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Gene information
General information
TC011225 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 1081432..1090159
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011225
    iBeetle screen iB_04992

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011225 RA
      MATSSAASK QSQAPAGVK EYTIKVPKN VQKRYHVMR 
      FNGNLEVDF AKWTQVKME RENNLKEYK SVEEEQPKF 
      GAGSEFGRE AKEEARRKK LGIVARKYR PEDQPWILK 
      SYGSTVKKF KGIREGGVS ENTAYYVFT HAADGAIEA 
      FPLHEWYKF QPIQRYKAL TAEEAEQEF NKRNKHLNY 
      FSLMLRKRL KGEDDTELD EGEEKGKKN AKKSKELKI 
      SDMDEWMDS SDDEGSEEE EKEEEEEKK EKKKKKGKK 
      VDPKKKKRD TDVEAFEDS DDGDGEGRE LDYISDSSE 
      SEPENTNLE SVAEEKALR KLLTSDEDS ENDSDKSEK 
      EQSNEDEEN KEKESEKTE EGADKDKKK KNKKKNKKE 
      IKKEVKKEK ESNSDFSSD SSTDEETKK KKNDKKMKK 
      EEREANSAN SSRSATPTD HKRKLVNDV MAGPSAKKQ 
      KLDLPNYIV GSSESGITE DAVRKYLMR KPMTTTELL 
      QKFKSKKTG LSSEQLVNI MTQILKKIN PVKQTLKGK 
      MYLSIKS
    • >TC011225 RB
      MATSSAASQ KQSQAPAGV KEYTIKVPK NVQKRYHVM 
      RFNGNLEVD FAKWTQVKM ERENNLKEY KSVEEEQPK 
      FGAGSEFGR EAKEEARRK KLGIVARKY RPEDQPWIL 
      KSYGSTVKK FKGIREGGV SENTAYYVF THAADGAIE 
      AFPLHEWYK FQPIQRYKA LTAEEAEQE FNKRNKHLN 
      YFSLMLRKR LKGEDDTEL DEGEEKGKK NAKKSKELK 
      ISDMDEWMD SSDDEGSEE EEKEEEEEK KEKKKKKGK 
      KVDPKKKKR DTDVEAFED SDDGDGEGR ELDYISDSS 
      ESEPENTNL ESVAEEKAL RKLLTSDED SENDSDKSE 
      KEQSNEDEE NKEKESEKT EEGADKDKK KKNKKKNKK 
      EIKKEVKKE KESNSDFSS DSSTDEETK KKKNDKKMK 
      KEEREANSA NSSRSATPT DHKRKLVND VMAGPSAKK 
      QKLDLPNYI VGSSESGIT EDAVRKYLM RKPMTTTEL 
      LQKFKSKKT GLSSEQLVN IMTQILKKI NPVKQTLKG 
      KMYLSIKS
    CDS sequence
    • >TC011225 RA
      atggcgaca agttcagcc gcctctaag caatcacag 
      gccccagcc ggtgttaag gagtataca atcaaagta 
      ccgaaaaac gtccagaag cgataccat gtaatgaga 
      tttaatgga aatcttgaa gtggatttt gccaagtgg 
      acgcaggtc aaaatggaa agggagaat aatttgaaa 
      gagtacaaa agcgtcgaa gaagaacaa ccaaagttt 
      ggcgcaggt tcggagttt ggccgcgag gccaaagag 
      gaagcccgt cgcaaaaaa ctgggaata gttgctaga 
      aaataccga cctgaggac cagccttgg attttgaag 
      tcttacgga agcactgtg aaaaaattc aaggggatt 
      cgagaaggc ggtgtttct gaaaacaca gcctactat 
      gttttcact catgctgct gatggtgca attgaggct 
      tttccgtta cacgaatgg tacaaattt caaccaatt 
      caaagatac aaagctctg acagctgaa gaagccgaa 
      caagagttc aataaacgc aataaacat ttgaactat 
      ttttcactt atgttgagg aagagactg aaaggtgag 
      gatgatact gagcttgat gaaggagag gaaaaaggg 
      aaaaagaat gcaaagaaa tcgaaggaa ttgaaaata 
      tcggatatg gacgagtgg atggatagt tcagatgat 
      gaagggtct gaggaagag gagaaggaa gaggaagaa 
      gagaagaaa gagaagaag aagaaaaaa ggaaagaaa 
      gttgacccg aaaaagaag aagcgggac acagatgtg 
      gaagcattt gaagattcg gatgatgga gatggcgag 
      ggccgagaa ttggattac attagtgat agttcagag 
      agtgaacct gaaaacacg aatctggag agcgtggct 
      gaagaaaaa gccttgcga aaactacta acatcagat 
      gaagattcg gaaaacgat tcagacaaa tccgagaag 
      gaacaaagc aatgaagat gaagaaaac aaagagaaa 
      gaaagcgag aaaaccgag gagggcgct gacaaggac 
      aagaagaag aagaacaaa aagaagaac aagaaagag 
      attaaaaag gaggtgaag aaagagaaa gagtcaaac 
      agtgatttt agttccgat agcagcaca gacgaagag 
      accaaaaag aagaagaat gataagaaa atgaaaaaa 
      gaagagagg gaagcaaat agtgccaat agttcaagg 
      tctgcaacg ccaactgat cacaagcgg aagttagta 
      aatgatgta atggcagga ccgtctgct aaaaaacag 
      aagttagat ttaccgaat tatattgtt gggtcgagt 
      gagtctgga attaccgaa gatgctgtt agaaaatat 
      ctaatgagg aagccgatg acaaccact gagcttttg 
      cagaaattc aaatcgaaa aagacggga ctttcgagc 
      gaacagctt gtcaatatt atgacacaa atattgaag 
      aaaattaat cccgtgaaa caaacattg aaagggaag 
      atgtatctg tcgataaag tcttga
    • >TC011225 RB
      atggcgaca agttcagcc gcctctcag aagcaatca 
      caggcccca gccggtgtt aaggagtat acaatcaaa 
      gtaccgaaa aacgtccag aagcgatac catgtaatg 
      agatttaat ggaaatctt gaagtggat tttgccaag 
      tggacgcag gtcaaaatg gaaagggag aataatttg 
      aaagagtac aaaagcgtc gaagaagaa caaccaaag 
      tttggcgca ggttcggag tttggccgc gaggccaaa 
      gaggaagcc cgtcgcaaa aaactggga atagttgct 
      agaaaatac cgacctgag gaccagcct tggattttg 
      aagtcttac ggaagcact gtgaaaaaa ttcaagggg 
      attcgagaa ggcggtgtt tctgaaaac acagcctac 
      tatgttttc actcatgct gctgatggt gcaattgag 
      gcttttccg ttacacgaa tggtacaaa tttcaacca 
      attcaaaga tacaaagct ctgacagct gaagaagcc 
      gaacaagag ttcaataaa cgcaataaa catttgaac 
      tatttttca cttatgttg aggaagaga ctgaaaggt 
      gaggatgat actgagctt gatgaagga gaggaaaaa 
      gggaaaaag aatgcaaag aaatcgaag gaattgaaa 
      atatcggat atggacgag tggatggat agttcagat 
      gatgaaggg tctgaggaa gaggagaag gaagaggaa 
      gaagagaag aaagagaag aagaagaaa aaaggaaag 
      aaagttgac ccgaaaaag aagaagcgg gacacagat 
      gtggaagca tttgaagat tcggatgat ggagatggc 
      gagggccga gaattggat tacattagt gatagttca 
      gagagtgaa cctgaaaac acgaatctg gagagcgtg 
      gctgaagaa aaagccttg cgaaaacta ctaacatca 
      gatgaagat tcggaaaac gattcagac aaatccgag 
      aaggaacaa agcaatgaa gatgaagaa aacaaagag 
      aaagaaagc gagaaaacc gaggagggc gctgacaag 
      gacaagaag aagaagaac aaaaagaag aacaagaaa 
      gagattaaa aaggaggtg aagaaagag aaagagtca 
      aacagtgat tttagttcc gatagcagc acagacgaa 
      gagaccaaa aagaagaag aatgataag aaaatgaaa 
      aaagaagag agggaagca aatagtgcc aatagttca 
      aggtctgca acgccaact gatcacaag cggaagtta 
      gtaaatgat gtaatggca ggaccgtct gctaaaaaa 
      cagaagtta gatttaccg aattatatt gttgggtcg 
      agtgagtct ggaattacc gaagatgct gttagaaaa 
      tatctaatg aggaagccg atgacaacc actgagctt 
      ttgcagaaa ttcaaatcg aaaaagacg ggactttcg 
      agcgaacag cttgtcaat attatgaca caaatattg 
      aagaaaatt aatcccgtg aaacaaaca ttgaaaggg 
      aagatgtat ctgtcgata aagtcttga
    mRNA sequence
    • >TC011225 RA
      ccgacatgg tgacctaac ctctaaacg aacttgttt 
      gacagctgt tttctaaat aggtctatt ttttaattt 
      gagtttaat ttaactaat aaattttgc aattaattg 
      taatttggt taaaatggc gacaagttc agccgcctc 
      taagcaatc acaggcccc agccggtgt taaggagta 
      tacaatcaa agtaccgaa aaacgtcca gaagcgata 
      ccatgtaat gagatttaa tggaaatct tgaagtgga 
      ttttgccaa gtggacgca ggtcaaaat ggaaaggga 
      gaataattt gaaagagta caaaagcgt cgaagaaga 
      acaaccaaa gtttggcgc aggttcgga gtttggccg 
      cgaggccaa agaggaagc ccgtcgcaa aaaactggg 
      aatagttgc tagaaaata ccgacctga ggaccagcc 
      ttggatttt gaagtctta cggaagcac tgtgaaaaa 
      attcaaggg gattcgaga aggcggtgt ttctgaaaa 
      cacagccta ctatgtttt cactcatgc tgctgatgg 
      tgcaattga ggcttttcc gttacacga atggtacaa 
      atttcaacc aattcaaag atacaaagc tctgacagc 
      tgaagaagc cgaacaaga gttcaataa acgcaataa 
      acatttgaa ctatttttc acttatgtt gaggaagag 
      actgaaagg tgaggatga tactgagct tgatgaagg 
      agaggaaaa agggaaaaa gaatgcaaa gaaatcgaa 
      ggaattgaa aatatcgga tatggacga gtggatgga 
      tagttcaga tgatgaagg gtctgagga agaggagaa 
      ggaagagga agaagagaa gaaagagaa gaagaagaa 
      aaaaggaaa gaaagttga cccgaaaaa gaagaagcg 
      ggacacaga tgtggaagc atttgaaga ttcggatga 
      tggagatgg cgagggccg agaattgga ttacattag 
      tgatagttc agagagtga acctgaaaa cacgaatct 
      ggagagcgt ggctgaaga aaaagcctt gcgaaaact 
      actaacatc agatgaaga ttcggaaaa cgattcaga 
      caaatccga gaaggaaca aagcaatga agatgaaga 
      aaacaaaga gaaagaaag cgagaaaac cgaggaggg 
      cgctgacaa ggacaagaa gaagaagaa caaaaagaa 
      gaacaagaa agagattaa aaaggaggt gaagaaaga 
      gaaagagtc aaacagtga ttttagttc cgatagcag 
      cacagacga agagaccaa aaagaagaa gaatgataa 
      gaaaatgaa aaaagaaga gagggaagc aaatagtgc 
      caatagttc aaggtctgc aacgccaac tgatcacaa 
      gcggaagtt agtaaatga tgtaatggc aggaccgtc 
      tgctaaaaa acagaagtt agatttacc gaattatat 
      tgttgggtc gagtgagtc tggaattac cgaagatgc 
      tgttagaaa atatctaat gaggaagcc gatgacaac 
      cactgagct tttgcagaa attcaaatc gaaaaagac 
      gggactttc gagcgaaca gcttgtcaa tattatgac 
      acaaatatt gaagaaaat taatcccgt gaaacaaac 
      attgaaagg gaagatgta tctgtcgat aaagtcttg 
      attgaatca ataatgttg ctgtaaaaa ttttctatc 
      tataaggag ttaaaggag tatgatatt tatgtataa 
      tgggagttg taattacat attaattaa taaaaattt 
      atttacagc
    • >TC011225 RB
      ccgacatgg tgacctaac ctctaaacg aacttgttt 
      gacagctgt tttctaaat aggtctatt ttttaattt 
      gagtttaat ttaactaat aaattttgc aattaattg 
      taatttggt taaaatggc gacaagttc agccgcctc 
      tcagaagca atcacaggc cccagccgg tgttaagga 
      gtatacaat caaagtacc gaaaaacgt ccagaagcg 
      ataccatgt aatgagatt taatggaaa tcttgaagt 
      ggattttgc caagtggac gcaggtcaa aatggaaag 
      ggagaataa tttgaaaga gtacaaaag cgtcgaaga 
      agaacaacc aaagtttgg cgcaggttc ggagtttgg 
      ccgcgaggc caaagagga agcccgtcg caaaaaact 
      gggaatagt tgctagaaa ataccgacc tgaggacca 
      gccttggat tttgaagtc ttacggaag cactgtgaa 
      aaaattcaa ggggattcg agaaggcgg tgtttctga 
      aaacacagc ctactatgt tttcactca tgctgctga 
      tggtgcaat tgaggcttt tccgttaca cgaatggta 
      caaatttca accaattca aagatacaa agctctgac 
      agctgaaga agccgaaca agagttcaa taaacgcaa 
      taaacattt gaactattt ttcacttat gttgaggaa 
      gagactgaa aggtgagga tgatactga gcttgatga 
      aggagagga aaaagggaa aaagaatgc aaagaaatc 
      gaaggaatt gaaaatatc ggatatgga cgagtggat 
      ggatagttc agatgatga agggtctga ggaagagga 
      gaaggaaga ggaagaaga gaagaaaga gaagaagaa 
      gaaaaaagg aaagaaagt tgacccgaa aaagaagaa 
      gcgggacac agatgtgga agcatttga agattcgga 
      tgatggaga tggcgaggg ccgagaatt ggattacat 
      tagtgatag ttcagagag tgaacctga aaacacgaa 
      tctggagag cgtggctga agaaaaagc cttgcgaaa 
      actactaac atcagatga agattcgga aaacgattc 
      agacaaatc cgagaagga acaaagcaa tgaagatga 
      agaaaacaa agagaaaga aagcgagaa aaccgagga 
      gggcgctga caaggacaa gaagaagaa gaacaaaaa 
      gaagaacaa gaaagagat taaaaagga ggtgaagaa 
      agagaaaga gtcaaacag tgattttag ttccgatag 
      cagcacaga cgaagagac caaaaagaa gaagaatga 
      taagaaaat gaaaaaaga agagaggga agcaaatag 
      tgccaatag ttcaaggtc tgcaacgcc aactgatca 
      caagcggaa gttagtaaa tgatgtaat ggcaggacc 
      gtctgctaa aaaacagaa gttagattt accgaatta 
      tattgttgg gtcgagtga gtctggaat taccgaaga 
      tgctgttag aaaatatct aatgaggaa gccgatgac 
      aaccactga gcttttgca gaaattcaa atcgaaaaa 
      gacgggact ttcgagcga acagcttgt caatattat 
      gacacaaat attgaagaa aattaatcc cgtgaaaca 
      aacattgaa agggaagat gtatctgtc gataaagtc 
      ttgattgaa tcaataatg ttgctgtaa aaattttct 
      atctataag gagttaaag gagtatgat atttatgta 
      taatgggag ttgtaatta catattaat taataaaaa 
      tttatttac agc
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