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Gene information
General information
TC011231 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 759444..770742
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011231
    iBeetle screen iB_04997

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    vitellarium not present - Number of animals showing the phenotype: 3

    tropharium size decreased - Number of animals showing the phenotype: 3

    ovary without detectable defects - Number of animals showing the phenotype: 1
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    maxillary palp present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    previtellogenic egg chamber mostlynot present - Number of animals showing the phenotype: 4

    vitellogenic egg chamber not present - Number of animals showing the phenotype: 4
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011231 RA
      MFEKIFQSL DVTSLLIIA IFFLFLEYR PPWWYRNND 
      CKKGVKLIP GPLALPGLG TTWIFFFGG FSFNRLHLY 
      YENMYKRYG PVMKEEYWC NIPVINLFE KREIVKVLK 
      AGGKYPLRP PVEAVAHYR RSRPDRYAS IGLVNEQGE 
      AWYDLRSTL TPALTSPKT ITSFLPEAQ QIADDWCNL 
      LKLSRDKNG RVSNLNYIA DRLGLELTC ALVLGRRMG 
      FLLPGAETE TGEKLAEAV RQHFLGTRD TYFGFPFWK 
      LFPTPAYKT LIKSEASIY ELALELINS ANESTKESA 
      VFQSVIQAE IDEREKIAA IIDFISAGI HTMKNSLLF 
      LLHLIGQDL QIQKKIIED STKSYSKAC VTETFRLLP 
      TANALGRIL EEEMELGGY RLSAGTVVV CHFGIACRD 
      ERNFPDASK FKPERWLDD DKVQTATNS LFLLTPFGA 
      GRRICPGKR FIEHILPLL LESTVNSFE IQAEEELEL 
      QFEFLVTPK GQMPMVFKD RV
    CDS sequence
    • >TC011231 RA
      atgtttgag aagattttt cagtcgttg gacgtcaca 
      agtttgctc atcatcgcg atcttcttc ctgttcttg 
      gagtacagg cctccgtgg tggtaccgc aataatgat 
      tgcaagaaa ggtgttaaa ctcataccg ggacccttg 
      gctttacct gggcttggt actacctgg atatttttt 
      ttcggaggg ttttccttc aaccgactg cacctgtat 
      tacgaaaac atgtacaaa aggtatgga cctgtcatg 
      aaagaagaa tattggtgt aatatacct gttattaac 
      ttatttgag aaaagagaa attgtgaag gttcttaaa 
      gcgggtggc aagtatcct ctaagacca cctgttgaa 
      gcagtcgcc cactatcga agaagtcgc cctgataga 
      tacgctagc attgggttg gtcaacgaa caaggtgag 
      gcatggtac gacctccgc agcaccctc acccccgct 
      ctcaccagc cccaaaaca atcaccagc ttcctcccc 
      gaagctcag caaatcgcc gacgactgg tgcaacctc 
      ctcaaacta agccgtgac aaaaacggc agagtatcc 
      aacctaaac tacatcgca gaccgactc ggcctggag 
      ctaacctgc gccctagtc ctcggacgc agaatgggc 
      ttcctcctg ccaggcgcc gaaaccgaa accggggaa 
      aaactcgcc gaagccgtc cgacaacac ttcctcggc 
      acccgagac acctacttc ggcttcccc ttctggaaa 
      ctcttccca acaccggcc tacaaaaca ctcatcaaa 
      agcgaggct tccatctac gaactcgcc ctagaactg 
      atcaattct gccaacgaa tcgaccaaa gaaagtgcc 
      gttttccag tcagttatc caagcggaa attgacgaa 
      agggagaaa atcgccgcc attattgac ttcatatct 
      gcggggatt cacaccatg aagaacagt cttttgttt 
      ttgctgcac ctgattgga caagacctc caaatacag 
      aagaaaatc atcgaggat tcgaccaaa tcgtactcg 
      aaagcgtgc gttactgag acttttcgt ctgttacca 
      actgccaac gctttaggg aggatcctg gaggaggag 
      atggaactt ggaggttac cgtttaagt gcaggaaca 
      gtggtcgtg tgtcacttt gggattgcc tgccgagac 
      gaacgcaat tttccggac gcttcaaag ttcaaaccg 
      gagcgatgg ctggatgat gataaggtg caaactgcg 
      actaattcc ctgtttctt ttgacccct ttcggggcc 
      ggccggagg atttgtccc ggaaagaga tttatcgaa 
      catatttta ccgctgttg cttgagtcc actgtcaat 
      tcgtttgag attcaagcg gaagaggag ctcgagttg 
      cagtttgag tttttggtg acgccgaaa ggacaaatg 
      ccgatggtt tttaaagat agagtctag
    mRNA sequence
    • >TC011231 RA
      acacattgt tgaaaccga atctgtgcg atgttgacg 
      tgagaaata attaatctt caatattct gacctcgtc 
      agaaccttg acccgctaa agcactctc ttcttacca 
      tccatttat ctgataaga taagtcgaa aaaagccgg 
      caaaatccg cgcgctatt caatatctt acacaaatg 
      tcagaaaga tgatgaaga gaacttccg ttaagagtc 
      tggaatgtt ggagatgcg gctgttgaa ccattcttc 
      aacagttta tacaaaaga cccaagcaa gacgctacg 
      agaccgttc ttttaacct aaaatcgcc ggtaatctc 
      agttaatgt cttaaaata cacaaacga taatcgtgt 
      gggttatcg agtgctcgt ctcgacggt tttgaaacc 
      ccaagatgt ttgagaaga tttttcagt cgttggacg 
      tcacaagtt tgctcatca tcgcgatct tcttcctgt 
      tcttggagt acaggcctc cgtggtggt accgcaata 
      atgattgca agaaaggtg ttaaactca taccgggac 
      ccttggctt tacctgggc ttggtacta cctggatat 
      tttttttcg gagggtttt ccttcaacc gactgcacc 
      tgtattacg aaaacatgt acaaaaggt atggacctg 
      tcatgaaag aagaatatt ggtgtaata tacctgtta 
      ttaacttat ttgagaaaa gagaaattg tgaaggttc 
      ttaaagcgg gtggcaagt atcctctaa gaccacctg 
      ttgaagcag tcgcccact atcgaagaa gtcgccctg 
      atagatacg ctagcattg ggttggtca acgaacaag 
      gtgaggcat ggtacgacc tccgcagca ccctcaccc 
      ccgctctca ccagcccca aaacaatca ccagcttcc 
      tccccgaag ctcagcaaa tcgccgacg actggtgca 
      acctcctca aactaagcc gtgacaaaa acggcagag 
      tatccaacc taaactaca tcgcagacc gactcggcc 
      tggagctaa cctgcgccc tagtcctcg gacgcagaa 
      tgggcttcc tcctgccag gcgccgaaa ccgaaaccg 
      gggaaaaac tcgccgaag ccgtccgac aacacttcc 
      tcggcaccc gagacacct acttcggct tccccttct 
      ggaaactct tcccaacac cggcctaca aaacactca 
      tcaaaagcg aggcttcca tctacgaac tcgccctag 
      aactgatca attctgcca acgaatcga ccaaagaaa 
      gtgccgttt tccagtcag ttatccaag cggaaattg 
      acgaaaggg agaaaatcg ccgccatta ttgacttca 
      tatctgcgg ggattcaca ccatgaaga acagtcttt 
      tgtttttgc tgcacctga ttggacaag acctccaaa 
      tacagaaga aaatcatcg aggattcga ccaaatcgt 
      actcgaaag cgtgcgtta ctgagactt ttcgtctgt 
      taccaactg ccaacgctt tagggagga tcctggagg 
      aggagatgg aacttggag gttaccgtt taagtgcag 
      gaacagtgg tcgtgtgtc actttggga ttgcctgcc 
      gagacgaac gcaattttc cggacgctt caaagttca 
      aaccggagc gatggctgg atgatgata aggtgcaaa 
      ctgcgacta attccctgt ttcttttga cccctttcg 
      gggccggcc ggaggattt gtcccggaa agagattta 
      tcgaacata ttttaccgc tgttgcttg agtccactg 
      tcaattcgt ttgagattc aagcggaag aggagctcg 
      agttgcagt ttgagtttt tggtgacgc cgaaaggac 
      aaatgccga tggttttta aagatagag tctagatgt 
      tttaattag tgtagctga tcaatatgt catgtttta 
      agtggctgt gtactggca tcatgtgtt cattttctt 
      tcattccac tattttaat tttttaata aagttgttt 
      tagcaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY