Status
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Gene information
General information
TC011233 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 651017..657805
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC011233
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04998

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011233 RA
      MWDSTMFLS TLTPKFYVA LTGTSSLIS GLILIFEWW 
YFRKYGTSF IEQISLNHI SPWISGVDS SENGSSSNS 
TSSQSSVPE CKVWRNPLN LYRGAEYQR FYWATNKEP 
LTYYDMNLS AQDHQTFFT CEADAGKSE YEIMQTAWR 
ERNPMVRIK AAHSALEKS PECAPAYIL LAEEEATTI 
TEAEKILKQ ALKVAETNY RKSQALQHQ GSLMEAQHR 
RDTNVLIYI KRRLAMCAR RLGKLKEAV KMFRDLTKE 
VPPIMNVLN IHENLIEAL LEMQAYADV QAVLAKYDD 
ISLPKSATI CYTAALLKA RLVADKFSP DIASKRGLN 
TAEMAAVEA IHRAVEFNP HVPKYLLEM KPLILPPEH 
VLKRGDSEA IAYTFFHLA HWKAVDGAL NLLHCTWEG 
TFRMLPYPL ERGHLFYPY PTCTECADR ELLPTFHEV 
SVYPKKELP FFIIFTAGL CSFTALLTL LTHQYPEPM 
GVVAKAVLN WLSTPVYYV YEKIESMLP ANLLQQLSR 
I
    CDS sequence
    • >TC011233 RA
      atgtgggac tctacaatg ttcctgagc accctcacg 
ccaaaattt tacgtcgct ttaaccggc acttcgtcc 
ctaatctcc ggcttgatc ctcattttc gaatggtgg 
tatttccgc aagtacggc acgagtttc atcgaacaa 
atttccctc aaccacatc agcccctgg atcagtggc 
gtcgacagc agcgaaaac ggaagttcg agtaattcg 
actagttcg caaagtagc gttcctgag tgtaaggtg 
tggagaaat ccgctgaat ttgtacagg ggagccgaa 
taccaaaga ttctactgg gctactaat aaggagcct 
ttgacttat tatgatatg aatttgtcg gctcaggac 
caccagacg ttttttacg tgtgaggcg gatgcgggt 
aaatctgag tatgagatt atgcaaact gcctggcgg 
gagaggaac cccatggtg aggattaaa gctgcacac 
agtgctttg gagaagagc cctgagtgt gcccccgct 
tatattttg ttggcggaa gaagaggca acgacgata 
actgaagcc gagaaaatt ctaaaacag gccctcaaa 
gtggcagaa acgaactat agaaagtca caagccctg 
caacaccaa gggagcctg atggaagcg cagcaccgg 
cgggacacc aacgtctta atttatata aaaagaagg 
ttggcaatg tgtgcccgc cgcctcggg aagctcaaa 
gaagctgta aaaatgttc cgcgattta accaaagaa 
gtcccccca ataatgaac gttttaaac attcacgaa 
aacctcatc gaagcattg ctcgaaatg caggcatac 
gccgacgtt caggccgtg ttggcaaaa tacgacgac 
atctccttg ccaaaatcc gccaccatc tgctacacg 
gccgccctc ctcaaagcc cgtctagtc gccgacaag 
ttctccccg gacatcgcc agcaagcgg ggcctgaac 
acggccgaa atggccgcc gtggaggcc atccaccgc 
gccgtggag ttcaacccc cacgtgccc aaatacctg 
ctggagatg aagccgctc atcctgccc ccggagcac 
gtgctgaag cgcggcgac tcggaggcc atcgcctac 
acgttcttc cacctggcg cactggaag gcggtggac 
ggcgccctc aacctgctg cactgcacg tgggagggc 
accttccgc atgctgccc taccccctg gagcgcgga 
cacctcttc tacccctac cccacgtgc acggagtgc 
gccgatcgg gagttgttg cccaccttc cacgaagtc 
tccgtttat ccgaagaag gagctgccg ttttttatt 
atttttacg gcggggttg tgttcgttt acggcgctt 
ctgacgctg ttaacgcat cagtatcct gagccgatg 
ggggttgtg gccaaagcg gtcctgaat tggctctct 
acgccggtg tattatgtc tatgagaaa atcgaaagt 
atgctgccg gcgaatttg ctgcagcag ttgtcgaga 
atttaa
    mRNA sequence
    • >TC011233 RA
      tactccacc ccaacaaat gtcgaaaca attcgaaac 
aggtcttca aaattttga gtccagccc tgcccacct 
cgtgttttg aaaatgtca accgtttaa cgtcaccaa 
atgtcataa acactttaa gtgaaccaa aattctggt 
acttccacc aaaaatagc accaaagcg ttaatgacc 
gcgtgaacc gcaccgcct taacgcccc aaatcagag 
taatgttct aaaaactcg tgtcgcaaa tcagttttt 
gtccgacat aacctcaaa catgtggga ctctacaat 
gttcctgag caccctcac gccaaaatt ttacgtcgc 
tttaaccgg cacttcgtc cctaatctc cggcttgat 
cctcatttt cgaatggtg gtatttccg caagtacgg 
cacgagttt catcgaaca aatttccct caaccacat 
cagcccctg gatcagtgg cgtcgacag cagcgaaaa 
cggaagttc gagtaattc gactagttc gcaaagtag 
cgttcctga gtgtaaggt gtggagaaa tccgctgaa 
tttgtacag gggagccga ataccaaag attctactg 
ggctactaa taaggagcc tttgactta ttatgatat 
gaatttgtc ggctcagga ccaccagac gttttttac 
gtgtgaggc ggatgcggg taaatctga gtatgagat 
tatgcaaac tgcctggcg ggagaggaa ccccatggt 
gaggattaa agctgcaca cagtgcttt ggagaagag 
ccctgagtg tgcccccgc ttatatttt gttggcgga 
agaagaggc aacgacgat aactgaagc cgagaaaat 
tctaaaaca ggccctcaa agtggcaga aacgaacta 
tagaaagtc acaagccct gcaacacca agggagcct 
gatggaagc gcagcaccg gcgggacac caacgtctt 
aatttatat aaaaagaag gttggcaat gtgtgcccg 
ccgcctcgg gaagctcaa agaagctgt aaaaatgtt 
ccgcgattt aaccaaaga agtcccccc aataatgaa 
cgttttaaa cattcacga aaacctcat cgaagcatt 
gctcgaaat gcaggcata cgccgacgt tcaggccgt 
gttggcaaa atacgacga catctcctt gccaaaatc 
cgccaccat ctgctacac ggccgccct cctcaaagc 
ccgtctagt cgccgacaa gttctcccc ggacatcgc 
cagcaagcg gggcctgaa cacggccga aatggccgc 
cgtggaggc catccaccg cgccgtgga gttcaaccc 
ccacgtgcc caaatacct gctggagat gaagccgct 
catcctgcc cccggagca cgtgctgaa gcgcggcga 
ctcggaggc catcgccta cacgttctt ccacctggc 
gcactggaa ggcggtgga cggcgccct caacctgct 
gcactgcac gtgggaggg caccttccg catgctgcc 
ctaccccct ggagcgcgg acacctctt ctaccccta 
ccccacgtg cacggagtg cgccgatcg ggagttgtt 
gcccacctt ccacgaagt ctccgttta tccgaagaa 
ggagctgcc gttttttat tatttttac ggcggggtt 
gtgttcgtt tacggcgct tctgacgct gttaacgca 
tcagtatcc tgagccgat gggggttgt ggccaaagc 
ggtcctgaa ttggctctc tacgccggt gtattatgt 
ctatgagaa aatcgaaag tatgctgcc ggcgaattt 
gctgcagca gttgtcgag aatttaatt cgagacgaa 
gttcgaacg tactgtact gtgtggtag gttaggtag 
ctcatgtat gggttttta tatagatag gtgtttttt 
aacttattt tcaaagtga ttatatttt gtgttatga 
ttgtgagct tttttatgt ctgatttta tttttgttt 
tttttcttg tcactatgt attcaaaga tgtttgccc 
aaacttatt atttaagga aaatgtgat tatgcaaat 
aaatgacac tgaatggc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY