Status
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Gene information
General information
TC011230 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 775061..798472
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011230
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04996

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011230 RA
      MIKVVLTWL TYLSAVSSI PQFDPTALL QGILIPADV 
      AVGTIIYRL RAHDPTFEY PLVFSLQNE PTTVSITSL 
      NCTRLNSIC QANVVLRRK LKVDLFYDF NVIVQNQRG 
      ETAGLNCSF RATNATTPI NEIFPGAPT LLSVSESAR 
      RNAELGSII AKGNPNRPD GVLLELWGA AQFGLYQKL 
      ITEKDAQAT IVLLGALDY ETTNVHHLT ILANDPWTR 
      MQEDTRNIA SWSLLIAVI DEQDTPPIF TLAPPTTTL 
      SPSLLPGDV VLRIHAEDG DRGNPRDIR YGLVPENNP 
      FTPFFNISE ETGELRLVR PLNEIVAIS HSGQPILLT 
      VIAEEVRTN PQEPSAQSS TVSLALIPP GIRPGRPIF 
      GSSEYQTLL DENSQSGTV LNLPQAEVV AQPGDVVAL 
      TLVGNNNTF EIAPSVVEG QSKFEIRVH DPKLLDYEK 
      WHSVQCYIV AREVGTGNF TAQAKLEVL LNDVNDNVP 
      EFSQEEYQG TIQENAPFG TTLLRVEAS DSDRAPGSK 
      IKYVRLYGD GSDFFSLDS TTGLVTVSD PSGLDAEKH 
      PKLSFFVEA ADEEGKGQT STASIIVRL IDINDEAPQ 
      FEKNVYEFI LNHDKTAFT TRAFIKAVD RDISPPNNE 
      IHYELIKPV EGLHLNERT GELVITRTW LESNIVITT 
      ARAWDGGVP RLFSECEIR IYPPESHSR RMVFIVPGK 
      NPDLADLQK KLSVLTGAK ITINEVRPY LGAEPGAKD 
      VSGYNSEKS VVVATATYS DDFIDVNTV KQVFDEKHT 
      TQIEKASQN GGNLLWLLI LLIILALLL AIILILCCI 
      CQGCPLYVP PKRRKVVSA EIKKVVRGS GNGKESKSV 
      QVAEWFGRR EAWSPEHVD NEAESLQRH EQERGSDRT 
      EARRTIHRP LQREPSREQ FYIREGNTD ILRLITRGN 
      DQRADQTYY ITDSGKDIL MRRFIDQQQ AETLKQVPL 
      PNSVTKLQT ENELLEQSL RQQNALLRQ ILLERERDL 
      RLETQSLPA GTQTDQDRE TQTEPLFLR PPRRRVRSD 
      NDASDGSDD EISIMRARA RRRRTQRKI KTPIQEESE 
      VEEKPAKSK SITKPLIRQ TRTSELRQK KATALSTKP 
      RNIRKEVLR EISASLEHS DSSEEYYTE DSLDEATPR 
      SEKTTSTRH KYHSESDLR VPSPVSGKP KSQSHSDLT 
      TKPKKKPKT KRTARYMEW YNKSSKTTT SKDTTSVKS 
      EKPPTKEDK LIKETVRKV TSRLMQDTE SSSKKKVDS 
      KKRPTGPEH PLLQHSEHR FEVRYPRKV EDDKDTDSG 
      IALTRLPIA QKKSIFTIA YDDMHTKQL VAQSPPL
    CDS sequence
    • >TC011230 RA
      atgatcaaa gtcgtccta acatggctc acatatctt 
      tccgccgta tcttcaatc ccacaattc gatcccact 
      gccctcctc caaggcatt ttaatccct gccgatgtc 
      gctgtcggc accatcatc tacagactg cgtgcccac 
      gacccaacc ttcgagtac cctctagtc ttttccctc 
      caaaacgaa ccaacaacc gtcagcatc accagcctg 
      aactgcact cgtctcaat tccatttgc caagccaac 
      gtcgttcta agacgaaaa ctaaaagtt gatctcttt 
      tacgacttc aacgtaatt gtacaaaac caaagaggt 
      gaaactgcc ggtctaaat tgctcattt cgagcaacg 
      aacgcaacg acaccaatt aacgagatt tttcccgga 
      gctccgact ttgttgagc gtctctgaa agtgccaga 
      agaaacgcg gaattagga agcataatt gcgaaagga 
      aatcccaat cgaccagat ggtgtccta ctggaatta 
      tggggggca gcacagttt ggactttac caaaaactg 
      atcacggaa aaagacgca caagccact attgttctg 
      ttaggggcg ctggattat gaaacaaca aatgtgcat 
      catctgact atattagct aacgaccct tggactcga 
      atgcaagaa gacacccga aacatcgca agctggtcc 
      ctcctgatc gccgtcatc gacgaacaa gacacccct 
      ccaatcttc accctagcc ccacctaca acaacccta 
      agccccagc cttcttccc ggcgatgtg gtcttgcga 
      atccacgcc gaagatggc gaccgaggc aatcccaga 
      gacatacga tacggtcta gttcccgaa aacaacccc 
      tttacacct ttcttcaac atttcggaa gaaaccggc 
      gaacttcgc ctcgtacga cctttaaac gaaatcgtc 
      gccatttcg cacagcggc caacccatt ctcctgacg 
      gtaattgcc gaagaagtc cgaacaaac ccacaagaa 
      ccctcagcg caatcatca acagtaagt ctagccttg 
      ataccgccc ggtatccgg cccggaaga ccaattttt 
      gggtcaagc gaataccaa actctcctg gacgaaaat 
      tcgcaaagt ggaactgtt ttaaattta ccacaggcc 
      gaagttgtg gcccaacct ggagacgtg gtggcgctg 
      acactagtc ggaaacaac aacactttt gaaattgcc 
      ccaagtgtg gtggaagga caatcaaag tttgaaata 
      cgtgtccat gacccgaaa ctgctagat tacgaaaaa 
      tggcactct gttcaatgc tatatcgtt gcaagggaa 
      gtcgggact gggaatttt acagcacaa gcgaaacta 
      gaagtgtta ctcaacgat gtcaatgac aatgttccc 
      gaattttcg caggaggaa taccaagga acaattcag 
      gaaaacgca cccttcggg acaacactt ctaagggtt 
      gaagcaagt gatagtgac cgagctccc ggtagcaaa 
      atcaagtac gtgcgactt tatggtgac ggaagtgat 
      tttttcagt ttggactca actaccggt cttgtcact 
      gtttccgat ccttcgggt ctagatgct gagaaacac 
      ccaaagttg agtttcttc gttgaagct gcagatgag 
      gagggaaaa gggcaaacg tcaactgct tccataata 
      gttcggttg attgacatc aacgatgaa gcgccacag 
      tttgagaaa aacgtctac gagtttatt ctaaaccat 
      gacaagact gcgttcacg accagagcg tttatcaaa 
      gcagttgac agggatata agtccacca aataacgag 
      attcattac gaattgatc aaaccagtt gaaggtctt 
      cacttgaat gaaagaacg ggggagttg gtgattacg 
      aggacttgg ttggagagt aatattgta attacgacg 
      gcaagggct tgggatggg ggtgttcca agattgttt 
      agtgagtgt gagattagg atttatcca cctgagagt 
      cactcgaga cggatggtt tttattgtg cctgggaaa 
      aatcctgat ttggctgat cttcagaag aaattgagt 
      gtgttgacg ggtgccaag atcacaatt aatgaagtt 
      aggccatat ttgggggcg gagcctgga gccaaagac 
      gtcagcggc tacaattca gaaaaaagt gtggtggtt 
      gccaccgcc acctactcg gacgacttc atcgacgtc 
      aacaccgtc aaacaagtt ttcgacgaa aaacacaca 
      acccaaatt gaaaaagcg tcccaaaac ggtggcaac 
      cttctctgg ctactcatc cttctaata atcctagcc 
      ctcctactg gccattatc ctcatcctg tgctgcatt 
      tgccaagga tgtccttta tacgtcccc ccaaagcgt 
      cgaaaagta gtttccgcc gaaataaaa aaagttgtc 
      cgtggttct ggtaacgga aaagaaagc aaatctgtt 
      caagttgcc gaatggttc ggtcgtcgg gaagcttgg 
      tctcctgaa cacgtcgac aacgaagct gagtcttta 
      caaagacac gaacaggaa agaggttcc gaccgaact 
      gaagccaga cgtacgatt catcgacca ctacaaagg 
      gaaccgtca agggaacag ttttacatc cgtgaagga 
      aacaccgat attttacga ttgataacg cgagggaat 
      gatcaaagg gccgaccaa acttactat ataacagac 
      agtgggaag gatatcctc atgagacgc ttcatagac 
      caacaacaa gccgaaact ttgaaacaa gtcccttta 
      ccaaactca gtcacaaaa ctacaaact gaaaatgaa 
      ctccttgaa caatccttg agacaacaa aacgcttta 
      cttcgccag attttgcta gaacgtgag cgggatttg 
      agattggag actcagtcg cttcctgca ggtactcag 
      acagatcag gatcgggaa acgcaaact gagccactt 
      ttcctgaga ccgcctcga agaagagtt cgctcagat 
      aacgatgcg agtgatgga agtgacgac gaaatctcc 
      ataatgaga gcaagagcc agaaggcga agaacacaa 
      cgaaaaatc aaaacaccg attcaggaa gaatcagaa 
      gttgaggaa aaaccagcc aaaagcaaa agcatcaca 
      aaaccattg attcgccaa acgcgaacc agtgaactc 
      cgccaaaag aaagcaaca gctctttcc acaaaacca 
      agaaatatc cgaaaagaa gtcttgagg gaaatctca 
      gcatcattg gaacatagc gatagcagt gaagaatac 
      tacactgaa gatagccta gatgaagca actccacgt 
      tcagaaaaa acaacatca acacgacac aagtaccac 
      tctgagagc gatcttcgg gtcccttca cccgttagt 
      ggtaagccc aagtcacag agtcactcg gacctgacc 
      accaagccc aaaaagaag cccaaaacc aaacggacg 
      gccaggtac atggaatgg tacaacaaa agttccaaa 
      accacgacc agtaaagac accacaagt gtcaaatcg 
      gaaaaaccc ccaactaaa gaagacaag ctcatcaag 
      gaaacggtc cggaaagtt accagtcgg ttaatgcaa 
      gacactgaa tccagttcg aagaagaaa gttgattcg 
      aaaaaaaga ccaacgggt ccggaacat cctctgttg 
      caacattcg gaacatcgg ttcgaagtg cggtatccg 
      aggaaagtg gaggatgat aaagatacg gattcgggg 
      attgcgttg acgaggctt ccgattgca cagaagaag 
      agtattttt accatcgcc tacgatgat atgcatacg 
      aagcagttg gtggctcag agtccacct ttgtaa
    mRNA sequence
    • >TC011230 RA
      tacctttat taccatacc catacatta taactagcg 
      tcacgaaac tcggatatg taaggggag aaaataccc 
      tactttaag aaacgctag gaaaaacgc tgtgatttt 
      ttacaagac tggccgtat gtgcggtgt taacaatgg 
      atttgttcg ttcgagatg gttttgtgg ttgtacgat 
      tgtgtctta aaattgtga aaaagcttc ccgaaattt 
      taatatttt tgtaatcga tagatttac tgttgttac 
      tacagattt taaatattt atcttcgag gtgcccaaa 
      tttcaatca ggtctggta aacaagaaa atgatcaaa 
      gtcgtccta acatggctc acatatctt tccgccgta 
      tcttcaatc ccacaattc gatcccact gccctcctc 
      caaggcatt ttaatccct gccgatgtc gctgtcggc 
      accatcatc tacagactg cgtgcccac gacccaacc 
      ttcgagtac cctctagtc ttttccctc caaaacgaa 
      ccaacaacc gtcagcatc accagcctg aactgcact 
      cgtctcaat tccatttgc caagccaac gtcgttcta 
      agacgaaaa ctaaaagtt gatctcttt tacgacttc 
      aacgtaatt gtacaaaac caaagaggt gaaactgcc 
      ggtctaaat tgctcattt cgagcaacg aacgcaacg 
      acaccaatt aacgagatt tttcccgga gctccgact 
      ttgttgagc gtctctgaa agtgccaga agaaacgcg 
      gaattagga agcataatt gcgaaagga aatcccaat 
      cgaccagat ggtgtccta ctggaatta tggggggca 
      gcacagttt ggactttac caaaaactg atcacggaa 
      aaagacgca caagccact attgttctg ttaggggcg 
      ctggattat gaaacaaca aatgtgcat catctgact 
      atattagct aacgaccct tggactcga atgcaagaa 
      gacacccga aacatcgca agctggtcc ctcctgatc 
      gccgtcatc gacgaacaa gacacccct ccaatcttc 
      accctagcc ccacctaca acaacccta agccccagc 
      cttcttccc ggcgatgtg gtcttgcga atccacgcc 
      gaagatggc gaccgaggc aatcccaga gacatacga 
      tacggtcta gttcccgaa aacaacccc tttacacct 
      ttcttcaac atttcggaa gaaaccggc gaacttcgc 
      ctcgtacga cctttaaac gaaatcgtc gccatttcg 
      cacagcggc caacccatt ctcctgacg gtaattgcc 
      gaagaagtc cgaacaaac ccacaagaa ccctcagcg 
      caatcatca acagtaagt ctagccttg ataccgccc 
      ggtatccgg cccggaaga ccaattttt gggtcaagc 
      gaataccaa actctcctg gacgaaaat tcgcaaagt 
      ggaactgtt ttaaattta ccacaggcc gaagttgtg 
      gcccaacct ggagacgtg gtggcgctg acactagtc 
      ggaaacaac aacactttt gaaattgcc ccaagtgtg 
      gtggaagga caatcaaag tttgaaata cgtgtccat 
      gacccgaaa ctgctagat tacgaaaaa tggcactct 
      gttcaatgc tatatcgtt gcaagggaa gtcgggact 
      gggaatttt acagcacaa gcgaaacta gaagtgtta 
      ctcaacgat gtcaatgac aatgttccc gaattttcg 
      caggaggaa taccaagga acaattcag gaaaacgca 
      cccttcggg acaacactt ctaagggtt gaagcaagt 
      gatagtgac cgagctccc ggtagcaaa atcaagtac 
      gtgcgactt tatggtgac ggaagtgat tttttcagt 
      ttggactca actaccggt cttgtcact gtttccgat 
      ccttcgggt ctagatgct gagaaacac ccaaagttg 
      agtttcttc gttgaagct gcagatgag gagggaaaa 
      gggcaaacg tcaactgct tccataata gttcggttg 
      attgacatc aacgatgaa gcgccacag tttgagaaa 
      aacgtctac gagtttatt ctaaaccat gacaagact 
      gcgttcacg accagagcg tttatcaaa gcagttgac 
      agggatata agtccacca aataacgag attcattac 
      gaattgatc aaaccagtt gaaggtctt cacttgaat 
      gaaagaacg ggggagttg gtgattacg aggacttgg 
      ttggagagt aatattgta attacgacg gcaagggct 
      tgggatggg ggtgttcca agattgttt agtgagtgt 
      gagattagg atttatcca cctgagagt cactcgaga 
      cggatggtt tttattgtg cctgggaaa aatcctgat 
      ttggctgat cttcagaag aaattgagt gtgttgacg 
      ggtgccaag atcacaatt aatgaagtt aggccatat 
      ttgggggcg gagcctgga gccaaagac gtcagcggc 
      tacaattca gaaaaaagt gtggtggtt gccaccgcc 
      acctactcg gacgacttc atcgacgtc aacaccgtc 
      aaacaagtt ttcgacgaa aaacacaca acccaaatt 
      gaaaaagcg tcccaaaac ggtggcaac cttctctgg 
      ctactcatc cttctaata atcctagcc ctcctactg 
      gccattatc ctcatcctg tgctgcatt tgccaagga 
      tgtccttta tacgtcccc ccaaagcgt cgaaaagta 
      gtttccgcc gaaataaaa aaagttgtc cgtggttct 
      ggtaacgga aaagaaagc aaatctgtt caagttgcc 
      gaatggttc ggtcgtcgg gaagcttgg tctcctgaa 
      cacgtcgac aacgaagct gagtcttta caaagacac 
      gaacaggaa agaggttcc gaccgaact gaagccaga 
      cgtacgatt catcgacca ctacaaagg gaaccgtca 
      agggaacag ttttacatc cgtgaagga aacaccgat 
      attttacga ttgataacg cgagggaat gatcaaagg 
      gccgaccaa acttactat ataacagac agtgggaag 
      gatatcctc atgagacgc ttcatagac caacaacaa 
      gccgaaact ttgaaacaa gtcccttta ccaaactca 
      gtcacaaaa ctacaaact gaaaatgaa ctccttgaa 
      caatccttg agacaacaa aacgcttta cttcgccag 
      attttgcta gaacgtgag cgggatttg agattggag 
      actcagtcg cttcctgca ggtactcag acagatcag 
      gatcgggaa acgcaaact gagccactt ttcctgaga 
      ccgcctcga agaagagtt cgctcagat aacgatgcg 
      agtgatgga agtgacgac gaaatctcc ataatgaga 
      gcaagagcc agaaggcga agaacacaa cgaaaaatc 
      aaaacaccg attcaggaa gaatcagaa gttgaggaa 
      aaaccagcc aaaagcaaa agcatcaca aaaccattg 
      attcgccaa acgcgaacc agtgaactc cgccaaaag 
      aaagcaaca gctctttcc acaaaacca agaaatatc 
      cgaaaagaa gtcttgagg gaaatctca gcatcattg 
      gaacatagc gatagcagt gaagaatac tacactgaa 
      gatagccta gatgaagca actccacgt tcagaaaaa 
      acaacatca acacgacac aagtaccac tctgagagc 
      gatcttcgg gtcccttca cccgttagt ggtaagccc 
      aagtcacag agtcactcg gacctgacc accaagccc 
      aaaaagaag cccaaaacc aaacggacg gccaggtac 
      atggaatgg tacaacaaa agttccaaa accacgacc 
      agtaaagac accacaagt gtcaaatcg gaaaaaccc 
      ccaactaaa gaagacaag ctcatcaag gaaacggtc 
      cggaaagtt accagtcgg ttaatgcaa gacactgaa 
      tccagttcg aagaagaaa gttgattcg aaaaaaaga 
      ccaacgggt ccggaacat cctctgttg caacattcg 
      gaacatcgg ttcgaagtg cggtatccg aggaaagtg 
      gaggatgat aaagatacg gattcgggg attgcgttg 
      acgaggctt ccgattgca cagaagaag agtattttt 
      accatcgcc tacgatgat atgcatacg aagcagttg 
      gtggctcag agtccacct ttgtaattt ttgtacgtt 
      tagttcatt gaccgcgat tttcttatc tcatgtaaa 
      ggattaatt aaataatga ccagtaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY