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Gene information
General information
TC011233 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 651017..657805
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011233
    iBeetle screen iB_04998

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011233 RA
      MWDSTMFLS TLTPKFYVA LTGTSSLIS GLILIFEWW 
      YFRKYGTSF IEQISLNHI SPWISGVDS SENGSSSNS 
      TSSQSSVPE CKVWRNPLN LYRGAEYQR FYWATNKEP 
      LTYYDMNLS AQDHQTFFT CEADAGKSE YEIMQTAWR 
      ERNPMVRIK AAHSALEKS PECAPAYIL LAEEEATTI 
      TEAEKILKQ ALKVAETNY RKSQALQHQ GSLMEAQHR 
      RDTNVLIYI KRRLAMCAR RLGKLKEAV KMFRDLTKE 
      VPPIMNVLN IHENLIEAL LEMQAYADV QAVLAKYDD 
      ISLPKSATI CYTAALLKA RLVADKFSP DIASKRGLN 
      TAEMAAVEA IHRAVEFNP HVPKYLLEM KPLILPPEH 
      VLKRGDSEA IAYTFFHLA HWKAVDGAL NLLHCTWEG 
      TFRMLPYPL ERGHLFYPY PTCTECADR ELLPTFHEV 
      SVYPKKELP FFIIFTAGL CSFTALLTL LTHQYPEPM 
      GVVAKAVLN WLSTPVYYV YEKIESMLP ANLLQQLSR 
      I
    CDS sequence
    • >TC011233 RA
      atgtgggac tctacaatg ttcctgagc accctcacg 
      ccaaaattt tacgtcgct ttaaccggc acttcgtcc 
      ctaatctcc ggcttgatc ctcattttc gaatggtgg 
      tatttccgc aagtacggc acgagtttc atcgaacaa 
      atttccctc aaccacatc agcccctgg atcagtggc 
      gtcgacagc agcgaaaac ggaagttcg agtaattcg 
      actagttcg caaagtagc gttcctgag tgtaaggtg 
      tggagaaat ccgctgaat ttgtacagg ggagccgaa 
      taccaaaga ttctactgg gctactaat aaggagcct 
      ttgacttat tatgatatg aatttgtcg gctcaggac 
      caccagacg ttttttacg tgtgaggcg gatgcgggt 
      aaatctgag tatgagatt atgcaaact gcctggcgg 
      gagaggaac cccatggtg aggattaaa gctgcacac 
      agtgctttg gagaagagc cctgagtgt gcccccgct 
      tatattttg ttggcggaa gaagaggca acgacgata 
      actgaagcc gagaaaatt ctaaaacag gccctcaaa 
      gtggcagaa acgaactat agaaagtca caagccctg 
      caacaccaa gggagcctg atggaagcg cagcaccgg 
      cgggacacc aacgtctta atttatata aaaagaagg 
      ttggcaatg tgtgcccgc cgcctcggg aagctcaaa 
      gaagctgta aaaatgttc cgcgattta accaaagaa 
      gtcccccca ataatgaac gttttaaac attcacgaa 
      aacctcatc gaagcattg ctcgaaatg caggcatac 
      gccgacgtt caggccgtg ttggcaaaa tacgacgac 
      atctccttg ccaaaatcc gccaccatc tgctacacg 
      gccgccctc ctcaaagcc cgtctagtc gccgacaag 
      ttctccccg gacatcgcc agcaagcgg ggcctgaac 
      acggccgaa atggccgcc gtggaggcc atccaccgc 
      gccgtggag ttcaacccc cacgtgccc aaatacctg 
      ctggagatg aagccgctc atcctgccc ccggagcac 
      gtgctgaag cgcggcgac tcggaggcc atcgcctac 
      acgttcttc cacctggcg cactggaag gcggtggac 
      ggcgccctc aacctgctg cactgcacg tgggagggc 
      accttccgc atgctgccc taccccctg gagcgcgga 
      cacctcttc tacccctac cccacgtgc acggagtgc 
      gccgatcgg gagttgttg cccaccttc cacgaagtc 
      tccgtttat ccgaagaag gagctgccg ttttttatt 
      atttttacg gcggggttg tgttcgttt acggcgctt 
      ctgacgctg ttaacgcat cagtatcct gagccgatg 
      ggggttgtg gccaaagcg gtcctgaat tggctctct 
      acgccggtg tattatgtc tatgagaaa atcgaaagt 
      atgctgccg gcgaatttg ctgcagcag ttgtcgaga 
      atttaa
    mRNA sequence
    • >TC011233 RA
      tactccacc ccaacaaat gtcgaaaca attcgaaac 
      aggtcttca aaattttga gtccagccc tgcccacct 
      cgtgttttg aaaatgtca accgtttaa cgtcaccaa 
      atgtcataa acactttaa gtgaaccaa aattctggt 
      acttccacc aaaaatagc accaaagcg ttaatgacc 
      gcgtgaacc gcaccgcct taacgcccc aaatcagag 
      taatgttct aaaaactcg tgtcgcaaa tcagttttt 
      gtccgacat aacctcaaa catgtggga ctctacaat 
      gttcctgag caccctcac gccaaaatt ttacgtcgc 
      tttaaccgg cacttcgtc cctaatctc cggcttgat 
      cctcatttt cgaatggtg gtatttccg caagtacgg 
      cacgagttt catcgaaca aatttccct caaccacat 
      cagcccctg gatcagtgg cgtcgacag cagcgaaaa 
      cggaagttc gagtaattc gactagttc gcaaagtag 
      cgttcctga gtgtaaggt gtggagaaa tccgctgaa 
      tttgtacag gggagccga ataccaaag attctactg 
      ggctactaa taaggagcc tttgactta ttatgatat 
      gaatttgtc ggctcagga ccaccagac gttttttac 
      gtgtgaggc ggatgcggg taaatctga gtatgagat 
      tatgcaaac tgcctggcg ggagaggaa ccccatggt 
      gaggattaa agctgcaca cagtgcttt ggagaagag 
      ccctgagtg tgcccccgc ttatatttt gttggcgga 
      agaagaggc aacgacgat aactgaagc cgagaaaat 
      tctaaaaca ggccctcaa agtggcaga aacgaacta 
      tagaaagtc acaagccct gcaacacca agggagcct 
      gatggaagc gcagcaccg gcgggacac caacgtctt 
      aatttatat aaaaagaag gttggcaat gtgtgcccg 
      ccgcctcgg gaagctcaa agaagctgt aaaaatgtt 
      ccgcgattt aaccaaaga agtcccccc aataatgaa 
      cgttttaaa cattcacga aaacctcat cgaagcatt 
      gctcgaaat gcaggcata cgccgacgt tcaggccgt 
      gttggcaaa atacgacga catctcctt gccaaaatc 
      cgccaccat ctgctacac ggccgccct cctcaaagc 
      ccgtctagt cgccgacaa gttctcccc ggacatcgc 
      cagcaagcg gggcctgaa cacggccga aatggccgc 
      cgtggaggc catccaccg cgccgtgga gttcaaccc 
      ccacgtgcc caaatacct gctggagat gaagccgct 
      catcctgcc cccggagca cgtgctgaa gcgcggcga 
      ctcggaggc catcgccta cacgttctt ccacctggc 
      gcactggaa ggcggtgga cggcgccct caacctgct 
      gcactgcac gtgggaggg caccttccg catgctgcc 
      ctaccccct ggagcgcgg acacctctt ctaccccta 
      ccccacgtg cacggagtg cgccgatcg ggagttgtt 
      gcccacctt ccacgaagt ctccgttta tccgaagaa 
      ggagctgcc gttttttat tatttttac ggcggggtt 
      gtgttcgtt tacggcgct tctgacgct gttaacgca 
      tcagtatcc tgagccgat gggggttgt ggccaaagc 
      ggtcctgaa ttggctctc tacgccggt gtattatgt 
      ctatgagaa aatcgaaag tatgctgcc ggcgaattt 
      gctgcagca gttgtcgag aatttaatt cgagacgaa 
      gttcgaacg tactgtact gtgtggtag gttaggtag 
      ctcatgtat gggttttta tatagatag gtgtttttt 
      aacttattt tcaaagtga ttatatttt gtgttatga 
      ttgtgagct tttttatgt ctgatttta tttttgttt 
      tttttcttg tcactatgt attcaaaga tgtttgccc 
      aaacttatt atttaagga aaatgtgat tatgcaaat 
      aaatgacac tgaatggc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY