtgcctt gcctggatc acgtaaatg tgcgtgtgg tgtttctgg cacttgtgt ggatccaac caagtggtg taattgtga cagtagtaa tgactataa cgctgataa gtggctagt cgatgctgg ggatataac cgaaaagga gaatctccc agtgaaaca attgcggtt cggtgacac cggaaccgc cctcgatta taaagaagg tcgctacac tttggggcc gttgttgtg cgaaggcga tgatttgtt taataacgt gatcacgtt ccgaatatc agacgctac tatcaattt gccaacttt tgacatggg tcatagtgg tgatatata ttttgagtt ccgaacagc tgtggaaaa cgctgtttt gttccattc aaaaggccc gtctgactt tatcaagtt gagtatagt tggtggtaa ccaactaca attccaata tcaagccgg cttgggccc catggctgt gatccgcga aacgtcata taaactctc ggacgatag atggcactc agtctcagt cgaacgtaa caggaaggc ggccatgat cattgttga cggtgcttt gaaggccga agtgaggga acccccggg tcccgtgcg cgccctcca cctgacttc ggaacttgt gatcggggc gtccacgga ctaccgcga tggcttcac cgggtgcat tcgcgccct tttaatcaa cggacaaca agtcgattt gagaagtta cgcccgtcg ggggctata cggcgtaac cgaaggttg tatcggcaa gtgtcaaag caacccatg cctcaataa cggcacttg tttcgagcg ctatgacag ttactggtg tgattgtcg ttggacggc gttcaaagg gccgatttg tgccgacga aatcggtgt taacatgcg tcagacgtc catggttaa gtacgattt tatggggtc gtggcgttc gaccattgc cgaaaatat ccgagtcgg gttcacgac gacgaatcc caaaggttt ccttttggg tttctcgtc gaatatctc gaaggagta tttgactat tatggtgtc gaattcggg acatttgcg tgtggtttt cgattttgg gttcgaacg gcaggaggt gatctatcc ggagaagct gttcagttt ggggcagta tcacgattt tcgactctc gcggaagaa ttcgggggc cacgttggt gcttcaggt tgataataa taaaccggt tgagtatca ctttaatat taaggcgtc ggccgatgc acagttcaa taacataca gtatatgta tattggacg gaatgagtc gatgactga gggtttcat tggttgtat ttcgcgggt cgagtttga tgatattta tccattgaa gttgttgtt ccaacagtc ggggcctat taacgtcca gtcgttagg aactccgtt gactgagga ttattgtgg ggtggaacc cataactca tccgcccga tctcattga aacgagacc accgcccat cgttgatga ggagcagtt gaggaaggc gtacaatca aacggattc agctatttt aggaagtgt tttggcgat tattttcct agctttggt gatcctggc cgtaataat tgggcgata tttgcaccg tcacaaagg cgaatattt gacgcagga ggacgcagg agccgacac tgcactcga cccagacac cgctgttgt acacggaac aacaggaca tcacgtcca gaaaaagaa ggagtggtt catatagtc gaaaattcg ttaaattaa ttatgggcc taatagtac aacaagatt gttttaaat aaggcagtt ttcacggtt gtgaaaatt tatttacat tgaaagacc gtttatttt tttattgtt tttatttag gatgtgttc atgtggcat aaacttttg ttttgtgca atttctgta cttttttga cttagtttc tttccgtcc attttattt taggagtcg cagactccg cgtatttaa caacattcc agtattatt tatggttct aaaacacta aaattcgat cacttgatc ttccaaaag gttagtcgc tagaataag tgccttttt acggcatgt catcgatac tttgcactg taacttatc ttttgttgc ttttttaaa ttgtattat attgtaata ctgactagt atgagatat tgtgtaatt ttttacaaa taaaaatac ataaaacac
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