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Gene information
General information
TC011212 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 2108440..2109961
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011212
    iBeetle screen iB_04988

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011212 RA
      MCKQLSIIL FISSIIFAP SKSQFDFLK NLQPPQPPT 
      LPSMDVFLS QLFDVTSNE IRNLANNFN FHDVSHKDI 
      TFILHLPKN KTIVLDPKK PTIEATKPT KIIVHGWQA 
      SGTDEKNSE LAETYHNTG DYHVIAVDW AEHAKKVYV 
      HASSSTKDI GHVIGDFIL EITKKDPKL LENIHLIGH 
      SLGGHVAGF AGQRVAAKT GKKVGRITG LDVAAPMFE 
      VPVKRSADS MLSKDDAEF VDVIHTNIG FLGVSDNIG 
      SADFYVENG GPIQPDCFD PVNIFESFG CSHFKSFEY 
      YLESISGKK YEAVSCRNS IEYHILACN NNRKVIMGE 
      KVPKDAYGA FYLETGSPL PYSKLLDGL PKIRINTPM 
      LRLKVN
    CDS sequence
    • >TC011212 RA
      atgtgcaaa caactctcc ataattctc ttcatctcc 
      tccatcatc ttcgcccca agtaagtca cagttcgac 
      tttttgaaa aacctacaa ccgccccaa cccccaacc 
      ctcccctct atggacgtc ttcctctcg caactcttc 
      gacgtgact tccaacgag atcagaaac ctggccaac 
      aacttcaac ttccacgat gtctcccat aaggacatc 
      accttcatc ctgcacctc cccaaaaac aagaccatc 
      gtcctcgac cccaaaaaa cccacaatc gaggcgacc 
      aagcccacg aagatcatc gttcatggg tggcaagct 
      tccggtact gatgagaag aacagcgaa ttggccgaa 
      acgtaccac aacactggt gattaccac gtgatcgct 
      gtggattgg gccgaacac gcaaaaaag gtctacgtc 
      catgcatcc agcagtacc aaggacatt gggcacgtt 
      attggcgat tttatactt gaaataacg aagaaagac 
      ccgaaattg ttggagaat attcacttg attggccac 
      tcccttggc ggtcacgtg gccggcttt gcgggccag 
      cgagtggct gccaagact ggaaaaaag gtggggagg 
      atcaccgga ttggacgtg gcggcgccc atgtttgaa 
      gttccggtg aaaagaagc gccgactcg atgctgagc 
      aaagatgac gccgaattt gttgatgtt atacacacg 
      aatattggg tttttggga gttagtgat aatataggt 
      tctgccgat ttttacgtg gagaatggg gggccgatt 
      caacccgat tgctttgat ccagtcaat atttttgag 
      tcttttggt tgtagccat tttaaatca ttcgagtac 
      taccttgag tcaatttct gggaaaaaa tacgaggcg 
      gtttcgtgt cgcaactct atagagtac cacattttg 
      gcttgtaat aataaccgc aaagtgatt atgggtgaa 
      aaagtgccc aaagacgcc tatggagcg ttttatctt 
      gaaactgga tctccccta ccatacagt aaactttta 
      gatgggctt ccaaaaatt cggataaac acgcccatg 
      ttacgttta aaagtgaac taa
    mRNA sequence
    • >TC011212 RA
      tttgcaggc tcatcataa acataaagt tccattaat 
      tcgagcaat aaaacaaaa tgaaacgat caaatggaa 
      caatatatc tgaatttat ttcacaaaa atgttacgg 
      ccaattgaa tatgtgtac gaaaatatt aattcaaac 
      cgattttca tcgactttc tatcgagaa aaatccaat 
      aagccttga aggtaacaa ccttcagtt ttatgcaaa 
      aaagatgtg aatgtaacg cacttttgc ataattata 
      gtaaaatta ttagattga attgcacaa agaagtaat 
      tagtcattc aagcaacac catgtgcaa acaactctc 
      cataattct cttcatctc ctccatcat cttcgcccc 
      aagtaagtc acagttcga ctttttgaa aaacctaca 
      accgcccca acccccaac cctcccctc tatggacgt 
      cttcctctc gcaactctt cgacgtgac ttccaacga 
      gatcagaaa cctggccaa caacttcaa cttccacga 
      tgtctccca taaggacat caccttcat cctgcacct 
      ccccaaaaa caagaccat cgtcctcga ccccaaaaa 
      acccacaat cgaggcgac caagcccac gaagatcat 
      cgttcatgg gtggcaagc ttccggtac tgatgagaa 
      gaacagcga attggccga aacgtacca caacactgg 
      tgattacca cgtgatcgc tgtggattg ggccgaaca 
      cgcaaaaaa ggtctacgt ccatgcatc cagcagtac 
      caaggacat tgggcacgt tattggcga ttttatact 
      tgaaataac gaagaaaga cccgaaatt gttggagaa 
      tattcactt gattggcca ctcccttgg cggtcacgt 
      ggccggctt tgcgggcca gcgagtggc tgccaagac 
      tggaaaaaa ggtggggag gatcaccgg attggacgt 
      ggcggcgcc catgtttga agttccggt gaaaagaag 
      cgccgactc gatgctgag caaagatga cgccgaatt 
      tgttgatgt tatacacac gaatattgg gtttttggg 
      agttagtga taatatagg ttctgccga tttttacgt 
      ggagaatgg ggggccgat tcaacccga ttgctttga 
      tccagtcaa tatttttga gtcttttgg ttgtagcca 
      ttttaaatc attcgagta ctaccttga gtcaatttc 
      tgggaaaaa atacgaggc ggtttcgtg tcgcaactc 
      tatagagta ccacatttt ggcttgtaa taataaccg 
      caaagtgat tatgggtga aaaagtgcc caaagacgc 
      ctatggagc gttttatct tgaaactgg atctcccct 
      accatacag taaactttt agatgggct tccaaaaat 
      tcggataaa cacgcccat gttacgttt aaaagtgaa 
      ctaaaataa ttcaaataa aaatgattt tattgtaca 
      atgtcacat aaataaaat attttactc gtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY