Status
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Gene information
General information
TC031335
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011222
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 1168789..1242694
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031335
    iBeetle screen iB_04991

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031335 RA
      MIFNRGIPL SWWLTILQI NLGLTIQYT PVDLTGNGD 
      GECALLLSG PPRSSHFDY SLNLLRGSP LEYNGPHTC 
      ITYDAVNAA YLDARKRIH IAQPHGDWK PEDVATVGE 
      LLLDISINL ARTYGLSYE DIEKGLPLI DTSKTLIRE 
      VCPPYLSNV ECRAGKYRR FDGLCNNLE HPTWGATNT 
      PFTRLIGPL YADGMTAPR ISVTGRNLP LSRVVSRTM 
      HPDEGFHDH AGTVMVIAW GQFIDHDYT LTATPLDPL 
      NRNDPEECC HRPPHLKHP YCNEIIIPE DDYFYRLFN 
      VKCLDFVRA FPAARPGCR LGSRVPYNT LTGVLDGNT 
      VYGVTEKFA RKLRTGYGG LLRMNPAFE EYGLKDLLP 
      LKLDIPDEG CTRPNSTMY CFEAGEIRV NEQLVLTCM 
      HTLMAREHN RIAKGLAQV NPHWDDETL FQEARRINI 
      AEIQHITYN EFLPILLGK DVMAKFGLL LQKDGYWDG 
      YDHNVNPNV IDAFAAAAY RFGHSLLPT AVERWSKAH 
      KFIASKRLS DLIRRPYDL YRAGVFDEY LMGLMNQVA 
      QAMDDSITQ EVTNHLFKK VGARFGMDL VSLNMQRGR 
      EFGIPGYME FRKFCGLPG ASNFDELFG SMPNETVRK 
      YSTIFEHPS DVDLWSGGV SERPLPGSM LGPTFACII 
      ATQFSYSRR GDRFWYELP NQPSSFTPE QLHEVRKAR 
      LARIICDNT DLIDTIQLY PMVLPDHEI NPRVPCRSG 
      VIPSIDFSK WAEFPHAGF NSFDHVLGK K
    CDS sequence
    • >TC031335 RA
      atgattttt aacaggggg attcccctg agttggtgg 
      ctcacaata ctccaaatc aacctaggc ttaactata 
      caatacact ccagtggac ctcacggga aatggagat 
      ggggaatgt gcccttctt ctaagtggt cctcctaga 
      agtagtcac tttgactac tccctcaac ttactcaga 
      ggaagtccg ttagaatat aatggtcca cacacttgc 
      atcacctac gacgctgtc aatgctgcg tatcttgat 
      gcccgcaaa aggatacac attgctcaa ccacatggc 
      gactggaag cctgaagat gtggcaact gttggtgaa 
      cttcttctg gacatttcg ataaatctg gcacgaacg 
      tacggtttg agttatgaa gacatcgag aaaggtctc 
      cctctgatt gacactagc aagactttg atccgtgag 
      gtgtgcccc ccttatttg tccaatgtg gaatgtcgt 
      gctggcaaa taccgacga tttgatggc ctttgtaac 
      aatttggag cacccgact tggggggcc accaacact 
      ccgtttaca agactgatc ggcccctta tatgccgac 
      ggcatgaca gccccccgc atcagcgta accggaaga 
      aaccttcct ctatcccgt gtcgtctcc cgcactatg 
      catccagat gaaggtttc cacgatcac gccggtacc 
      gtcatggtc attgcatgg ggacaattt atcgaccat 
      gactacact ctcactgca actccatta gaccctcta 
      aatcgaaac gaccccgaa gagtgctgc cacagaccg 
      ccccacctt aaacacccc tactgtaac gaaataatc 
      atccccgaa gacgattac ttctaccga ttattcaac 
      gtaaaatgc ttggacttt gttcgtgct ttcccggcc 
      gctagacct ggttgtaga ctcggttcc cgagttcca 
      tacaacact ttaactggt gttctcgac ggtaacacc 
      gtatacgga gttacggaa aaattcgca cggaaatta 
      agaaccgga tacggtggt cttctccga atgaaccca 
      gcctttgaa gagtacgga cttaaagac ttgctccct 
      ctcaagcta gacatccct gatgaaggg tgcactaga 
      cccaatagc accatgtac tgctttgaa gccggtgaa 
      attcgagtg aacgagcag ttggtgctc acttgtatg 
      cacactctg atggctcgt gagcacaac cgaattgcc 
      aaaggtttg gctcaggtt aatcctcac tgggatgac 
      gaaacgctg ttccaagaa gctcgtcgc attaacatt 
      gccgaaatt caacacatt acttacaac gagtttctt 
      ccgattctt ttgggcaaa gatgtgatg gctaagttt 
      ggtttgctg ttgcagaaa gacggttat tgggatggt 
      tatgatcat aatgttaat ccgaatgtg attgatgcg 
      ttcgcagct gcggcttac agatttggg cactcgctt 
      cttccaacg gctgtggaa aggtggagc aaggcacac 
      aaatttatt gcctccaaa cgtctctct gatttaatc 
      cgtcgcccc tacgactta taccgtgcc ggcgtcttc 
      gacgagtac ctcatgggt ctgatgaac caagtggcc 
      caagccatg gacgactcc atcacccaa gaggtgacc 
      aaccatctg ttcaaaaaa gtcggcgct cggttcggt 
      atggacttg gtcagtctc aacatgcaa agaggccgc 
      gaattcggc attccgggc tacatggaa ttccggaaa 
      ttctgcggc ctgcccgga gccagcaac tttgacgaa 
      ttgttcggt tccatgccc aacgaaact gtccggaaa 
      tactcgacc atttttgag cacccgagt gatgtcgat 
      ttgtggtcg ggcggagtc tccgaacgc ccccttcct 
      ggaagcatg ttagggccc acttttgcc tgcattatc 
      gccacccag tttagttac tcgaggcga ggtgaccgc 
      ttctggtat gaacttcca aaccaaccc tcatctttc 
      acccctgaa caactccac gaagtcagg aaagccaga 
      ttggctcgt attatatgt gacaacacc gatttgatt 
      gacaccata cagttgtac ccaatggtg ttgccggac 
      catgaaatt aacccgagg gtcccgtgc cgaagtgga 
      gtgatcccg agcatagat ttcagcaaa tgggccgaa 
      ttcccgcac gcaggcttc aattctttt gaccacgtg 
      ttaggaaaa aaataa
    mRNA sequence
    • >TC031335 RA
      ttttaataa aaagttgtt ttatctaat tacaaaatt 
      acataaaaa ctgaaagaa atatagacc ctgacaccg 
      gttactcta tcatcgagc catggaggg gtaaaaatt 
      ctctctcct ccttagtgc caaagcttt ggttctcgg 
      caccagctg gtttaataa gtgacagtg tatacgatt 
      gaccgaccg tactaaccc acatccgtg ctcttctcg 
      tgaaagaag tattgtaca acgcgcggg tggagtgca 
      ctgtgcagt gctgcaaac tgttttttt ttctttctt 
      tttcaagtg tgccatagg aacttaata atgattttt 
      aacaggggg attcccctg agttggtgg ctcacaata 
      ctccaaatc aacctaggc ttaactata caatacact 
      ccagtggac ctcacggga aatggagat ggggaatgt 
      gcccttctt ctaagtggt cctcctaga agtagtcac 
      tttgactac tccctcaac ttactcaga ggaagtccg 
      ttagaatat aatggtcca cacacttgc atcacctac 
      gacgctgtc aatgctgcg tatcttgat gcccgcaaa 
      aggatacac attgctcaa ccacatggc gactggaag 
      cctgaagat gtggcaact gttggtgaa cttcttctg 
      gacatttcg ataaatctg gcacgaacg tacggtttg 
      agttatgaa gacatcgag aaaggtctc cctctgatt 
      gacactagc aagactttg atccgtgag gtgtgcccc 
      ccttatttg tccaatgtg gaatgtcgt gctggcaaa 
      taccgacga tttgatggc ctttgtaac aatttggag 
      cacccgact tggggggcc accaacact ccgtttaca 
      agactgatc ggcccctta tatgccgac ggcatgaca 
      gccccccgc atcagcgta accggaaga aaccttcct 
      ctatcccgt gtcgtctcc cgcactatg catccagat 
      gaaggtttc cacgatcac gccggtacc gtcatggtc 
      attgcatgg ggacaattt atcgaccat gactacact 
      ctcactgca actccatta gaccctcta aatcgaaac 
      gaccccgaa gagtgctgc cacagaccg ccccacctt 
      aaacacccc tactgtaac gaaataatc atccccgaa 
      gacgattac ttctaccga ttattcaac gtaaaatgc 
      ttggacttt gttcgtgct ttcccggcc gctagacct 
      ggttgtaga ctcggttcc cgagttcca tacaacact 
      ttaactggt gttctcgac ggtaacacc gtatacgga 
      gttacggaa aaattcgca cggaaatta agaaccgga 
      tacggtggt cttctccga atgaaccca gcctttgaa 
      gagtacgga cttaaagac ttgctccct ctcaagcta 
      gacatccct gatgaaggg tgcactaga cccaatagc 
      accatgtac tgctttgaa gccggtgaa attcgagtg 
      aacgagcag ttggtgctc acttgtatg cacactctg 
      atggctcgt gagcacaac cgaattgcc aaaggtttg 
      gctcaggtt aatcctcac tgggatgac gaaacgctg 
      ttccaagaa gctcgtcgc attaacatt gccgaaatt 
      caacacatt acttacaac gagtttctt ccgattctt 
      ttgggcaaa gatgtgatg gctaagttt ggtttgctg 
      ttgcagaaa gacggttat tgggatggt tatgatcat 
      aatgttaat ccgaatgtg attgatgcg ttcgcagct 
      gcggcttac agatttggg cactcgctt cttccaacg 
      gctgtggaa aggtggagc aaggcacac aaatttatt 
      gcctccaaa cgtctctct gatttaatc cgtcgcccc 
      tacgactta taccgtgcc ggcgtcttc gacgagtac 
      ctcatgggt ctgatgaac caagtggcc caagccatg 
      gacgactcc atcacccaa gaggtgacc aaccatctg 
      ttcaaaaaa gtcggcgct cggttcggt atggacttg 
      gtcagtctc aacatgcaa agaggccgc gaattcggc 
      attccgggc tacatggaa ttccggaaa ttctgcggc 
      ctgcccgga gccagcaac tttgacgaa ttgttcggt 
      tccatgccc aacgaaact gtccggaaa tactcgacc 
      atttttgag cacccgagt gatgtcgat ttgtggtcg 
      ggcggagtc tccgaacgc ccccttcct ggaagcatg 
      ttagggccc acttttgcc tgcattatc gccacccag 
      tttagttac tcgaggcga ggtgaccgc ttctggtat 
      gaacttcca aaccaaccc tcatctttc acccctgaa 
      caactccac gaagtcagg aaagccaga ttggctcgt 
      attatatgt gacaacacc gatttgatt gacaccata 
      cagttgtac ccaatggtg ttgccggac catgaaatt 
      aacccgagg gtcccgtgc cgaagtgga gtgatcccg 
      agcatagat ttcagcaaa tgggccgaa ttcccgcac 
      gcaggcttc aattctttt gaccacgtg ttaggaaaa 
      aaataatta attgaaatt aactttatg tacagatgc 
      cattgtatt atttatttt aaaatatgc caagtgata 
      atcttgaaa gcaaatcca cgaactcac aatccgaca 
      agttgcatt aaaattcgt gaaactgat ggctaaata 
      attttgcaa cttgttaga acggttctt ataaattat 
      tgaaatttg cttctgaga ttgtcggta gataaggaa 
      ccatttttc agtcaaaac ttacatcaa aacatttta 
      tttgtaaat aggtcgttt gctaggtca tgctattgt 
      ttcgttgta ttgtgacac ccattatat ttatatacg 
      agtattttt tgtataaat aattaattg catgagtat 
      tctcactta attttctac ctccaaaac cgactcaac 
      ctattctat ttttcttct ttttgtaac agatgatgt 
      ggaatgtat accatagga ttaatgcgc ggatttatt 
      tagtcagac aaacttatc tagtactta acactgtca 
      acaaagtac acgcacact atacttaaa agaaaaaac 
      attaaaatt gatttttgt acatattgt tgtagtaat 
      ttattgaaa actcatgtg tatatgtgt ctatatatt 
      aatatattt ttagataaa cc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY