| General information | |||
| TC031335
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007425.3: 1168789..1242694 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC031335 | |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
|
|---|---|---|
| GO ID | * {{ blurIdResult }} |
|
| Evidence | {{ blurEvidenceResult }} |
|
| Reference | * {{ blurReferenceResult }} |
|
| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04991 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
- >TC031335 RA
MIFNRGIPL SWWLTILQI NLGLTIQYT PVDLTGNGD GECALLLSG PPRSSHFDY SLNLLRGSP LEYNGPHTC ITYDAVNAA YLDARKRIH IAQPHGDWK PEDVATVGE LLLDISINL ARTYGLSYE DIEKGLPLI DTSKTLIRE VCPPYLSNV ECRAGKYRR FDGLCNNLE HPTWGATNT PFTRLIGPL YADGMTAPR ISVTGRNLP LSRVVSRTM HPDEGFHDH AGTVMVIAW GQFIDHDYT LTATPLDPL NRNDPEECC HRPPHLKHP YCNEIIIPE DDYFYRLFN VKCLDFVRA FPAARPGCR LGSRVPYNT LTGVLDGNT VYGVTEKFA RKLRTGYGG LLRMNPAFE EYGLKDLLP LKLDIPDEG CTRPNSTMY CFEAGEIRV NEQLVLTCM HTLMAREHN RIAKGLAQV NPHWDDETL FQEARRINI AEIQHITYN EFLPILLGK DVMAKFGLL LQKDGYWDG YDHNVNPNV IDAFAAAAY RFGHSLLPT AVERWSKAH KFIASKRLS DLIRRPYDL YRAGVFDEY LMGLMNQVA QAMDDSITQ EVTNHLFKK VGARFGMDL VSLNMQRGR EFGIPGYME FRKFCGLPG ASNFDELFG SMPNETVRK YSTIFEHPS DVDLWSGGV SERPLPGSM LGPTFACII ATQFSYSRR GDRFWYELP NQPSSFTPE QLHEVRKAR LARIICDNT DLIDTIQLY PMVLPDHEI NPRVPCRSG VIPSIDFSK WAEFPHAGF NSFDHVLGK K
- >TC031335 RA
atgattttt aacaggggg attcccctg agttggtgg ctcacaata ctccaaatc aacctaggc ttaactata caatacact ccagtggac ctcacggga aatggagat ggggaatgt gcccttctt ctaagtggt cctcctaga agtagtcac tttgactac tccctcaac ttactcaga ggaagtccg ttagaatat aatggtcca cacacttgc atcacctac gacgctgtc aatgctgcg tatcttgat gcccgcaaa aggatacac attgctcaa ccacatggc gactggaag cctgaagat gtggcaact gttggtgaa cttcttctg gacatttcg ataaatctg gcacgaacg tacggtttg agttatgaa gacatcgag aaaggtctc cctctgatt gacactagc aagactttg atccgtgag gtgtgcccc ccttatttg tccaatgtg gaatgtcgt gctggcaaa taccgacga tttgatggc ctttgtaac aatttggag cacccgact tggggggcc accaacact ccgtttaca agactgatc ggcccctta tatgccgac ggcatgaca gccccccgc atcagcgta accggaaga aaccttcct ctatcccgt gtcgtctcc cgcactatg catccagat gaaggtttc cacgatcac gccggtacc gtcatggtc attgcatgg ggacaattt atcgaccat gactacact ctcactgca actccatta gaccctcta aatcgaaac gaccccgaa gagtgctgc cacagaccg ccccacctt aaacacccc tactgtaac gaaataatc atccccgaa gacgattac ttctaccga ttattcaac gtaaaatgc ttggacttt gttcgtgct ttcccggcc gctagacct ggttgtaga ctcggttcc cgagttcca tacaacact ttaactggt gttctcgac ggtaacacc gtatacgga gttacggaa aaattcgca cggaaatta agaaccgga tacggtggt cttctccga atgaaccca gcctttgaa gagtacgga cttaaagac ttgctccct ctcaagcta gacatccct gatgaaggg tgcactaga cccaatagc accatgtac tgctttgaa gccggtgaa attcgagtg aacgagcag ttggtgctc acttgtatg cacactctg atggctcgt gagcacaac cgaattgcc aaaggtttg gctcaggtt aatcctcac tgggatgac gaaacgctg ttccaagaa gctcgtcgc attaacatt gccgaaatt caacacatt acttacaac gagtttctt ccgattctt ttgggcaaa gatgtgatg gctaagttt ggtttgctg ttgcagaaa gacggttat tgggatggt tatgatcat aatgttaat ccgaatgtg attgatgcg ttcgcagct gcggcttac agatttggg cactcgctt cttccaacg gctgtggaa aggtggagc aaggcacac aaatttatt gcctccaaa cgtctctct gatttaatc cgtcgcccc tacgactta taccgtgcc ggcgtcttc gacgagtac ctcatgggt ctgatgaac caagtggcc caagccatg gacgactcc atcacccaa gaggtgacc aaccatctg ttcaaaaaa gtcggcgct cggttcggt atggacttg gtcagtctc aacatgcaa agaggccgc gaattcggc attccgggc tacatggaa ttccggaaa ttctgcggc ctgcccgga gccagcaac tttgacgaa ttgttcggt tccatgccc aacgaaact gtccggaaa tactcgacc atttttgag cacccgagt gatgtcgat ttgtggtcg ggcggagtc tccgaacgc ccccttcct ggaagcatg ttagggccc acttttgcc tgcattatc gccacccag tttagttac tcgaggcga ggtgaccgc ttctggtat gaacttcca aaccaaccc tcatctttc acccctgaa caactccac gaagtcagg aaagccaga ttggctcgt attatatgt gacaacacc gatttgatt gacaccata cagttgtac ccaatggtg ttgccggac catgaaatt aacccgagg gtcccgtgc cgaagtgga gtgatcccg agcatagat ttcagcaaa tgggccgaa ttcccgcac gcaggcttc aattctttt gaccacgtg ttaggaaaa aaataa
- >TC031335 RA
ttttaataa aaagttgtt ttatctaat tacaaaatt acataaaaa ctgaaagaa atatagacc ctgacaccg gttactcta tcatcgagc catggaggg gtaaaaatt ctctctcct ccttagtgc caaagcttt ggttctcgg caccagctg gtttaataa gtgacagtg tatacgatt gaccgaccg tactaaccc acatccgtg ctcttctcg tgaaagaag tattgtaca acgcgcggg tggagtgca ctgtgcagt gctgcaaac tgttttttt ttctttctt tttcaagtg tgccatagg aacttaata atgattttt aacaggggg attcccctg agttggtgg ctcacaata ctccaaatc aacctaggc ttaactata caatacact ccagtggac ctcacggga aatggagat ggggaatgt gcccttctt ctaagtggt cctcctaga agtagtcac tttgactac tccctcaac ttactcaga ggaagtccg ttagaatat aatggtcca cacacttgc atcacctac gacgctgtc aatgctgcg tatcttgat gcccgcaaa aggatacac attgctcaa ccacatggc gactggaag cctgaagat gtggcaact gttggtgaa cttcttctg gacatttcg ataaatctg gcacgaacg tacggtttg agttatgaa gacatcgag aaaggtctc cctctgatt gacactagc aagactttg atccgtgag gtgtgcccc ccttatttg tccaatgtg gaatgtcgt gctggcaaa taccgacga tttgatggc ctttgtaac aatttggag cacccgact tggggggcc accaacact ccgtttaca agactgatc ggcccctta tatgccgac ggcatgaca gccccccgc atcagcgta accggaaga aaccttcct ctatcccgt gtcgtctcc cgcactatg catccagat gaaggtttc cacgatcac gccggtacc gtcatggtc attgcatgg ggacaattt atcgaccat gactacact ctcactgca actccatta gaccctcta aatcgaaac gaccccgaa gagtgctgc cacagaccg ccccacctt aaacacccc tactgtaac gaaataatc atccccgaa gacgattac ttctaccga ttattcaac gtaaaatgc ttggacttt gttcgtgct ttcccggcc gctagacct ggttgtaga ctcggttcc cgagttcca tacaacact ttaactggt gttctcgac ggtaacacc gtatacgga gttacggaa aaattcgca cggaaatta agaaccgga tacggtggt cttctccga atgaaccca gcctttgaa gagtacgga cttaaagac ttgctccct ctcaagcta gacatccct gatgaaggg tgcactaga cccaatagc accatgtac tgctttgaa gccggtgaa attcgagtg aacgagcag ttggtgctc acttgtatg cacactctg atggctcgt gagcacaac cgaattgcc aaaggtttg gctcaggtt aatcctcac tgggatgac gaaacgctg ttccaagaa gctcgtcgc attaacatt gccgaaatt caacacatt acttacaac gagtttctt ccgattctt ttgggcaaa gatgtgatg gctaagttt ggtttgctg ttgcagaaa gacggttat tgggatggt tatgatcat aatgttaat ccgaatgtg attgatgcg ttcgcagct gcggcttac agatttggg cactcgctt cttccaacg gctgtggaa aggtggagc aaggcacac aaatttatt gcctccaaa cgtctctct gatttaatc cgtcgcccc tacgactta taccgtgcc ggcgtcttc gacgagtac ctcatgggt ctgatgaac caagtggcc caagccatg gacgactcc atcacccaa gaggtgacc aaccatctg ttcaaaaaa gtcggcgct cggttcggt atggacttg gtcagtctc aacatgcaa agaggccgc gaattcggc attccgggc tacatggaa ttccggaaa ttctgcggc ctgcccgga gccagcaac tttgacgaa ttgttcggt tccatgccc aacgaaact gtccggaaa tactcgacc atttttgag cacccgagt gatgtcgat ttgtggtcg ggcggagtc tccgaacgc ccccttcct ggaagcatg ttagggccc acttttgcc tgcattatc gccacccag tttagttac tcgaggcga ggtgaccgc ttctggtat gaacttcca aaccaaccc tcatctttc acccctgaa caactccac gaagtcagg aaagccaga ttggctcgt attatatgt gacaacacc gatttgatt gacaccata cagttgtac ccaatggtg ttgccggac catgaaatt aacccgagg gtcccgtgc cgaagtgga gtgatcccg agcatagat ttcagcaaa tgggccgaa ttcccgcac gcaggcttc aattctttt gaccacgtg ttaggaaaa aaataatta attgaaatt aactttatg tacagatgc cattgtatt atttatttt aaaatatgc caagtgata atcttgaaa gcaaatcca cgaactcac aatccgaca agttgcatt aaaattcgt gaaactgat ggctaaata attttgcaa cttgttaga acggttctt ataaattat tgaaatttg cttctgaga ttgtcggta gataaggaa ccatttttc agtcaaaac ttacatcaa aacatttta tttgtaaat aggtcgttt gctaggtca tgctattgt ttcgttgta ttgtgacac ccattatat ttatatacg agtattttt tgtataaat aattaattg catgagtat tctcactta attttctac ctccaaaac cgactcaac ctattctat ttttcttct ttttgtaac agatgatgt ggaatgtat accatagga ttaatgcgc ggatttatt tagtcagac aaacttatc tagtactta acactgtca acaaagtac acgcacact atacttaaa agaaaaaac attaaaatt gatttttgt acatattgt tgtagtaat ttattgaaa actcatgtg tatatgtgt ctatatatt aatatattt ttagataaa cc
