General information | |||
TC011231 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007425.3: 759444..770742 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC011231 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_04997 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)tropharium size decreased - Number of animals showing the phenotype: 3
ovary without detectable defects - Number of animals showing the phenotype: 1
cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
maxillary palp present - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
previtellogenic egg chamber mostlynot present - Number of animals showing the phenotype: 4
vitellogenic egg chamber not present - Number of animals showing the phenotype: 4
fertility after larval injection decreased (no living offspring) -
- >TC011231 RA
MFEKIFQSL DVTSLLIIA IFFLFLEYR PPWWYRNND CKKGVKLIP GPLALPGLG TTWIFFFGG FSFNRLHLY YENMYKRYG PVMKEEYWC NIPVINLFE KREIVKVLK AGGKYPLRP PVEAVAHYR RSRPDRYAS IGLVNEQGE AWYDLRSTL TPALTSPKT ITSFLPEAQ QIADDWCNL LKLSRDKNG RVSNLNYIA DRLGLELTC ALVLGRRMG FLLPGAETE TGEKLAEAV RQHFLGTRD TYFGFPFWK LFPTPAYKT LIKSEASIY ELALELINS ANESTKESA VFQSVIQAE IDEREKIAA IIDFISAGI HTMKNSLLF LLHLIGQDL QIQKKIIED STKSYSKAC VTETFRLLP TANALGRIL EEEMELGGY RLSAGTVVV CHFGIACRD ERNFPDASK FKPERWLDD DKVQTATNS LFLLTPFGA GRRICPGKR FIEHILPLL LESTVNSFE IQAEEELEL QFEFLVTPK GQMPMVFKD RV
- >TC011231 RA
atgtttgag aagattttt cagtcgttg gacgtcaca agtttgctc atcatcgcg atcttcttc ctgttcttg gagtacagg cctccgtgg tggtaccgc aataatgat tgcaagaaa ggtgttaaa ctcataccg ggacccttg gctttacct gggcttggt actacctgg atatttttt ttcggaggg ttttccttc aaccgactg cacctgtat tacgaaaac atgtacaaa aggtatgga cctgtcatg aaagaagaa tattggtgt aatatacct gttattaac ttatttgag aaaagagaa attgtgaag gttcttaaa gcgggtggc aagtatcct ctaagacca cctgttgaa gcagtcgcc cactatcga agaagtcgc cctgataga tacgctagc attgggttg gtcaacgaa caaggtgag gcatggtac gacctccgc agcaccctc acccccgct ctcaccagc cccaaaaca atcaccagc ttcctcccc gaagctcag caaatcgcc gacgactgg tgcaacctc ctcaaacta agccgtgac aaaaacggc agagtatcc aacctaaac tacatcgca gaccgactc ggcctggag ctaacctgc gccctagtc ctcggacgc agaatgggc ttcctcctg ccaggcgcc gaaaccgaa accggggaa aaactcgcc gaagccgtc cgacaacac ttcctcggc acccgagac acctacttc ggcttcccc ttctggaaa ctcttccca acaccggcc tacaaaaca ctcatcaaa agcgaggct tccatctac gaactcgcc ctagaactg atcaattct gccaacgaa tcgaccaaa gaaagtgcc gttttccag tcagttatc caagcggaa attgacgaa agggagaaa atcgccgcc attattgac ttcatatct gcggggatt cacaccatg aagaacagt cttttgttt ttgctgcac ctgattgga caagacctc caaatacag aagaaaatc atcgaggat tcgaccaaa tcgtactcg aaagcgtgc gttactgag acttttcgt ctgttacca actgccaac gctttaggg aggatcctg gaggaggag atggaactt ggaggttac cgtttaagt gcaggaaca gtggtcgtg tgtcacttt gggattgcc tgccgagac gaacgcaat tttccggac gcttcaaag ttcaaaccg gagcgatgg ctggatgat gataaggtg caaactgcg actaattcc ctgtttctt ttgacccct ttcggggcc ggccggagg atttgtccc ggaaagaga tttatcgaa catatttta ccgctgttg cttgagtcc actgtcaat tcgtttgag attcaagcg gaagaggag ctcgagttg cagtttgag tttttggtg acgccgaaa ggacaaatg ccgatggtt tttaaagat agagtctag
- >TC011231 RA
acacattgt tgaaaccga atctgtgcg atgttgacg tgagaaata attaatctt caatattct gacctcgtc agaaccttg acccgctaa agcactctc ttcttacca tccatttat ctgataaga taagtcgaa aaaagccgg caaaatccg cgcgctatt caatatctt acacaaatg tcagaaaga tgatgaaga gaacttccg ttaagagtc tggaatgtt ggagatgcg gctgttgaa ccattcttc aacagttta tacaaaaga cccaagcaa gacgctacg agaccgttc ttttaacct aaaatcgcc ggtaatctc agttaatgt cttaaaata cacaaacga taatcgtgt gggttatcg agtgctcgt ctcgacggt tttgaaacc ccaagatgt ttgagaaga tttttcagt cgttggacg tcacaagtt tgctcatca tcgcgatct tcttcctgt tcttggagt acaggcctc cgtggtggt accgcaata atgattgca agaaaggtg ttaaactca taccgggac ccttggctt tacctgggc ttggtacta cctggatat tttttttcg gagggtttt ccttcaacc gactgcacc tgtattacg aaaacatgt acaaaaggt atggacctg tcatgaaag aagaatatt ggtgtaata tacctgtta ttaacttat ttgagaaaa gagaaattg tgaaggttc ttaaagcgg gtggcaagt atcctctaa gaccacctg ttgaagcag tcgcccact atcgaagaa gtcgccctg atagatacg ctagcattg ggttggtca acgaacaag gtgaggcat ggtacgacc tccgcagca ccctcaccc ccgctctca ccagcccca aaacaatca ccagcttcc tccccgaag ctcagcaaa tcgccgacg actggtgca acctcctca aactaagcc gtgacaaaa acggcagag tatccaacc taaactaca tcgcagacc gactcggcc tggagctaa cctgcgccc tagtcctcg gacgcagaa tgggcttcc tcctgccag gcgccgaaa ccgaaaccg gggaaaaac tcgccgaag ccgtccgac aacacttcc tcggcaccc gagacacct acttcggct tccccttct ggaaactct tcccaacac cggcctaca aaacactca tcaaaagcg aggcttcca tctacgaac tcgccctag aactgatca attctgcca acgaatcga ccaaagaaa gtgccgttt tccagtcag ttatccaag cggaaattg acgaaaggg agaaaatcg ccgccatta ttgacttca tatctgcgg ggattcaca ccatgaaga acagtcttt tgtttttgc tgcacctga ttggacaag acctccaaa tacagaaga aaatcatcg aggattcga ccaaatcgt actcgaaag cgtgcgtta ctgagactt ttcgtctgt taccaactg ccaacgctt tagggagga tcctggagg aggagatgg aacttggag gttaccgtt taagtgcag gaacagtgg tcgtgtgtc actttggga ttgcctgcc gagacgaac gcaattttc cggacgctt caaagttca aaccggagc gatggctgg atgatgata aggtgcaaa ctgcgacta attccctgt ttcttttga cccctttcg gggccggcc ggaggattt gtcccggaa agagattta tcgaacata ttttaccgc tgttgcttg agtccactg tcaattcgt ttgagattc aagcggaag aggagctcg agttgcagt ttgagtttt tggtgacgc cgaaaggac aaatgccga tggttttta aagatagag tctagatgt tttaattag tgtagctga tcaatatgt catgtttta agtggctgt gtactggca tcatgtgtt cattttctt tcattccac tattttaat tttttaata aagttgttt tagcaac
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
