Status
Please wait ...
Gene information
General information
TC011250 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 660457..662335
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011250
    iBeetle screen iB_05002

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature lateral number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    leg musculature distal pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature lateral sometimesorientation irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    legs tibiotarsus shortened - Penetrance (percentage of animals/eggs on slide): more than 80%

    legs tibiotarsus fused - Penetrance (percentage of animals/eggs on slide): more than 80%

    legs femur shortened - Penetrance (percentage of animals/eggs on slide): more than 80%

    legs femur fused - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 7 (16 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011250 RA
      MSRQLNGSV TPEKRLGQN SIVQPLLTD LYQITMAYA 
      YWKSGKIQD HAVFDLFFR KNPFQGEFT IFAGLEECV 
      KFLDKFSYS KSDIDYIRQ ILPPTVEEE FYKYLGTLD 
      ARGVSFYAV KEGSVVFPR IPLLRVEGP LIVVQLLET 
      TLLNLVNYA SLIATNASR YRMASNKLK LYEFGLRRA 
      QGPDGALSA SKYSYIGGF DGTSNVLAG KLFNIPVKG 
      THAHAYVTS FNNLSELVI KTLEPKTGG KPVDFVALA 
      IKWRESIVS VFNVMLSEV NDGEFAAFI SFAISFPNG 
      YLALVDTYD VKKSGLVNF SAVALALLD LGYEPIGIR 
      LDSGDLAYL SVVARNLFI KISEQFKRP QFANLMIMA 
      SNDINEETI LSLNEQGHE INAFGIGTH LVTCQKQPA 
      LGGVYKMVE INGHARIKL SHDVGKVTI PGRKDVYRL 
      YGENGNALI DLLQKSSEA PPKVGEKVL CRHPFEESK 
      RAFVTPSKV EKLLVLYWQ NGKICQPLP NMQEIKERV 
      VDSLRILRA DIRRTLNPT PYKVAVSDD LYRYLHDLW 
      LENAPIGEL A
    CDS sequence
    • >TC011250 RA
      atgtcgcgc caactgaac ggttccgtc acccccgag 
      aagaggtta ggtcaaaac tcgatcgtg cagcccctt 
      ttgaccgac ttgtaccaa atcacgatg gcttacgcc 
      tactggaag tcgggaaaa attcaagac cacgccgtt 
      ttcgacctc ttcttccgc aaaaacccc ttccaaggt 
      gaattcacc atttttgca ggcctggaa gaatgcgtc 
      aaattcctc gataaattc tcgtattcg aagagcgac 
      atcgactac attcgacaa attcttccc cccactgtt 
      gaggaagag ttctacaag tatttgggc actttggac 
      gctcgagga gtgtctttt tacgccgtg aaagaaggc 
      agtgttgtc ttcccccgg attccgctg ctccgggtc 
      gagggcccc cttattgtt gtacaactt ctcgaaaca 
      actctactg aatcttgtg aattatgcc agtttgatc 
      gcaacaaac gcgtcgcgc tacagaatg gcttcaaac 
      aagctgaag ttgtatgag ttcgggttg aggcgggcc 
      caagggccc gatggggcc ctttctgcc tccaagtac 
      tcctacatt gggggcttt gacggtact agtaatgta 
      ctggcaggg aaattgttt aatatcccg gtgaaaggc 
      actcatgcc catgcttac gtcacttcc ttcaataat 
      ctaagtgag ctggtgatc aagacatta gaaccgaaa 
      acaggtggc aagcctgtc gatttcgtt gcgttggca 
      ataaaatgg cgcgagagt atagtttcg gttttcaac 
      gtcatgttg tcggaagtt aacgatggc gaattcgcc 
      gcttttatc tctttcgcc atttcgttc cctaatggt 
      tatttagca ctagttgac acttacgat gtaaaaaag 
      agcggtttg gtgaatttt agtgcagtc gcgttggct 
      ttgcttgat ttgggttat gaaccaatc gggattcga 
      ctagatagt ggcgacttg gcctactta tctgtcgtt 
      gctagaaat ctttttatt aaaataagc gagcaattc 
      aaacgccca caattcgcc aatttaatg ataatggcg 
      tccaatgac ataaacgag gagactatt ctaagttta 
      aacgagcaa ggtcacgag atcaacgcc tttggtatc 
      ggcacccat ttggtgact tgtcagaag caaccagcg 
      ctgggtggt gtgtacaag atggtggaa atcaacggc 
      catgccagg attaagctc agtcatgat gtgggtaaa 
      gttaccatt ccgggccgg aaggacgtc tatagactg 
      tatggagag aacgggaat gcgctcatt gatcttctg 
      cagaaatcg tcagaggcg ccgcccaaa gttggcgaa 
      aaggtgttg tgtcggcac ccgtttgag gagtcgaaa 
      cgggcgttt gttacgccg tcgaaagtc gagaagttg 
      ctggtgttg tattggcag aacgggaag atttgtcag 
      ccgttgccc aatatgcag gaaattaag gaacgggtt 
      gtggatagt ttgaggatt ttgagggcg gatattcgg 
      cgaacgcta aatccgacg ccgtataaa gtcgctgtt 
      agtgatgat ttgtatcgg tatttgcat gatttgtgg 
      ttggagaat gcacccatc ggggagttg gcttaa
    mRNA sequence
    • >TC011250 RA
      gtaccacct taactcccc caaagtcac ctccaaaaa 
      tgtaatcac cttcgaaaa tttaaaaac tgttcaacc 
      caacgagat gtgaatacg tttaagact tcaagtgca 
      cccaacccc acgatgtcg cgccaactg aacggttcc 
      gtcaccccc gagaagagg ttaggtcaa aactcgatc 
      gtgcagccc cttttgacc gacttgtac caaatcacg 
      atggcttac gcctactgg aagtcggga aaaattcaa 
      gaccacgcc gttttcgac ctcttcttc cgcaaaaac 
      cccttccaa ggtgaattc accattttt gcaggcctg 
      gaagaatgc gtcaaattc ctcgataaa ttctcgtat 
      tcgaagagc gacatcgac tacattcga caaattctt 
      ccccccact gttgaggaa gagttctac aagtatttg 
      ggcactttg gacgctcga ggagtgtct ttttacgcc 
      gtgaaagaa ggcagtgtt gtcttcccc cggattccg 
      ctgctccgg gtcgagggc ccccttatt gttgtacaa 
      cttctcgaa acaactcta ctgaatctt gtgaattat 
      gccagtttg atcgcaaca aacgcgtcg cgctacaga 
      atggcttca aacaagctg aagttgtat gagttcggg 
      ttgaggcgg gcccaaggg cccgatggg gccctttct 
      gcctccaag tactcctac attgggggc tttgacggt 
      actagtaat gtactggca gggaaattg tttaatatc 
      ccggtgaaa ggcactcat gcccatgct tacgtcact 
      tccttcaat aatctaagt gagctggtg atcaagaca 
      ttagaaccg aaaacaggt ggcaagcct gtcgatttc 
      gttgcgttg gcaataaaa tggcgcgag agtatagtt 
      tcggttttc aacgtcatg ttgtcggaa gttaacgat 
      ggcgaattc gccgctttt atctctttc gccatttcg 
      ttccctaat ggttattta gcactagtt gacacttac 
      gatgtaaaa aagagcggt ttggtgaat tttagtgca 
      gtcgcgttg gctttgctt gatttgggt tatgaacca 
      atcgggatt cgactagat agtggcgac ttggcctac 
      ttatctgtc gttgctaga aatcttttt attaaaata 
      agcgagcaa ttcaaacgc ccacaattc gccaattta 
      atgataatg gcgtccaat gacataaac gaggagact 
      attctaagt ttaaacgag caaggtcac gagatcaac 
      gcctttggt atcggcacc catttggtg acttgtcag 
      aagcaacca gcgctgggt ggtgtgtac aagatggtg 
      gaaatcaac ggccatgcc aggattaag ctcagtcat 
      gatgtgggt aaagttacc attccgggc cggaaggac 
      gtctataga ctgtatgga gagaacggg aatgcgctc 
      attgatctt ctgcagaaa tcgtcagag gcgccgccc 
      aaagttggc gaaaaggtg ttgtgtcgg cacccgttt 
      gaggagtcg aaacgggcg tttgttacg ccgtcgaaa 
      gtcgagaag ttgctggtg ttgtattgg cagaacggg 
      aagatttgt cagccgttg cccaatatg caggaaatt 
      aaggaacgg gttgtggat agtttgagg attttgagg 
      gcggatatt cggcgaacg ctaaatccg acgccgtat 
      aaagtcgct gttagtgat gatttgtat cggtatttg 
      catgatttg tggttggag aatgcaccc atcggggag 
      ttggcttaa ggagggcct tggccaagt tttagtatt 
      ttatataac attctgtat tttgcttat tttttcatg 
      ttatactgt ttataatag attataata taaaggttt 
      atcttgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY