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Gene information
General information
TC011228 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 819721..833032
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011228
    iBeetle screen iB_04995

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 8 (16 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011228 RA
      MPPKKKGGK GKGGSGNII DGVDTTQMT REQLEVFCH 
      RIKEENERE REERNFFQL ERDKLRTFW EITKNELEE 
      AKAKLRNKD RQIEEAAEK NEEELKFYK QKVKHLQYE 
      HQNNLTECK AEALVSLKM SQDDHTEQE RELLRDKRD 
      LKAQMREQE VAHQDQVKA LKLQHSEEI STIRNQFEL 
      KEKELENKY EKKFADLKQ ELNIKHTME MSELEERKN 
      NQITDLTKH HEKVFNEMK NYYNDITLN NLALISSLK 
      DQMEVLRKQ NERMSKQVA DITAENKRL TEPLKQAQI 
      DVAEYKRQL EHYEKDKLS LANTKAKLS STKQELDDL 
      RWANDALQL RFEKLEAEK DELSRRFIE AILDVQQKT 
      GQKNMLLQK RIQTLTNVA EYRDVIIGE LRALTSEDI 
      TAKNLKLEK ILARKNTVI HDLQYELAR VCKAHDDLL 
      DTYEDKLQQ YGIPKEELG FVPLRILQD GQGGLPKGP 
      AGLVTKNR
    CDS sequence
    • >TC011228 RA
      atgcctccc aaaaagaaa ggtggaaaa ggaaaaggc 
      ggttcggga aatatcatc gatggagtg gacacaact 
      caaatgacc cgagagcag ctcgaggtc ttctgccac 
      cgaataaaa gaggaaaac gaacgagaa agagaagaa 
      cgaaatttc tttcaactc gaaagagat aaattacgg 
      acgttttgg gaaatcaca aaaaatgaa cttgaagaa 
      gctaaagca aaactcaga aacaaagac cgacagatt 
      gaggaagcg gctgagaaa aacgaagaa gaactgaaa 
      ttttacaag caaaaagtc aaacatttg caatacgaa 
      caccaaaac aacttaaca gaatgtaaa gctgaagcg 
      cttgtttcg ctgaaaatg tcacaagat gaccacact 
      gaacaggaa agagaattg ttgcgagat aagcgagat 
      ttgaaagcg caaatgcgg gaacaagaa gtcgcccat 
      caagatcaa gtcaaagct ttaaaactg caacatagt 
      gaagagata agcaccatc agaaaccaa ttcgaactg 
      aaagaaaaa gaactggaa aataaatac gaaaaaaaa 
      tttgctgac ctgaaacaa gaattaaac atcaaacat 
      acgatggaa atgtcggag ctggaagag cgcaaaaat 
      aaccaaatt actgatttg acaaaacat cacgagaaa 
      gtgttcaat gaaatgaag aactattac aatgacatt 
      actttaaac aacttggcg ttaataagt agcttgaaa 
      gaccaaatg gaagttctt cggaaacaa aacgaacga 
      atgagtaaa caagtcgct gacataact gccgaaaac 
      aagcgctta actgaacct ttaaaacaa gcacaaatt 
      gatgttgct gagtataaa cgacaactt gaacattac 
      gaaaaggac aagttatca ctggcgaac accaaagct 
      aaattatcg tccactaaa caagagctg gatgattta 
      agatgggca aacgatgct ttgcaatta agatttgaa 
      aagctcgag gccgaaaaa gacgaactg agtcgaaga 
      ttcatcgag gcaattcta gacgttcag cagaaaacc 
      ggtcagaaa aatatgctt ttgcagaaa cgtatccaa 
      actttgaca aatgtggcg gaatacaga gatgtgata 
      attggggag ctgagggcg ctaacgtcg gaagatatt 
      acggcgaaa aatttgaag ctggagaaa attttagca 
      agaaaaaat acggtcata catgactta cagtatgag 
      ctggccagg gtgtgtaaa gcgcacgat gatcttttg 
      gatacttac gaagataaa ttgcaacaa tatggcatt 
      cctaaagag gagttgggt tttgttccg ttgaggatt 
      ttgcaggat gggcaaggg gggctgccg aaaggccca 
      gctgggctt gttacaaag aatcgataa
    mRNA sequence
    • >TC011228 RA
      ttaatagtg agtgagtgt attgtcatc tgcaattac 
      cgttctaaa atcaaattg aaattcaag cagtgtcat 
      tgactcatt gggcagaat tctccctta aaataagtc 
      cattccaaa gaaatcgat acgcgggta gtaaccatc 
      taaacaaat agtttcttg aagaactgc tagctagtt 
      cattcactt tcacttcgt aaaatgcct cccaaaaag 
      aaaggtgga aaaggaaaa ggcggttcg ggaaatatc 
      atcgatgga gtggacaca actcaaatg acccgagag 
      cagctcgag gtcttctgc caccgaata aaagaggaa 
      aacgaacga gaaagagaa gaacgaaat ttctttcaa 
      ctcgaaaga gataaatta cggacgttt tgggaaatc 
      acaaaaaat gaacttgaa gaagctaaa gcaaaactc 
      agaaacaaa gaccgacag attgaggaa gcggctgag 
      aaaaacgaa gaagaactg aaattttac aagcaaaaa 
      gtcaaacat ttgcaatac gaacaccaa aacaactta 
      acagaatgt aaagctgaa gcgcttgtt tcgctgaaa 
      atgtcacaa gatgaccac actgaacag gaaagagaa 
      ttgttgcga gataagcga gatttgaaa gcgcaaatg 
      cgggaacaa gaagtcgcc catcaagat caagtcaaa 
      gctttaaaa ctgcaacat agtgaagag ataagcacc 
      atcagaaac caattcgaa ctgaaagaa aaagaactg 
      gaaaataaa tacgaaaaa aaatttgct gacctgaaa 
      caagaatta aacatcaaa catacgatg gaaatgtcg 
      gagctggaa gagcgcaaa aataaccaa attactgat 
      ttgacaaaa catcacgag aaagtgttc aatgaaatg 
      aagaactat tacaatgac attacttta aacaacttg 
      gcgttaata agtagcttg aaagaccaa atggaagtt 
      cttcggaaa caaaacgaa cgaatgagt aaacaagtc 
      gctgacata actgccgaa aacaagcgc ttaactgaa 
      cctttaaaa caagcacaa attgatgtt gctgagtat 
      aaacgacaa cttgaacat tacgaaaag gacaagtta 
      tcactggcg aacaccaaa gctaaatta tcgtccact 
      aaacaagag ctggatgat ttaagatgg gcaaacgat 
      gctttgcaa ttaagattt gaaaagctc gaggccgaa 
      aaagacgaa ctgagtcga agattcatc gaggcaatt 
      ctagacgtt cagcagaaa accggtcag aaaaatatg 
      cttttgcag aaacgtatc caaactttg acaaatgtg 
      gcggaatac agagatgtg ataattggg gagctgagg 
      gcgctaacg tcggaagat attacggcg aaaaatttg 
      aagctggag aaaatttta gcaagaaaa aatacggtc 
      atacatgac ttacagtat gagctggcc agggtgtgt 
      aaagcgcac gatgatctt ttggatact tacgaagat 
      aaattgcaa caatatggc attcctaaa gaggagttg 
      ggttttgtt ccgttgagg attttgcag gatgggcaa 
      ggggggctg ccgaaaggc ccagctggg cttgttaca 
      aagaatcga taattgtgt gaataaatg gcgaaatta 
      aactgtttt attttta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY