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Gene information
General information
TC011210 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 2124434..2130900
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011210
    iBeetle screen iB_04987

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection



    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    Pupal morphology
    legs orientation irregular - Number of animals showing the phenotype: 6

    oblique dorsal thoracic muscles median size broader - Number of animals showing the phenotype: 6

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011210 RA
      MTDDIDPNK NDETPMEQP PPTPTQKTE EAPSTAAPT 
      PKPENGDKT TKTNEQQSN CYCGKERNL NIVELLCAN 
      CSRWFHESC IGYQLGKLV PFMANYIFL CKNCSPTGL 
      ESFKKSQAQ IAQMCVTAI ANLQQGSLK EGSNRLMFS 
      KEKEIIPYI EHHWEALTT TSRRVTQSW HSTVHKALI 
      KDIHVLFIF EESPINGQM YGLINTDLT QIKPNYEAM 
      IKGGTLKVT DMGIQHVAG GVKSRSAKR KFPGDVSGG 
      GKKGRGVDL GTPKLPAHG YPLDHPFNK DGYRYFLAE 
      PDPHAPYRQ EFDESSDWA GKPIPGWLY RTLIPSTVL 
      LALHDRAPQ LKISEDRLA VTGEKGYST VRATHGVTK 
      GTWYWEATI EEMPEGTAT RMGWGQDYA NLQAPLGYD 
      KFGYSWRSR KGTRFHESA GKHYSPGFG EGDTLGFMI 
      VLPQNNKTK LVPNTYKDR PLVKFKSHL YYEDKDHVP 
      ERLKSLKAL PGSKILFFK NGECQGVAF ENIYQGTYY 
      PTVSIHKSA TVSVNFGPN FKCPPGSEY DYKGMHQKA 
      EEAICEQTM ADLIYLTEN EGKLRLDSF VL
    CDS sequence
    • >TC011210 RA
      atgaccgat gacatcgac cccaacaaa aatgacgag 
      actcccatg gaacaacca ccaccgaca ccaacacaa 
      aaaaccgaa gaggcgcca agcacagcc gccccgacg 
      ccaaaaccg gaaaacggc gacaagaca accaaaaca 
      aatgaacaa cagagtaac tgctattgc gggaaagaa 
      cgaaatttg aatattgtc gaactattg tgtgcaaat 
      tgctcgcga tggttccac gagtcctgc attgggtac 
      cagttgggc aagttggta cccttcatg gccaactat 
      attttcttg tgcaagaac tgctcgccc acggggttg 
      gagagtttc aaaaaaagt caagcgcaa attgctcaa 
      atgtgtgtc actgcaatt gcaaatttg caacaaggt 
      agcctgaag gagggctct aacaggttg atgtttagc 
      aaagaaaag gagattata ccatacatt gagcaccat 
      tgggaggcg cttactacc acctcacgc cgcgtgacc 
      caatcttgg cactcaaca gtccataaa gcgcttatt 
      aaagacatc cacgtgttg tttattttt gaggaaagt 
      cccatcaat ggtcaaatg tatggtctg atcaacact 
      gacctcacc caaatcaag cccaactac gaggccatg 
      atcaaaggt ggtactctc aaagtcacc gacatgggc 
      atccagcat gttgccggc ggggtgaag tcccgaagc 
      gccaagcgg aagttcccg ggggacgta agcggcggc 
      gggaaaaaa ggccgaggg gtggacctg gggacgccc 
      aaactaccg gcgcacgga taccccctt gaccacccc 
      tttaacaag gacggctac cggtatttc ctggcagag 
      cctgacccc cacgccccc taccggcaa gagttcgac 
      gagagttca gactgggcg gggaaaccc attcccggc 
      tggctctac aggacgctg atccccagc actgtgctg 
      ctggcgctc cacgacagg gccccccag ctgaaaatc 
      tcagaggac aggctggcc gtcacgggg gagaagggc 
      tattccaca gtcagggcc actcatggg gtaaccaag 
      ggtacatgg tactgggag gcgaccatt gaggagatg 
      cctgaaggc acggccacg agaatgggc tgggggcag 
      gactacgcc aacttgcaa gcccccttg ggctacgac 
      aagttcggc tactcgtgg aggtcccga aaaggcacg 
      aggtttcac gaatcggcg gggaaacac tacagtcca 
      ggatttgga gaaggggac actttgggt ttcatgata 
      gttttgccc caaaacaac aagaccaag ctagtcccg 
      aatacatac aaagacaga cccctcgtt aaattcaaa 
      agtcatctc tattatgag gataaagac cacgtgccc 
      gaacggcta aagtcccta aaagcgctc ccagggagc 
      aaaatattg tttttcaaa aatggggaa tgccaagga 
      gtcgctttt gagaatatt tatcaaggg acgtactat 
      cccaccgtt tcgattcat aaaagtgcg acggtttcg 
      gtcaatttt gggcccaat ttcaagtgc ccccctgga 
      tctgaatac gactataaa gggatgcac caaaaggcg 
      gaagaggcc atttgcgag cagacaatg gccgatctg 
      atctactta actgagaat gaaggaaaa ttacgctta 
      gacagtttc gtattgtga
    mRNA sequence
    • >TC011210 RA
      caaacaaaa aacccgctt cccaactgt caaaacata 
      acctcatct atcaccgtt gccgaaaaa tgaccgatg 
      acatcgacc ccaacaaaa atgacgaga ctcccatgg 
      aacaaccac caccgacac caacacaaa aaaccgaag 
      aggcgccaa gcacagccg ccccgacgc caaaaccgg 
      aaaacggcg acaagacaa ccaaaacaa atgaacaac 
      agagtaact gctattgcg ggaaagaac gaaatttga 
      atattgtcg aactattgt gtgcaaatt gctcgcgat 
      ggttccacg agtcctgca ttgggtacc agttgggca 
      agttggtac ccttcatgg ccaactata ttttcttgt 
      gcaagaact gctcgccca cggggttgg agagtttca 
      aaaaaagtc aagcgcaaa ttgctcaaa tgtgtgtca 
      ctgcaattg caaatttgc aacaaggta gcctgaagg 
      agggctcta acaggttga tgtttagca aagaaaagg 
      agattatac catacattg agcaccatt gggaggcgc 
      ttactacca cctcacgcc gcgtgaccc aatcttggc 
      actcaacag tccataaag cgcttatta aagacatcc 
      acgtgttgt ttatttttg aggaaagtc ccatcaatg 
      gtcaaatgt atggtctga tcaacactg acctcaccc 
      aaatcaagc ccaactacg aggccatga tcaaaggtg 
      gtactctca aagtcaccg acatgggca tccagcatg 
      ttgccggcg gggtgaagt cccgaagcg ccaagcgga 
      agttcccgg gggacgtaa gcggcggcg ggaaaaaag 
      gccgagggg tggacctgg ggacgccca aactaccgg 
      cgcacggat acccccttg accacccct ttaacaagg 
      acggctacc ggtatttcc tggcagagc ctgaccccc 
      acgccccct accggcaag agttcgacg agagttcag 
      actgggcgg ggaaaccca ttcccggct ggctctaca 
      ggacgctga tccccagca ctgtgctgc tggcgctcc 
      acgacaggg ccccccagc tgaaaatct cagaggaca 
      ggctggccg tcacggggg agaagggct attccacag 
      tcagggcca ctcatgggg taaccaagg gtacatggt 
      actgggagg cgaccattg aggagatgc ctgaaggca 
      cggccacga gaatgggct gggggcagg actacgcca 
      acttgcaag cccccttgg gctacgaca agttcggct 
      actcgtgga ggtcccgaa aaggcacga ggtttcacg 
      aatcggcgg ggaaacact acagtccag gatttggag 
      aaggggaca ctttgggtt tcatgatag ttttgcccc 
      aaaacaaca agaccaagc tagtcccga atacataca 
      aagacagac ccctcgtta aattcaaaa gtcatctct 
      attatgagg ataaagacc acgtgcccg aacggctaa 
      agtccctaa aagcgctcc cagggagca aaatattgt 
      ttttcaaaa atggggaat gccaaggag tcgcttttg 
      agaatattt atcaaggga cgtactatc ccaccgttt 
      cgattcata aaagtgcga cggtttcgg tcaattttg 
      ggcccaatt tcaagtgcc cccctggat ctgaatacg 
      actataaag ggatgcacc aaaaggcgg aagaggcca 
      tttgcgagc agacaatgg ccgatctga tctacttaa 
      ctgagaatg aaggaaaat tacgcttag acagtttcg 
      tattgtgac aataatgta caatttttt gt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY